Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,856,087	T→A	P80P (CCT→CCA)	rpoA_2 →	DNA‑directed RNA polymerase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,856,087	0	T	A	100.0%	313.8 / NA	98	P80P (CCT→CCA)	rpoA_2	DNA‑directed RNA polymerase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (50/48);  total (50/48)

CTAATGGAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCTAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTCAAGAACTA  >  USA300TCH1516_ALE/2855944‑2856231                                                                                                                                                |                                                                                                                                                 ctAATGGAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAAc                                                                                                                                              <  1:915074/148‑1 (MQ=255)  tAATGGAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACg                                                                                                                                             >  1:715188/1‑148 (MQ=255)  tAATGGAAATGCATGGCGTTGGTCCTACAGCTATATCAATATTGGAACAAGCTTTATTTCAGCCCCAAATACATTTTATAACGGAAGTTCAGTCATCATTACCATTTAAGTTAACAGGAGATGTGTCATGCAATTATGCTCCAAAAct                                                                                                                                             >  2:228858/1‑147 (MQ=255)     tGGAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGCTGTGTCATGCAATCATGCGCCAAAACGTca                                                                                                                                          >  2:618957/1‑148 (MQ=255)     tGGAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTca                                                                                                                                          >  1:3019/1‑148 (MQ=255)      ggAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTcaa                                                                                                                                         >  2:221734/1‑148 (MQ=255)       gagaTGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAACACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTcaac                                                                                                                                        >  2:835250/1‑148 (MQ=255)        agaTGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTAAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTAATGCAATCATGCGCAAAAACGTcaaca                                                                                                                                       >  2:114807/1‑148 (MQ=255)         gaTGCATGGCGTTTGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTcaacaa                                                                                                                                      <  2:753534/148‑1 (MQ=255)         gaTGCATGGCATTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTcaacaa                                                                                                                                      <  2:841096/148‑1 (MQ=255)         aaTGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTcaacaa                                                                                                                                      <  2:841524/147‑1 (MQ=255)          aTGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTCCATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAAAAGGAGATGTGTCATGCATTCATGCGCCAAAACGTCAACaaa                                                                                                                                     >  1:201503/1‑148 (MQ=255)          aTGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACaaa                                                                                                                                     <  2:340949/148‑1 (MQ=255)          aTGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACaaa                                                                                                                                     <  1:97732/148‑1 (MQ=255)              aTGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTCCCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGaa                                                                                                                                 >  1:578550/1‑147 (MQ=255)                  ggTTGGTCCTTAATCTATATCAAGATTGGAAAAATCTTTATTCCAGCACCAATTACATTTTAAAACGGAAGTTTAGTCATCATTAACTTTTAAGTTAACAGTAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGAGAGAt                                                                                                                             <  2:110517/147‑1 (MQ=255)                  ctttGGTCCTAAAGCTATATCAATATTGAAACAAGTGATAGTTTAGTACCAACTACATGTTAAAAGGGAATTTTAGTCATCATTACCTTTTAAGTGAACAGCAGATGTGTCATGCAATCATGCGCCAAAGCGTGAACAAGTGATAGAt                                                                                                                             <  2:140876/146‑1 (MQ=255)                  ccttGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGAt                                                                                                                             <  2:287973/146‑1 (MQ=255)                  cGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGAt                                                                                                                             <  1:260536/148‑1 (MQ=255)                         ccTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAg                                                                                                                      <  1:89557/148‑1 (MQ=255)                          cTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGCGGGTGCGGGCAATAGCGAACCACAACGTCAACAAATGATAGATTTTATAgt                                                                                                                     >  1:225865/1‑148 (MQ=255)                               gCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAACTGCAGTCATCATTACCTTTTAAGGTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTa                                                                                                                >  2:702705/1‑148 (MQ=255)                                    atCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACGGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATTATAGATTTTATAGTTGTTAcagca                                                                                                           <  2:380972/148‑1 (MQ=255)                                    atCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTAcagca                                                                                                           <  2:629043/148‑1 (MQ=255)                                      cAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACagcagc                                                                                                         >  2:675412/1‑148 (MQ=255)                                        atatTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCAt                                                                                                       >  2:729169/1‑148 (MQ=255)                                            tGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGa                                                                                                   >  2:567204/1‑148 (MQ=255)                                             gtaataaGGTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTGCAGTCATCCTCTTTTTTCAACTTAACAGGGGATGGGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGat                                                                                                  <  1:721501/143‑1 (MQ=255)                                             ggAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATCGATTTTATAGTTGCTACAGCAGCATTAGat                                                                                                  >  1:268428/1‑148 (MQ=255)                                             ggAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGat                                                                                                  >  1:412547/1‑148 (MQ=255)                                             ggAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGat                                                                                                  >  1:870366/1‑148 (MQ=255)                                                aCAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACCGGAGCTGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGctaat                                                                                               >  2:452263/1‑146 (MQ=255)                                                 cAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATATTTGTTACAGCAGCATTAGATATTg                                                                                              >  2:645972/1‑148 (MQ=255)                                                 cAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTg                                                                                              >  2:145467/1‑148 (MQ=255)                                                 cAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGATCAGACAACACTACCGTTGAAGTGACCAGTAGAGTTGTCATTCAATCATGCGCCAAGACGTCATCCAGTGATAGGTTTGATAGTGCTGACAGCAGCAGTAGATAttt                                                                                              <  2:251817/148‑2 (MQ=255)                                                  aaGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGa                                                                                             >  2:343892/1‑148 (MQ=255)                                                  aaGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGa                                                                                             >  2:925382/1‑148 (MQ=255)                                                       ttATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAattat                                                                                        <  1:172944/148‑1 (MQ=255)                                                       ttATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAattat                                                                                        >  2:480469/1‑148 (MQ=255)                                                       taaTTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAattat                                                                                        <  1:466083/146‑1 (MQ=255)                                                        tATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAattatt                                                                                       >  1:384277/1‑148 (MQ=255)                                                             cAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGtt                                                                                  >  2:659268/1‑148 (MQ=255)                                                              aGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTc                                                                                 >  2:930792/1‑148 (MQ=255)                                                                cACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCAc                                                                               >  2:53970/1‑148 (MQ=255)                                                                 aCCAACTAAATTTTAAAACGGTAGTTCCGTAATCATTNCCGTTTAAGTTATCAGGAGATGTGTTATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGCTATTGAATTATTACGTTCACt                                                                              <  2:679038/148‑1 (MQ=255)                                                                    aattACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGt                                                                           <  2:202141/145‑1 (MQ=255)                                                                     aCTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGtt                                                                          <  2:602142/148‑1 (MQ=255)                                                                     aCTACATTCTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCATCATTAGATATTGAATTATTACGTTCACTGGtt                                                                          <  2:703482/148‑1 (MQ=255)                                                                       tACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTCAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTac                                                                        <  2:207432/148‑1 (MQ=255)                                                                         cATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTacaa                                                                      <  1:121115/148‑1 (MQ=255)                                                                            tttAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGGTCACTGGTTACAACAg                                                                   >  1:783796/1‑148 (MQ=255)                                                                             ttAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGa                                                                  >  1:38869/1‑148 (MQ=255)                                                                             ttAAAACGGAAGTTCAGTCATCATTACCTTCTCAGCAACCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGa                                                                  >  1:482079/1‑148 (MQ=255)                                                                                aaaCGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatt                                                               >  2:68011/1‑148 (MQ=255)                                                                                  aCGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTCCAAAGGAattta                                                             >  2:880451/1‑148 (MQ=255)                                                                                  aCGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatttt                                                             >  2:983797/1‑147 (MQ=255)                                                                                  aCGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAattta                                                             <  2:498237/148‑1 (MQ=255)                                                                                   cGGAAGTTCAGTCATCATTACGTTTTGAGTTAGCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatttat                                                            >  1:546788/1‑148 (MQ=255)                                                                                   cGGAAGTTCAGTCATCATTACCTTTTAAGTAAAAAGGGTATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatttat                                                            >  2:703870/1‑148 (MQ=255)                                                                                    ggAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatttatt                                                           <  2:706952/148‑1 (MQ=255)                                                                                    ggAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatttatt                                                           <  1:586482/148‑1 (MQ=255)                                                                                    ggAAGTTCAGTCATCATTACCTTTGAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatttatt                                                           <  2:820208/148‑1 (MQ=255)                                                                                          tCAGTCATCCTTACCTTTTAAGTTAACTGGAGATGTGTCATGCAATCATGCGCCAACACGTCAACAAATGATAGATTTTATAGTTGTTCCAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCt                                                     >  2:608126/1‑148 (MQ=255)                                                                                               catcatTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTccc                                                <  1:221734/148‑1 (MQ=255)                                                                                               catcatTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATAATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTccc                                                >  2:409576/1‑148 (MQ=255)                                                                                               catcatGACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCAAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATGAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTccc                                                <  1:38555/148‑1 (MQ=255)                                                                                                atcatTACCTTTTAAGTTAAAAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTGGGTCTGTCCCt                                               <  2:687615/148‑1 (MQ=255)                                                                                                     tACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCg                                          <  1:130511/148‑1 (MQ=255)                                                                                                       ccTTTTAAGTTAACAGGTTATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCt                                        <  1:618957/148‑1 (MQ=255)                                                                                                        gatggaaGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCtt                                       <  2:244628/143‑1 (MQ=255)                                                                                                        cTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCtt                                       <  2:436735/148‑1 (MQ=255)                                                                                                         ttttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCttt                                      <  2:402606/148‑1 (MQ=255)                                                                                                         ttttAAGCTAACAGTAGAGGTGTCATGCAATCATGCGCCAAACCGTCAACAAATGCTAGATTTTATAGGTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCttt                                      <  2:598535/148‑1 (MQ=255)                                                                                                          tttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg                                     <  1:930792/148‑1 (MQ=255)                                                                                                          tttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg                                     <  1:404057/148‑1 (MQ=255)                                                                                                          tttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTGATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg                                     <  2:431998/148‑1 (MQ=255)                                                                                                          tttAAGGTAACAGGAGATTTGTCATGCAATCATGCGCCAAAACGGCAACAAATTATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATGACGGTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg                                     <  1:203157/148‑1 (MQ=255)                                                                                                           ttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGa                                    >  1:965186/1‑148 (MQ=255)                                                                                                           ttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGa                                    >  2:42535/1‑148 (MQ=255)                                                                                                           ttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGa                                    >  2:507728/1‑148 (MQ=255)                                                                                                            aaaGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGAc                                   >  2:259328/2‑148 (MQ=255)                                                                                                             aaGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACa                                  >  2:8607/1‑148 (MQ=255)                                                                                                                  aaCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATAc                             >  2:463427/1‑148 (MQ=255)                                                                                                                  aaCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATAAAc                             >  2:378163/1‑148 (MQ=255)                                                                                                                   aCAGGAGCTGTGTCATGCAATCATGCGCCAAAACGTCAACCAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCCGGCCGCTTTGACATATACg                            <  1:702717/148‑1 (MQ=255)                                                                                                                    cAGGCGGTGTGTCATGCAATCATGCTCCAAAACGTCAACAAATGTTAGAGTTTATAGTTGTTACAGCAGCATTAGATATTGGATTATTACGGTCACTGGTTACAACAGAATTTATTTGGTCTGGGCCTGGCCGCTTTGACATATACgg                           <  1:496919/148‑1 (MQ=255)                                                                                                                    cAGGAGATGTGTCATGCAATCGTGCGGCAAAATGTCAACAAATGATAGATTGTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACgg                           >  1:544875/1‑148 (MQ=255)                                                                                                                    cAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACgg                           <  1:925382/148‑1 (MQ=255)                                                                                                                      ggAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGAc                         >  1:217552/1‑148 (MQ=255)                                                                                                                      ggAGATGTGTCATGCAATCATGCGCCAAAACGTCAAAAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGAc                         >  1:142253/1‑148 (MQ=255)                                                                                                                       gagaTGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGCTCAAACAGCATCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGAcc                        <  1:721170/148‑1 (MQ=255)                                                                                                                        agaTGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCt                       >  2:379918/1‑148 (MQ=255)                                                                                                                        agaTGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTCTATAGCTGTTACAGCCACATTAGATATTAAATATTTACGTTCAGTGGTTAAAGCAGAATTTATTTGGGTTGTCCCTGGCCCCTTTGACATATACGGAccc                       >  2:726570/1‑147 (MQ=255)                                                                                                                         gaTGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTc                      <  1:404331/148‑1 (MQ=255)                                                                                                                           tgtgTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCaa                    <  1:948537/148‑1 (MQ=255)                                                                                                                                cATGCAATCATGCGCCAAAACGTAAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATAtt               >  1:496104/1‑148 (MQ=255)                                                                                                                                      aTCATGCGCCAAAACGTCAACAAATGCTAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTCCACTGGTTACAACAGAATTTATTTGTTCTGTCACGGGCTTCTTTGACATATACGGACCTCTAATATTAATTCa         <  1:729169/148‑1 (MQ=255)                                                                                                                                             gcCAAAACGTCAACGAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTCAAGAACTa  <  2:225865/148‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                 CTAATGGAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCTAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTCAAGAACTA  >  USA300TCH1516_ALE/2855944‑2856231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑