| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,883,654 | C→T | 100% | G522D (GGT→GAT) | ebh_2 ← | Extracellular matrix‑binding protein ebh |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,883,654 | 0 | C | T | 100.0% | 52.6 / NA | 17 | G522D (GGT→GAT) | ebh_2 | Extracellular matrix‑binding protein ebh |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (8/9); total (8/9) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATCTTGTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTACCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATT > USA300TCH1516_ALE/1883517‑1883777 | aaTCTTGTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTAtctc > 1:57651/1‑141 (MQ=255) tGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACtttt < 1:94015/141‑1 (MQ=255) tttATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAAt < 2:86255/141‑1 (MQ=255) tttATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAAt < 2:85839/141‑1 (MQ=255) tttATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAAt < 2:12826/141‑1 (MQ=255) gTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATc > 2:110694/1‑141 (MQ=255) tcGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGAttt < 1:98439/141‑1 (MQ=255) aaTGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGca > 2:12828/1‑141 (MQ=255) aaTGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGca > 1:99276/1‑141 (MQ=255) tGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGcatc > 2:84516/1‑141 (MQ=255) gTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTAAATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGcatca > 1:119637/1‑141 (MQ=255) aTTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAAt < 1:87288/141‑1 (MQ=255) tttATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAAtt < 2:5472/141‑1 (MQ=255) aTCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGaaa < 2:59728/141‑1 (MQ=255) aTCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGaaa < 1:121765/141‑1 (MQ=255) cGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAAt > 2:138652/1‑141 (MQ=255) cGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAAt > 2:76515/1‑141 (MQ=255) ccGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATAtt > 2:75491/1‑141 (MQ=255) | AATCTTGTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTACCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATT > USA300TCH1516_ALE/1883517‑1883777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |