BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,018,733	(A)_6→7	coding (186/810 nt)	mgt ←	Monofunctional glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,018,727	1	.	A	100.0%	393.6 / NA	110	L64F (TTA→TTT)	mgt	Monofunctional glycosyltransferase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (41/69); total (41/69)

Only 100 of 128 total aligned reads displayed.

TCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑AAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGT  >  USA300TCH1516_ALE/2018587‑2018859

tCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAt                                                                                                                                       <  2:72645/141‑1 (MQ=255)

 cGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCTTTTTTTTTTTGTTCATCCACATTATCTCGTGTAGAT‑a                                                                                                                                     >  4:271203/1‑141 (MQ=255)

 cGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑a                                                                                                                                     >  1:863860/1‑141 (MQ=255)

  gAATCCATGATGATTGTAGAATCGTTCCTCTTCCATTGAAATAAAGGCACCTTTAACATTCTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aa                                                                                                                                    <  2:828477/141‑1 (MQ=255)

  gAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAACGGCACATTTAACATCCTCTAGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aa                                                                                                                                    <  1:484119/141‑1 (MQ=255)

  gAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aa                                                                                                                                    <  2:81777/141‑1 (MQ=255)

  gAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aa                                                                                                                                    >  1:48162/1‑141 (MQ=255)

  gAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTATAGAT‑aa                                                                                                                                    <  2:773585/141‑1 (MQ=255)

   aaTCCATGATGATTGTAGAATCGTTCATCTTCCATTTAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaa                                                                                                                                   <  2:494293/141‑1 (MQ=255)

   aaTCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaa                                                                                                                                   <  3:136910/141‑1 (MQ=255)

     tCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaaaa                                                                                                                                 <  1:235719/141‑1 (MQ=255)

       cATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaaaaaa                                                                                                                               <  1:700298/141‑2 (MQ=255)

         tgatgaTTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaaaaaata                                                                                                                             >  1:111931/1‑138 (MQ=255)

          gatgatTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaaaaaatac                                                                                                                            <  1:383530/141‑5 (MQ=255)

           atgatTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaaaaaataca                                                                                                                           >  1:62817/1‑136 (MQ=255)

               ttGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGAt                                                                                                                        >  1:827999/1‑141 (MQ=255)

                 gTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATATTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATAc                                                                                                                      >  3:298098/1‑141 (MQ=255)

                   aGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCa                                                                                                                    >  2:895091/1‑141 (MQ=255)

                     caTCGTTCATCTTCCACTGACATCAAGGCACCTTTAACATACTATGGCCTGTTATCAGCTGCCACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAAt                                                                                                                  <  4:86652/140‑1 (MQ=255)

                     aaTCGTTAATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGAAAAAAAAAAAAAAAATACATGATACCAAt                                                                                                                  <  2:480416/141‑1 (MQ=255)

                      aTCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATa                                                                                                                 >  1:946867/1‑141 (MQ=255)

                             aTCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATg                                                                                                          <  2:478842/141‑1 (MQ=255)

                               cTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCg                                                                                                        >  2:859507/1‑141 (MQ=255)

                                ttCCATTGAAATAAAGGCACCTTTAACATCCTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGa                                                                                                       <  3:413861/141‑1 (MQ=255)

                                  ccATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAAAAAAACGATa                                                                                                     >  2:619988/1‑141 (MQ=255)

                                   cATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATaa                                                                                                    <  1:415860/141‑1 (MQ=255)

                                     ttGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATg                                                                                                  <  1:149300/141‑1 (MQ=255)

                                       gAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATTCCAATAAACAATGCGATAATGAt                                                                                                <  1:867483/141‑1 (MQ=255)

                                       aataTAAAGACACCTTTAACATTCTCTGGCATGTTATCAGCTAACAAAAAACTACTTTTATTTTAAATTTTTCTTAGTTCATCCACAATATCGCGTGTAGATAAAAAAATACATGATACCAATAAACTATGCGATAATGAt                                                                                                <  2:360133/138‑1 (MQ=255)

                                       aaaaTAAAGGAAGCTTTAAGATACTTTGGCATGATATCAGCAGACACAAAACTACTTTTATTTTAAATTTTTCTTAGTTCATCCACATTATAAAAAAAAAAAAAAAAAATACATGATACCAATAAACAATGCGATAATGAt                                                                                                <  4:300622/140‑1 (MQ=255)

                                        aaaTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGAtt                                                                                               >  2:692355/1‑141 (MQ=255)

                                        aaaTAAAGCCACATTTAACATACTCTGGCATGTTATCAGCTGACAAAAAACTACTTTTATTTCAAATTTTTCTTGGTTCATCCACATTATCGCGAATAAATAAAAAAATACATGATACCAATAAACAATGCGATAATGAtt                                                                                               <  2:512494/141‑1 (MQ=255)

                                         aaTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTa                                                                                              >  2:679310/1‑141 (MQ=255)

                                          aTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAg                                                                                             >  4:227015/1‑141 (MQ=255)

                                          aTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAg                                                                                             <  3:54279/141‑1 (MQ=255)

                                           tAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGa                                                                                            >  1:631510/1‑141 (MQ=255)

                                            aaaGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGaa                                                                                           <  1:893081/141‑1 (MQ=255)

                                            aaaGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGaa                                                                                           <  1:469138/141‑1 (MQ=255)

                                             aaGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAAt                                                                                          >  3:110315/1‑141 (MQ=255)

                                             aaGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAAt                                                                                          >  1:820401/1‑141 (MQ=255)

                                               ggCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATgg                                                                                        >  1:846664/1‑141 (MQ=255)

                                                   ccTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGCTTAGAATGGTtaa                                                                                    >  3:353236/1‑141 (MQ=255)

                                                     tttAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTtaata                                                                                  <  2:794826/141‑1 (MQ=255)

                                                      ttAACAGACTCTGGCATGTTATCAGCTGACACAAAACTACTTGTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTtaataa                                                                                 <  2:923344/141‑1 (MQ=255)

                                                        aaCATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAataata                                                                               <  1:318462/141‑1 (MQ=255)

                                                           aTACTCTGACATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATAttt                                                                            <  2:21576/141‑1 (MQ=255)

                                                              ctctGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTa                                                                         >  2:27843/1‑141 (MQ=255)

                                                              ctctGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTa                                                                         <  3:180664/141‑1 (MQ=255)

                                                                    cATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTa                                                                   >  1:811216/1‑141 (MQ=255)

                                                                       gTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTc                                                                <  3:302610/141‑1 (MQ=255)

                                                                        ttATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTct                                                               >  4:9868/1‑140 (MQ=255)

                                                                        ttATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCg                                                               >  2:242768/1‑141 (MQ=255)

                                                                        ttATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCg                                                               >  3:169147/1‑141 (MQ=255)

                                                                         tATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGt                                                              <  1:741761/141‑1 (MQ=255)

                                                                          aTCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTtaataa                                                                                 >  4:113698/1‑121 (MQ=255)

                                                                          aTCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGtt                                                             >  2:419300/1‑141 (MQ=255)

                                                                          aTCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGtt                                                             <  1:608428/141‑1 (MQ=255)

                                                                           tCAGCTGACACAAGACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGttt                                                            <  4:5340/141‑1 (MQ=255)

                                                                             aGCAGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGAGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAc                                                          <  2:750716/141‑1 (MQ=255)

                                                                                tGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACttt                                                       <  2:546180/141‑1 (MQ=255)

                                                                                tGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACttt                                                       >  1:6570/1‑141 (MQ=255)

                                                                                  acacAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACttttt                                                     >  4:330803/1‑141 (MQ=255)

                                                                                  acacAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACttttt                                                     >  1:375937/1‑141 (MQ=255)

                                                                                   cacaAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGAAAAAAAAAAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACtttttt                                                    <  2:137512/141‑1 (MQ=255)

                                                                                     caAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTtttttc                                                  <  1:92678/141‑1 (MQ=255)

                                                                                     caAAACTACTTTTATTTTCAATTTTTATTAGTTCATCCACGTTATCGCGTGTAGATAAAAAAATACATTATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTtttttc                                                  <  2:960318/141‑1 (MQ=255)

                                                                                      aaaaCTACTTTTATTTTCAATTTTGCATAGTTCATCCAGATTAACGCGTGTAGATAAAAAAATACATGATAACAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTtttttct                                                 <  2:387803/141‑1 (MQ=255)

                                                                                       aaaCTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTtttttctt                                                >  3:207635/1‑141 (MQ=255)

                                                                                         actactTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTtttttctttt                                              <  3:138568/141‑1 (MQ=255)

                                                                                          ttactTTTATTTTCAATTTTTCTTATTTCATCCACATTATCGCTTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTTATAGTATTCGTTTACTTtttttcttgtt                                             <  4:350358/140‑1 (MQ=255)

                                                                                          ctactTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTtttttcttttt                                             <  1:813999/141‑1 (MQ=255)

                                                                                               tttATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTcggcg                                        <  2:946867/141‑1 (MQ=255)

                                                                                               tttATTTTCAATATTTCTTAGTTCATCCACATTATAGAAAAAAAAAAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTcggcg                                        <  2:311146/141‑1 (MQ=255)

                                                                                                ttATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTcggcgg                                       <  1:738789/141‑1 (MQ=255)

                                                                                                        aaTTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCaa                               <  1:607094/141‑1 (MQ=255)

                                                                                                         aTTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAAc                              <  2:634990/141‑1 (MQ=255)

                                                                                                             ttCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGt                          <  3:269805/141‑1 (MQ=255)

                                                                                                             ttCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGt                          <  3:73415/141‑1 (MQ=255)

                                                                                                               cTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTg                        <  1:265768/141‑1 (MQ=255)

                                                                                                                ttAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGa                       <  2:111931/141‑1 (MQ=255)

                                                                                                                 tAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGat                      <  3:11610/141‑1 (MQ=255)

                                                                                                                  aGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGata                     <  1:652883/141‑1 (MQ=255)

                                                                                                                        tCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGTCGGTTTGCCAACTGGTTGATAATACGt               >  2:876931/1‑141 (MQ=255)

                                                                                                                         ccACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGta              <  3:85992/141‑1 (MQ=255)

                                                                                                                          cacaTTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtat             <  4:110315/141‑1 (MQ=255)

                                                                                                                            caTTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtatta           >  3:239251/1‑141 (MQ=255)

                                                                                                                            caTTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtatta           <  3:156442/141‑1 (MQ=255)

                                                                                                                               tATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAg        <  2:692806/141‑1 (MQ=255)

                                                                                                                                aTCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGt       <  2:228149/141‑1 (MQ=255)

                                                                                                                                aTCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGt       <  2:748665/141‑1 (MQ=255)

                                                                                                                                aTCGCGTGTAAATAAAAAAATATATGATACCAATAAATGTTGGGATAATGTTTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTATTTTTTTTCTTTTTTCGCGGTTAGGCAACTATTTTATGATATGTATTTTAGt       <  4:186306/141‑1 (MQ=255)

                                                                                                                                  cgcgTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGa     <  4:146361/141‑1 (MQ=255)

                                                                                                                                  cgcgTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGa     <  1:156126/141‑1 (MQ=255)

                                                                                                                                  cgcgAGAAGATAAAAAAATACATGATTCTAATAAACCATACGATAATGATTAGAATGGCTAATAATATGTTTAATAGTATTCGTTTACTTTTTTCCTTTTTCGGTGGTTTGCCTGCGGGTTGATAATACGTATTATACTGa     <  2:238173/141‑1 (MQ=255)

                                                                                                                                    cgTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAgg   >  2:553013/1‑141 (MQ=255)

                                                                                                                                    cgTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAgg   >  2:826227/1‑141 (MQ=255)

                                                                                                                                     gtgtAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGt  <  1:731020/141‑1 (MQ=255)

                                                                                                                                     gtgtAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGt  <  1:395315/141‑1 (MQ=255)

                                                                                                                                     gtgtAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGt  <  1:324125/141‑1 (MQ=255)

                                                                                                                                     gtgtAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGt  <  1:134851/141‑1 (MQ=255)

TCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑AAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGT  >  USA300TCH1516_ALE/2018587‑2018859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`