Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 941,138 G→A 7.9% Q298* (CAG→TAG)  ptlE ← Neopentalenolactone D synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE941,1380GA 7.9% 182.8 / 4.8 64Q298* (CAG→TAG) ptlENeopentalenolactone D synthase
Reads supporting (aligned to +/- strand):  ref base G (30/28);  new base A (3/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

AGATTTGGTATCCATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGTTCGTGAGGCATTGATCCTTT  >  USA300TCH1516_ALE/940999‑941276
                                                                                                                                           |                                                                                                                                          
aGATTTGGTATCCATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGt                                                                                                                                           <  1:259897/141‑1 (MQ=255)
  aTTTGGTATCCATTCTAATTTTTTTTATTTAACAGGGTAATAACATTATTTGATAGGTGCATCGTAGTTTTGTATTAATTGCATAATAAGTGGGTGTGTCATAAAATCTTGTTCAAGACCAGTTGCAAGGATAATCTAttt                                                                                                                                         >  2:256069/1‑140 (MQ=255)
   tttGGTATCCATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGa                                                                                                                                        <  2:70612/141‑1 (MQ=255)
    ttGGTATCCATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGat                                                                                                                                       <  2:216051/141‑1 (MQ=255)
      tttATTCATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATATGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGatat                                                                                                                                     >  2:302354/3‑141 (MQ=255)
      ggTATCCATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATACTCTGTTGatat                                                                                                                                     >  1:396322/1‑141 (MQ=255)
      ggTATCCATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGatat                                                                                                                                     >  2:433224/1‑141 (MQ=255)
       gTATCCATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATTTg                                                                                                                                    >  1:325010/1‑141 (MQ=255)
       gTATCCATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATg                                                                                                                                    >  1:88672/1‑141 (MQ=255)
        tATCCATTCTAATGTTTCGGATATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGCGACATAAACGCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATgg                                                                                                                                   <  2:19925/141‑1 (MQ=255)
         aTCCATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGa                                                                                                                                  <  2:249213/141‑1 (MQ=255)
           ccATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAAc                                                                                                                                <  1:428524/141‑1 (MQ=255)
             aTTCTAATTTTTCTGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAg                                                                                                                              <  2:224635/141‑1 (MQ=255)
               tCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGTTATGGAACAGaa                                                                                                                            >  1:473583/1‑141 (MQ=255)
                   aTTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCAt                                                                                                                        <  1:423673/141‑1 (MQ=255)
                   aTTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAAAAGAATCAt                                                                                                                        >  1:42196/1‑141 (MQ=255)
                      tttCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTATTGATATGGAACAGAATCAtttt                                                                                                                     >  2:461855/1‑141 (MQ=255)
                      tttCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTATTGATATGGAACAGAATCAtttt                                                                                                                     >  2:390381/1‑141 (MQ=255)
                            aaaTAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAgtgt                                                                                                               <  2:537125/141‑1 (MQ=255)
                            aaaTAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAgtgt                                                                                                               <  2:559832/141‑1 (MQ=255)
                               aaaCAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTaa                                                                                                            <  1:49091/140‑1 (MQ=255)
                                aaCAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAAGAGAATCATTTTCAGTGTTAAt                                                                                                           >  1:27626/1‑141 (MQ=255)
                                aaCAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAAt                                                                                                           >  1:359200/1‑141 (MQ=255)
                                      gTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTAcacc                                                                                                     <  2:219564/141‑1 (MQ=255)
                                          ttAAATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTAcaccacca                                                                                                 <  1:187485/141‑1 (MQ=255)
                                          atACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTGCAGTGTTAATTAcaccacca                                                                                                 <  1:394309/140‑1 (MQ=255)
                                              aTTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTaa                                                                                             >  2:13744/1‑141 (MQ=255)
                                                   ttGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATGTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGa                                                                                        <  2:439272/141‑1 (MQ=255)
                                                   ttGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGa                                                                                        <  2:159636/141‑1 (MQ=255)
                                                   ttGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACCAGTTTGa                                                                                        >  1:490028/1‑141 (MQ=255)
                                                       tAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGa                                                                                    >  2:284325/1‑141 (MQ=255)
                                                       tAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGa                                                                                    >  2:425053/1‑141 (MQ=255)
                                                       tAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTCGAGTGa                                                                                    >  2:553303/1‑141 (MQ=255)
                                                         ggTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATa                                                                                  <  2:88672/141‑1 (MQ=255)
                                                           tGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGGTAgg                                                                                >  1:127954/1‑141 (MQ=255)
                                                              atcatAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTATTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGgtgt                                                                             <  1:461855/141‑1 (MQ=255)
                                                              atcatAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTATTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGgtgt                                                                             <  1:390381/141‑1 (MQ=255)
                                                                    aTTGTGTGTTAATTGCCTACTAAGGTGGTGGGTCATAAAATCTTGTTCCAGACCAGTTGCAACCATAATCTGTTGATATGGAACATAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATg                                                                       <  1:207987/141‑1 (MQ=255)
                                                                      tttGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTATTATGca                                                                     >  2:32759/1‑141 (MQ=255)
                                                                         ttATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAACACCAATTGCAACCATAATCTGTTGATATGGAACAGAAGCATTTTCAGAGTTAATTACACCACCACTAATTGGAGTGATAGGTGTTTTATGCACAt                                                                  <  2:366525/140‑1 (MQ=255)
                                                                         gTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACAt                                                                  >  2:397213/1‑141 (MQ=255)
                                                                                      aataaGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCAtt                                                     >  1:99279/1‑141 (MQ=255)
                                                                                           gTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTaa                                                >  1:58747/1‑141 (MQ=255)
                                                                                           gTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTaa                                                >  1:368081/1‑141 (MQ=255)
                                                                                           gTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAAAATCATTTTCAGTGTTAATTACACCACCACTAATTTAAGTGATAGGTGTTTTATGCACATTTATACGACCATTCTTaa                                                >  2:105392/1‑141 (MQ=255)
                                                                                              gTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAatat                                             <  2:495506/141‑1 (MQ=255)
                                                                                              gTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAatat                                             <  2:538376/141‑1 (MQ=255)
                                                                                                   gACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGtttt                                        >  1:209396/1‑141 (MQ=255)
                                                                                                     caaaaaaTCTTGTGCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGtttttt                                      <  2:325010/138‑1 (MQ=255)
                                                                                                     cATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGtttttt                                      >  2:427497/1‑141 (MQ=255)
                                                                                                          aaTCTTTTTCACAACCAGTTGCAACCATAATCTGTTGATATGGAACATAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGc                                 <  2:151466/141‑1 (MQ=255)
                                                                                                           aTCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCg                                <  1:302354/141‑1 (MQ=255)
                                                                                                               tGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAg                            >  1:516417/1‑141 (MQ=255)
                                                                                                                     aaaCCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGt                      >  2:287431/1‑141 (MQ=255)
                                                                                                                     aaaCCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGt                      >  1:513947/1‑141 (MQ=255)
                                                                                                                      aaCCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGtt                     >  2:457962/1‑141 (MQ=255)
                                                                                                                           gTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGTTCGTgg                >  2:558720/1‑140 (MQ=255)
                                                                                                                              gCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGTTCGTGAGGc             <  1:178329/141‑1 (MQ=255)
                                                                                                                               cAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCTTTGGTAATATGTTTTTTAAGGCGTAAGTACAGTTCGTGAGGCa            <  1:530154/141‑1 (MQ=255)
                                                                                                                               cAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGTTGCTCAGGCa            >  2:452175/1‑141 (MQ=255)
                                                                                                                               cAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGTTCGTGAGGCa            <  2:209396/141‑1 (MQ=255)
                                                                                                                                 actacaaTCTCTTCATATGGAATATAAGCATTTTCAGTGTTAATTACACCAACACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGTTCGTGAGGCAtt          <  1:539862/136‑1 (MQ=255)
                                                                                                                                 aCCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCCCATTTATACGACCATTTTTAATATGTTTTTTAAGCCGTAAGTACAGTTCGTGAGGCAtt          <  1:410588/141‑1 (MQ=255)
                                                                                                                                         cTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGTTCGTGAGGCATTGATCCttt  >  1:398237/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AGATTTGGTATCCATTCTAATTTTTCGGAAATAACAGGGTAATTACATTCGTTGATAGGTGCATCATAATTTTGTATTAATTGCTTAATAAGTGGTTGTGACATAAAATCTTGTTCAAAACCAGTTGCAACCATAATCTGTTGATATGGAACAGAATCATTTTCAGTGTTAATTACACCACCACTAATTTGAGTGATAGGTGTTTTATGCACATTTATACGACCATTTTTAATATGTTTTTTAAGGCGTAAGTACAGTTCGTGAGGCATTGATCCTTT  >  USA300TCH1516_ALE/940999‑941276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑