| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,238,307 | C→T | 100% | Q141* (CAA→TAA) | USA300HOU_RS06160 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,238,307 | 0 | C | T | 100.0% | 19.6 / NA | 8 | Q141* (CAA→TAA) | USA300HOU_RS06160 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (2/6); total (2/6) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGCAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGA > USA300TCH1516_ALE/1238170‑1238425 | gTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAg > 1:130861/1‑141 (MQ=255) tGAAAGTGAATATGAAATTAATTCAAAATTGTTAGCGGAAAGATTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGa < 2:132287/141‑1 (MQ=255) gAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGaa < 1:101069/141‑1 (MQ=255) cAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACatt < 2:130861/141‑1 (MQ=255) ttGAAACGAAATAAAAAGCTGTTATGAAACCAGTTGAAGGGACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAg < 2:63797/141‑1 (MQ=255) aTGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAacaca > 1:84718/1‑141 (MQ=255) ttGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACttat < 1:8900/141‑1 (MQ=255) tACAGGTGCGAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAaaga < 1:86725/141‑1 (MQ=255) | GTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGCAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGA > USA300TCH1516_ALE/1238170‑1238425 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |