Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 2,502,141 C→A A222D (GCC→GAC)  melR_2 → Melibiose operon regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,502,1410CA99.1% 367.0 / ‑3.3 112A222D (GCC→GAC) melR_2Melibiose operon regulatory protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (62/48);  total (63/49)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 112 total aligned reads displayed.

TGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTATCACAAT  >  USA300TCH1516_ALE/2502004‑2502278
                                                                                                                                         |                                                                                                                                         
tGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACgg                                                                                                                                        <  4:267170/141‑1 (MQ=255)
tGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACgg                                                                                                                                        >  3:282041/1‑141 (MQ=255)
       tatTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAAc                                                                                                                                 >  4:88577/1‑141 (MQ=255)
          tGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGt                                                                                                                              >  1:102694/1‑141 (MQ=255)
           gTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTc                                                                                                                             >  3:718/1‑141 (MQ=255)
           gTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTc                                                                                                                             >  3:55966/1‑141 (MQ=255)
           gTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTGTCTACAAAACATTCTATTACAACTGTTTCTGAGTTGGACGGTTTTAACAGTc                                                                                                                             >  3:23387/1‑141 (MQ=255)
            ttCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCa                                                                                                                            <  1:181593/141‑1 (MQ=255)
            ttCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCa                                                                                                                            <  1:632636/141‑1 (MQ=255)
             tCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCAt                                                                                                                           <  4:25434/141‑1 (MQ=255)
              cTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATa                                                                                                                          <  2:56998/141‑1 (MQ=255)
               tAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATAc                                                                                                                         >  1:34937/1‑141 (MQ=255)
                 aTCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACaa                                                                                                                       >  2:594131/1‑141 (MQ=255)
                 aTCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTATTAACAGTCATACaa                                                                                                                       <  4:166573/141‑1 (MQ=255)
                  tCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACaaa                                                                                                                      >  2:66923/1‑141 (MQ=255)
                   cTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAAt                                                                                                                     >  1:95892/1‑141 (MQ=255)
                   cTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAAt                                                                                                                     >  1:95947/1‑141 (MQ=255)
                    tttttGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAAtt                                                                                                                    >  2:232599/1‑141 (MQ=255)
                     ttttGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAAttt                                                                                                                   <  1:734712/141‑1 (MQ=255)
                         gTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCa                                                                                                               >  1:374604/1‑141 (MQ=255)
                           tCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCaaa                                                                                                             >  1:221212/1‑141 (MQ=255)
                            cGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAAt                                                                                                            >  1:807626/1‑141 (MQ=255)
                            acgaatTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAAt                                                                                                            <  2:358596/137‑1 (MQ=255)
                              atatTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCa                                                                                                          <  2:746922/141‑1 (MQ=255)
                                   tGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTa                                                                                                     >  3:363305/1‑141 (MQ=255)
                                    gAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAGAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTaa                                                                                                    <  2:301515/141‑1 (MQ=255)
                                    gAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTaa                                                                                                    <  1:602603/141‑1 (MQ=255)
                                     aGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAg                                                                                                   >  3:67890/1‑141 (MQ=255)
                                      gTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGa                                                                                                  >  1:526943/1‑141 (MQ=255)
                                      gTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGa                                                                                                  >  2:175952/1‑141 (MQ=255)
                                        aTGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAAt                                                                                                <  2:470481/141‑1 (MQ=255)
                                           aaTTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTAc                                                                                             >  1:572091/1‑141 (MQ=255)
                                            aTTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACt                                                                                            <  2:544941/141‑1 (MQ=255)
                                            aTTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACt                                                                                            <  2:771863/141‑1 (MQ=255)
                                            aTTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACt                                                                                            <  3:181812/141‑1 (MQ=255)
                                            aTTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACt                                                                                            <  1:279006/141‑1 (MQ=255)
                                              tttAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTg                                                                                          >  1:329493/1‑141 (MQ=255)
                                                tAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCa                                                                                        >  2:530315/1‑141 (MQ=255)
                                                    gATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCAtttt                                                                                    >  1:708873/1‑141 (MQ=255)
                                                      ttattTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAg                                                                                  >  3:243361/1‑141 (MQ=255)
                                                      ttattTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAg                                                                                  <  4:15738/141‑1 (MQ=255)
                                                       tattTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGt                                                                                 <  3:28688/141‑1 (MQ=255)
                                                       tattTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGt                                                                                 >  3:15643/1‑141 (MQ=255)
                                                       tattTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGt                                                                                 >  1:112243/1‑141 (MQ=255)
                                                         ttttACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTcc                                                                               <  3:206063/141‑1 (MQ=255)
                                                         ttttACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTcc                                                                               <  2:300375/141‑1 (MQ=255)
                                                         ttttACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTcc                                                                               <  1:484393/141‑1 (MQ=255)
                                                                  tttaataCTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGtt                                                                      >  2:143601/7‑141 (MQ=255)
                                                                    ttAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTcc                                                                    <  1:189458/141‑1 (MQ=255)
                                                                       aaCTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGtt                                                                 <  1:751819/141‑1 (MQ=255)
                                                                        aCTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTTCATTTTAGTCCTAAACAGTTCCGTTc                                                                >  4:337009/1‑141 (MQ=255)
                                                                        aCTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTc                                                                >  2:96853/1‑141 (MQ=255)
                                                                        aCTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTc                                                                >  2:630683/1‑141 (MQ=255)
                                                                         cTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCa                                                               <  1:834355/141‑1 (MQ=255)
                                                                          ttGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCAc                                                              <  3:175210/141‑1 (MQ=255)
                                                                           tGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACt                                                             >  4:21877/1‑141 (MQ=255)
                                                                           tGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACt                                                             <  1:711333/141‑1 (MQ=255)
                                                                           tGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACt                                                             <  1:855047/141‑1 (MQ=255)
                                                                                aCGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGttt                                                        >  1:315291/1‑141 (MQ=255)
                                                                                  gCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTcc                                                      <  2:12875/141‑1 (MQ=255)
                                                                                       cAATTTATTACTTTCTACAAAACCTTCTATTACTACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATGACTTGCATGTTAGTCCTAAACAGTTTCGTTCACTCGTTTCCAAGAt                                                 >  4:238511/1‑141 (MQ=255)
                                                                                       cAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGAt                                                 <  2:387356/141‑1 (MQ=255)
                                                                                         aTTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTa                                               <  4:11066/141‑1 (MQ=255)
                                                                                         aTTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTa                                               >  1:58887/1‑141 (MQ=255)
                                                                                            tattaCTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTg                                            >  3:198888/1‑141 (MQ=255)
                                                                                            tattaCTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTg                                            >  3:145789/1‑141 (MQ=255)
                                                                                            tattaCTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATATAAGAAGTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTg                                            >  1:35163/1‑141 (MQ=255)
                                                                                             attaCTTTCTACAAAACATTCTATTACAACTGGTTCTGAATTGGACGGTTTTAACAGTTATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCTGTTCACTCGTTTCCAACATTACTGa                                           >  2:557496/1‑141 (MQ=255)
                                                                                              ttaCTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGaa                                          >  1:6200/1‑141 (MQ=255)
                                                                                               taCTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAac                                         >  1:476985/1‑141 (MQ=255)
                                                                                                   ttCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAaccacc                                     <  3:337446/141‑1 (MQ=255)
                                                                                                        cAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACaaa                                >  2:306000/1‑141 (MQ=255)
                                                                                                          aaaCATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATa                              >  2:525132/1‑141 (MQ=255)
                                                                                                          aaaCATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATa                              >  2:261663/1‑141 (MQ=255)
                                                                                                           aaCATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATac                             >  4:249796/1‑141 (MQ=255)
                                                                                                           aaCATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATac                             <  3:365055/141‑1 (MQ=255)
                                                                                                           aaCATTCTATTACAACAGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCAATTTAGTTCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATac                             >  2:146753/1‑141 (MQ=255)
                                                                                                            aCATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATaca                            >  2:566152/1‑141 (MQ=255)
                                                                                                              aTTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACt                          <  2:780333/141‑1 (MQ=255)
                                                                                                               ttCTATTACAACTGTTTCTGAATTGGACGGTTTTAACTTTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACtt                         >  4:172974/1‑141 (MQ=255)
                                                                                                                tCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTc                        >  3:129680/1‑141 (MQ=255)
                                                                                                                tCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTc                        >  2:619708/1‑141 (MQ=255)
                                                                                                                 cTATTACTACTGTTTCTGAATTGGACGGTTTTAACAGTAATACAAATTGTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTCCCGTTCACTCGTTTCCAAGATTACACAACCACCACAAATACACTTcc                       <  4:119072/141‑1 (MQ=255)
                                                                                                                 cTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCCTACAAATTTTGCAAATCCATCTAAGAATTACTTGCATTCTAGTCCTCAACAGTTCAGTTCACACGGTTCCAAGAATACTCAACCACGACAAATACACTccc                       >  2:230727/1‑141 (MQ=255)
                                                                                                                  tATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCca                      >  2:343209/1‑141 (MQ=255)
                                                                                                                    ttACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCcaac                    >  2:255087/1‑141 (MQ=255)
                                                                                                                       cAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAg                 >  2:683573/1‑141 (MQ=255)
                                                                                                                       cAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTACTCCTACACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAg                 <  3:163924/141‑1 (MQ=255)
                                                                                                                         aCTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGAt               <  1:175952/141‑1 (MQ=255)
                                                                                                                         aCTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGAt               <  1:605844/141‑1 (MQ=255)
                                                                                                                         aCTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGAt               >  1:189775/1‑141 (MQ=255)
                                                                                                                           tGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATaa             <  1:756461/141‑1 (MQ=255)
                                                                                                                            gTTTCTGAATTGGACGGTTATAACAGTCATAGAAATTTTGCAGATCAATTTAACAATTACTTGCATTTTCGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGGTTACTGAGCCACCACAAATACACTACCAACTCGATacc            <  2:844355/141‑3 (MQ=255)
                                                                                                                              ttCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGt          <  1:378670/141‑1 (MQ=255)
                                                                                                                                cTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTAt        <  1:434348/141‑1 (MQ=255)
                                                                                                                                 tGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTATc       >  1:736307/1‑141 (MQ=255)
                                                                                                                                 tGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTATc       <  2:804238/141‑1 (MQ=255)
                                                                                                                                  gAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTATca      <  4:253921/141‑1 (MQ=255)
                                                                                                                                      tGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTATCACAAt  >  1:159679/1‑141 (MQ=255)
                                                                                                                                      tGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACGTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTATCACAAt  >  2:681731/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTATCACAAT  >  USA300TCH1516_ALE/2502004‑2502278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: