Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 188,567 A→T 5.0% D143V (GAT→GTT)  wecC → UDP‑N‑acetyl‑D‑mannosamine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE188,5670AT 5.0% 228.8 / 2.5 83D143V (GAT→GTT) wecCUDP‑N‑acetyl‑D‑mannosamine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (44/33);  new base T (2/2);  total (47/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

TTATGCGTGCATTAGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGCACATTC  >  USA300TCH1516_ALE/188429‑188700
                                                                                                                                          |                                                                                                                                     
ttATGCGTGCATTAGATAGTATTTTAGCATTTTTAGAAAAAGGAAATACCATTTAGCCAGATTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGat                                                                                                                                      <  1:166576/140‑1 (MQ=255)
  aTGCGTGCATTAGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGatat                                                                                                                                    <  1:176524/140‑1 (MQ=255)
  aTGCGTGCATTAGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGatat                                                                                                                                    <  1:632863/140‑1 (MQ=255)
    gCGTGCATTAGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCATAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGAtattt                                                                                                                                  <  1:592835/140‑1 (MQ=255)
        gCATTAGATAGTATTTTATCATTTCTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATCATTTTGTAAAACCAGTATTTGTAAATGTAGGGTTTACAATAGGTGAAGATatttattt                                                                                                                              <  1:717079/140‑1 (MQ=255)
         cATTAGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATAtttattta                                                                                                                             >  1:75259/1‑140 (MQ=255)
         cATTAGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTAAAAAATTAGGGTTTACAATAGGTGAAGATAtttattta                                                                                                                             >  1:68289/1‑140 (MQ=255)
           ttAGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGt                                                                                                                           <  2:527403/140‑1 (MQ=255)
           ttAGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGACATTTCTTTAgg                                                                                                                           <  2:556908/140‑2 (MQ=255)
           ttAGATAGTATTTGATCATTTTTAGAAAAAGGATATACCATGATGTTAGAGTCGACATGTGCGCATATCACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATATGTGAAGGTATTTATTTAGt                                                                                                                           <  2:125596/140‑1 (MQ=255)
            tAGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGTGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTg                                                                                                                          <  2:441481/140‑1 (MQ=255)
            tAGAAAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATCGCGCCTAAAACGATGGATGATTTTGTAAAAACAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTg                                                                                                                          <  2:346417/140‑1 (MQ=255)
             aGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGc                                                                                                                         >  1:622651/1‑140 (MQ=255)
             aGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGc                                                                                                                         >  1:618363/1‑140 (MQ=255)
             aGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGc                                                                                                                         >  2:601928/1‑140 (MQ=255)
             aGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTAGACAATTGCGCCTAAAACGAAGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTAAAATAGGAGAAGATATTTAATAAGTGc                                                                                                                         >  2:343049/1‑140 (MQ=255)
               aTAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAACATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCAt                                                                                                                       >  2:397331/1‑140 (MQ=255)
                  gTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCGGTCATTGAAAATTTAGGGTTTACAATAGGTGAACATATTTATTTAGTGCATTGt                                                                                                                    <  1:471218/140‑1 (MQ=255)
                  gTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGt                                                                                                                    >  2:672213/1‑140 (MQ=255)
                      tttATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGTGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAg                                                                                                                <  1:497471/140‑1 (MQ=255)
                          tCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACg                                                                                                            <  2:219516/140‑1 (MQ=255)
                          tCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACg                                                                                                            >  2:711636/1‑140 (MQ=255)
                           cATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACgt                                                                                                           >  1:459949/1‑140 (MQ=255)
                            aTTTTTAGAAACAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGAGGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACgtg                                                                                                          >  1:124506/1‑140 (MQ=255)
                            aTTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGGACgtg                                                                                                          >  2:19027/1‑140 (MQ=255)
                                  aGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGc                                                                                                    >  2:554456/1‑140 (MQ=255)
                                   gAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGca                                                                                                   >  2:321116/1‑139 (MQ=255)
                                    aaaaaGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCa                                                                                                  >  1:3562/1‑140 (MQ=255)
                                     aaaaGGAAATACCATTATTGTAGAGTCGACAATTGCGCCGAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATATGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAg                                                                                                 <  2:380598/140‑1 (MQ=255)
                                       aaGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGa                                                                                               <  2:309839/140‑1 (MQ=255)
                                         ggAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGaaa                                                                                             <  1:409086/140‑1 (MQ=255)
                                         ggAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGaaa                                                                                             <  1:624522/140‑1 (MQ=255)
                                          gAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTTTAGTGCCAGGacaa                                                                                            >  2:308995/1‑140 (MQ=255)
                                           aaaTACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTAAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGaaaaa                                                                                           >  1:32889/1‑140 (MQ=255)
                                              tACCATTTTTGTAGTGTCGACAGTTGCGCCTTTAACGATGGATGATTTTGTATATCTAGTCATTGATAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAAttt                                                                                        <  1:317388/140‑1 (MQ=255)
                                              tACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAAatt                                                                                        >  1:546248/1‑140 (MQ=255)
                                              tACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGAGAAGATATTTAGTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAAttt                                                                                        >  2:55048/1‑140 (MQ=255)
                                                ccatacttGAAGAGTCGACAATTGCGCCTAAAACGATTGATGATTTGGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTa                                                                                      <  2:325977/134‑1 (MQ=255)
                                                ccATTATTTGAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTa                                                                                      <  2:631605/140‑1 (MQ=255)
                                                 cATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGATCGTGTACTGCCAGGAAAAAATTTag                                                                                     >  1:446879/1‑140 (MQ=255)
                                                 cATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACATGTACTGCCAGGAAAAATtataa                                                                                     >  1:309915/1‑139 (MQ=255)
                                                 cATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCAAGAACGAATAATGCCAGAATAAATTTTac                                                                                     >  1:70611/1‑139 (MQ=255)
                                                 cATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGGTTACAATAGGTGAAGATATTTATTTAATGCATTGTACAGAACGTGTACTGCCAGGAAAAAATTTaa                                                                                     >  1:85219/1‑139 (MQ=255)
                                                   ttattGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTagaa                                                                                   >  1:68755/1‑140 (MQ=255)
                                                      ttGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACAAGTCATTGAAAATGTAGGGTATAGAATAGGTGAAGATATTTATTTAGTGCATTCTCCAGAACGTGGACTGCCAGGAAAAATTTTAgaagaa                                                                                >  2:621697/1‑140 (MQ=255)
                                                          agagTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAACATTTAGGGTTTACAATAGGTGAAGGTATTTATTTAGTGCATTGTTCAGAACGTGTACTGCCAGGAAAAATTTTATAAGAATTAg                                                                            >  2:107292/1‑140 (MQ=255)
                                                           gagTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGACTTCGt                                                                           >  2:24629/1‑140 (MQ=255)
                                                           gagTCGACAATTGAGACTAAAACGTTGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGt                                                                           >  2:625423/1‑140 (MQ=255)
                                                           gagTAGACAATTGCGCCTAAAACGATGGATGATTTTGTAAGACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTGTAGAAGAATTAGt                                                                           >  1:518631/1‑140 (MQ=255)
                                                              tCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCa                                                                        >  2:264995/1‑140 (MQ=255)
                                                               cGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCAt                                                                       >  1:643342/1‑140 (MQ=255)
                                                                gACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGTAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATa                                                                      <  1:415341/140‑1 (MQ=255)
                                                                gACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATa                                                                      <  1:109449/140‑1 (MQ=255)
                                                                 aCAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGTTATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATaa                                                                     >  2:164505/1‑140 (MQ=255)
                                                                 aCAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGTTATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATaa                                                                     >  2:164711/1‑140 (MQ=255)
                                                                          ccTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTAt                                                            >  1:634049/1‑140 (MQ=255)
                                                                                       atgatTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGtgtg                                               >  2:39465/1‑140 (MQ=255)
                                                                                        tgaaTTTGTAACACCATGCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGGCCAGAACGTGTACTGCCATGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGa                                              <  2:73589/140‑1 (MQ=255)
                                                                                           ttttGGAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTg                                           <  2:162634/140‑1 (MQ=255)
                                                                                                tAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTTCAGAACGTGTACTGTCTGGTAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCt                                      <  1:548172/140‑1 (MQ=255)
                                                                                                tAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCt                                      >  1:404432/1‑140 (MQ=255)
                                                                                                  aaaCCATTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTATTTCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAATAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTg                                    <  1:438217/140‑1 (MQ=255)
                                                                                                    aCCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTa                                  >  2:614511/1‑140 (MQ=255)
                                                                                                     ccAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTAt                                 <  1:58381/140‑1 (MQ=255)
                                                                                                     ccAGTCATTGAAAATTTAGAGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAAAAATAGTATCATTGGTCGTGTGACTGAAGTCTGTAt                                 >  2:218512/1‑140 (MQ=255)
                                                                                                     ccAGTCATTGAAAAATTAGGGATTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGTTGTGACTGAAGCTTGTAt                                 >  1:379317/1‑140 (MQ=255)
                                                                                                           aTTGAAAATTTAGGGTTTACAATAGGGGAAGATATTTATTTAGTGCATTGTCCAGAAGGTGTACGGCCAGGAAAAAATTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTCAGACTGCAGCTTGTATTaaacc                           >  2:113380/1‑138 (MQ=255)
                                                                                                            ttGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAAAAGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCg                          >  1:8591/1‑140 (MQ=255)
                                                                                                             tGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCgg                         >  2:520739/1‑140 (MQ=255)
                                                                                                              gAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCggg                        <  2:87995/140‑1 (MQ=255)
                                                                                                               aaaaTTTAGCGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGGGACTGAAGCTTGTATTGAAGCggga                       >  1:532795/1‑139 (MQ=255)
                                                                                                                      aGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACgtg                <  1:601928/140‑1 (MQ=255)
                                                                                                                              cAATAGGTGAAGATATTTATTTGGTGCATTGTCCAGAACGTGTACTGCCAGGAGAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGc        <  2:618363/140‑1 (MQ=255)
                                                                                                                              cAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGc        >  1:613551/1‑140 (MQ=255)
                                                                                                                                aTAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGcac      <  2:365025/140‑1 (MQ=255)
                                                                                                                                 tAGGTGAAGTTATTTATTTAGTGCATTGTCCAGAACGTTTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGcaca     <  1:164711/140‑1 (MQ=255)
                                                                                                                                 tAGGTGAAGTTATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGcaca     <  1:164505/140‑1 (MQ=255)
                                                                                                                                 tAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGcaca     <  1:672213/140‑1 (MQ=255)
                                                                                                                                 tAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGcaca     <  2:622651/140‑1 (MQ=255)
                                                                                                                                 tAGGTGAAGATATTTACTTAGTGCATTTTCCAGTACGTGTAATGGGCTTAAAAGTTTTAGATGAATTAGTTTATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGcaca     <  1:343049/140‑1 (MQ=255)
                                                                                                                                  aGGTGAAGATTTTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGCGTCTATCGCACAt    <  1:100160/140‑1 (MQ=255)
                                                                                                                                  aGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGCACAt    >  1:590182/1‑140 (MQ=255)
                                                                                                                                  aGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGCACAt    <  1:577004/140‑1 (MQ=255)
                                                                                                                                  aGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGCACAt    <  1:405672/140‑1 (MQ=255)
                                                                                                                                   ggTGAAGATATTTATTTCGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTGGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGAACAtt   >  1:103297/1‑140 (MQ=255)
                                                                                                                                    gTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATATCAATCGTATCATTGGCGTTGTGACTGAAGATTGTATTAAAGCGGGTAAACGTTTCTATCGCACATTc  >  1:35641/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                     
TTATGCGTGCATTAGATAGTATTTTATCATTTTTAGAAAAAGGAAATACCATTATTGTAGAGTCGACAATTGCGCCTAAAACGATGGATGATTTTGTAAAACCAGTCATTGAAAATTTAGGGTTTACAATAGGTGAAGATATTTATTTAGTGCATTGTCCAGAACGTGTACTGCCAGGAAAAATTTTAGAAGAATTAGTTCATAACAATCGTATCATTGGCGGTGTGACTGAAGCTTGTATTGAAGCGGGTAAACGTGTCTATCGCACATTC  >  USA300TCH1516_ALE/188429‑188700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑