| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 479,553 | G→A | 100% | A194T (GCA→ACA) | USA300HOU_RS02280 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 479,553 | 0 | G | A | 100.0% | 183.5 / ‑6.5 | 59 | A194T (GCA→ACA) | USA300HOU_RS02280 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (25/33); total (25/34) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTA > USA300TCH1516_ALE/479416‑479686 | gTGATGCAATAAAAGATGAACGAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTATATCGAGTGGGACAATGCCGAAGCGTGAGCAAAGTTTTTACcatacat > 1:291101/1‑141 (MQ=255) gaggccataataGATGAACCAGGTGGCCCACTGATCCAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGTTCAATTTTTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATgg < 2:277277/135‑1 (MQ=255) aTGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGt < 2:484778/141‑1 (MQ=255) aaataAATGAACAGTGTGAGCCCCTTAGCGAACAAGTGAACCGTCAAATGAGTAAATGGACGAAACTTTATATCGATCAATTTCGATCGAGTTAGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAtt < 2:482940/140‑1 (MQ=255) gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAGTGATTAAATGGACGAAACTTGATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgcg < 1:85435/141‑1 (MQ=255) aaTGAACAAGGCGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATCAAATGGACGAAACTTTATATCGATCAATTTCTAGCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgcg < 1:215770/140‑1 (MQ=255) aTGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGGGCAAAGTTTTTACCATACATGGTTGCATTTAgcgc < 1:444183/141‑1 (MQ=255) tGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCa > 2:421072/1‑141 (MQ=255) tGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCa < 1:421072/141‑1 (MQ=255) cAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACAt < 2:103715/141‑1 (MQ=255) ggggAGCCACTTAGCGAACCAATGGATCGTCAAATGATTAAATGGACGAAACTTTATATAGATCAAGTTCTATCGAGTTGGACAGTGCAGACGCGTGAGCAAGGTTTTTACCATACATGGTTGCATTGAGCGCAACATGAc > 2:145842/4‑141 (MQ=255) ggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTCCCATACATGGTTGCATTTAGCGCAACATGAc > 1:83275/1‑141 (MQ=255) gTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGAcc > 2:409061/1‑141 (MQ=255) tGAGCCACTCAGCGAACAAGTGAATCGTCAAACGCATTAATGGAAGAATCTTCATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACCTGGTTGCATTTAGCGCAACATGACCa < 2:394086/141‑1 (MQ=255) gAGCCATTTAGCGAACAAGTTAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCAt < 1:351744/141‑1 (MQ=255) gAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCAt > 1:514320/1‑141 (MQ=255) gAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCAt < 1:97624/141‑1 (MQ=255) aCTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCACTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCCTACATGCCTGCATTTAGCGCAACATGACCAAAGctt > 2:205800/1‑141 (MQ=255) aCTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTGTTTACCATACATGGTTGCATTTAGCGCAACATGACCAAAGttt > 2:205882/1‑141 (MQ=255) cTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGtttt > 2:16773/1‑141 (MQ=255) aaCACGTGAATCGTCAAATGATTAAATGGACGAAACTTTATAACGATCAATTTCTATCGGGTTGGCCAATGCCGAAGCGGGAGCGAAGGTTTTACCATACATGGTTGCGTTTAGCGCAACATGACCCGAGACTTACTAAAg > 2:69894/1‑141 (MQ=255) aaCAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGATCATAGTTTTACTAAAg > 1:200441/1‑141 (MQ=255) aaCAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACAGGACCATAGTTTTACTAAAg < 1:335579/141‑1 (MQ=255) aCAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGc > 1:160118/1‑141 (MQ=255) aCAAGTGAAGCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGc < 2:102781/141‑1 (MQ=255) gcaTCGTCAAATGATTAAATGGACGAAACTTTACATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcg < 2:115426/139‑1 (MQ=255) gagacGTTAAATGATTAGATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCACAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcg < 2:251683/137‑1 (MQ=255) aaTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGATGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATAACCATAGTTTTACTCAAGCACAgcgc > 2:343347/1‑141 (MQ=255) gTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAGGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTACCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAg > 1:14262/1‑141 (MQ=255) cAAATGATTAAATGGACGAAACTTTATATCGAGCAATTTCTATCGAGTTGGAAAATGCCGAAGCGTGAGGAAAGTTTTTACCATACATGGTTGCATTTAGCGTAACATGAACATAGTTTTACTAAAGAACAGCGCAAAGTg > 2:362657/1‑141 (MQ=255) aTGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGCGAtt > 1:138871/1‑141 (MQ=255) tAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAgg < 1:163996/141‑1 (MQ=255) tAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAgg < 2:448479/141‑1 (MQ=255) tAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAgg < 2:52376/141‑1 (MQ=255) tAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATCCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAGGTGATTAAAgg > 1:108022/1‑141 (MQ=255) tAAAGGGACGAAACTTGATATCGATCAATTGCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCAGAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAgg < 1:231579/141‑1 (MQ=255) ttATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATcc > 2:75252/1‑141 (MQ=255) tataTCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCt < 2:219453/141‑1 (MQ=255) tatCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGa < 2:216681/141‑1 (MQ=255) aTCAATTTCTATCGAGTTGGACAATGCCGAAGCTTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCCTAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTGCCCAATGATCCTGAAATGa < 2:313810/141‑1 (MQ=255) tCAGTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGAc < 2:63727/141‑1 (MQ=255) tttCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCCTGGGTGATTGCGTGTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATa < 1:243240/141‑1 (MQ=255) tttCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATa < 2:555722/141‑1 (MQ=255) tttCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATa > 1:25292/1‑141 (MQ=255) tttCGATCGAGTTGGACAATGCCGAAGCGTGCGCAACGTTTATACCATACATGGTTGAATCAAGCGCAACTTGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATGAAAGGCTGACCCATTGATCCTGAAATGACGATa < 2:555452/141‑1 (MQ=255) tCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCTTACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATaga < 2:287613/141‑1 (MQ=255) cGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAg < 1:435206/141‑1 (MQ=255) gAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAAAGTCAGt > 1:376971/1‑141 (MQ=255) aGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGGGTCAGtt > 2:17282/1‑140 (MQ=255) aGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTa > 1:470110/1‑141 (MQ=255) aGTTGGACAATGCCGAAGCGGGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTa < 2:125139/141‑1 (MQ=255) gACAATCGCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAAc < 2:72040/141‑1 (MQ=255) ccGAAGCGTGAGCAAAGTTTTTACCATACCTGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAtttt < 2:410271/141‑1 (MQ=255) agtaaaGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATc < 1:371353/138‑1 (MQ=255) cAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGGACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCA‑TTTTCAATAGATCAgg > 1:338330/1‑141 (MQ=255) cAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGGATTAACTCATTTTTCAATAGATCAg > 1:202744/1‑141 (MQ=255) aaGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCCTTTTTCAATAGATCCGGa > 1:245734/1‑141 (MQ=255) ttttttCCCATACATGGTTGCATTTAGCGCAACATGACCATAGCTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAg < 2:520822/140‑1 (MQ=255) gTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGCTCAGGAAg > 1:354234/1‑141 (MQ=255) ttACCATAAATGGTTGCATTTAGCGAAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTa < 2:121774/141‑1 (MQ=255) | GTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTA > USA300TCH1516_ALE/479416‑479686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |