BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	423,247	A→G	V112V (GTA→GTG)	USA300HOU_RS01970 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	423,247	0	A	G	95.7%	386.8 / 5.0	117	V112V (GTA→GTG)	USA300HOU_RS01970	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (4/1); new base G (65/46); total (69/48)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

Only 100 of 117 total aligned reads displayed.

AGTGTTTTTAATAAAGAAGGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTACCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTATGAATAAAATTAA  >  USA300TCH1516_ALE/423110‑423383

aGTGTTTTTAATAAAGAAGGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCa                                                                                                                                       >  4:152208/1‑141 (MQ=255)

   gtTTTTAATAAAGAAGGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCaaag                                                                                                                                    <  3:11657/141‑1 (MQ=255)

   gagtttAATAAAGAAGGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCaaag                                                                                                                                    <  1:39550/138‑1 (MQ=255)

     ttttAATAAAGAAGGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCaaagaa                                                                                                                                  >  1:540380/1‑141 (MQ=255)

       ttAATAAAGAAGGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCaaagaaag                                                                                                                                >  1:233415/1‑141 (MQ=255)

       ttAATAAAGAAGGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCaaagaaag                                                                                                                                >  1:270779/1‑141 (MQ=255)

       ttAATAAAGAAGGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCaaagaaag                                                                                                                                >  1:656436/1‑141 (MQ=255)

        taataaAGAAGGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGc                                                                                                                               <  1:121867/141‑1 (MQ=255)

             aagaagGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAg                                                                                                                          <  1:475911/141‑1 (MQ=255)

              agaagGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGa                                                                                                                         >  4:353857/1‑141 (MQ=255)

               gaagGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGAc                                                                                                                        >  1:414011/1‑141 (MQ=255)

               gaagAGAAAGCGACGCAATTAATGTATACTGATAACTTAGAAGTTAAACCGCATAAAAACTAAAAAATTAAAAATAAAGGTAAAAATGTAAAAAAATTTGAAGAAGTTGAAAAGGAAGAAGAGAGAAAGAAAGCATTAGAc                                                                                                                        <  4:55157/141‑1 (MQ=255)

                     aaaGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTg                                                                                                                  >  4:107347/1‑141 (MQ=255)

                      aaGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTTGACAAACTGa                                                                                                                 >  2:769458/1‑141 (MQ=255)

                      aaGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTTGACAAACTGa                                                                                                                 >  2:769516/1‑141 (MQ=255)

                      aaGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGa                                                                                                                 >  1:815502/1‑141 (MQ=255)

                      aaGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGa                                                                                                                 >  1:815543/1‑141 (MQ=255)

                      aaGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGa                                                                                                                 <  1:43726/141‑1 (MQ=255)

                           aCGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGa                                                                                                            >  2:384083/1‑141 (MQ=255)

                              cAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACAAAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAATAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGataa                                                                                                         <  2:820656/141‑1 (MQ=255)

                               aaTTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGataat                                                                                                        <  1:736021/141‑1 (MQ=255)

                               aaTTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGataat                                                                                                        <  2:380155/141‑1 (MQ=255)

                                   aaTGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCa                                                                                                    <  3:233713/141‑1 (MQ=255)

                                    aTGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCAt                                                                                                   >  1:703410/1‑141 (MQ=255)

                                    aTGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCAt                                                                                                   <  1:195601/141‑1 (MQ=255)

                                      gTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGc                                                                                                 >  1:606748/1‑141 (MQ=255)

                                       tataCTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCt                                                                                                >  3:230684/1‑141 (MQ=255)

                                        ataCTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCtt                                                                                               >  3:101454/1‑141 (MQ=255)

                                        ataCTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCtt                                                                                               >  3:365752/1‑141 (MQ=255)

                                         taCTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACCGAGTCGATAATAGCATGCTta                                                                                              <  3:315053/141‑1 (MQ=255)

                                         taCTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACCGAGTCGATAATAGCATGCTta                                                                                              <  3:315045/141‑1 (MQ=255)

                                             gCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTACCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATAtt                                                                                          >  4:302008/1‑141 (MQ=255)

                                              cTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATAATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTACCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTa                                                                                         >  3:268645/1‑141 (MQ=255)

                                                aCATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACa                                                                                       <  1:754537/141‑1 (MQ=255)

                                                 cATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACAt                                                                                      >  3:302625/1‑141 (MQ=255)

                                                     tGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTACCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGtt                                                                                  >  2:837185/1‑141 (MQ=255)

                                                       aTGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCa                                                                                <  2:435378/141‑1 (MQ=255)

                                                       aTGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCa                                                                                <  2:494491/141‑1 (MQ=255)

                                                       aTGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCa                                                                                <  1:274596/141‑1 (MQ=255)

                                                       aTGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCa                                                                                <  1:298366/141‑1 (MQ=255)

                                                        tGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCAt                                                                               <  3:304258/141‑1 (MQ=255)

                                                         gTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCAtt                                                                              >  4:61282/1‑141 (MQ=255)

                                                         gTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCAtt                                                                              >  1:852976/1‑141 (MQ=255)

                                                           tAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTta                                                                            <  2:564799/141‑1 (MQ=255)

                                                           tAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTta                                                                            >  1:197306/1‑141 (MQ=255)

                                                             aaCCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTtata                                                                          >  1:564556/1‑141 (MQ=255)

                                                              aCCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATaa                                                                         >  4:154710/1‑141 (MQ=255)

                                                               ccGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATaaa                                                                        <  1:157670/141‑1 (MQ=255)

                                                                cGCATAGATACCTGAAAATTACACATAAAGGACATCATGTAGAACCTTTTGAAGAAGCAGAAAAGGAAGAAGTGCCAAAGACAGCATTAGACAAACTGAGTCGATCATAGCATGCTTATATTACATGGTTCATTTATaaaa                                                                       >  2:644159/1‑141 (MQ=255)

                                                                 gCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAg                                                                      >  2:156225/1‑141 (MQ=255)

                                                                  cATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAgg                                                                     >  2:241665/1‑141 (MQ=255)

                                                                   aTAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGa                                                                    >  4:314291/1‑141 (MQ=255)

                                                                             gAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTAGATTACATGGTTCATTTATAAAAGGAGTacgaacga                                                          <  1:754889/141‑1 (MQ=255)

                                                                               aaaTTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGaaa                                                        <  4:218802/141‑1 (MQ=255)

                                                                               aaaTTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGaaa                                                        <  2:562479/141‑1 (MQ=255)

                                                                               aaaTTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGaaa                                                        <  2:562403/141‑1 (MQ=255)

                                                                               aaaTTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTACCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGaaa                                                        <  2:279714/141‑1 (MQ=255)

                                                                                  ttACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGATGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTa                                                     >  4:189421/1‑141 (MQ=255)

                                                                                    acacATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAAc                                                   >  1:234553/1‑141 (MQ=255)

                                                                                    acacATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTGGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAAc                                                   >  1:459828/1‑141 (MQ=255)

                                                                                       caTAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCa                                                >  2:390337/1‑141 (MQ=255)

                                                                                       caTAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCa                                                >  2:348965/1‑141 (MQ=255)

                                                                                         aaaaGGTCATCATGAAAAACCTTTTAAAGAAGTTGAAAAGGAAGAAGTGCC‑AAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGa                                             <  3:215606/140‑1 (MQ=255)

                                                                                          aaaGGTCATCATGTAGAAACTTTTTTTTTTTTTTTAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATCTTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGa                                             >  2:308762/1‑141 (MQ=255)

                                                                                          aaaGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGa                                             >  1:125074/1‑141 (MQ=255)

                                                                                             ggTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGa                                          >  1:712797/1‑141 (MQ=255)

                                                                                              gTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGAt                                         <  2:841784/141‑1 (MQ=255)

                                                                                               tcatcaTGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGAtt                                        >  1:56526/1‑141 (MQ=255)

                                                                                                 atcatGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTaa                                      >  2:742440/1‑141 (MQ=255)

                                                                                                 atcatGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTaa                                      >  4:250542/1‑141 (MQ=255)

                                                                                                 atcatGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTaa                                      >  3:24842/1‑141 (MQ=255)

                                                                                                   catGTAGAAACTTTTGAAGAAGTTGAAGAGGAAGAAGTGCCAAAGAAAGCATTAGAGAAACTGATGCGAGAAGAGCATGCATAGATGACATGGTTCATTTATAAAAGTAGTACGAACGAAGGTAACTCATGACGAGTAAtt                                    >  3:312845/1‑141 (MQ=255)

                                                                                                      gTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTaa                                 >  1:37311/1‑141 (MQ=255)

                                                                                                       tAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTaaa                                >  1:457302/1‑141 (MQ=255)

                                                                                                        aGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTaaaa                               >  1:4321/1‑141 (MQ=255)

                                                                                                             cTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATAtt                          >  2:289388/1‑141 (MQ=255)

                                                                                                             cTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATAtt                          <  2:287243/141‑1 (MQ=255)

                                                                                                             cTTTTGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATAtt                          >  1:495584/1‑141 (MQ=255)

                                                                                                               tttGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTg                        <  1:776821/141‑1 (MQ=255)

                                                                                                               tttGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTg                        >  3:360024/1‑141 (MQ=255)

                                                                                                                ttGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGt                       >  1:728176/1‑141 (MQ=255)

                                                                                                                ttGAAGAAGTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGt                       <  2:5490/141‑1 (MQ=255)

                                                                                                                   aagaagTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGtaat                    >  4:246288/1‑141 (MQ=255)

                                                                                                                    agaagTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGtaata                   >  1:196198/1‑141 (MQ=255)

                                                                                                                     gaagTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGtaataa                  <  1:602945/141‑1 (MQ=255)

                                                                                                                        gTTGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTa               <  4:124504/141‑1 (MQ=255)

                                                                                                                         ttGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTAt              <  1:376367/141‑1 (MQ=255)

                                                                                                                         ttGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTAt              >  1:94085/1‑141 (MQ=255)

                                                                                                                         ttGAAAAGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTAt              >  3:242084/1‑141 (MQ=255)

                                                                                                                          tGAAAAGGAAGAAGATCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTATg             <  2:391573/141‑1 (MQ=255)

                                                                                                                            aaaaGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTATGaa           >  4:114711/1‑141 (MQ=255)

                                                                                                                             aaaGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTATGAAt          <  3:196018/141‑1 (MQ=255)

                                                                                                                               aGGAAGAAGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTATGAATaa        <  4:101454/141‑1 (MQ=255)

                                                                                                                                 gaagaaGTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTATGAATaaaa      >  1:419261/1‑141 (MQ=255)

                                                                                                                                  aagaagTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACAATTAATTTAAAAATATTTGTAATAATTATGAATAAAAt     <  2:630940/141‑1 (MQ=255)

                                                                                                                                    gaagTGCCAAAGAAAGCCTTAGACAAACTGAGACGATAATAGCATGCGTATATTACATGGATCATTTATAAAAGGAGTACGAACGAAAGTAACACAAGAAGAATAAATTAAAAATAAACATAATAAATATGAAAAAAAATa   >  2:831748/1‑141 (MQ=255)

                                                                                                                                    gaagTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTATGAATAAAATTa   >  2:697145/1‑141 (MQ=255)

                                                                                                                                     aagTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTATGAATAAAATTaa  >  2:624397/1‑141 (MQ=255)

                                                                                                                                     aagTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTATGAATAAAATTaa  <  1:266424/141‑1 (MQ=255)

                                                                                                                                     aagTGCCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTATGAATAAAATTaa  >  1:238235/1‑141 (MQ=255)

AGTGTTTTTAATAAAGAAGGGAAAGAGACGCAATTAATGTATACTGCTACATTTGATGTTAAACCGCATAGATACTTGAAAATTACACATAAAGGTCATCATGTAGAAACTTTTGAAGAAGTTGAAAAGGAAGAAGTACCAAAGAAAGCATTAGACAAACTGAGTCGATAATAGCATGCTTATATTACATGGTTCATTTATAAAAGGAGTACGAACGAAAGTAACGCATGACGATTAATTTAAAAATATTTGTAATAATTATGAATAAAATTAA  >  USA300TCH1516_ALE/423110‑423383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`