| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,664,466 | G→A | 50.4% | A65T (GCG→ACG) | pitA_2 → | L‑methionine sulfoximine/L‑methionine sulfone acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,664,466 | 0 | G | A | 50.4% | 0.1 / 17.2 | 19 | A65T (GCG→ACG) | pitA_2 | L‑methionine sulfoximine/L‑methionine sulfone acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (4/5); new base A (4/5); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGT > USA300TCH1516_ALE/2664331‑2664606 | gCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTacgacg < 1:275649/141‑1 (MQ=255) aTTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTc > 2:38060/1‑141 (MQ=255) aTCAATACGACAGCTATTTATACTTATGAACCGCAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCGTAGGGTTTGCGACGTTCGGt < 1:175620/141‑1 (MQ=255) aGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGAcc < 2:134988/141‑1 (MQ=255) gCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCt < 1:168074/141‑1 (MQ=255) cTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCtt > 1:46618/1‑141 (MQ=255) aaCCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCtat < 1:201412/141‑1 (MQ=255) tAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCGTTTATGCCTTGGCCGGCATCCCTATATACAATCGaaa > 1:6666/1‑140 (MQ=255) aGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTAGTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTCTATACAATCGAACa > 2:32656/1‑141 (MQ=255) aCGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTAt < 1:18284/141‑1 (MQ=255) gttGAAAGCAAACAACTGAAGCATGAGCCTATCCTTGTATGTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTGCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCt < 2:240905/140‑1 (MQ=255) aaacAACGTAAGCATGAGCCTTTCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTaga < 2:219531/141‑1 (MQ=255) aacGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGaa > 1:87347/1‑141 (MQ=255) gCATGGGCCTATCTTTGGATTTGAGGAAAATGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCCTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTAt < 2:144765/141‑1 (MQ=255) aTGAGCCTATCTTTTTATTTGAGGAAAAGGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTACATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTg < 2:81205/141‑1 (MQ=255) cTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTc < 1:38060/141‑1 (MQ=255) gAAAATGGAAATGCCTTAGAGTTTACGACTTTCGGTTCATTTAGACCTTTTCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTa > 2:205816/1‑141 (MQ=255) aaTGGAAGTGTCTTAGGGTTTACGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAAtt > 1:123273/1‑141 (MQ=255) aGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGaa > 1:236211/1‑141 (MQ=255) cTTAGGGTTTTGGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCaaa < 2:262332/141‑1 (MQ=255) gCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGt < 1:210681/141‑1 (MQ=255) | GCCATTATCAATACTACAGCTGTTTATACTTATGAACCACAAACCATAGACGAACGTGTCGCATGGTTTGAAACGAAACAACGTAAGCATGAGCCTATCTTTGTATTTGAGGAAAATGGAAGTGTCTTAGGGTTTGCGACGTTCGGTTCATTTAGACCTTGGCCAGCATACCTATATACAATCGAACATTCTATTTATGTCGATGCTTCAGCTAGAGGAAAAGGTATTGCTAGTCAATTACTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGT > USA300TCH1516_ALE/2664331‑2664606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |