| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,770,911 | T→A | 100% | S73C (AGT→TGT) | USA300HOU_RS08840 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,770,911 | 0 | T | A | 100.0% | 44.1 / NA | 13 | S73C (AGT→TGT) | USA300HOU_RS08840 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (6/7); total (6/7) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCACTCGAAGGCAAAAGTATTTTTTGGTTAGTTTGATTAAATGATTTTAAAAATCCTTCTTGAGAAAAGTCGTTTGGCATAAAATCAACTCGAATGCCAAGTGATTCACAATATTGCGCTGTCTTACTTCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTCACAGCATTTTTAGATGAAAAAATAAGCCAGTCATAGCGTTGATTTAACAAATGAATATCAAAATTTAGTGGCTTTATA > USA300TCH1516_ALE/1770783‑1771047 | ttCACTCGAAGGCAAAAGTATTTTTTGGTTAGTTTGATTAAATGATTTTAAAAATCCTTCTTGAGAAAAGTCGTTTGGCATAAAATCAACTCGACTGCCAAGTGATTCACAATATTGCGCTCTCTTACATCCTATCACAGc > 1:4905/1‑141 (MQ=255) aGTATTTTTTGGTTAGTTTGATTAAATGATTTTAAAAATCCTTCTTGAGAAAAGTCGTTTGGCATAAAATCAACTCGAATGCCAAGTGATTCACAATATTGCGCTGTCTTACATCCTATCACAGCAATGTTATCAACATTa > 1:58599/1‑141 (MQ=255) aTTAAATGATTTTAAAAATCCTTCTTGAGAAAAGTCGTTTGGCATAAAATCAACTCGAATGCCAAGTGATTCACAATATTGCGCTGTCTTACATCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTtata > 1:61843/1‑141 (MQ=255) aaaaaTCCTTCTTGAGAAAAGTCGTTTGGCATAAAATCAACTCGAATGCCAAGTGATTCACAATATTGCGCTGTCTTACATCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTcaca > 1:107982/1‑141 (MQ=255) aaaTCCTTCTTGAGAAAAGTCGTTTGGCATAAAATCAACTCGAATGCCAAGTGATTCACAATATTGCGCTGTCTTACATCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTCACAGc < 1:64379/141‑1 (MQ=255) gaAAAGTCGTTTGGCATAAAATCAACTCGAATGCCAAGTGATTCACAATATTGCGCTGTCTTACATCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTCACAGCATTTTTAGATGaa < 1:21044/141‑1 (MQ=255) gTCGTTTGGCATAAAATCAACTCTAATGCCAAGTGATTCACAATATTGCGCTGTCTTACATCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTCACAGCATTTTTAGATGAAAAAAt < 2:114877/141‑1 (MQ=255) gTCGTTTGGCATAAAATCAACTCGAATGCCAAGTGATTCACAATATTGCGCTGTCTTACATCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTCACAGCATTTTTAGATGAAAAAAt < 2:114872/141‑1 (MQ=255) cGTTTGGCATAAAATCAACTCGAATGCCAAGTGATTCACAATATTGCGCTGTCTTACATCCTATCACATCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTCACAGCATTTTTAGATGAAAAAATaa < 1:31090/141‑1 (MQ=255) aaaaTCAACTCGAATGCCAAGTGATTCACAATATTGCGCTGTCTTACATCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTCACAGCATTTTTAGATGAAAAAATAAGCCAGTCATa < 1:23292/141‑1 (MQ=255) acaATATTGCGCTGTCTTACATCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTCACAGCATTTTTAGATGAAAAAATAAGCCAGTCATAGCGTTGATTTAACAAATGAATATCaaa > 1:76394/1‑141 (MQ=255) atatTGCGCTGTCTTACATCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTCACAGCATTTTTAGATGAAAAAATAAGCCAGTCATAGCGTTGATTTAACAAATGAATATCAAAAtt < 2:62392/141‑1 (MQ=255) ttACATCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTCACAGCATTTTTAGATGAAAAAATAAGCCAGTCATAGCGGTGATTTAACAAATGAATATCAAAATTTAGTGGCTTtata > 1:102590/1‑141 (MQ=255) | TTCACTCGAAGGCAAAAGTATTTTTTGGTTAGTTTGATTAAATGATTTTAAAAATCCTTCTTGAGAAAAGTCGTTTGGCATAAAATCAACTCGAATGCCAAGTGATTCACAATATTGCGCTGTCTTACTTCCTATCACAGCAATGTTATCAACATTAATTCCTTTTAGATATTTATAAAAGAATTTCACAGCATTTTTAGATGAAAAAATAAGCCAGTCATAGCGTTGATTTAACAAATGAATATCAAAATTTAGTGGCTTTATA > USA300TCH1516_ALE/1770783‑1771047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |