BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,238,307	C→T	Q141* (CAA→TAA)	USA300HOU_RS06160 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,238,307	0	C	T	100.0%	417.6 / NA	117	Q141* (CAA→TAA)	USA300HOU_RS06160	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (55/62); total (55/62)

Only 100 of 117 total aligned reads displayed.

GTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGCAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTGTTGTTGATAG  >  USA300TCH1516_ALE/1238170‑1238443

gTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAg                                                                                                                                       >  1:796367/1‑141 (MQ=255)

gTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAg                                                                                                                                       >  2:842735/1‑141 (MQ=255)

gTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAg                                                                                                                                       >  2:640894/1‑141 (MQ=255)

 tAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGc                                                                                                                                      <  2:631127/141‑1 (MQ=255)

   aaaaTATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTg                                                                                                                                    >  1:931133/1‑141 (MQ=255)

   aaaaTATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTg                                                                                                                                    <  2:886576/141‑1 (MQ=255)

    aaaTATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGc                                                                                                                                   <  2:898680/141‑1 (MQ=255)

    aaaTATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTAAAGGTACAATACTTACAGTTGAAAAAGATGATGCGTAACCTGc                                                                                                                                   <  2:137708/141‑1 (MQ=255)

    aaaTATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTATAGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAgaaaa                                                                                                                                   <  4:348524/141‑5 (MQ=255)

        atTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATa                                                                                                                               >  2:738889/1‑141 (MQ=255)

        atTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATa                                                                                                                               >  2:156585/1‑141 (MQ=255)

         ttGAAAGTGAATCGGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAg                                                                                                                              <  4:138681/141‑1 (MQ=255)

          tGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGa                                                                                                                             <  3:157392/141‑1 (MQ=255)

           gAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCCGTTGAGGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGaa                                                                                                                            >  2:753975/1‑141 (MQ=255)

           gAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGaa                                                                                                                            >  1:662529/1‑141 (MQ=255)

               gTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAg                                                                                                                        <  1:888884/141‑1 (MQ=255)

                tGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGc                                                                                                                       <  2:151879/141‑1 (MQ=255)

                 gAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCa                                                                                                                      >  3:134897/1‑141 (MQ=255)

                    tCTGAAATTAATTCAAAAGTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAaat                                                                                                                   <  1:779442/141‑1 (MQ=255)

                     cTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAaata                                                                                                                  >  2:575139/1‑141 (MQ=255)

                      tGAAATTAATTCAAAATTGTAAGCTGAGAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAaataa                                                                                                                 <  2:921253/141‑1 (MQ=255)

                           ttaattCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTg                                                                                                            <  4:319012/141‑1 (MQ=255)

                             aattCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGaa                                                                                                          <  3:10576/141‑1 (MQ=255)

                             aattCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGaa                                                                                                          >  1:339430/1‑141 (MQ=255)

                                tCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGAt                                                                                                       <  2:460623/141‑1 (MQ=255)

                                acAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAACTAAAAAAAAAAAAGCAAATAATACTGAAGAt                                                                                                       <  2:197856/140‑1 (MQ=255)

                                 cAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGAtt                                                                                                      >  1:932151/1‑141 (MQ=255)

                                  aaaaTTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTg                                                                                                     <  4:410327/141‑1 (MQ=255)

                                     aTTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGtat                                                                                                  <  1:380509/141‑1 (MQ=255)

                                      ttGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGtata                                                                                                 <  1:212884/141‑1 (MQ=255)

                                        gTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACGGAACATTGTATAGa                                                                                               >  4:17132/1‑141 (MQ=255)

                                         ttAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGaa                                                                                              >  1:412616/1‑141 (MQ=255)

                                           aGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGtatatagac                                                                                            >  3:125158/1‑136 (MQ=255)

                                           aGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAAtt                                                                                            >  2:303227/1‑141 (MQ=255)

                                           aGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAAtt                                                                                            >  4:336836/1‑141 (MQ=255)

                                                aaaGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATgg                                                                                       <  3:262777/141‑1 (MQ=255)

                                                  aGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATTATACTGAAGATTGTATAGAATTAATGGAg                                                                                     <  2:416133/141‑1 (MQ=255)

                                                  aGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAg                                                                                     <  4:213468/141‑1 (MQ=255)

                                                   gTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGt                                                                                    <  2:217336/141‑1 (MQ=255)

                                                     tttCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTAc                                                                                  >  2:111615/1‑141 (MQ=255)

                                                        cAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACatt                                                                               <  1:265114/141‑1 (MQ=255)

                                                         aaGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACatta                                                                              <  1:156585/141‑1 (MQ=255)

                                                              ggTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTa                                                                         <  3:349075/141‑1 (MQ=255)

                                                              ggTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTa                                                                         >  2:309744/1‑141 (MQ=255)

                                                              ggTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTa                                                                         <  3:75516/141‑1 (MQ=255)

                                                              ggTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTa                                                                         >  3:361545/1‑141 (MQ=255)

                                                                  ttGAAACGGCATATAACGCTGTTATGAACACAATTGAAGATACAAGACTTACAGTTGCAAAAGATGCTGCGTACGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAg                                                                     >  4:101160/1‑141 (MQ=255)

                                                                   tGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAga                                                                    >  2:494647/1‑140 (MQ=255)

                                                                      aaCGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCaa                                                                 >  1:152074/1‑141 (MQ=255)

                                                                           cATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAAt                                                            >  1:83262/1‑141 (MQ=255)

                                                                              ataAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTaaa                                                                       >  1:381468/1‑127 (MQ=255)

                                                                              ataAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTaaa                                                                       <  2:381468/127‑1 (MQ=255)

                                                                                  aGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGa                                                     <  1:132803/141‑1 (MQ=255)

                                                                                  aGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGa                                                     <  1:132750/141‑1 (MQ=255)

                                                                                     tGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGaaaa                                                  >  1:516639/1‑141 (MQ=255)

                                                                                     tGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGaaaa                                                  <  2:79512/141‑1 (MQ=255)

                                                                                     tGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGaaaa                                                  <  1:531614/141‑1 (MQ=255)

                                                                                      gTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAGGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAac                                                 >  3:324803/1‑141 (MQ=255)

                                                                                      gTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAac                                                 <  4:274961/141‑1 (MQ=255)

                                                                                       ttATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAaca                                                >  1:153545/1‑141 (MQ=255)

                                                                                         aTGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCACATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAacaca                                              <  3:17132/141‑1 (MQ=255)

                                                                                          tGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGaaaaaaaac                                             <  2:38147/141‑6 (MQ=255)

                                                                                          tGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAacacac                                             >  3:228801/1‑141 (MQ=255)

                                                                                          tGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAacacac                                             <  4:187336/141‑1 (MQ=255)

                                                                                           gAAACCAGTTGACGGTACAATAATCAAAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACAcc                                            <  2:526881/141‑1 (MQ=255)

                                                                                            aaaCCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCa                                           >  1:717822/1‑141 (MQ=255)

                                                                                             aaCCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGGAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCaa                                          <  2:544529/141‑1 (MQ=255)

                                                                                             aaCCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCaa                                          <  1:662041/141‑1 (MQ=255)

                                                                                             aaCCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCaa                                          <  3:329972/141‑1 (MQ=255)

                                                                                              aCCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTTCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCaaa                                         <  4:54863/141‑1 (MQ=255)

                                                                                              aCCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCaaa                                         >  1:303361/1‑141 (MQ=255)

                                                                                              aCCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCaaa                                         <  1:278905/141‑1 (MQ=255)

                                                                                                cAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACt                                       >  1:717134/1‑141 (MQ=255)

                                                                                                  gaagAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACtta                                     >  4:391890/4‑141 (MQ=255)

                                                                                                   ttGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACttat                                    <  1:915853/141‑1 (MQ=255)

                                                                                                      aaGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAg                                 <  4:130740/141‑1 (MQ=255)

                                                                                                      aaGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAAAACTGAAGACTGTATAGAATCAATGGAGTACATTATTGTAAAAGCCAATGAATCACCTGAAAACACACCAAACAAATTAg                                 >  2:284041/1‑141 (MQ=255)

                                                                                                        ggTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCt                               <  1:842735/141‑1 (MQ=255)

                                                                                                         gTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTg                              <  3:249468/141‑1 (MQ=255)

                                                                                                           aCAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTa                            <  2:674874/141‑1 (MQ=255)

                                                                                                            cgaTACTCACAGTTGCAAAAGATGGTGCGTACGCTGCAATAGATAAAGCGAATAATTCTGGAGATTGTATAGAATTAATGGTGTACATTATTGGAATAGCCAATGATTCACGTGAATACACCCCATACTTATTAGCTGTAc                           <  2:755921/139‑1 (MQ=255)

                                                                                                               tACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTa                        >  2:671002/1‑141 (MQ=255)

                                                                                                                aCTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATGCTGAAGATTGTATAGAATTAAAGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTaa                       >  2:744909/1‑141 (MQ=255)

                                                                                                                 cTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTaaa                      <  1:773005/141‑1 (MQ=255)

                                                                                                                 cTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTaaa                      >  1:89029/1‑141 (MQ=255)

                                                                                                                 cTTACAGTTGCAAAAGATGCTGCGTAAGCTGAAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTaaa                      <  4:186401/141‑1 (MQ=255)

                                                                                                                  ttACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAaag                     <  2:606641/141‑1 (MQ=255)

                                                                                                                    aCAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAaagaa                   >  4:180262/1‑141 (MQ=255)

                                                                                                                    aCAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAaagaa                   >  4:49967/1‑141 (MQ=255)

                                                                                                                    aCAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAaagaa                   >  1:659006/1‑141 (MQ=255)

                                                                                                                          gCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGgt             >  1:494136/1‑141 (MQ=255)

                                                                                                                           aaaaaGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGgtg            <  4:304436/140‑1 (MQ=255)

                                                                                                                               aGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAGACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGtgttgt        >  1:761323/1‑141 (MQ=255)

                                                                                                                               aGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGtgttgt        <  1:735188/141‑1 (MQ=255)

                                                                                                                                 aTGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTGttgttg      <  2:33690/141‑1 (MQ=255)

                                                                                                                                 aTGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTGttgttg      >  2:764730/1‑141 (MQ=255)

                                                                                                                                 aTGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACGTAAAGAAGTTGGTGttgttg      >  1:241612/1‑141 (MQ=255)

                                                                                                                                    ctgcGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCGAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGCGTTGTTGATa   >  4:397529/1‑141 (MQ=255)

                                                                                                                                    ctgcGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTCTTAGCTGTACTTAAAGAAGTTGGTGTTGTTGATa   >  1:895747/1‑141 (MQ=255)

                                                                                                                                     tgcGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTGTTGTTGATAg  <  1:143330/141‑1 (MQ=255)

GTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGCAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTGTTGTTGATAG  >  USA300TCH1516_ALE/1238170‑1238443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`