BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	367,644	G→T	intergenic (‑96/‑62)	nanA ← / → bglK	N‑acetylneuraminate lyase/Beta‑glucoside kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	367,644	0	G	T	100.0%	432.7 / NA	121	intergenic (‑96/‑62)	nanA/bglK	N‑acetylneuraminate lyase/Beta‑glucoside kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (60/61); total (60/61)

Only 100 of 121 total aligned reads displayed.

AACGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTAGTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATGTTTG  >  USA300TCH1516_ALE/367507‑367781

aaCGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCa                                                                                                                                         >  2:391101/1‑141 (MQ=255)

 aCGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTCCACGCTATCATCAAATGTGACAATATCTTGTTGAAAATTGTATTCat                                                                                                                                        >  1:522351/1‑141 (MQ=255)

 aCGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCat                                                                                                                                        >  4:307242/1‑141 (MQ=255)

  cGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCata                                                                                                                                       >  2:536184/1‑141 (MQ=255)

     ttAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATTAAGTTCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATAttt                                                                                                                                    <  2:174393/140‑1 (MQ=255)

     gTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATAttt                                                                                                                                    >  2:542323/1‑141 (MQ=255)

     gTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAAGTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATAttt                                                                                                                                    >  1:676208/1‑141 (MQ=255)

      tAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTa                                                                                                                                   <  1:391492/141‑1 (MQ=255)

        aCGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATg                                                                                                                                 <  1:794373/141‑1 (MQ=255)

         cGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGt                                                                                                                                >  2:57122/1‑141 (MQ=255)

         cGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGt                                                                                                                                <  2:160859/141‑1 (MQ=255)

          gCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGta                                                                                                                               <  1:250092/141‑1 (MQ=255)

          gCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGta                                                                                                                               >  2:758527/1‑141 (MQ=255)

             gCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAtaa                                                                                                                            <  2:878504/141‑1 (MQ=255)

              cATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAtaat                                                                                                                           >  1:859653/1‑141 (MQ=255)

              cATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAtaat                                                                                                                           >  4:30139/1‑141 (MQ=255)

               atatAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAtaatt                                                                                                                          <  1:93541/141‑1 (MQ=255)

                  taAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCGCCTTTAGTGAtaattaat                                                                                                                       <  1:954751/141‑1 (MQ=255)

                  taAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAtaattaat                                                                                                                       <  3:411735/141‑1 (MQ=255)

                   aaaCCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATg                                                                                                                      <  3:6932/141‑1 (MQ=255)

                   aaaCCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAGAATTAATg                                                                                                                      >  1:596005/1‑141 (MQ=255)

                        ttttAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAAt                                                                                                                 <  3:229301/141‑1 (MQ=255)

                         tttAAATCTTTGTTCATTATGAAGTCCCTCCAAAGATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATg                                                                                                                <  1:897942/141‑1 (MQ=255)

                          ttAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGt                                                                                                               >  4:159277/1‑141 (MQ=255)

                          ttAAATCTTTGTTCATCATGACGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGt                                                                                                               >  2:875853/1‑141 (MQ=255)

                           tAAATCTTTTTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATTTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATTAAAATGtt                                                                                                              <  2:901700/141‑1 (MQ=255)

                           tAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGtt                                                                                                              <  1:71169/141‑1 (MQ=255)

                           tAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGtt                                                                                                              <  2:472356/141‑1 (MQ=255)

                             aaTCTTTGTTCATTATGAAGAAAAAAAAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGtttt                                                                                                            <  2:119898/141‑1 (MQ=255)

                                 tttGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCaa                                                                                                        <  3:126027/141‑1 (MQ=255)

                                       cATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCaa                                                                                                  >  2:247817/1‑141 (MQ=255)

                                        aTTATGAAATCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAAt                                                                                                 <  4:150386/141‑1 (MQ=255)

                                          tATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAg                                                                                               >  1:526692/1‑141 (MQ=255)

                                           aTGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGa                                                                                              <  1:343714/141‑1 (MQ=255)

                                            tGAAGTCCCTCCAAAAATCACTAGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATTTAACAATATCTATTTGAAAATTGTATTGATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGaa                                                                                             <  2:798760/141‑1 (MQ=255)

                                             gAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGaaa                                                                                            <  1:651401/141‑1 (MQ=255)

                                              aaGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAAt                                                                                           >  1:937545/1‑141 (MQ=255)

                                               aGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATg                                                                                          >  4:287373/1‑141 (MQ=255)

                                               aGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATg                                                                                          >  2:743035/1‑141 (MQ=255)

                                                gTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATgg                                                                                         <  1:872424/141‑1 (MQ=255)

                                                   ccTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTTATTTTTTTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagt                                                                                      >  4:86361/1‑141 (MQ=255)

                                                    cTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtg                                                                                     >  2:108568/1‑141 (MQ=255)

                                                       cAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtgagt                                                                                  <  3:270310/141‑1 (MQ=255)

                                                        aaaaaTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTa                                                                                 <  4:179770/141‑1 (MQ=255)

                                                         aaaaTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTaa                                                                                <  4:47374/141‑1 (MQ=255)

                                                           aaTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAgg                                                                              <  1:776455/141‑1 (MQ=255)

                                                            aTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGgt                                                                             <  4:245762/141‑1 (MQ=255)

                                                             tCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGgtg                                                                            <  2:443813/141‑1 (MQ=255)

                                                             tCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGgtg                                                                            <  1:246668/141‑1 (MQ=255)

                                                              cATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGgtgt                                                                           <  4:61250/141‑1 (MQ=255)

                                                              cATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGgtgt                                                                           >  3:202951/1‑141 (MQ=255)

                                                              cATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGgtgt                                                                           >  3:413112/1‑141 (MQ=255)

                                                               aTTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTa                                                                          >  1:367453/1‑141 (MQ=255)

                                                                 ttGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTAtt                                                                        >  1:357073/1‑141 (MQ=255)

                                                                         ataGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCa                                                                >  1:471287/1‑141 (MQ=255)

                                                                             tttACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCg                                                            <  1:458420/141‑1 (MQ=255)

                                                                               tACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGat                                                          >  2:657001/1‑141 (MQ=255)

                                                                                 cAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGatat                                                        <  2:258498/141‑1 (MQ=255)

                                                                                 cAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGatat                                                        <  1:436932/141‑1 (MQ=255)

                                                                                       taATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAgg                                                  <  3:287373/141‑1 (MQ=255)

                                                                                         aTTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTGCATCGCAATCGATATTGGAGGC‑a                                               <  1:743035/141‑1 (MQ=255)

                                                                                           tGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACt                                             >  2:450408/1‑141 (MQ=255)

                                                                                            gTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTc                                            <  2:534973/141‑1 (MQ=255)

                                                                                             tAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCa                                           >  3:102615/1‑141 (MQ=255)

                                                                                              aaaCGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTGAATATTTATCTAAATGTAGAGAGTAAGGTGTATTACATCACTGTCGAT‑TTGGGGGCAACTCaa                                          >  2:233373/1‑141 (MQ=255)

                                                                                              aaaCGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCaa                                          <  3:387659/141‑1 (MQ=255)

                                                                                               aaCGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCaaa                                         <  4:264752/141‑1 (MQ=255)

                                                                                                 cGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAATTTCATTAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATAGATATTGGAGGC‑ACTCAAAtt                                       >  2:658877/1‑141 (MQ=255)

                                                                                                 cGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCACAATCGATATTGGAGGC‑ACTCAAAtt                                       >  4:73451/1‑141 (MQ=255)

                                                                                                  gCTATCATAAAATGTAACAATATCTTTTTTTATATTGTTTTCATATTTATGTATAATTAATGAAACTGTTTTTCAAAATAAATAGAAATGGAGTGAGTAAGGTGTATTACATCACAATCGATATTGGAGGC‑ACACAAATTa                                      >  2:472181/1‑141 (MQ=255)

                                                                                                  gCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTa                                      <  2:333565/141‑1 (MQ=255)

                                                                                                   cTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTaa                                     >  1:98722/1‑141 (MQ=255)

                                                                                                    tATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTaaa                                    <  4:124909/141‑1 (MQ=255)

                                                                                                    tATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTaaa                                    <  1:424253/141‑1 (MQ=255)

                                                                                                     aTCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAAt                                   >  1:968167/1‑141 (MQ=255)

                                                                                                      tCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATCTTTATTTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATc                                  >  1:221384/1‑141 (MQ=255)

                                                                                                       cATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCg                                 <  1:651789/141‑1 (MQ=255)

                                                                                                       cATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCg                                 <  1:44605/141‑1 (MQ=255)

                                                                                                       cATAAAATGTAACAATATCTTTGTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCg                                 <  2:525122/141‑1 (MQ=255)

                                                                                                       cATAAAAGTTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCg                                 <  2:911988/141‑1 (MQ=255)

                                                                                                        aTAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCgg                                >  2:908496/1‑141 (MQ=255)

                                                                                                         tAAAATGTAACAATATCTTTTTTTTTTTTTTTTTTATATTTATGTATAATTAATGAAAATTTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCACAATCAATATTGGAGGC‑ACTCAAATTTAATcttt                               >  2:641651/1‑138 (MQ=255)

                                                                                                         tAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGc                               <  2:367453/141‑1 (MQ=255)

                                                                                                             aTGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGtt                           >  4:136438/1‑141 (MQ=255)

                                                                                                                tAACAATATCTTTTTGAAACTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAAGAGAAATGGGGTGAGAAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTAtt                        >  3:328953/1‑141 (MQ=255)

                                                                                                                  aCAATATCTTTTTTTTTTTTTTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTAAATCGAAATAGATCTTTTTGGT‑TCTCTAATTAAATCGGCAGTTATTGa                      >  2:732701/1‑141 (MQ=255)

                                                                                                                  aCAATATCTTTTTGAAAATTGTTTTCATATTTAAGTAGAATCAATGAAAATGTTTTTGAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGTC‑ACTTAAATTAAATAGACAGTTATTgc                      >  2:54787/1‑140 (MQ=255)

                                                                                                                  aCAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGt                            >  3:122668/1‑135 (MQ=255)

                                                                                                                  aCAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGa                      >  1:755557/1‑141 (MQ=255)

                                                                                                                  aCAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGa                      >  1:405274/1‑141 (MQ=255)

                                                                                                                     atatCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGATaa                   >  1:150907/1‑141 (MQ=255)

                                                                                                                       atCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGATAAGc                 >  3:344094/1‑141 (MQ=255)

                                                                                                                        tCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGATAAGCa                <  2:754706/141‑1 (MQ=255)

                                                                                                                            tttGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGATAAGCAATTg            <  3:99949/141‑1 (MQ=255)

                                                                                                                            tttGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGATAAGCAATTg            <  1:406990/141‑1 (MQ=255)

                                                                                                                            tttGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGATAAGCAATTg            <  1:869814/141‑1 (MQ=255)

                                                                                                                                    ttGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCTAAATTTATAAAAATGCAGTCAGTAAGCAGTATTACATCGCAATCGATATTGGAGGA‑ACTGAGATTATATCGGCATTTATTGATAAGCAATTGattttatt    >  2:588880/1‑134 (MQ=255)

                                                                                                                                    ttGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATGtt    >  2:113728/1‑141 (MQ=255)

                                                                                                                                     tGTATTCATATTTATGTATAATTAATGAAAATGTTTTACAAAATCAATAGAAATGAAGTGAGTAAGGTCTAGTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCACATATTGATAAGCAAGTGAATATGttt   >  2:656611/1‑141 (MQ=255)

                                                                                                                                      gTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATGTTTg  <  2:350941/141‑1 (MQ=255)

AACGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTAGTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGC‑ACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATGTTTG  >  USA300TCH1516_ALE/367507‑367781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`