BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	479,553	G→A	A194T (GCA→ACA)	USA300HOU_RS02280 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	479,553	0	G	A	100.0%	416.8 / NA	127	A194T (GCA→ACA)	USA300HOU_RS02280	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (56/71); total (56/71)

Only 100 of 127 total aligned reads displayed.

AGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCAAGC  >  USA300TCH1516_ALE/479415‑479692

aGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTAGCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGc                                                                                                                                    <  2:530545/148‑1 (MQ=255)

  tGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCAt                                                                                                                                  >  2:900941/1‑148 (MQ=255)

          taataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCa                                                                                                                          >  2:961997/1‑148 (MQ=255)

          taataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCa                                                                                                                          >  1:459964/1‑148 (MQ=255)

          taataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCa                                                                                                                          >  1:551510/1‑148 (MQ=255)

          taataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCa                                                                                                                          >  2:599054/1‑148 (MQ=255)

             taGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTGTATCGATCAATTTCTGTCGAGATGGACAATGCCGAGGAGTGAGAAAAGTTCTTACCATACATG‑GTTGCATTTAGCGCaaaa                                                                                                                       >  2:819758/1‑146 (MQ=255)

             taGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACa                                                                                                                       <  1:766772/148‑1 (MQ=255)

               gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATg                                                                                                                     <  2:177858/148‑1 (MQ=255)

                aTGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGa                                                                                                                    <  2:368916/148‑1 (MQ=255)

                    aCAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCaa                                                                                                                >  1:23042/1‑147 (MQ=255)

                    aCAAGGTGAACCATTTCGCGAACATGTGCATCGTCAAATCTTTAAATGGACCATACTTTATATCGATCAATTTCTTTTGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACAAG‑GTTGCATTTAGCGTAACATGACCAt                                                                                                                <  2:588022/148‑1 (MQ=255)

                       aGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGt                                                                                                             <  2:437475/148‑1 (MQ=255)

                       aGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGt                                                                                                             <  1:738677/148‑1 (MQ=255)

                         gTGAGCCACTTAGCTAACTACTGATTCGTCAAATTATTATGTGGACGGCAAATCATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGttt                                                                                                           <  1:469847/148‑1 (MQ=255)

                         gTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGttt                                                                                                           <  1:810076/148‑1 (MQ=255)

                         gTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGttt                                                                                                           >  1:553355/1‑148 (MQ=255)

                          tGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGtttt                                                                                                          <  1:106616/148‑1 (MQ=255)

                           gAGCCATTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTa                                                                                                         <  2:506350/148‑1 (MQ=255)

                              ccACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCCAAGTTTTTACCATACATG‑GTTGCCTTTAGCGCAACATGACCATAGTTTTACTa                                                                                                      >  2:84411/1‑148 (MQ=255)

                               cACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCCCAACATGACCATAGTTTTACTaa                                                                                                     <  1:498244/148‑1 (MQ=255)

                                aCTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTaac                                                                                                    >  1:83128/1‑147 (MQ=255)

                                aCTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTAGATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTaaa                                                                                                    >  1:628761/1‑148 (MQ=255)

                                aCTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTATCTATCGATAAATTTCTATCGAGTTGGAAAATGCCGAAGCGTGAGCAAAGTTTTTACATTACATG‑GTTTCATTTAGAGAAACATGCCGATAGTTTTACTaaa                                                                                                    >  2:487597/1‑148 (MQ=255)

                                     gctaaCAAGTGAATCGTCAAACGATTAAATGGACGAAGCCTTATATCGGTCAATTTCTATCGAGTTGGACAATGCCTAAGGGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATTACCATAGTTTTACTAAAGcaca                                                                                               <  2:78090/145‑1 (MQ=255)

                                     gCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGcaca                                                                                               <  1:810826/148‑1 (MQ=255)

                                     gCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGcaca                                                                                               <  1:652844/148‑1 (MQ=255)

                                      cGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGAACAg                                                                                              >  1:963501/1‑148 (MQ=255)

                                       gAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgc                                                                                             >  2:749161/1‑148 (MQ=255)

                                        aacgattGAGTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACACG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcg                                                                                            <  2:925011/142‑1 (MQ=255)

                                           aaGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCa                                                                                         >  2:426654/1‑148 (MQ=255)

                                           aaGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCa                                                                                         <  1:207081/148‑1 (MQ=255)

                                            aGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCGTTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCaa                                                                                        >  1:417504/1‑148 (MQ=255)

                                               gAATCGTCAAATGATTAAATGGACGAAACTTTATATCTATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTCTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTg                                                                                     <  2:111042/148‑1 (MQ=255)

                                               gAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTg                                                                                     <  2:339684/148‑1 (MQ=255)

                                                aaTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTCCTAAAGCACAGCGCCAAGTGa                                                                                    >  2:541734/1‑148 (MQ=255)

                                                    gTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTaa                                                                                <  1:965875/148‑1 (MQ=255)

                                                       aaaTGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACACTGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCGTTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAgg                                                                             >  1:557851/1‑148 (MQ=255)

                                                            aTTAAATGGACGAAACTTTATGTCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GATGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGAGAGTAAAGGCTAAc                                                                        >  2:681126/1‑148 (MQ=255)

                                                            aTTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTAc                                                                        >  1:687218/1‑148 (MQ=255)

                                                            aTTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTAc                                                                        >  2:866022/1‑148 (MQ=255)

                                                            aTTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTAc                                                                        <  2:880783/148‑1 (MQ=255)

                                                            aTTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTAc                                                                        >  1:355814/1‑148 (MQ=255)

                                                            aTTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTAc                                                                        <  2:910753/148‑1 (MQ=255)

                                                               aaaTGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCa                                                                     <  1:749161/148‑1 (MQ=255)

                                                               aaaTGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCa                                                                     >  1:830707/1‑148 (MQ=255)

                                                                  tGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCCACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTCAAGGCTTACCCAAta                                                                  >  2:48627/1‑147 (MQ=255)

                                                                   ggACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGa                                                                 >  1:574227/1‑148 (MQ=255)

                                                                   ggACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGa                                                                 >  2:176733/1‑148 (MQ=255)

                                                                   ggACGAAACTCTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGGTTTTACCATACATG‑GTTTCATTTAGCGCAACATGAACATAGTTTTACTAATGCACAGCGCCAAGTGATTACCTGCTTTCCCAAttt                                                                 >  2:176745/1‑146 (MQ=255)

                                                                     gcGAAACTTTATATCGATCAATTTCTATCGAGTTAGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATc                                                               <  2:300457/147‑1 (MQ=255)

                                                                      cGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATcc                                                              >  2:719164/1‑148 (MQ=255)

                                                                       gAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCt                                                             >  1:301246/1‑148 (MQ=255)

                                                                          aCTCTATATCCATCAATTTCTATCGAGTTTGACAATGCCGAAGCGTGAGCAAAGTCTTTACCATACATA‑GCTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGaa                                                          <  1:100722/148‑1 (MQ=255)

                                                                           cTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACCGCGCCAAGTGATTAAAGGCTTACCCAATAATCCTGaaa                                                         >  1:18306/1‑148 (MQ=255)

                                                                            tttATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAAt                                                        <  1:907305/148‑1 (MQ=255)

                                                                            tttATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAAt                                                        <  1:863925/148‑1 (MQ=255)

                                                                            tttAGACCGATCAATTTCCATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATTACCATCGTTGTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAAt                                                        <  2:409777/148‑1 (MQ=255)

                                                                                tatTGAGCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACg                                                    <  2:630162/148‑1 (MQ=255)

                                                                                tatCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACg                                                    <  1:503705/148‑1 (MQ=255)

                                                                                  tCGATCACTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GCTGCATTTAGCGCAACAGGACCATTGTTTTACTACAGAATCACGCCAAGTGATTAGAGGCTTACCCAATTAGCCTGAAAAGCCGAt                                                  >  2:233336/1‑148 (MQ=255)

                                                                                  tCGATCAATTTCTATCGAGTTGGCCACTGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGAt                                                  >  1:555733/1‑148 (MQ=255)

                                                                                    gATCAATTTCTATCGAGTTGGACAATGGCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTCACCCAATGATCCTGAAAGGACGATag                                                >  1:601080/1‑148 (MQ=255)

                                                                                      tCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATaggg                                              >  1:577223/1‑146 (MQ=255)

                                                                                      tCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGACCCTGAAATGACGCTagag                                              >  2:203471/1‑148 (MQ=255)

                                                                                      tCAATTTCTATCGAGTTGGACAAAGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGCCGATagcg                                              >  1:592366/1‑146 (MQ=255)

                                                                                           ttCTATCGAGTGCGCCAATGCCGAAGCGGTAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGACTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGt                                         <  1:63791/148‑1 (MQ=255)

                                                                                            tCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTa                                        <  2:830707/148‑1 (MQ=255)

                                                                                                tCGAGTTGGACAATGCCGAAGCGTGCGCAAAGCTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGACCCTGAAATGACGATAGAGTCAGAATTaa                                    >  2:215033/1‑148 (MQ=255)

                                                                                                   aGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTc                                 <  2:793687/148‑1 (MQ=255)

                                                                                                   aGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTc                                 <  2:879061/148‑1 (MQ=255)

                                                                                                    gTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGAATAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCa                                >  2:657112/1‑148 (MQ=255)

                                                                                                     ttGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAt                               <  2:324258/148‑1 (MQ=255)

                                                                                                       ggACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACGTGTGTTG‑GTTTAGCGCAACATGACCATAGTTTGACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttt                             <  1:671992/148‑1 (MQ=255)

                                                                                                       ggACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttt                             <  1:973535/148‑1 (MQ=255)

                                                                                                       ggACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAAAGACGATAGAGTCAGTATTAACTCAttt                             <  2:459964/148‑1 (MQ=255)

                                                                                                         aCAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttttt                           <  2:242739/148‑1 (MQ=255)

                                                                                                              gCCGAAGCGTGAGCATAGTTTTTACCATACATG‑GTTGTATGTACCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCCTACCCAATGATCCTGAAATGACGATAGAGTCAGCATTAACTTATTTTTCAATa                      <  1:701047/148‑1 (MQ=255)

                                                                                                              gCCGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATa                      >  1:247958/1‑148 (MQ=255)

                                                                                                               ccGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAg                     <  2:935107/148‑1 (MQ=255)

                                                                                                               ccGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAg                     >  1:942409/1‑148 (MQ=255)

                                                                                                                cGACGCGTGAGCAAAGTCTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGAGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGa                    <  2:665604/148‑1 (MQ=255)

                                                                                                                cGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTACAGGCTTACCCAATGCTCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGa                    <  2:89394/148‑1 (MQ=255)

                                                                                                                cGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGa                    <  1:181554/148‑1 (MQ=255)

                                                                                                                cGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGa                    <  1:746552/148‑1 (MQ=255)

                                                                                                                cGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGa                    >  1:812624/1‑148 (MQ=255)

                                                                                                                cGAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGGGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGGCAGTATTAACTCATTTTTCAATAGa                    >  1:406315/1‑148 (MQ=255)

                                                                                                                cGAAGCGGGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGa                    <  1:220487/148‑1 (MQ=255)

                                                                                                                 gAAGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGAt                   <  1:129838/148‑1 (MQ=255)

                                                                                                                  aaGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATc                  <  1:250401/148‑1 (MQ=255)

                                                                                                                  aaGCGTAAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCCCAACATGACCATAGTTTTACTAAAGCACAGCGCTAAGTGATTAAAGGCTGACCCAATTATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATc                  <  1:147694/148‑1 (MQ=255)

                                                                                                                   aGCGTGAGCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCa                 <  1:646698/148‑1 (MQ=255)

                                                                                                                         ggCAATGTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTCAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGa           <  1:873040/147‑1 (MQ=255)

                                                                                                                          gCAAAGTTTTTACCATACATG‑GTTGCATTTAGCGCAAAATGACCATAGTTTTACTAAAGAACAGAGCCAAGTGATTAAAGGCGTAGCCAATGAACCGGAAATGACAATCGAGGCCGAATTTAATCATGTTTGAATAGATCAggcgaac          >  2:8138/1‑143 (MQ=255)

                                                                                                                           cAAAGTTTTTACCATACATG‑GTTGCATTTAGCCCAACATGACCATAGTTTTACTAAAGCACAGCGCCAACTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACt         <  1:203471/148‑1 (MQ=255)

                                                                                                                            aaaGTTTTCACCATACATG‑GTTGCATTGAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTa        <  1:298393/148‑1 (MQ=255)

                                                                                                                               gTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCa     <  1:678274/148‑1 (MQ=255)

                                                                                                                               gTTTTTACCATACATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCa     <  1:426654/148‑1 (MQ=255)

                                                                                                                                tactgaCCATACCTG‑GTTGCTGTCAGCGCAGCATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCaa    >  2:709598/6‑148 (MQ=255)

                                                                                                                                 ttttACCAGACATG‑GTTGAATTAAGCTTAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCAAg   <  1:918648/148‑1 (MQ=255)

AGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATG‑GTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCAAGC  >  USA300TCH1516_ALE/479415‑479692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`