Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 225,710 C→G T266R (ACA→AGA)  murQ → N‑acetylmuramic acid 6‑phosphate etherase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE225,7100CG95.9% 250.4 / 3.3 73T266R (ACA→AGA) murQN‑acetylmuramic acid 6‑phosphate etherase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base G (30/40);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

ACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGAACAACAACTTGCAGA  >  USA300TCH1516_ALE/225572‑225847
                                                                                                                                          |                                                                                                                                         
aCCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATTTGAGCATGAGGTGAAAGGTGCGagag                                                                                                                                         >  2:492100/1‑141 (MQ=255)
           gatGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTAtg                                                                                                                              >  2:520557/1‑141 (MQ=255)
           gatGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTAtg                                                                                                                              >  2:21297/1‑141 (MQ=255)
            atGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGCTGTGAAAGTTGCGAGAGTTATGGGTAtgt                                                                                                                             <  2:355804/141‑1 (MQ=255)
              gTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTAtgtgt                                                                                                                           >  4:184281/1‑141 (MQ=255)
              gTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGAGGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTAtgtgt                                                                                                                           <  1:315472/141‑1 (MQ=255)
                tAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTgg                                                                                                                         <  1:60768/141‑1 (MQ=255)
                 aaaGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGc                                                                                                                        <  1:372264/141‑1 (MQ=255)
                 aaaGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGc                                                                                                                        <  2:265060/141‑1 (MQ=255)
                  aaGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCa                                                                                                                       <  3:265454/141‑1 (MQ=255)
                     cAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCAttt                                                                                                                    >  2:299976/1‑141 (MQ=255)
                        ccAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTa                                                                                                                 >  1:279400/1‑141 (MQ=255)
                        ccAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTa                                                                                                                 >  2:276959/1‑141 (MQ=255)
                        ccAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCAATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTa                                                                                                                 <  1:316911/141‑1 (MQ=255)
                            tCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGga                                                                                                             >  4:121318/1‑141 (MQ=255)
                            tCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGga                                                                                                             >  2:618180/1‑141 (MQ=255)
                               aaaaCTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGgaaga                                                                                                          >  2:635913/1‑141 (MQ=255)
                                 aaCTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGaagaag                                                                                                        <  4:175737/141‑1 (MQ=255)
                                 aaCTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATAGGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGaagaag                                                                                                        <  1:629758/141‑1 (MQ=255)
                                   cTGATCGACCGTTCAGTNCGTATTATTCNAGAANTATNTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGca                                                                                                      >  1:484870/1‑141 (MQ=255)
                                     gATCGACCGTTCAGTGCGTATTATTCAAGAAATATCTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGcaac                                                                                                    <  2:614246/141‑1 (MQ=255)
                                        cGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGcaacacg                                                                                                 >  2:604002/1‑139 (MQ=255)
                                             gTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGt                                                                                            >  1:593800/1‑141 (MQ=255)
                                               tCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGtta                                                                                          >  1:135718/1‑141 (MQ=255)
                                                cAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGttat                                                                                         <  1:436492/141‑1 (MQ=255)
                                                cAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGttat                                                                                         <  2:24501/141‑1 (MQ=255)
                                                cAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGttat                                                                                         <  3:297035/141‑1 (MQ=255)
                                                    gCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTaaa                                                                                     <  1:655820/141‑1 (MQ=255)
                                                         ttattCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATg                                                                                <  2:410755/141‑1 (MQ=255)
                                                          tattCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATgg                                                                               >  2:554532/1‑141 (MQ=255)
                                                            ttCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTg                                                                             >  1:253561/1‑141 (MQ=255)
                                                              cAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGAc                                                                           >  1:145204/1‑141 (MQ=255)
                                                              cAAGAAATATGTGCTATAACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGAc                                                                           <  2:3342/141‑1 (MQ=255)
                                                               aaGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACa                                                                          <  2:332089/141‑1 (MQ=255)
                                                                 gAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACAtt                                                                        <  4:171308/141‑1 (MQ=255)
                                                                  aaaTATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGATTATTAAACAATGGTGACATTg                                                                       >  3:253000/1‑141 (MQ=255)
                                                                    atatGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGtt                                                                     >  2:411252/1‑141 (MQ=255)
                                                                     tatGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTa                                                                    <  1:282510/141‑1 (MQ=255)
                                                                     tatGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTa                                                                    <  1:282469/141‑1 (MQ=255)
                                                                       tgtgCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTaaa                                                                  <  4:90267/141‑1 (MQ=255)
                                                                        gtgCTATCACATATGGTGAAGGAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAAc                                                                 <  2:206292/141‑1 (MQ=255)
                                                                        gtgCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAAc                                                                 <  2:206285/141‑1 (MQ=255)
                                                                        gtgCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAAc                                                                 >  1:407738/1‑141 (MQ=255)
                                                                         tgCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACg                                                                <  1:205804/141‑1 (MQ=255)
                                                                          gCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGa                                                               <  2:177899/141‑1 (MQ=255)
                                                                            tATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGc                                                             <  2:144811/141‑1 (MQ=255)
                                                                            tATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGc                                                             <  1:635913/141‑1 (MQ=255)
                                                                             aTCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCa                                                            <  2:374377/141‑1 (MQ=255)
                                                                               cacaTATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAAt                                                          >  1:130058/1‑141 (MQ=255)
                                                                               cacaTATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAAt                                                          <  1:21297/141‑1 (MQ=255)
                                                                                acaTATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGACGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATc                                                         <  3:233623/141‑1 (MQ=255)
                                                                                   tatGATGAAGCAATGGCGATATATCAGGTATGGGCGCGTTATGTGAAAGTTGTGAGAGTTATGGGTATGTGCAGCATGTCTAAGGAATAAGAAACAAGACGGGTAATAAACATAGTGGACATTGTTAAACGATCGATCata                                                      >  4:91882/1‑139 (MQ=255)
                                                                                     tgatgaAGCAATGGCTTTATATCAGGTATCTGAGCATGATATGAAATTTGCGAGAGTTATGGGTATGTATGGCCTTTCTAAGAAAGAAGCAACAAGCCGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAgaga                                                    <  1:103782/141‑1 (MQ=255)
                                                                                        tgaAGCCATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAg                                                 <  4:66453/141‑1 (MQ=255)
                                                                                           aGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACa                                              <  2:412514/141‑1 (MQ=255)
                                                                                               aTGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCt                                          <  4:218384/141‑1 (MQ=255)
                                                                                                 ggCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTa                                        >  1:403297/1‑141 (MQ=255)
                                                                                                  gCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTag                                       >  4:77562/1‑141 (MQ=255)
                                                                                                     ttATATCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTaggag                                    >  1:435048/1‑141 (MQ=255)
                                                                                                      tataTCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTaggagg                                   >  1:360574/1‑141 (MQ=255)
                                                                                                       atatCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAggg                                  <  2:691355/141‑1 (MQ=255)
                                                                                                        tatCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGa                                 <  1:554532/141‑1 (MQ=255)
                                                                                                         atCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGAt                                <  2:565134/141‑1 (MQ=255)
                                                                                                          tCAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGAtt                               <  1:278549/141‑1 (MQ=255)
                                                                                                           cAGGTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGAttt                              >  3:171515/1‑141 (MQ=255)
                                                                                                              gTATCTGAGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTaaa                           <  1:27329/141‑1 (MQ=255)
                                                                                                                     aGCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCaa                    >  1:442183/1‑141 (MQ=255)
                                                                                                                      gCATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACaaaa                   >  1:310004/1‑141 (MQ=255)
                                                                                                                       cATGATGTGAAAGTTGCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAg                  >  3:275503/1‑141 (MQ=255)
                                                                                                                         tgatgTGAAAGTTGCGAGAGTTATGGGTATGTGTGGGATTTCTAAGGAAGAAGCAACTAGATGGTTATTAAACAATGGTCACATTGTTAAACGAGCAGTCAGAGATAGACAACCTTAGGAGAGATTTAAATGACCTAAGaa                <  4:149070/141‑1 (MQ=255)
                                                                                                                         tgatgTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTCAGGTGGGGCTTAAATGACCAAAGaa                >  1:76653/1‑141 (MQ=255)
                                                                                                                                      gCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGAACAACAACTTGCAg   >  2:576009/1‑141 (MQ=255)
                                                                                                                                      gCGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGAACAACAACTTGCAg   >  2:651830/1‑141 (MQ=255)
                                                                                                                                       cGAGAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGAACAACAACTTGCAGa  <  2:279400/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
ACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCGTTATATCAGGTATCTGAGCATGATGTGAAAGTTGCGACAGTTATGGGTATGTGTGGCATTTCTAAGGAAGAAGCAACAAGACGGTTATTAAACAATGGTGACATTGTTAAACGAGCAATCAGAGATAGACAACCTTAGGAGGGATTTAAATGACCAAAGAACAACAACTTGCAGA  >  USA300TCH1516_ALE/225572‑225847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: