BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,710,898	(T)_6→5	coding (29/1812 nt)	oatA_2 ←	O‑acetyltransferase OatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,710,893	0	T	.	91.5%	369.5 / 26.5	94	coding (34/1812 nt)	oatA_2	O‑acetyltransferase OatA
Reads supporting (aligned to +/- strand): ref base T (4/4); new base . (44/42); total (48/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 104 total aligned reads displayed.

TAACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCTTAATT  >  USA300TCH1516_ALE/2710754‑2711030

tAACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACAtt                                                                                                                                          <  1:173388/141‑1 (MQ=255)

tAACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACAtt                                                                                                                                          <  4:261772/141‑1 (MQ=255)

 aaCCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACAttt                                                                                                                                         >  2:209554/1‑141 (MQ=255)

 aaCCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACAttt                                                                                                                                         >  2:443165/1‑141 (MQ=255)

 aaCCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACAttt                                                                                                                                         >  2:703283/1‑141 (MQ=255)

    cTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACAtttttc                                                                                                                                      <  2:611555/141‑2 (MQ=255)

     tGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACAtttttct                                                                                                                                     <  2:515144/141‑3 (MQ=255)

      gAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACAtttttcta                                                                                                                                    >  1:729918/1‑138 (MQ=255)

       aaaTAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACAtttttctaa                                                                                                                                   >  1:384694/1‑137 (MQ=255)

       aaaGAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACAtttttctaa                                                                                                                                   <  2:250184/141‑5 (MQ=255)

           aaCGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGt                                                                                                                              >  1:466817/1‑141 (MQ=255)

             cGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGtt                                                                                                                             >  3:78548/1‑141 (MQ=255)

             cGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGttt                                                                                                                            >  1:37442/1‑141 (MQ=255)

              gaagaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTa                                                                                                                           <  1:651222/141‑1 (MQ=255)

              gaagaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTa                                                                                                                           <  2:419870/141‑1 (MQ=255)

                agaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTaaa                                                                                                                         >  2:470896/1‑141 (MQ=255)

                agaaTGTATCTACCCCTAAAAAGCCCCCCCTTAACCATTGTGCATTCAAGTGATAACTAAAGATTCCTATCACTGCGAACGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTaaa                                                                                                                         <  3:34575/141‑1 (MQ=255)

                  aaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGt                                                                                                                       <  4:118082/141‑1 (MQ=255)

                      tATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCttt                                                                                                                   >  4:85148/1‑141 (MQ=255)

                        tCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTTTAAAGTCtttt                                                                                                                  >  2:334132/1‑141 (MQ=255)

                        tCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTg                                                                                                                 >  1:98315/1‑141 (MQ=255)

                               cTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCAt                                                                                                          >  3:325349/1‑141 (MQ=255)

                               cTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCAt                                                                                                          <  1:334103/141‑1 (MQ=255)

                                     aGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTtaat                                                                                                    <  2:25459/141‑1 (MQ=255)

                                        ccccACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATaaa                                                                                                 >  1:784749/1‑141 (MQ=255)

                                        ccccACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAAGCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATaaa                                                                                                 <  4:164675/141‑1 (MQ=255)

                                        ccccACTTAACCATTGGGCATGCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTCTCCATGTTAATaaa                                                                                                 <  4:66747/141‑1 (MQ=255)

                                            aCTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGcc                                                                                             >  3:51978/1‑141 (MQ=255)

                                              ttAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGccc                                                                                            <  2:208810/141‑1 (MQ=255)

                                                aaCCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCa                                                                                          >  1:162040/1‑141 (MQ=255)

                                                 aCCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCatt                                                                                        <  1:454294/141‑1 (MQ=255)

                                                 aCCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCatt                                                                                        >  3:135268/1‑141 (MQ=255)

                                                      tgtgCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCatttattt                                                                                   <  1:32628/141‑1 (MQ=255)

                                                       gtgCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTGTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCatttattt                                                                                   >  3:108394/1‑141 (MQ=255)

                                                       gtgCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTAtttt                                                                                  >  1:647299/1‑141 (MQ=255)

                                                       gtgCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAAAGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTAtttt                                                                                  >  2:490529/1‑141 (MQ=255)

                                                        tgCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTAttttt                                                                                 <  2:644860/141‑1 (MQ=255)

                                                        tgCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTAttttt                                                                                 <  1:425696/141‑1 (MQ=255)

                                                         gCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtc                                                                                <  3:319971/141‑1 (MQ=255)

                                                         gCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtc                                                                                <  1:808369/141‑1 (MQ=255)

                                                          cATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtct                                                                               <  2:329289/141‑1 (MQ=255)

                                                           aTTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtctc                                                                              <  1:587507/141‑1 (MQ=255)

                                                           aTTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtctc                                                                              <  3:848/141‑1 (MQ=255)

                                                           aTTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtctc                                                                              <  2:348318/141‑1 (MQ=255)

                                                            ttCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTGAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTACAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTctct                                                                             >  1:860085/1‑141 (MQ=255)

                                                            ttCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTctct                                                                             >  2:202574/1‑141 (MQ=255)

                                                            ttCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTctct                                                                             >  2:804241/1‑141 (MQ=255)

                                                            ttCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTctct                                                                             <  1:91060/141‑1 (MQ=255)

                                                               aaGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTAtt                                                                          >  1:333887/1‑141 (MQ=255)

                                                               aaGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTAtt                                                                          <  1:353350/141‑1 (MQ=255)

                                                               aaGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTAtt                                                                          <  2:449030/141‑1 (MQ=255)

                                                                 gTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTAtttt                                                                        >  4:235435/1‑141 (MQ=255)

                                                                  tGATAAATAATGATTCCTATAACTGCGAACGCTCCCTGACCAGGGTATCCATTTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTg                                                                       <  3:70003/141‑1 (MQ=255)

                                                                      aaataaTGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGc                                                                   <  2:305636/141‑1 (MQ=255)

                                                                        ataatGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCat                                                                 >  1:682580/1‑141 (MQ=255)

                                                                           atGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATc                                                              >  1:267632/1‑141 (MQ=255)

                                                                           atGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATc                                                              <  1:708324/141‑1 (MQ=255)

                                                                             gATTCCTATAACTGCGAATTCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTTTTTTTTTTATCATATcac                                                            >  4:34615/1‑141 (MQ=255)

                                                                             gATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATcac                                                            >  2:786603/1‑141 (MQ=255)

                                                                             gATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATcac                                                            <  3:98669/141‑1 (MQ=255)

                                                                              aTTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATcaca                                                           <  4:41044/141‑1 (MQ=255)

                                                                               ttCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACaa                                                          >  1:135664/1‑141 (MQ=255)

                                                                               ttCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACaa                                                          >  4:351948/1‑141 (MQ=255)

                                                                                tCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAat                                                         >  2:67070/1‑141 (MQ=255)

                                                                                tCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAat                                                         >  3:267493/1‑141 (MQ=255)

                                                                                 ccTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAat                                                         <  4:78548/141‑1 (MQ=255)

                                                                                  cTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAatat                                                       >  1:172629/1‑141 (MQ=255)

                                                                                  cTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAAAACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAatat                                                       >  1:440564/1‑141 (MQ=255)

                                                                                          gCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaa                                               >  1:542860/1‑141 (MQ=255)

                                                                                           cGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaa                                              >  2:549364/1‑141 (MQ=255)

                                                                                           cGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaa                                              >  1:609854/1‑141 (MQ=255)

                                                                                           cGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaa                                              >  2:448161/1‑141 (MQ=255)

                                                                                           cGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCACATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaa                                              >  1:367083/1‑141 (MQ=255)

                                                                                            gAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaat                                             >  3:124370/1‑141 (MQ=255)

                                                                                            gAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaat                                             >  1:207354/1‑141 (MQ=255)

                                                                                            gAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaat                                             >  4:213227/1‑141 (MQ=255)

                                                                                            gAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaat                                             <  2:700373/141‑1 (MQ=255)

                                                                                            gAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTATCTATTTTGTAGCATATCACAATAttttattttca                                             <  2:466817/141‑8 (MQ=255)

                                                                                             aaTGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaata                                            >  1:383212/1‑141 (MQ=255)

                                                                                             aaTGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaata                                            >  2:178560/1‑141 (MQ=255)

                                                                                                gCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaataaa                                          <  3:42961/141‑1 (MQ=255)

                                                                                                gCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaataaaa                                         <  1:341598/141‑1 (MQ=255)

                                                                                                 cccTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaataaaat                                        >  2:339722/1‑141 (MQ=255)

                                                                                                    tCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATAtt                                     >  2:435615/1‑141 (MQ=255)

                                                                                                        tCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCAc                                 <  2:691487/141‑1 (MQ=255)

                                                                                                        tCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCAc                                 <  2:335148/141‑1 (MQ=255)

                                                                                                           aTCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGa                              <  4:20224/141‑1 (MQ=255)

                                                                                                             cTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGAtt                            >  1:99415/1‑141 (MQ=255)

                                                                                                                  ccAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCAt                       <  1:443165/141‑1 (MQ=255)

                                                                                                                  ccAGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCAt                       <  1:52602/141‑1 (MQ=255)

                                                                                                                    aGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTa                     <  1:310307/141‑1 (MQ=255)

                                                                                                                    aGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTa                     >  2:779888/1‑141 (MQ=255)

                                                                                                                    aGGTAAGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTACCCATGTTAGTAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTa                     >  3:283108/1‑141 (MQ=255)

                                                                                                                        aaGTATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTg                 <  4:206208/141‑1 (MQ=255)

                                                                                                                           tATCGCGGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGAtt              <  2:211864/141‑1 (MQ=255)

                                                                                                                               gcgGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAAc          <  3:315896/141‑1 (MQ=255)

                                                                                                                               gcgGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAAc          >  2:540413/1‑141 (MQ=255)

                                                                                                                                cgGGGAATACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAAcc         <  2:41639/141‑1 (MQ=255)

                                                                                                                                      aTACA‑TTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCttaat   >  1:792731/1‑141 (MQ=255)

                                                                                                                                       tACATTTTTT‑TAAACGTTTAAAGTCTTTTGTATCCATGTTAATACACGCCCCATTTATTTTTCTATATTTTGTAGCATATCACAATATTTTTGATAATAAAATATTGCACTGTTTTTCATTAATTGATTTAACCCttaatt  <  2:745010/141‑1 (MQ=255)

TAACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCTTAATT  >  USA300TCH1516_ALE/2710754‑2711030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`