| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,388,866 | A→G | 100% | intergenic (‑149/+542) | sarV ← / ← moaA | HTH‑type transcriptional regulator SarV/GTP 3',8‑cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,388,866 | 0 | A | G | 100.0% | 55.0 / NA | 16 | intergenic (‑149/+542) | sarV/moaA | HTH‑type transcriptional regulator SarV/GTP 3',8‑cyclase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/10); total (6/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGACAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGAAATGA > USA300TCH1516_ALE/2388740‑2389002 | ttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTa < 2:41816/141‑1 (MQ=255) aTGTTTGGAATTCGTTTTATCATACTAATACGTAAATGTAATAGATATCAGACTGAGCATTGTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGaca < 1:47820/141‑1 (MQ=255) tGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGGTTATTCGACAACAtt > 1:115337/1‑141 (MQ=255) aTTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCCTTCTATCAATTATAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTtata < 1:8681/141‑1 (MQ=255) aTTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTtata < 2:133538/141‑1 (MQ=255) ttCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATAc < 2:65842/141‑1 (MQ=255) attatACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATGAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTaaaaaa > 1:27566/1‑141 (MQ=255) tatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAg < 2:111254/141‑1 (MQ=255) aaCTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGa < 1:114132/141‑1 (MQ=255) aCTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGAc < 2:73209/141‑1 (MQ=255) tCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAg > 2:127425/1‑141 (MQ=255) aaTATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTaa > 1:10476/1‑141 (MQ=255) aTTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATttat > 2:108727/1‑141 (MQ=255) atatCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGa < 2:50146/141‑1 (MQ=255) ttGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCtt < 2:128257/141‑1 (MQ=255) ttGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGAAATGa > 2:63391/1‑141 (MQ=255) | TTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGACAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGAAATGA > USA300TCH1516_ALE/2388740‑2389002 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |