BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,709,600	(T)_5→4	coding (1327/1812 nt)	oatA_2 ←	O‑acetyltransferase OatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,709,596	0	T	.	99.1%	534.7 / ‑1.7	111	coding (1331/1812 nt)	oatA_2	O‑acetyltransferase OatA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (48/62); minor base C (0/1); total (48/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 123 total aligned reads displayed.

TATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCAAA  >  USA300TCH1516_ALE/2709456‑2709732

tataTTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACt                                                                                                                                           <  1:217764/141‑1 (MQ=255)

tataTTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACt                                                                                                                                           <  4:331207/141‑1 (MQ=255)

  tatTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttt                                                                                                                                         >  1:813315/1‑141 (MQ=255)

   atTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACtttt                                                                                                                                        >  2:655649/1‑141 (MQ=255)

   atTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACtttt                                                                                                                                        >  2:49764/1‑141 (MQ=255)

     tGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaa                                                                                                                                      >  1:208807/1‑139 (MQ=255)

     tGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaa                                                                                                                                      <  3:328741/141‑3 (MQ=255)

     tGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaa                                                                                                                                      <  1:352128/141‑3 (MQ=255)

     tGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaa                                                                                                                                      >  3:338984/1‑139 (MQ=255)

       cGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaata                                                                                                                                    <  3:287700/141‑5 (MQ=255)

                 aTTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGc                                                                                                                         >  1:123047/1‑141 (MQ=255)

                 aTTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGc                                                                                                                         >  3:217578/1‑141 (MQ=255)

                  ttGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCt                                                                                                                        <  2:119678/141‑1 (MQ=255)

                  ttGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCt                                                                                                                        <  2:454149/141‑1 (MQ=255)

                   tGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTa                                                                                                                       >  3:144142/1‑141 (MQ=255)

                   tGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTa                                                                                                                       >  2:539293/1‑141 (MQ=255)

                      tgtAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTc                                                                                                                    <  4:203136/141‑1 (MQ=255)

                      tgtAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTc                                                                                                                    <  2:123047/141‑1 (MQ=255)

                       gtAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCg                                                                                                                   >  2:597707/1‑141 (MQ=255)

                          gCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTg                                                                                                                >  2:111644/1‑141 (MQ=255)

                           cATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGt                                                                                                               <  1:444316/141‑1 (MQ=255)

                           cATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGt                                                                                                               <  2:73194/141‑1 (MQ=255)

                              cAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTg                                                                                                            >  2:430946/1‑141 (MQ=255)

                                aCGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCt                                                                                                          <  1:691488/141‑1 (MQ=255)

                                  gAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTa                                                                                                        <  2:710024/141‑1 (MQ=255)

                                   aGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTGTAATATCCTCTTTGCTATTCGCTGTCTGCTTAt                                                                                                       <  4:266089/141‑1 (MQ=255)

                                    gTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATc                                                                                                      >  4:41719/1‑141 (MQ=255)

                                     ttGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATcc                                                                                                     >  1:92935/1‑141 (MQ=255)

                                       gCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCctc                                                                                                   >  2:956751/1‑141 (MQ=255)

                                       gCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCctc                                                                                                   >  1:775183/1‑141 (MQ=255)

                                          gTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTg                                                                                                <  3:275876/141‑1 (MQ=255)

                                           tCCATCTTTACCCTCAATTTGTGCATTTGGTATTTTCTTAGTAACGCCAATACCAATATCCACCATGACCGAGTCACCAATTAATAGTCGTGATG‑CCTTTTAATATCCTCTTTGCTATTCGCTGTCTGCGTATCCTCTTGc                                                                                               <  2:449298/141‑1 (MQ=255)

                                            ccAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCt                                                                                              <  1:637101/141‑1 (MQ=255)

                                            ccAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCt                                                                                              <  2:207644/141‑1 (MQ=255)

                                              aaCTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTa                                                                                            >  3:158799/1‑141 (MQ=255)

                                                    gccATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCtttt                                                                                      <  1:636044/140‑1 (MQ=255)

                                                       aTCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTa                                                                                   >  1:174082/1‑141 (MQ=255)

                                                       aTCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTa                                                                                   >  3:126349/1‑141 (MQ=255)

                                                       aTCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTa                                                                                   <  1:623601/141‑1 (MQ=255)

                                                        tCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTaa                                                                                  >  1:4746/1‑141 (MQ=255)

                                                        tCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTaa                                                                                  >  3:273906/1‑141 (MQ=255)

                                                         cAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAAc                                                                                 <  1:450477/141‑1 (MQ=255)

                                                          aaTTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACg                                                                                <  3:407614/141‑1 (MQ=255)

                                                            tttGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGAc                                                                              <  1:61618/141‑1 (MQ=255)

                                                             ttGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCCATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACt                                                                             <  1:262290/141‑1 (MQ=255)

                                                             ttGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACt                                                                             <  2:365377/141‑1 (MQ=255)

                                                                 gCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACttttt                                                                         >  2:285363/1‑141 (MQ=255)

                                                                   aTTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTttc                                                                       <  4:223633/141‑1 (MQ=255)

                                                                   aTTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTttc                                                                       <  1:872888/141‑1 (MQ=255)

                                                                     ttGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTttctt                                                                     <  2:766672/141‑1 (MQ=255)

                                                                        ttATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCgt                                                                  <  1:947544/140‑1 (MQ=255)

                                                                        gTATTTTCTTAGTAAAGACATGACCAATATCCACCATGACCGATTCACCATTTAAGAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGTCTTTTttcttctt                                                                  >  4:246625/1‑139 (MQ=255)

                                                                         tATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCgtt                                                                 <  2:237064/141‑1 (MQ=255)

                                                                             ttCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑ttt                                                            >  3:373682/1‑141 (MQ=255)

                                                                              tCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑tttt                                                           >  2:714206/1‑141 (MQ=255)

                                                                              tCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑tttt                                                           >  1:284453/1‑141 (MQ=255)

                                                                                 tAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTaaa                                                        >  3:287765/1‑141 (MQ=255)

                                                                                 tAGTAAAGACATTACCAATATCCACCATGACCGAGACACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCCTATCTTTTTTCACGACTTTTTTCTTCGTTG‑TTTTaaa                                                        >  2:802854/1‑141 (MQ=255)

                                                                                  aGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAAt                                                       <  1:430946/141‑1 (MQ=255)

                                                                                        gACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGtc                                                 >  1:415798/1‑141 (MQ=255)

                                                                                         aCATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGtct                                                >  2:672719/1‑141 (MQ=255)

                                                                                           aTTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGtcttc                                              <  1:538693/141‑1 (MQ=255)

                                                                                            ttACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGtcttct                                             >  2:642304/1‑141 (MQ=255)

                                                                                             tACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGtcttctt                                            <  4:239267/141‑1 (MQ=255)

                                                                                             tACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTCTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGtcttctt                                            >  4:390011/1‑141 (MQ=255)

                                                                                                cAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTtctc                                         <  2:130076/141‑1 (MQ=255)

                                                                                                   tatCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCtcttt                                      <  3:408834/141‑1 (MQ=255)

                                                                                                     tCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCtctttct                                    >  2:838993/1‑141 (MQ=255)

                                                                                                       caccaTGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTttcttc                                  >  2:485992/1‑141 (MQ=255)

                                                                                                         ccaTGACCGAGTCACCAATTAATAGAGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAATGTCTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTtctc                                >  2:244117/1‑141 (MQ=255)

                                                                                                           aTGACCGAGTCGCCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCgg                              <  2:496546/141‑1 (MQ=255)

                                                                                                              aCCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCtt                           <  1:315535/141‑1 (MQ=255)

                                                                                                              aCCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCGTCGTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCtt                           <  1:140477/141‑1 (MQ=255)

                                                                                                                  aGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtg                       <  1:774008/141‑1 (MQ=255)

                                                                                                                  aGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCCTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtg                       <  4:7096/141‑1 (MQ=255)

                                                                                                                   gTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATc                                                                                          >  1:446130/1‑74 (MQ=255)

                                                                                                                   gTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATc                                                                                          <  2:446130/74‑1 (MQ=255)

                                                                                                                   gTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgt                      >  2:316421/1‑141 (MQ=255)

                                                                                                                   gTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgt                      >  2:449642/1‑141 (MQ=255)

                                                                                                                     cACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgttg                    <  2:918659/141‑1 (MQ=255)

                                                                                                                     cACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgttg                    <  1:233262/141‑1 (MQ=255)

                                                                                                                      aCCAATTAATAGTTGTGATGAC‑TTTTAATAGCCTCTTTGCTAGTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgttgt                   <  2:789180/141‑1 (MQ=255)

                                                                                                                      aCCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgttgt                   <  2:92935/141‑1 (MQ=255)

                                                                                                                       ccAATTAATGGTGGTGATGAC‑TTTTAATATCCTCTTGGCTATTCGCTGTCTGCTTATCCTCTTGCTTATC‑TTTTTATCGCCTTTTTTCTTCGTTG‑TTTTTATTTCCCTCTTCTTCTCTTTCTTCTCGGCTTCAtgttgtgt                 <  2:339837/141‑3 (MQ=255)

                                                                                                                        cAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGttt                 <  2:161413/141‑1 (MQ=255)

                                                                                                                             aaTAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCaa            <  2:776849/141‑1 (MQ=255)

                                                                                                                              aTAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCtt                                                                                             <  1:539693/60‑1 (MQ=255)

                                                                                                                              aTAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAg           <  2:775183/141‑1 (MQ=255)

                                                                                                                              aTAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAg           <  1:517704/141‑1 (MQ=255)

                                                                                                                               tAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGt          >  2:59388/1‑141 (MQ=255)

                                                                                                                               tAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGt          >  3:130838/1‑141 (MQ=255)

                                                                                                                               tAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGt          >  3:275895/1‑141 (MQ=255)

                                                                                                                                aGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTg         <  1:298392/141‑1 (MQ=255)

                                                                                                                                aGTGGTGATGAC‑TTTTAACATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTg         >  1:599475/1‑141 (MQ=255)

                                                                                                                                 gtggtgATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTGAAACGAGTTTTGGGTT‑GTTGTTTTTTAATTTCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTGGTTTGCCAAGTGc        >  1:335303/1‑141 (MQ=255)

                                                                                                                                  tggtgATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGCCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCa       >  1:762165/1‑141 (MQ=255)

                                                                                                                                   ggtgATGNC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCAt      <  2:727404/141‑1 (MQ=255)

                                                                                                                                   ggtgATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCAt      <  4:151019/141‑1 (MQ=255)

                                                                                                                                     tgatgaC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCa    <  2:343734/141‑1 (MQ=255)

                                                                                                                                       atgaC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCaaa  <  1:397601/141‑1 (MQ=255)

TATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTG‑TTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCATCAAA  >  USA300TCH1516_ALE/2709456‑2709732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`