BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,419,527	G→T	P7T (CCT→ACT)	USA300HOU_RS07040 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,419,527	0	G	T	98.0%	320.1 / ‑0.1	102	P7T (CCT→ACT)	USA300HOU_RS07040	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (63/36); total (64/38)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

Only 100 of 102 total aligned reads displayed.

TTTGGAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGGCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATCTACTAATAGAAAAATCATT  >  USA300TCH1516_ALE/1419394‑1419663

tttGGAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCg                                                                                                                                   >  2:418063/1‑141 (MQ=255)

tttGGAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCg                                                                                                                                   <  1:773370/141‑1 (MQ=255)

tttGGAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCg                                                                                                                                   <  1:557829/141‑1 (MQ=255)

 ttGGAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGa                                                                                                                                  >  2:258919/1‑141 (MQ=255)

 ttGGAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGa                                                                                                                                  >  4:167712/1‑141 (MQ=255)

  tGGAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGaa                                                                                                                                 >  2:353404/1‑141 (MQ=255)

  tGGAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGaa                                                                                                                                 >  1:561763/1‑141 (MQ=255)

  tGGAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGaa                                                                                                                                 >  4:24424/1‑141 (MQ=255)

  cggAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACGATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCGATAAATAATTAGTCATATCGaa                                                                                                                                 >  2:283946/2‑141 (MQ=255)

   ggAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGaat                                                                                                                                >  2:28735/1‑141 (MQ=255)

    gAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGaata                                                                                                                               >  2:807335/1‑141 (MQ=255)

    gAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGaata                                                                                                                               <  2:91518/141‑1 (MQ=255)

    gAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGaata                                                                                                                               <  3:294190/141‑1 (MQ=255)

    gAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGaata                                                                                                                               <  1:683846/141‑1 (MQ=255)

      aTAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGaataat                                                                                                                             >  2:332946/1‑141 (MQ=255)

      aTAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGaataat                                                                                                                             >  1:289432/1‑141 (MQ=255)

          aaaaTGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCa                                                                                                                         <  1:286709/141‑1 (MQ=255)

                cAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATattca                                                                                                                   <  2:413539/141‑1 (MQ=255)

                cAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATattca                                                                                                                   >  4:47924/1‑141 (MQ=255)

                 aaGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATattcat                                                                                                                  >  1:461847/1‑141 (MQ=255)

                  aGCACACCTAATATAACAAATGCAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTTATAAAATCATTATTAATGTTGTGATCCATAATTAATTATTCATATCGAATAATGTCATattcatt                                                                                                                 >  2:28278/1‑141 (MQ=255)

                   gCACACCTAATATAACAAATGTAATCACTGGAGATACAAACCAGTTATATAAAACTAAGCAACAGAATATGGTTAATAAAATCATTATTAATGTGGTGATCCATAAATAATTAGTAATATCGAATAATGTCATattcatta                                                                                                                >  2:151581/1‑140 (MQ=255)

                              ataACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTAttt                                                                                                     >  4:325925/1‑141 (MQ=255)

                               taACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTc                                                                                                    <  1:609038/141‑1 (MQ=255)

                               taACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTc                                                                                                    >  2:663305/1‑141 (MQ=255)

                                aaCAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCa                                                                                                   >  1:843867/1‑141 (MQ=255)

                                  cAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCAtt                                                                                                 >  2:348817/1‑141 (MQ=255)

                                  cAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCAtt                                                                                                 >  1:844040/1‑141 (MQ=255)

                                  cAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCAtt                                                                                                 >  4:367482/1‑141 (MQ=255)

                                  cAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGGCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCAtt                                                                                                 <  3:208393/141‑1 (MQ=255)

                                    aaTGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTAc                                                                                               >  1:283061/1‑141 (MQ=255)

                                       gTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACATAATATTTTTAATAAAATCATTATTAATGTTTTGATCCATAAATAATTAGTCAGATGCAATAATGTTATATTCATTTCCCTTATATTTCATTACttt                                                                                            >  4:155334/1‑141 (MQ=255)

                                        tAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTc                                                                                           >  1:137272/1‑141 (MQ=255)

                                            tACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTg                                                                                       >  1:172557/1‑141 (MQ=255)

                                             aCTTCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATTGAATAATGTCATATTCATTATCATTTTATTTCATTACTTTCTTTGt                                                                                      >  4:249127/1‑141 (MQ=255)

                                              cTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGta                                                                                     <  1:767425/141‑1 (MQ=255)

                                              cTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGta                                                                                     <  3:170978/141‑1 (MQ=255)

                                              cTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGta                                                                                     >  4:59400/1‑141 (MQ=255)

                                                gCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGtata                                                                                   <  2:617966/141‑1 (MQ=255)

                                                 cAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATAc                                                                                  <  2:775/141‑1 (MQ=255)

                                                      aCAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACAtttt                                                                             >  1:494152/1‑141 (MQ=255)

                                                        aaaCCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTttat                                                                           <  1:397707/141‑1 (MQ=255)

                                                         aaCCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTttatt                                                                          >  4:143634/1‑141 (MQ=255)

                                                         aaCCATGTATTTAAAACTAAGCAACAGAATATTGGTGATAAGATCATTATTAATGTGGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTAATTTCATTACGTTGGTTTTATACGTTttatt                                                                          >  4:34521/1‑141 (MQ=255)

                                                          aCCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTttatta                                                                         >  2:396869/1‑141 (MQ=255)

                                                             atttatttAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATaa                                                                      >  2:815059/1‑141 (MQ=255)

                                                               ttatttaAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAAt                                                                    <  1:749144/141‑1 (MQ=255)

                                                                 atttaAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAAttt                                                                  >  2:146636/1‑141 (MQ=255)

                                                                  tttaAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAAtttt                                                                 <  2:503438/141‑1 (MQ=255)

                                                                  tttaAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAAtttt                                                                 >  4:249731/1‑141 (MQ=255)

                                                                  tttaAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAAtttt                                                                 >  4:139646/1‑141 (MQ=255)

                                                                   ttaAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATCATGACATTTTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAAttttt                                                                >  3:190569/1‑141 (MQ=255)

                                                                     aaaaCTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGGCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATATATaa                                                              <  2:311456/141‑1 (MQ=255)

                                                                        aCTAAGCAACAGAATATTGTCAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTaaaaa                                                           <  1:497737/141‑1 (MQ=255)

                                                                         cTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAAc                                                          <  1:247061/141‑1 (MQ=255)

                                                                          tAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACt                                                         <  1:258919/141‑1 (MQ=255)

                                                                            aGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTa                                                       >  1:187030/1‑141 (MQ=255)

                                                                              cAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTaaa                                                     >  1:527223/1‑141 (MQ=255)

                                                                               aaCAGACTATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTaacc                                                    <  2:608678/141‑3 (MQ=255)

                                                                               aaCAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATAAGAAATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTaaaa                                                    >  1:235256/1‑140 (MQ=255)

                                                                                aCAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACa                                                   <  2:41967/141‑1 (MQ=255)

                                                                                aCAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACa                                                   >  2:236780/1‑141 (MQ=255)

                                                                                aCAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACa                                                   >  1:322444/1‑141 (MQ=255)

                                                                                      tatTGTTAATAAAATCATTATTAATGTTGTGACCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGca                                             <  2:691256/141‑1 (MQ=255)

                                                                                        ttGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGcagt                                           <  2:494152/141‑1 (MQ=255)

                                                                                        ttGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGcagt                                           <  2:691032/141‑1 (MQ=255)

                                                                                         tGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGcagtc                                          <  4:129599/141‑1 (MQ=255)

                                                                                          gTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGcagtcg                                         >  4:301904/1‑140 (MQ=255)

                                                                                            taataaAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGcagtcagt                                       <  1:627707/141‑1 (MQ=255)

                                                                                             aataaAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGtt                                      >  3:23508/1‑141 (MQ=255)

                                                                                              ataaAATCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGttt                                     <  1:348817/141‑1 (MQ=255)

                                                                                                  aaTCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAg                                 >  4:180328/1‑141 (MQ=255)

                                                                                                  aaTCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAg                                 <  2:328615/141‑1 (MQ=255)

                                                                                                  aaTCATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAg                                 <  2:238672/141‑1 (MQ=255)

                                                                                                     cATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCaa                              <  2:325188/141‑1 (MQ=255)

                                                                                                     cATTATTAATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCaa                              >  4:186767/1‑141 (MQ=255)

                                                                                                        tattaATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAatat                           <  2:361264/141‑1 (MQ=255)

                                                                                                         attaATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATAtt                          <  2:391268/141‑1 (MQ=255)

                                                                                                          ttaATGTTGTTATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATtc                         <  2:857431/141‑1 (MQ=255)

                                                                                                           taATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATtct                        >  1:767174/1‑141 (MQ=255)

                                                                                                           taATGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATtct                        >  2:308959/1‑141 (MQ=255)

                                                                                                             aTGTTGTGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATtctat                      >  4:274651/1‑141 (MQ=255)

                                                                                                              tgttgtGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATtctatc                     >  3:107116/1‑141 (MQ=255)

                                                                                                                ttgtGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTaaaaa                                                           >  1:551424/1‑101 (MQ=255)

                                                                                                                ttgtGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTATATTTCATTACTTTACTTGTATAAATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTAAAGCAATATtctatcaa                   >  2:109543/1‑139 (MQ=255)

                                                                                                                ttgtGATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTACTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTaaaaa                                                           <  2:551424/101‑1 (MQ=255)

                                                                                                                 tgtgATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATctac                  >  4:103849/1‑141 (MQ=255)

                                                                                                                  gtgATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATctact                 >  4:66225/1‑141 (MQ=255)

                                                                                                                  gtgATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATctact                 >  3:271239/1‑141 (MQ=255)

                                                                                                                   tgATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATctacta                >  3:216401/1‑141 (MQ=255)

                                                                                                                   tgATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATctacta                >  2:332801/1‑141 (MQ=255)

                                                                                                                   tgATCCATAAATAATTAGTCATATCGAATAATGTCATATTCATGCTCCTTTTATTTGAGTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTATACAATAGCCGTCTGTTTCAAGCAATATTCTATctacga                >  2:301019/1‑139 (MQ=255)

                                                                                                                      tCCATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATCTACTAATa             >  1:749171/1‑141 (MQ=255)

                                                                                                                        cATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATCTACTAATAGa           >  3:222021/1‑141 (MQ=255)

                                                                                                                        cATAAATAATTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATCTACTAATAGa           >  2:611050/1‑141 (MQ=255)

                                                                                                                         ataaataaTTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATCTACTAATAGaa          >  2:240189/1‑141 (MQ=255)

                                                                                                                         ataaataaTTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGAATACATTTTATTATAAAGTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATCTACTAATAGaa          >  4:278400/1‑141 (MQ=255)

                                                                                                                          taaataaTTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATCTACTAATAGaaa         >  2:485285/1‑141 (MQ=255)

                                                                                                                            aataatTAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATCTACTAATAGaaaaa       <  3:183296/141‑1 (MQ=255)

                                                                                                                                 ttAGTCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATCTACTAATAGAAAAATCAtt  <  3:167712/141‑1 (MQ=255)

TTTGGAATAAAAAATGCAAGCACACCTAATATAACAAATGTAATTACTGCAGATACAAACCATTTATTTAAAACTAAGCAACAGAATATTGTTAATAAAATCATTATTAATGTTGTGATCCATAAATAATTAGGCATATCGAATAATGTCATATTCATTCTCCTTTTATTTCATTACTTTCCTTGTATACATTTTATTATAAATTTTTAAAAACTTAAACAATAGCAGTCAGTTTCAAGCAATATTCTATCTACTAATAGAAAAATCATT  >  USA300TCH1516_ALE/1419394‑1419663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`