| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 295,322 | G→C | G68A (GGT→GCT) | tarJ' → | Ribulose‑5‑phosphate reductase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 295,322 | 0 | G | C | 99.0% | 329.6 / NA | 99 | G68A (GGT→GCT) | tarJ' | Ribulose‑5‑phosphate reductase 2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (53/44); minor base T (0/1); total (54/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTGATATTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGGTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTATGATGGT > USA300TCH1516_ALE/295179‑295464 | tGTTGATATTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGaa > 2:150837/1‑148 (MQ=255)tGTTGATATTTATGGTAATCATGTCATCGTAAGACCTGTATCCTTGTCTATTTGTGCAGCTGATCATAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGATGCGGTTGCTGag > 2:383191/1‑147 (MQ=255) gTTGATATTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAg < 1:9046/148‑1 (MQ=255) tGATATTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGtt > 1:723962/1‑148 (MQ=255) tatTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTGGTa > 2:27028/1‑148 (MQ=255) tatTTATGGTAATCATGTCATAGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAAATACCACTGTCAGCAGTTCATGAAACAGTTGAGGAAGTTgaa > 2:748687/1‑146 (MQ=255) tttATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGAAtt > 2:561578/1‑148 (MQ=255) ttATGGTAAGCTGTTCATTGGAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTAttt < 1:125369/148‑1 (MQ=255) tATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGGTGCTGAAGTTGTATTTg > 1:868601/1‑148 (MQ=255) tGGTAATCATGTCAACGTAAGACCTTTATACTTGTCTATTAGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACAACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGAt < 2:787380/148‑1 (MQ=255) ggTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGACGAGATGAAAATGTACTACGCAAAAAATTAACAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGATTTTGATa > 1:79842/1‑148 (MQ=255) ggTAATCATGTAATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATa > 2:856436/1‑148 (MQ=255) aaTCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGCATTTGATAGTa > 1:44733/1‑148 (MQ=255) aaTCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATGAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTa > 2:266061/1‑148 (MQ=255) aTCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTaa < 1:265642/148‑1 (MQ=255) tCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGt < 1:983225/148‑1 (MQ=255) aTCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTAt < 1:870173/148‑1 (MQ=255) aGACCTTTATTCTTGTCTATTTGTGCAGCTGATCAACGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGaaa < 1:551028/148‑1 (MQ=255) gACCTTTATACTTGTCGATTGGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGGGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGaaaa < 2:251522/148‑1 (MQ=255) aCCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATTAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGaaaaa < 2:178292/148‑1 (MQ=255) aCCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGaaaaa < 1:113767/148‑1 (MQ=255) cTTTATACTTGTCTATTTGTGCAGCTGTTCCGAGGTGTTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAgg > 1:982474/1‑148 (MQ=255) cTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAgg > 1:615125/1‑148 (MQ=255) ttATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTa > 1:447390/1‑148 (MQ=255) aCTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTCCGaa > 2:439139/1‑148 (MQ=255) aCTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGaa > 1:119332/1‑148 (MQ=255) aCTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGATCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTCGAAAAAGGTACGaa > 1:711092/1‑148 (MQ=255) cTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGCAAAAGGTACGaac > 2:244099/1‑147 (MQ=255) tGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTGGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAACGGGACGAAagt > 1:49276/1‑148 (MQ=255) tGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAagt > 2:484302/1‑148 (MQ=255) tGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTGCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAagt > 1:38340/1‑148 (MQ=255) cTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTAAGAAagtagt > 2:434808/1‑148 (MQ=255) ttGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTACAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATg > 2:352956/1‑148 (MQ=255) ttGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATg < 1:992488/148‑1 (MQ=255) gCTGATCACAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGa < 1:873709/148‑1 (MQ=255) gCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGa < 1:136000/148‑1 (MQ=255) cTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTGCTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGTAAGTAGTAATGGTACCGaa < 1:702497/148‑1 (MQ=255) cTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGGAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGaa < 1:924364/148‑1 (MQ=255) cTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGaa < 2:778069/148‑1 (MQ=255) tCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATaca < 1:565461/148‑1 (MQ=255) cAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATacac > 1:899683/1‑148 (MQ=255) aaGGTATTACAAAGGTCGAAGAGATTAAAATGTAATACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCt < 2:72613/148‑1 (MQ=255) gttATTACATAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGACAGTAGTAATGGTACCGAATACACCTAc < 1:530797/146‑1 (MQ=255) acacAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCa < 1:435453/148‑1 (MQ=255) acacAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCa > 1:31228/1‑148 (MQ=255) acaGGTCGAAGAGATGAAAATGTACTACGCAGAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCaaa > 2:552709/1‑147 (MQ=255) acaGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATATGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAAc > 2:650905/1‑148 (MQ=255) aGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAAcat < 1:810166/148‑1 (MQ=255) aaGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCAtatt > 2:739895/1‑148 (MQ=255) agaTGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGCTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATattatt < 1:201082/148‑1 (MQ=255) agaTGAAAATGTACTACGCAAAAAATTAACAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGGGCAACATCATattatt < 2:364549/148‑1 (MQ=255) ataaaaaTGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGc < 2:966251/145‑1 (MQ=255) aTGAACATGTACTACGCCAAAAATCACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGc < 2:713692/148‑1 (MQ=255) gAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACCTCATATTATTGCgg < 2:637242/148‑1 (MQ=255) tactacGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGGAGTTGTATTTGATAGTAAAGGGGTAGTTGAAAAAGGGACGATAGTAGTAATGGTACCGAATACACCTGCAGAGCAACATCATATTATTGCGGAGAATTa < 1:725208/148‑1 (MQ=255) tactacGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTa < 2:125705/148‑1 (MQ=255) actacGCAAAAAATTACCAATGTTATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTaa > 1:495015/1‑147 (MQ=255) actacGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCGGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCGACAGAGCAACATCATATTATTGCGGAGAATTAc > 1:930367/1‑148 (MQ=255) cAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACCTCATATTATTGCGGAGAATTACTTAGcc > 2:221229/1‑148 (MQ=255) cAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGGGAATTACTTAGcc > 2:179935/1‑148 (MQ=255) aaaaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCct < 1:945443/148‑1 (MQ=255) aaaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctc > 2:711565/1‑148 (MQ=255) aaaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTAAATTTGATAGTAAAGGGGGATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTAGAGAGCAACATCATATTGTTGCGGAGAATTAATTGGcccc > 2:135212/1‑146 (MQ=255) aaaaTTACCAATGTCATTAGTTCATGCACCTCTTGTGGCACTGGTGTTTGTTAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGCATTACTTAGCctct < 2:206821/148‑1 (MQ=255) aaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctct > 2:721686/1‑148 (MQ=255) aaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctct > 1:304917/1‑148 (MQ=255) aaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctct < 2:298596/148‑1 (MQ=255) aaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctca > 2:627276/1‑147 (MQ=255) aaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGGAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctct < 1:333443/148‑1 (MQ=255) aaTTCCCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTTTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAg < 1:182411/148‑1 (MQ=255) ttACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTGCTTAGCCTCTAGtt > 2:919509/1‑148 (MQ=255) aTGGCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGGAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTa < 1:38983/148‑1 (MQ=255) tGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAg < 1:677410/148‑1 (MQ=255) ggcATGAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGa < 2:62748/146‑1 (MQ=255) gTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTata > 1:368791/1‑146 (MQ=255) cATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGTGCATTACTTAGCCTCTAGTTATTTTatatt > 2:630618/1‑147 (MQ=255) cATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTCTTTTAGATc > 1:79393/1‑148 (MQ=255) cATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATc > 1:149131/1‑148 (MQ=255) cATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTAGTTTAGATc > 2:785211/1‑148 (MQ=255) ttAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTa < 2:31228/148‑1 (MQ=255) tAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAg > 1:160845/1‑148 (MQ=255) tAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAg > 1:140084/1‑148 (MQ=255) gTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTCCTTAGCCTCTAGGTATTTTCGATCTGGTg > 2:180550/1‑148 (MQ=255) ttCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTgg < 2:447390/148‑1 (MQ=255) tCATGACGCTGTTGCTGAAGTTGTATTTGCTAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGt < 1:164614/148‑1 (MQ=255) tCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGt > 1:984748/1‑148 (MQ=255) tCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGt > 1:50459/1‑148 (MQ=255) tCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGt > 1:328300/1‑148 (MQ=255) tCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGt < 1:561578/148‑1 (MQ=255) cATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGtt > 2:584499/1‑148 (MQ=255) cATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGtt < 2:723962/148‑1 (MQ=255) cATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTAATTAGACTCTAGTTATTTTAGGTGTGGTGGtt > 2:537861/1‑148 (MQ=255) tGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTat > 1:899117/1‑148 (MQ=255) tGAAGCTGTTGCTGAAGTGGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCGCTAGACAATTTAGGTCTAGTGGTTag > 2:509247/1‑147 (MQ=255) gAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTCGACAAAGGTACGCACGCAGTACTGGCACCGCATCCCCATACAGACCAACATCATATCATTGCGGAGCAGCACGTAGCCTCTAGTGATCTTAGATCTAGTGGTTatg > 2:577019/1‑148 (MQ=255) cTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTATGATgg < 1:856436/148‑1 (MQ=255) tGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCATCATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTATGATGGt < 1:383191/148‑1 (MQ=255) tGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTATGATGGt < 2:38340/148‑1 (MQ=255) tGTTGCTGAAGTTGTATTTGATAGTAAAGAGGTATTTGAAAAATGTACTAAAGTAGTAATTGTACCGAATACACCTACATAGCTACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGGTATGATGGt < 2:49276/148‑1 (MQ=255) | TGTTGATATTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGGTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTATGATGGT > USA300TCH1516_ALE/295179‑295464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |