Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,834,119	G→T	intergenic (+68/‑46)	osmC → / → USA300HOU_RS09140	Peroxiredoxin OsmC/Soluble hydrogenase 42 kDa subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,834,119	0	G	T	100.0%	67.6 / NA	20	intergenic (+68/‑46)	osmC/USA300HOU_RS09140	Peroxiredoxin OsmC/Soluble hydrogenase 42 kDa subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/11);  total (9/11)

TAATAATTGCATGATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAA  >  USA300TCH1516_ALE/1833976‑1834233                                                                                                                                                |                                                                                                                   taataaTTGCATGATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATaaaa                                                                                                                <  2:6246/148‑1 (MQ=255) taataaTTGCATGATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATaaaa                                                                                                                <  2:56439/148‑1 (MQ=255) taataaTTGCATGATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATaaaa                                                                                                                <  2:44419/148‑1 (MQ=255) taataaTTGCATGATTTCAAATGCTGTAAGAAATAATGTGAACATAATAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCAACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATaaaa                                                                                                                <  2:41650/148‑1 (MQ=255)   ataatTGCATGATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAAtt                                                                                                              <  1:90178/148‑1 (MQ=255)           aTGATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTaaca                                                                                                      >  2:206608/1‑146 (MQ=255)           aTGATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTaaaa                                                                                                      <  1:68695/148‑1 (MQ=255)               tttCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGt                                                                                                  <  2:205250/148‑1 (MQ=255)                        cTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCtta                                                                                         <  2:157967/148‑1 (MQ=255)                        cTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCAATCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCtta                                                                                         <  1:149882/148‑1 (MQ=255)                         tGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCttat                                                                                        <  2:231715/148‑1 (MQ=255)                         tGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCttat                                                                                        <  1:140795/148‑1 (MQ=255)                                       tgGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCata                                                                          >  2:134135/1‑148 (MQ=255)                                                                      cAAATAAAATCGTTAAGTGATAACGTTGGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTaa                                           >  2:222332/1‑148 (MQ=255)                                                                                                   ttCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTTAAATCTTGTTTAGAGGAGTCATATAAATTTATGATCATCAACCGGTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGcc              >  1:324410/1‑148 (MQ=255)                                                                                                        cAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTATCACCTGGCCCAACCCCTGTACCTGATGCCATTAt         >  1:354710/1‑148 (MQ=255)                                                                                                        cAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTAt         >  1:101671/1‑148 (MQ=255)                                                                                                        cAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTAt         >  1:126236/1‑148 (MQ=255)                                                                                                            ataGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATgaga     >  2:44780/1‑148 (MQ=255)                                                                                                               gCGTTATCACTTTTTTTTTTTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGaa  >  2:150767/1‑148 (MQ=255)                                                                                                                                                |                                                                                                                   TAATAATTGCATGATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAA  >  USA300TCH1516_ALE/1833976‑1834233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑