BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,315,088	T→A	K61I (AAA→ATA)	ndhE ←	NAD(P)H‑quinone oxidoreductase subunit 4L, chloroplastic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,315,088	0	T	A	100.0%	379.3 / NA	103	K61I (AAA→ATA)	ndhE	NAD(P)H‑quinone oxidoreductase subunit 4L, chloroplastic
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (56/47); total (56/47)

Only 100 of 109 total aligned reads displayed.

AATCCCTCTTAATGTCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATTTATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAATTA  >  USA300TCH1516_ALE/2314952‑2315225

aaTCCCTCTTAATGTCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATc                                                                                                                                       >  2:807025/1‑141 (MQ=255)

aaTCCCTCTTAATGTCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATc                                                                                                                                       >  2:807268/1‑141 (MQ=255)

aaTCCCTCTTAATGTCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATc                                                                                                                                       <  1:405117/141‑1 (MQ=255)

  tCCCTCTTAATGTCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCgg                                                                                                                                     <  4:114765/141‑1 (MQ=255)

   cccTCTTAATGTCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGc                                                                                                                                    >  2:576385/1‑141 (MQ=255)

     ctctTAATGTCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCaa                                                                                                                                  >  2:441252/1‑141 (MQ=255)

          aaTGTCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGcc                                                                                                                             >  1:232631/1‑141 (MQ=255)

          aaTGTCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGcc                                                                                                                             >  4:370584/1‑141 (MQ=255)

           aTGTCGCATATATAATAATGACGCTAATGGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGccc                                                                                                                            <  3:18155/141‑1 (MQ=255)

            tGTCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGcccc                                                                                                                           <  3:56668/141‑1 (MQ=255)

              tCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTg                                                                                                                         >  1:724425/1‑141 (MQ=255)

              tCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTg                                                                                                                         <  1:354238/141‑1 (MQ=255)

                 cATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGccc                                                                                                                      <  1:618029/141‑1 (MQ=255)

                  atatatAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCca                                                                                                                     <  1:468187/141‑1 (MQ=255)

                     tataATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCcacac                                                                                                                  <  1:588198/141‑1 (MQ=255)

                     tataATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCcacac                                                                                                                  <  2:649121/141‑1 (MQ=255)

                          taatGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTa                                                                                                             >  3:181119/1‑141 (MQ=255)

                          taatGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTa                                                                                                             >  2:635728/1‑141 (MQ=255)

                             tGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGta                                                                                                          <  4:415372/141‑1 (MQ=255)

                               aCGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCTCACACACTTAGtaat                                                                                                        >  1:800438/1‑141 (MQ=255)

                               aCGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGtaat                                                                                                        >  2:196489/1‑141 (MQ=255)

                                cGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGtaata                                                                                                       >  4:301442/1‑141 (MQ=255)

                                 gCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGtaataa                                                                                                      <  1:238157/141‑1 (MQ=255)

                                      ttGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCGTATATATCNGCAATTGCCCCTGCCCACACACTTAGTAATAATATTg                                                                                                 <  3:338569/141‑1 (MQ=255)

                                        ggAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGt                                                                                               >  1:85019/1‑141 (MQ=255)

                                        ggAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGt                                                                                               >  1:903537/1‑141 (MQ=255)

                                          aaTTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTAc                                                                                             >  2:948794/1‑141 (MQ=255)

                                          aaTTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATAATATATATTGGCAATTGCTCCTGCCCACACACTTAGTAATAATATTGGTAc                                                                                             >  2:304197/1‑141 (MQ=255)

                                            ttGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACaa                                                                                           >  2:11448/1‑141 (MQ=255)

                                               cAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTc                                                                                        >  2:785257/1‑141 (MQ=255)

                                                aGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCt                                                                                       <  1:526127/141‑1 (MQ=255)

                                                  tGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGc                                                                                     <  1:8530/141‑1 (MQ=255)

                                                  tGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGc                                                                                     <  2:112682/141‑1 (MQ=255)

                                                  tGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGc                                                                                     <  1:400827/141‑1 (MQ=255)

                                                  tGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGc                                                                                     <  3:371073/141‑1 (MQ=255)

                                                  tGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGc                                                                                     <  2:4176/141‑1 (MQ=255)

                                                  tGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGc                                                                                     <  3:162187/141‑1 (MQ=255)

                                                       cTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCaaaaa                                                                                <  1:944652/141‑1 (MQ=255)

                                                        tataCGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAAt                                                                               >  2:664076/1‑141 (MQ=255)

                                                         ataCGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAAtt                                                                              >  3:121253/1‑141 (MQ=255)

                                                            cGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGAc                                                                           <  1:92038/141‑1 (MQ=255)

                                                            cGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGAc                                                                           <  2:702566/141‑1 (MQ=255)

                                                                 gCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATcc                                                                      >  2:122573/1‑141 (MQ=255)

                                                                  cacacaTAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATccc                                                                     <  4:406348/141‑1 (MQ=255)

                                                                   acacaTAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGGAATGGCTCTTTATCTTATATGTGGGCTTTTTCTCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATcccc                                                                    >  3:80693/1‑141 (MQ=255)

                                                                     acaTAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAg                                                                  >  2:772502/1‑141 (MQ=255)

                                                                      caTAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGa                                                                 >  3:167644/1‑141 (MQ=255)

                                                                      caTAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGa                                                                 >  4:277298/1‑141 (MQ=255)

                                                                       ttAAAATTTCAGGGACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGAt                                                                <  2:35367/140‑1 (MQ=255)

                                                                       aTAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGAt                                                                <  1:279105/141‑1 (MQ=255)

                                                                         aaaaTTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATaa                                                              >  1:410818/1‑141 (MQ=255)

                                                                         aaaaTTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATaa                                                              >  1:410817/1‑141 (MQ=255)

                                                                          aaaTTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCACTTGCCCCTGCCCACACACTTCCTAATAATATTGTTACAAGTTTGCAAAAATTGACTATCCCCAGATaat                                                             <  4:10540/141‑2 (MQ=255)

                                                                           aaTTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAAc                                                            <  1:122573/141‑1 (MQ=255)

                                                                             ttGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACtt                                                          >  2:95568/1‑140 (MQ=255)

                                                                             ttGCAGTTACTAAAAATGATGATGAAATCGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTg                                                          <  3:367262/141‑1 (MQ=255)

                                                                              tGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATTTTCCCAAAGGCGCCGTGTTACATACTTTGGAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGc                                                         >  3:80441/1‑141 (MQ=255)

                                                                              tGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGc                                                         >  2:229042/1‑141 (MQ=255)

                                                                               gCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCg                                                        >  2:888754/1‑141 (MQ=255)

                                                                                cAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGt                                                       >  2:931481/1‑141 (MQ=255)

                                                                                 aGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGtt                                                      >  2:222509/1‑141 (MQ=255)

                                                                                 aGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGtt                                                      >  1:49192/1‑141 (MQ=255)

                                                                                 aGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATAGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGtt                                                      >  1:759742/1‑141 (MQ=255)

                                                                                 aGTTACTAAAAATGAAGATGAAATTGTAACTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGACCCTGCCCACACACTTACTAATAATATCGGTACAAGACTGCAAAAATCAACAATCCCCAGATAAACTGCGtt                                                      >  2:248360/1‑141 (MQ=255)

                                                                                  gTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTa                                                     >  2:359648/1‑141 (MQ=255)

                                                                                  gTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTa                                                     <  2:315668/141‑1 (MQ=255)

                                                                                    tACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATg                                                   <  2:816213/141‑1 (MQ=255)

                                                                                     aCTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGa                                                  <  1:159624/141‑1 (MQ=255)

                                                                                      cTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGAt                                                 >  1:165668/1‑141 (MQ=255)

                                                                                      cTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGAt                                                 <  2:730312/141‑1 (MQ=255)

                                                                                      atAAAAATTGTGATGAAATTGTAATAAAAAGCAAACGACCTTTATCATATACATCGGCAACAGACACAGCCAACACATCTTGTACTATTCTTGAAAAAAAAAAAAAAAAATTGACAATCCCCAGATAAACTGCGTTATGAt                                                 <  2:868650/140‑1 (MQ=255)

                                                                                          aaaTGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGt                                             >  2:948495/1‑141 (MQ=255)

                                                                                          aaaTGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGt                                             >  3:209534/1‑141 (MQ=255)

                                                                                            atgatgatgAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTgg                                           >  2:697617/1‑141 (MQ=255)

                                                                                            atgataatgAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTgg                                           >  2:726698/1‑141 (MQ=255)

                                                                                             tgatgatgaAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGt                                          <  3:254937/141‑1 (MQ=255)

                                                                                              gatgatgaAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTAAAAAAAAAAAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTa                                         <  4:267041/141‑1 (MQ=255)

                                                                                                tgatgaAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAGTCCCCAGATAAACTGCGTTATGATAAGTGGTAAg                                       >  1:897355/1‑141 (MQ=255)

                                                                                                 gatgaAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGt                                      <  3:272118/141‑1 (MQ=255)

                                                                                                  atgaAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAGATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTa                                     >  1:463165/1‑141 (MQ=255)

                                                                                                   tgaAATTGTAATTCTCAGCAATCGCCCTTTATCATATATAGCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCACCAGATAAACTGCGTTATGATAAGTGGTAAGTAc                                    >  3:171451/1‑141 (MQ=255)

                                                                                                        ttGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAAcc                               <  4:392348/141‑1 (MQ=255)

                                                                                                         ataagctctcAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCa                              <  2:675691/135‑1 (MQ=255)

                                                                                                            aaTTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAAtt                           >  4:124195/1‑141 (MQ=255)

                                                                                                            aaTTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAAtt                           >  4:74476/1‑141 (MQ=255)

                                                                                                            aaTTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACGTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAAtt                           <  1:73671/141‑1 (MQ=255)

                                                                                                             aTTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAAttt                          >  4:220192/1‑141 (MQ=255)

                                                                                                                 tcAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGcc                      <  4:127623/141‑1 (MQ=255)

                                                                                                                 tcAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGcc                      >  3:142988/1‑141 (MQ=255)

                                                                                                                  cAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGccc                     <  3:365242/141‑1 (MQ=255)

                                                                                                                       aTCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTAttt                >  2:773057/1‑141 (MQ=255)

                                                                                                                        tCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTATTAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTg               >  1:399476/1‑141 (MQ=255)

                                                                                                                          gCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGtc             >  4:361701/1‑141 (MQ=255)

                                                                                                                              tttATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGtccaat         <  1:933088/141‑1 (MQ=255)

                                                                                                                                    atatatatCGGCAATTGCCCCTGCCCACACACTTAGTAataata                                                                                                    >  4:180302/1‑44 (MQ=255)

                                                                                                                                    atatatatCGGCAATTGCCCCTGCCCACACACTTAGTAataata                                                                                                    <  3:180302/44‑1 (MQ=255)

                                                                                                                                    atatatatCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAAtt   >  2:326140/1‑141 (MQ=255)

                                                                                                                                    atatatatCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAAtt   <  3:56213/141‑1 (MQ=255)

                                                                                                                                    atatatatCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAAtt   <  1:716298/141‑1 (MQ=255)

                                                                                                                                    atatatatCGGCAATTGCCCCTGCCCACACACTTAGTAAT‑ATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAATTa  >  2:927845/1‑141 (MQ=255)

AATCCCTCTTAATGTCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATTTATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAATTA  >  USA300TCH1516_ALE/2314952‑2315225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`