| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,744,454 | C→T | 100% | G73D (GGC→GAC) | apt ← | Adenine phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,744,454 | 0 | C | T | 100.0% | 124.7 / ‑6.5 | 45 | G73D (GGC→GAC) | apt | Adenine phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (26/18); total (26/19) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAT > USA300TCH1516_ALE/1744318‑1744586 | atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATACTAGGTCTTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAAt > 2:2708/1‑141 (MQ=255) tcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATc < 2:75509/141‑1 (MQ=255) ttAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTg < 1:267439/141‑1 (MQ=255) ttAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTg < 1:267534/141‑1 (MQ=255) tAACACACGTTGACCTGATTTTGGCGCAGCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGa > 2:542743/1‑141 (MQ=255) acacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAAt < 2:102812/141‑1 (MQ=255) cacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACAAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATa > 1:236427/1‑141 (MQ=255) acacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATaa > 1:544871/1‑141 (MQ=255) cTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTGTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGAc > 1:494493/1‑141 (MQ=255) gCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATa > 1:60854/1‑141 (MQ=255) aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGTATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTCCAGGACAGCCAATGATaaa > 1:74678/1‑141 (MQ=255) aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCCTTGAATAAGCTACAGGACAGCCAATGATaaa > 1:74677/1‑141 (MQ=255) aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCATTCTTTCTTAACAGATATAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATaaa > 2:170587/1‑141 (MQ=255) tgtgCATTGTTAAAACATTTGTACCAAATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGCTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCACAGAGAAAGCCAc > 1:536630/1‑141 (MQ=255) aaCATTTCTGCCATATTCTAGGTCATACTCATAACGAATGGCTGCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACTGCCAATGATAAAGCCACGCGCTTCAGGTca < 2:346748/141‑2 (MQ=255) aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt < 1:515141/141‑1 (MQ=255) cATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAAGGTGAAAGCCACGCGCTTCAGGTCCTa < 2:541850/141‑1 (MQ=255) aTTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTAc < 2:129930/141‑1 (MQ=255) aTTGGTACCAGATTCTAGGGCATACTCATAACGAATGACTTCACGATGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCTATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTAc < 2:87217/141‑1 (MQ=255) ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa > 2:492150/1‑141 (MQ=255) aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGat > 2:331801/1‑141 (MQ=255) ccATATTCTAGGTCATACTCATAACGAATGACTACACGAGCTAATTTCCTTTCTATTCTAACAGGTGCAAAGTCAATCCCCAGTGAATAATCTACAGGACAGCCAATGATAAAGCCACTCGCATCAGGTACTACAGCGaga > 2:370295/1‑139 (MQ=255) aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTATAGCAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAAGGGTAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct > 1:324904/1‑141 (MQ=255) ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc > 2:43303/1‑141 (MQ=255) cATACTCATAACGAATGACTTCACGAGGTAATTTTTTTTTTTTTTTTTTTTCTGCAAAGTAAGGCCCCATTGAATAAGCAAAATGACAGCCAATGATAAAGTCACGCGCTTCAGGTCCTTCACGGATATCACCATCTCTGt > 2:229614/1‑141 (MQ=255) aTAACGAATGACTTCACGAGGTACTTTCCCTTCTTTTCTAACAGCTGCAAAGTCAATCACCATTGAATAAGCTACAGGACAGCCAATGCTAAAGCCACGCGCTTCAGGTCCTACAACGACATAACCATCTCTGTCTTTTGc > 2:150150/1‑141 (MQ=255) aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGTTATCAACATCTCTGTCTTTTGc > 1:143803/1‑141 (MQ=255) gAATGACTTCACGAGGTAATTTCCCTTTTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCtttttcatctt > 1:496494/1‑134 (MQ=255) gAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTATGTCTTTTGCGTAtt > 1:496490/1‑141 (MQ=255) gAATGACATCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAtt > 1:353033/1‑141 (MQ=255) aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt > 1:7280/1‑141 (MQ=255) gACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTAc > 1:40378/1‑141 (MQ=255) aCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa > 1:509691/1‑141 (MQ=255) aCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAACCCATTTTTAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa < 1:494586/141‑1 (MQ=255) cGAGGTAATTTCCCTTCTTTTCTAACCGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTa < 2:431246/141‑1 (MQ=255) gTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg < 1:191302/141‑1 (MQ=255) gTAATTTCCCTTCTTTTCCAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg < 2:74014/141‑1 (MQ=255) tAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt < 1:439450/141‑1 (MQ=255) tAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt < 2:544871/141‑1 (MQ=255) ttcttTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGcc < 1:170587/141‑1 (MQ=255) tttCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGACATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCAAAGCCatat > 2:29954/1‑141 (MQ=255) tAACAGGTCCAAAGTCAATCCCCATGGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCtt < 1:125931/141‑1 (MQ=255) aCAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGCCAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGCCATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCa < 1:518251/141‑1 (MQ=255) cAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc > 2:337933/1‑141 (MQ=255) gTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCACTGGTAGAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTCATCTGTTGCATAGCCATATGCTTCACCAt < 1:43303/141‑1 (MQ=255) | ATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAT > USA300TCH1516_ALE/1744318‑1744586 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |