| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,274,965 | G→C | E106D (GAG→GAC) | mtlR → | Transcriptional regulator MtlR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,274,965 | 0 | G | C | 99.2% | 437.7 / NA | 120 | E106D (GAG→GAC) | mtlR | Transcriptional regulator MtlR |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base C (55/63); minor base A (1/0); total (57/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 120 total aligned reads displayed.
AAAAAAGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAG‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCATTTACT > USA300TCH1516_ALE/2274828‑2275101 | aaGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTa < 2:332063/141‑1 (MQ=255) ggCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAAc < 1:590149/141‑1 (MQ=255) cTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACaa > 4:396896/1‑141 (MQ=255) tACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAGGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAat < 1:715092/141‑1 (MQ=255) cGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAatat < 2:816180/141‑1 (MQ=255) ttGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTACTTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGtt > 1:228315/1‑141 (MQ=255) gCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTa > 1:146917/1‑141 (MQ=255) acaGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCaa < 2:107791/141‑1 (MQ=255) acaGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCaa < 1:281224/141‑1 (MQ=255) acaGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCaa > 1:42071/1‑141 (MQ=255) aTTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGaaa < 2:953897/141‑1 (MQ=255) aTTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGaaa < 1:591573/141‑1 (MQ=255) aTTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCGTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGaaa < 2:955053/141‑1 (MQ=255) tCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATTCGAGCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAAtt > 1:946616/1‑141 (MQ=255) cTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCTAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTg > 3:351933/1‑141 (MQ=255) tGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTgg > 1:587428/1‑141 (MQ=255) tGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTgg < 2:666200/141‑1 (MQ=255) gATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGc < 3:104815/141‑1 (MQ=255) tttAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCAGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAACGAA‑CCAGTTAAACAATATAGTTTAACGCAAGCACTTGGCGt > 4:273468/1‑141 (MQ=255) tAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGttt < 2:193564/141‑1 (MQ=255) tAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGttt < 2:120297/141‑1 (MQ=255) tAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGttt > 1:12299/1‑141 (MQ=255) aaaCGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAACATAGTTTAGCGCAAGAAATTGGCGTTTc > 4:393967/1‑141 (MQ=255) aaCGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCt > 2:648833/1‑141 (MQ=255) cGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGt > 1:835980/1‑141 (MQ=255) gATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTTATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTc < 3:20562/141‑1 (MQ=255) gATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTc < 2:635780/141‑1 (MQ=255) gATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGGTTAGCGCAAGAAATTGGCGTTTCTGTc > 2:938959/1‑141 (MQ=255) ttgtGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGTAAGTAATTATTATATACGCTTTGAGTCAAGCCAAGGAC‑CCAGTTAAACAATGTAGTCTATCGCAAGAAATTGGCGTTTCTGTcc > 4:100844/4‑141 (MQ=255) aTTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTcc < 3:372528/141‑1 (MQ=255) aTTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTcc < 3:298501/141‑1 (MQ=255) tGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGa > 4:369903/1‑141 (MQ=255) gAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATTTAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGAc < 4:155766/141‑1 (MQ=255) gAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGAc < 2:533359/141‑1 (MQ=255) aaGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACt > 4:296483/1‑141 (MQ=255) aaGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACt > 3:170999/1‑141 (MQ=255) aGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTCTTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACtt > 2:210166/1‑141 (MQ=255) gCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACttt < 3:369132/141‑1 (MQ=255) gCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACttt < 2:790257/141‑1 (MQ=255) tCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCa < 2:383429/141‑1 (MQ=255) tGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGAt > 1:806656/1‑141 (MQ=255) acaacaTCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGtt < 2:492868/141‑1 (MQ=255) caacaTCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTa < 4:247558/141‑1 (MQ=255) caTCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAgat < 1:249892/141‑1 (MQ=255) aTCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTCATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAgatg > 1:699153/1‑141 (MQ=255) tCAAACCATTGACTTATCTGTTAAAGAGCATAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAgatga < 3:15029/141‑1 (MQ=255) aaaCCATTGACTTATCTGTTGAAGGGCAGAAAGCAATCAGTATATACGCTTTGATTCAAGCCAAGAAC‑CCAGTTATACTATATAGCTTAGCGCAAGAAAATTGCGTTACTTGCTTGACTTTTACGAAGATGATAgacgttt > 2:434912/1‑136 (MQ=255) aaCCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGAttt < 1:159757/141‑1 (MQ=255) ccATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTag > 4:315135/1‑141 (MQ=255) tGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCt < 2:866269/141‑1 (MQ=255) cTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGa > 2:223340/1‑141 (MQ=255) cTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGa > 4:203363/1‑141 (MQ=255) cTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTaa < 1:925459/141‑1 (MQ=255) tGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTaaa < 1:42082/141‑1 (MQ=255) gTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGACGTTAGATGATTTAGAGCTTGATTTAAAt < 2:292844/141‑1 (MQ=255) aaGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGt > 1:967934/1‑141 (MQ=255) gagCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTAc < 3:375881/141‑1 (MQ=255) gagCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTAc < 2:892809/141‑1 (MQ=255) gCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCa > 1:594561/1‑141 (MQ=255) gCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCa > 3:10077/1‑141 (MQ=255) aGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAAc > 2:514556/1‑141 (MQ=255) aGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAAc < 4:46989/141‑1 (MQ=255) gAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACt < 4:261751/141‑1 (MQ=255) gAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACt < 4:47494/141‑1 (MQ=255) gAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACt < 1:683677/141‑1 (MQ=255) aaaGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTa > 1:120831/1‑141 (MQ=255) aaGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTAt < 2:22851/141‑1 (MQ=255) aGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATc > 1:718860/1‑141 (MQ=255) aGTAATTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATc > 1:636467/1‑141 (MQ=255) aaTTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATCGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCttt < 4:184359/141‑1 (MQ=255) aaTTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCttt < 1:821731/141‑1 (MQ=255) aaTTATTATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCttt > 2:413788/1‑141 (MQ=255) attattATATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTa > 3:287736/1‑141 (MQ=255) ttattaTATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTAt > 1:826958/1‑141 (MQ=255) tattatATACGCTTTGATACAAGCC‑AGGAGCCCAGGTGGGCTTTAGAGGTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTAtc > 2:529994/1‑141 (MQ=255) ttatATACGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTAtctc < 2:587779/141‑1 (MQ=255) tatataCGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCg < 2:796320/141‑1 (MQ=255) atataCGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGa > 3:189969/1‑141 (MQ=255) atataCGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGa > 3:403615/1‑141 (MQ=255) atataCGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGATTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTATCAACTATCTTTATCTCGa > 3:164375/1‑141 (MQ=255) tataCGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGaa > 2:247700/1‑141 (MQ=255) taCGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAg < 1:782885/141‑1 (MQ=255) aCGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATTTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGc > 1:456342/1‑141 (MQ=255) aCGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGc < 1:247955/141‑1 (MQ=255) cGCTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCg < 2:885372/141‑1 (MQ=255) cTTTGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTg > 2:666348/1‑141 (MQ=255) tttGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTgg < 1:511914/141‑1 (MQ=255) ttGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGc < 3:263073/141‑1 (MQ=255) tGATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCg > 3:335546/1‑141 (MQ=255) gATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGa < 2:951688/141‑1 (MQ=255) gATACAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGa > 2:497667/1‑141 (MQ=255) aCAAGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAgg < 1:181230/141‑1 (MQ=255) aaaGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGc < 4:111375/140‑1 (MQ=255) aaGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCa < 2:675320/141‑1 (MQ=255) aGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCAt < 1:960196/141‑1 (MQ=255) aGCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCAt > 3:327566/1‑141 (MQ=255) gCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCAtt < 1:514556/141‑1 (MQ=255) gCCAAGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCAtt < 2:869263/141‑1 (MQ=255) aaGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCATTTAc > 1:210476/1‑141 (MQ=255) aGGAC‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGAACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCATTTACt > 2:924309/1‑141 (MQ=255) | AAAAAAGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAG‑CCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCATTTACT > USA300TCH1516_ALE/2274828‑2275101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |