Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 1,607,132 C→T E275K (GAA→AAA)  frc ← Formyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,607,1320CT100.0% 371.5 / NA 100E275K (GAA→AAA) frcFormyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/63);  total (37/63)

TTCTAAATTAAGTTTAATTAAAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTCTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAACCTGGGCACACCATCATTAATGTTAAC  >  USA300TCH1516_ALE/1606996‑1607267
                                                                                                                                        |                                                                                                                                       
ttCTAAATTAAGTTTAATTAAAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTa                                                                                                                                     >  1:458733/1‑141 (MQ=255)
  cTAAATTAAGTTTAATTAAAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATc                                                                                                                                   <  1:372919/141‑1 (MQ=255)
  cTAAATTAAGTTTAATTAAAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTACCATTTTTAATTTTTGTATc                                                                                                                                   >  1:688600/1‑141 (MQ=255)
    aaaTTAAGTTTAATTAAAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCtt                                                                                                                                 >  2:424175/1‑141 (MQ=255)
     aaTTAAGTTTAATTAAAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCttt                                                                                                                                >  1:848060/1‑141 (MQ=255)
         aaGTTTAATTAAAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTAcc                                                                                                                            <  3:343618/141‑1 (MQ=255)
          aGTTTAATTAGAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCg                                                                                                                           <  4:218622/141‑1 (MQ=255)
          aGTTTAATTAAAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCg                                                                                                                           <  1:348666/141‑1 (MQ=255)
                attaaAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTa                                                                                                                     <  2:138368/141‑1 (MQ=255)
                attaaAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTa                                                                                                                     <  2:839302/141‑1 (MQ=255)
                attaaAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTa                                                                                                                     <  2:839380/141‑1 (MQ=255)
                attaaAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTa                                                                                                                     >  1:451618/1‑141 (MQ=255)
                attaaAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTa                                                                                                                     >  1:444425/1‑141 (MQ=255)
                 ttaaAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTAt                                                                                                                    <  2:519563/141‑1 (MQ=255)
                  taaAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTAtt                                                                                                                   <  1:684269/141‑1 (MQ=255)
                   aaaGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTAttt                                                                                                                  <  2:346082/141‑1 (MQ=255)
                   aaaGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTAttt                                                                                                                  >  2:382780/1‑141 (MQ=255)
                     aGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTg                                                                                                                >  1:199288/1‑141 (MQ=255)
                     aGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTg                                                                                                                >  2:29860/1‑141 (MQ=255)
                        tCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTGATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTTATTTTTGTATCTTTTACCGACTTTATTTTGAtt                                                                                                             <  2:87752/141‑1 (MQ=255)
                               ccAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATATTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTa                                                                                                      >  1:336130/1‑141 (MQ=255)
                               ccAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTa                                                                                                      >  2:545496/1‑141 (MQ=255)
                                cAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTAc                                                                                                     <  2:86948/141‑1 (MQ=255)
                                 aGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACa                                                                                                    <  3:248108/141‑1 (MQ=255)
                                   aaCGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGt                                                                                                  <  2:781102/141‑1 (MQ=255)
                                     cGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTGTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTAt                                                                                                <  2:77762/141‑1 (MQ=255)
                                     cGTGCAATCGAACCTCCTGTCCATACAGGTATTGTAAGTCTCGCATCCATATCTAAGGGTAGGTTAGTAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTAt                                                                                                <  1:529931/141‑1 (MQ=255)
                                      gTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATc                                                                                               >  1:120683/1‑141 (MQ=255)
                                        gCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGt                                                                                             >  3:295144/1‑141 (MQ=255)
                                        gCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCAATTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGt                                                                                             >  2:769007/1‑141 (MQ=255)
                                             cGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTAtgtg                                                                                        <  2:323577/141‑1 (MQ=255)
                                              gAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATgtgt                                                                                       <  2:8323/141‑1 (MQ=255)
                                               aaTCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGtt                                                                                      <  4:145629/141‑1 (MQ=255)
                                                  ctcCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTTTTTTTTTATTTTTTGTATGTTTTACCTTCTTTACTTTGATTTTTGGTGTAATTTCGTATGTTTTATc                                                                                   >  2:911413/1‑141 (MQ=255)
                                                  ctcCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTCCAGTATCGTCTGTGTTATc                                                                                   >  2:477168/1‑141 (MQ=255)
                                                  ctcCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATc                                                                                   >  2:324654/1‑141 (MQ=255)
                                                  ctcCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATc                                                                                   <  1:619012/141‑1 (MQ=255)
                                                  ctcCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATc                                                                                   <  1:769007/141‑1 (MQ=255)
                                                    ccTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCGTTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCtt                                                                                 >  4:245650/1‑141 (MQ=255)
                                                     cTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCttt                                                                                >  1:158057/1‑141 (MQ=255)
                                                        ttcAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCt                                                                             <  2:452910/139‑1 (MQ=255)
                                                        tCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCt                                                                             <  2:539324/141‑1 (MQ=255)
                                                            aaCATTTATTGTTTTTCTTCCTTCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTTCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCtttttaaatg                                                                         <  4:361015/141‑7 (MQ=255)
                                                            aaCATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTAttt                                                                         <  3:31715/141‑1 (MQ=255)
                                                            aaCATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTAttt                                                                         >  1:116987/1‑141 (MQ=255)
                                                            aaCATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTAttt                                                                         <  2:370133/141‑1 (MQ=255)
                                                            aaCATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTAttt                                                                         <  2:684641/141‑1 (MQ=255)
                                                              cATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTaa                                                                       <  1:683548/141‑1 (MQ=255)
                                                              cATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTaa                                                                       <  1:708807/141‑1 (MQ=255)
                                                              cATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTaa                                                                       >  3:42570/1‑141 (MQ=255)
                                                               aTTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAAc                                                                      <  3:380798/141‑1 (MQ=255)
                                                               aTTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAAc                                                                      <  1:323394/141‑1 (MQ=255)
                                                                tttATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACt                                                                     <  1:878294/141‑1 (MQ=255)
                                                                 ttATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACtt                                                                    <  3:348106/141‑1 (MQ=255)
                                                                 ttATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACtt                                                                    <  2:526205/141‑1 (MQ=255)
                                                                  tATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTc                                                                   <  2:642394/141‑1 (MQ=255)
                                                                  tATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTc                                                                   <  2:125978/141‑1 (MQ=255)
                                                                    ttGGAATTCGCCCATCCATATCTAATGTTATGCTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGATACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCt                                                                 >  1:715628/1‑141 (MQ=255)
                                                                     tGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCtt                                                                >  1:83314/1‑141 (MQ=255)
                                                                      ggAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCttt                                                               <  1:32820/141‑1 (MQ=255)
                                                                      ggAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCttt                                                               >  1:545126/1‑141 (MQ=255)
                                                                       gAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCTTTTTTTTTTTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTTTCTTTTCTTTTTAATTTCCtttt                                                              >  2:57462/1‑141 (MQ=255)
                                                                        aaTTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTta                                                             <  3:352130/141‑1 (MQ=255)
                                                                         aTTCGCTCATTCATATCTAATGTTATGTTCATAGTCTGTTTTACTTTTTTCCATTTTTAATTTTTTTATCTTTTACCGTCTGTATTTTTATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTCtat                                                            <  2:689265/141‑1 (MQ=255)
                                                                         aTTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTtat                                                            <  1:26940/141‑1 (MQ=255)
                                                                         aTTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTtat                                                            <  2:689249/141‑1 (MQ=255)
                                                                           tCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATCTTTGTATCTTTTACCGACTTTATTTGGAGTATTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATaa                                                          <  1:464263/141‑1 (MQ=255)
                                                                             gCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGTTTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTACCTTCCTTTTATAAcc                                                        <  2:18123/141‑1 (MQ=255)
                                                                             gCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATTTTTTCTATTTAACTTCCTTTTATAAcc                                                        >  2:347501/1‑141 (MQ=255)
                                                                             gCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTAACGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAAcc                                                        >  2:411544/1‑141 (MQ=255)
                                                                               ccatccatATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTa                                                      <  1:809469/141‑1 (MQ=255)
                                                                                  tccatATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTTCTTTTTTAATCTTTGTATCTTTTACCGACTTTATTTTGCTTAGTGGTACATCATCGTATGTGTTTTCTTTTCTTTTTTACTTCCTTTTATATCCtcatt                                                   >  4:218625/1‑137 (MQ=255)
                                                                                  tccatATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACAt                                                   <  1:793435/141‑1 (MQ=255)
                                                                                    catATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATgg                                                 <  2:510891/141‑1 (MQ=255)
                                                                                      tatCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCt                                               >  1:634647/1‑141 (MQ=255)
                                                                                       atCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTg                                              <  2:306917/141‑1 (MQ=255)
                                                                                       atCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTg                                              <  2:494141/141‑1 (MQ=255)
                                                                                         cTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTc                                            <  1:509286/141‑1 (MQ=255)
                                                                                           aaTGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTa                                          <  2:848060/141‑1 (MQ=255)
                                                                                           aaTGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTa                                          <  2:705435/141‑1 (MQ=255)
                                                                                           aaTGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTa                                          <  1:792463/141‑1 (MQ=255)
                                                                                              gttatgttaATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTTTCTATTa                                       >  2:276378/1‑141 (MQ=255)
                                                                                                 atgttaATAGTCTTTTTTTCTTTTTTTTTTTTTTAATTTTTGTATCTTTTACCTACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAATCTACTTTTTTTTCTATTaaat                                    >  2:942950/1‑140 (MQ=255)
                                                                                                  tgttaATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGt                                   >  3:242794/1‑141 (MQ=255)
                                                                                                   gttaATAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTa                                  <  4:89770/141‑1 (MQ=255)
                                                                                                        tAGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTaa                             >  1:788670/1‑141 (MQ=255)
                                                                                                         aGTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAAc                            <  1:273389/141‑1 (MQ=255)
                                                                                                          gTCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAAcc                           <  2:466235/141‑1 (MQ=255)
                                                                                                          gTCTTTTTTACTTTTTGCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTGTCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAAcc                           <  2:902731/141‑1 (MQ=255)
                                                                                                           tCTTTTTTACTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAACCt                          <  4:193045/141‑1 (MQ=255)
                                                                                                              tttttACTTTTTTCCATTTTTAATTTTTCTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAACCTggg                       <  2:702353/141‑1 (MQ=255)
                                                                                                                    cTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAGCCTACATGGCTGTCTATTAAAGTATCTAACCTGGGCACAcc                 <  2:481863/141‑1 (MQ=255)
                                                                                                                    cTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAACCTGGGCACAcc                 <  4:128294/141‑1 (MQ=255)
                                                                                                                    cTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAACCTGGGCACAcc                 <  1:545496/141‑1 (MQ=255)
                                                                                                                    cTTTTTTCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAACCTGGGCACAcc                 <  4:391232/141‑1 (MQ=255)
                                                                                                                      tttttCCATTTTTAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTATCTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAACCTGGGCACACcat               >  2:729585/1‑141 (MQ=255)
                                                                                                                              tttttAATTTTTGTATCTTTTACCGACTTTATTTTGATCAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAACCTGGGCACACCATCATTAATg       >  1:752476/1‑141 (MQ=255)
                                                                                                                               ttttAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAACCTGGGCGCACCATCATTAATGt      <  2:688600/141‑1 (MQ=255)
                                                                                                                                  tAATTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAACCTGGGCACACCATCATTAATGTTaa   >  3:165353/1‑141 (MQ=255)
                                                                                                                                   aaTTTTTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCCTTTATCAAATACATCGCTGTCTATTAATGTATCTAACATGGGCCCACCATCATTAATGTTAAc  >  3:37217/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TTCTAAATTAAGTTTAATTAAAGTTCCTCTTCCAGAACGTGCAATCGAATCTCCTGTCCAAACATTTATTGGAATTCGCCCATCCATATCTAATGTTATGTTAATAGTCTTTTTTACTTTTTTCCATTTTTAATTTCTGTATCTTTTACCGACTTTATTTTGATTAGTGGTACAGTATCGTATGTGTTATCTTTTCTATTTAACTTCCTTTTATAACCTACATGGCTGTCTATTAAAGTATCTAACCTGGGCACACCATCATTAATGTTAAC  >  USA300TCH1516_ALE/1606996‑1607267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑