Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 2,279,705 G→A A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,279,7050GA97.1% 315.8 / NA 106A943V (GCA→GTA) ebh_3Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (56/45);  minor base C (0/3);  total (57/49)
Fisher's exact test for biased strand distribution p-value = 9.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

TTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGTTGCAT  >  USA300TCH1516_ALE/2279561‑2279846
                                                                                                                                                |                                                                                                                                               
ttATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTGGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtt                                                                                                                                              >  1:137556/1‑147 (MQ=255)
ttATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtt                                                                                                                                              >  1:347058/1‑147 (MQ=255)
ttATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg                                                                                                                                              >  1:666118/1‑148 (MQ=255)
   tCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCTTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAACTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTCGCTGATGTTACtgttg                                                                                                                                           <  2:637524/148‑1 (MQ=255)
   tCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttg                                                                                                                                           >  2:810497/1‑148 (MQ=255)
     aattGTGCCGTTCTTAGTTTATTCTAGTTCTGGTGTACTAGTTACATCGTTATGCATTGTCGGGCTGTTTGTTGGTGCATCTACACTAGAATTGTTATTCGCTTGAGGTTTATGATTTGCCTCATGTGTTGCTGATATTTCtgttggt                                                                                                                                         >  2:282650/3‑146 (MQ=255)
     aGTTGTGCCGTTATTAGTTGAATCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTgttgtt                                                                                                                                         >  1:520689/1‑148 (MQ=255)
       ttGTGGCGTTATGAGTTGTTTCTGCTTCTGGTTTACTAGTTATTTCGTTGTTCTTTGTCGTACGGCTACCTTCTTCATCTACACTAGAATTGTTAGTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc                                                                                                                                       <  2:64150/148‑1 (MQ=255)
                 attaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGcc                                                                                                                             >  2:470187/1‑148 (MQ=255)
                    aGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTGTTAGCTTGCGGTTTATCATTTGCATCATCGGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGct                                                                                                                          >  1:906272/1‑147 (MQ=255)
                    aGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCGTTTGCATCATCAGTTGCTGATGTTACTGTTTTTTCACCTGTTGCCGCa                                                                                                                          >  1:228273/1‑148 (MQ=255)
                    aGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCTCCTGTTGCCGct                                                                                                                          >  2:929806/1‑147 (MQ=255)
                    aGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCa                                                                                                                          >  1:784296/1‑148 (MQ=255)
                    aGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCa                                                                                                                          <  2:398560/148‑1 (MQ=255)
                      tttaTTCTACTTCTGTTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                                        <  2:780187/145‑1 (MQ=255)
                      ttGGTTCTACTTCTGTTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                                        <  2:17870/148‑1 (MQ=255)
                       tGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTTTTGCCGCATcc                                                                                                                       >  2:12532/1‑147 (MQ=255)
                         aTTATACTTCTGGTTTACTAGTTAGATCGTTATCCATTGTCTGACTGTTTGTTGATGCATCTACACTAGCATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACt                                                                                                                     <  1:341421/148‑1 (MQ=255)
                            cTACTTCTGGTTTACTCGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGTTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtatt                                                                                                                  <  1:152307/148‑1 (MQ=255)
                                ttCTGGTTTACTAGTTACATTGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAttt                                                                                                              <  1:867175/148‑1 (MQ=255)
                                 tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg                                                                                                             >  1:384157/1‑148 (MQ=255)
                                   tGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt                                                                                                           <  1:835382/148‑1 (MQ=255)
                                     gcttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGCATTGTTATTAGCTTGCGGTTTCTCATTTGCATCATCAGTTGCGGATGTTACCGTTGTTTCACCTGTTGCCGCATCACTATCATCTGgtgt                                                                                                       >  2:477914/3‑148 (MQ=255)
                                     gTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgtgt                                                                                                       >  1:456642/1‑148 (MQ=255)
                                       ttACTAGTTACATTGTTATCTATTTTTAGACTGTTTGTTGATGCATCTACACTAGAATTGATATTAGCTTGCGGTTTATCATTTGCATAATCAGTTGCTGATGTTACTGTTGTTTCACCTTTTGCCGCATCACTATTATTTGGtgttg                                                                                                     <  1:70148/148‑1 (MQ=255)
                                       ttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgttg                                                                                                     <  1:594204/148‑1 (MQ=255)
                                        tACTAGTTCCATCGTTATCCATTGTCGGGCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgttgt                                                                                                    <  2:672960/148‑1 (MQ=255)
                                        tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgttgt                                                                                                    <  1:712463/148‑1 (MQ=255)
                                        tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgttgt                                                                                                    <  1:944325/148‑1 (MQ=255)
                                        tACTAGTGACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATGAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTCCACCTGTTGCCGCATCACTATTATTTGGtgttgt                                                                                                    <  1:944259/148‑1 (MQ=255)
                                           tAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCgg                                                                                                 >  2:331805/1‑148 (MQ=255)
                                           tAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGACGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCgg                                                                                                 >  1:879542/1‑148 (MQ=255)
                                            tgTTACAGCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGga                                                                                                <  1:108065/147‑1 (MQ=255)
                                             gTTACCTCGTTATCCATTGTCTGACTGTTTGTTGATCCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgag                                                                                               <  2:958454/148‑1 (MQ=255)
                                               tACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCGCGATTATTTGGTGTTGTCGGAGaa                                                                                             >  1:788368/1‑148 (MQ=255)
                                               tACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGaa                                                                                             >  2:491852/1‑148 (MQ=255)
                                               tACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGaa                                                                                             >  1:624860/1‑148 (MQ=255)
                                               tACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGCTGTCGGAGaa                                                                                             >  1:125387/1‑148 (MQ=255)
                                                aCATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTGTTATTTGGTGTTGTCGGAGAAg                                                                                            >  2:541813/1‑148 (MQ=255)
                                                aCATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGTTGTTGTCGGAGAAg                                                                                            >  2:937887/1‑148 (MQ=255)
                                                  aTCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTCTTATTTGGTGTTTTCGGAGAAGCg                                                                                          >  1:372727/1‑148 (MQ=255)
                                                  aTCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCg                                                                                          >  1:549570/1‑148 (MQ=255)
                                                    cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTc                                                                                        <  2:802228/148‑1 (MQ=255)
                                                    cGTTATCCATTGTCGGACGGGTGGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTc                                                                                        <  2:492277/148‑1 (MQ=255)
                                                     gTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGGGGCGttt                                                                                       >  2:377639/1‑146 (MQ=255)
                                                          tttttgttgGACTGTGTGTTCAGGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCttt                                                                                  <  2:747268/145‑1 (MQ=255)
                                                          cgaTTGTCGGACTGTTTGTTGATGCATCTACACTAGATTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCTATTATTTGGTGTTGTCGGAGAAGCGTCTGCttt                                                                                  <  2:947655/146‑1 (MQ=255)
                                                          ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGGAGCGTCTGCttt                                                                                  >  2:400848/1‑148 (MQ=255)
                                                          ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCttt                                                                                  >  1:523099/1‑148 (MQ=255)
                                                           cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTg                                                                                 <  1:869688/148‑1 (MQ=255)
                                                           cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTg                                                                                 <  1:795815/148‑1 (MQ=255)
                                                              tttCGGACTGTTTGTTGATGCATCCACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                              <  2:296453/146‑1 (MQ=255)
                                                                        tttgttGATTCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCACTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTCTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcg                                                                    <  2:552282/148‑1 (MQ=255)
                                                                             tgATGCATTTACACTAGCATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa                                                               <  2:22053/148‑1 (MQ=255)
                                                                             tgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa                                                               <  1:797461/148‑1 (MQ=255)
                                                                             tgATGCATCCAAACCAGAATCGTTATTAGCTTGCCGTTTATCATTTGCATCATCAGTTGCCGATGTTACTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa                                                               <  1:52813/148‑1 (MQ=255)
                                                                                tGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGTATCATCAGTTGCTGATGTTACTGTTGTTTAACCTGTTGCCGCCTCTCCATTAGTGGGTGTTTTCGGGGAAGCGGCTGCTTTGCCATTAGCTTTCGTCTCAGAt                                                            >  2:333566/1‑148 (MQ=255)
                                                                                  cATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACCATTATTTGCTTTTTTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATAc                                                          <  2:164383/148‑1 (MQ=255)
                                                                                    tCTACACTAGAATTGTTATTAGCTTGCCGTTTATCATTTGCAACATTACAATGTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt                                                        <  1:470187/148‑1 (MQ=255)
                                                                                     cTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCTTTATACGGTAGCCTTTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt                                                       >  1:433125/1‑148 (MQ=255)
                                                                                     cTACACTAGAATTGTTACTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAGGCGTCTGCTTTGCCCTTAGCTGTCGTCTCAGATACGtt                                                       >  2:433195/1‑148 (MQ=255)
                                                                                      caccccaGAATTGTTATCAGCTTGCGGTTTATCATTTGCACCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGGGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTa                                                      <  2:767566/142‑1 (MQ=255)
                                                                                       acacTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAg                                                     >  2:809284/1‑148 (MQ=255)
                                                                                           tctaaTTGTTATTAGCTTGCGGTTTATCATTTGCATTAGCTGTTGCTGGTGTTACTGTTGTTTCACCTGTTGCCGCTTCTCTATTATTTGGTGTTGTCGGAGACGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg                                                 <  2:413274/145‑1 (MQ=255)
                                                                                           tAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg                                                 <  1:37293/148‑1 (MQ=255)
                                                                                           tAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg                                                 <  1:773766/148‑1 (MQ=255)
                                                                                              aaTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTcc                                              <  2:456642/148‑1 (MQ=255)
                                                                                               aTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCa                                             >  1:95863/1‑148 (MQ=255)
                                                                                                  gTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGtt                                          >  1:760820/1‑147 (MQ=255)
                                                                                                    tattaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtt                                        >  1:164783/1‑148 (MQ=255)
                                                                                                      ttaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtttt                                      <  1:81249/148‑1 (MQ=255)
                                                                                                      ttaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtttt                                      <  1:556034/148‑1 (MQ=255)
                                                                                                       taGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc                                     >  1:540191/1‑148 (MQ=255)
                                                                                                          cTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTgg                                  >  2:445985/1‑148 (MQ=255)
                                                                                                           ttGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt                                 >  2:969680/1‑148 (MQ=255)
                                                                                                           ttGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCAGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCGATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATGTTCTGgt                                 >  2:771354/1‑148 (MQ=255)
                                                                                                             gCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtgt                               >  1:105359/1‑148 (MQ=255)
                                                                                                                gTTTATCTTTTGCATCATCAGTTGCTGATGTTCCTGTTGTTTCACCTGTTGCCTCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGc                            <  2:338889/148‑1 (MQ=255)
                                                                                                                gTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGc                            >  2:158738/1‑148 (MQ=255)
                                                                                                                    aTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTa                        <  2:817592/148‑1 (MQ=255)
                                                                                                                      cATTTGCATCATCAGTTGCTGATGTTAATGTTGTTTCACCTGTTGCCGCATAACTATTAGTTGGTATTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGGTAGGTTGGCCAGTATTTTCTGGTGTTGCATTAGc                      >  1:603459/1‑148 (MQ=255)
                                                                                                                         ttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTGGCGtt                   >  2:650485/1‑148 (MQ=255)
                                                                                                                         ttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAtt                   >  1:831775/1‑148 (MQ=255)
                                                                                                                         ttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAtt                   >  1:5500/1‑148 (MQ=255)
                                                                                                                         ttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTCTCCAGTATTTTCTGGGGTTGCATTAGCAtt                   >  1:111457/1‑148 (MQ=255)
                                                                                                                              tcatcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAt              >  1:645943/1‑148 (MQ=255)
                                                                                                                               catcaGTTGCGGCTGTTACTGTTGTGTCCCCTGTTGCCGCATCACTATTATTTGGGGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt             <  1:12532/148‑1 (MQ=255)
                                                                                                                                atcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCGGTATTTTCTGGTGTTGCATTAGCATTTGAAttt            >  1:767778/1‑148 (MQ=255)
                                                                                                                                   aGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCt         >  1:637996/1‑148 (MQ=255)
                                                                                                                                    gTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg        >  2:197625/1‑148 (MQ=255)
                                                                                                                                      tGCTGATGTTCCTTTTGTTTCACGTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGtt      <  2:533540/148‑1 (MQ=255)
                                                                                                                                       gCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGTTg     <  1:809284/148‑1 (MQ=255)
                                                                                                                                        cTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGTATTTGCTGTTGc    >  2:575279/1‑148 (MQ=255)
                                                                                                                                        cTGATGTTACTGTTATTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAGGCGTCTGCTTTGCCATTAGCTGTCGTGTCAGGTAAGTTAGGGTGGCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCGTTTGc    >  2:232325/1‑148 (MQ=255)
                                                                                                                                         tGATGTTAGTGTTGTTTCACCTTTGGCCGCATCCCTTTTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTATCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAGTAGCATTTGAATTTGCTGTTGca   <  2:211182/148‑1 (MQ=255)
                                                                                                                                         tGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGTTGca   >  2:189923/1‑148 (MQ=255)
                                                                                                                                         tGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGTTGca   <  1:649141/148‑1 (MQ=255)
                                                                                                                                         tGATGGTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCACTAGCATTTGAATTTGCTGTTGca   <  1:591838/148‑1 (MQ=255)
                                                                                                                                         ggATGTTCGTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGTTGca   <  2:881015/147‑1 (MQ=255)
                                                                                                                                          gATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTCTTTGGTGTTGTCGGAGCAGCGTCTGCTTTGCGATTAGCTGTCGTCTCAGATACGTTAGGGTTTCCAGTATTTTGTGGTGTGGCGTTAGCATTTGGATTTGCTGTTGcat  >  1:49138/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TTATCAGTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGTTGCAT  >  USA300TCH1516_ALE/2279561‑2279846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: