BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,342,380	A→C	L76V (TTG→GTG)	USA300HOU_RS11950 ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,342,380	0	A	C	99.2%	435.9 / NA	118	L76V (TTG→GTG)	USA300HOU_RS11950	putative hydrolase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (50/67); total (51/67)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 118 total aligned reads displayed.

GACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCAAATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCC  >  USA300TCH1516_ALE/2342243‑2342516

gACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATc                                                                                                                                       <  1:264571/141‑1 (MQ=255)

gACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTGTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATc                                                                                                                                       >  3:278053/1‑141 (MQ=255)

  cATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAg                                                                                                                                     <  1:853769/141‑1 (MQ=255)

   aTTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGa                                                                                                                                    <  1:589808/141‑1 (MQ=255)

   aTTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGa                                                                                                                                    <  1:757821/141‑1 (MQ=255)

    ttATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGaa                                                                                                                                   >  4:271600/1‑141 (MQ=255)

     tataTTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGaaa                                                                                                                                  >  2:420027/1‑141 (MQ=255)

     tataTTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGaaa                                                                                                                                  <  4:84510/141‑1 (MQ=255)

      atatTGCTCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGaaaa                                                                                                                                 >  1:767334/1‑141 (MQ=255)

      atatTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGaaaa                                                                                                                                 >  3:167430/1‑141 (MQ=255)

          tGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGata                                                                                                                             <  2:893495/141‑1 (MQ=255)

          tGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGata                                                                                                                             <  1:431828/141‑1 (MQ=255)

            cgcAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATg                                                                                                                           >  4:48338/1‑141 (MQ=255)

             gcAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGt                                                                                                                          >  4:185503/1‑141 (MQ=255)

                gAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAAt                                                                                                                       <  2:251600/141‑1 (MQ=255)

                 aaaTATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATg                                                                                                                      <  3:262454/141‑1 (MQ=255)

                 aaaTATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATg                                                                                                                      <  2:510683/141‑1 (MQ=255)

                     atGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGttt                                                                                                                  >  4:88367/1‑141 (MQ=255)

                       gACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTaa                                                                                                                >  2:594729/1‑141 (MQ=255)

                        aCGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGGAAAGATATGTAATGGTTTaaa                                                                                                               >  3:331874/1‑141 (MQ=255)

                        aCGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATAGGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTaaa                                                                                                               <  4:136538/141‑1 (MQ=255)

                          gTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTATAACTCACATCAGAAAAGATATGTAAATGTTTAAACg                                                                                                             >  4:187052/1‑141 (MQ=255)

                          gTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACg                                                                                                             <  1:135714/141‑1 (MQ=255)

                          gTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACg                                                                                                             <  1:620861/141‑1 (MQ=255)

                           tAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGt                                                                                                            <  1:594729/141‑1 (MQ=255)

                             atatatCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCa                                                                                                          <  2:153429/141‑1 (MQ=255)

                             atatatCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCa                                                                                                          <  4:86326/141‑1 (MQ=255)

                              tatatCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCat                                                                                                         <  2:942415/141‑1 (MQ=255)

                               atatcttcaatatcttcaAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCata                                                                                                        <  1:162221/141‑1 (MQ=255)

                                     tcaatatcttcaaATAAAAATGGTTCGCTCAATACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACt                                                                                                  <  4:380921/141‑1 (MQ=255)

                                     tcaatatcttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACt                                                                                                  <  2:173854/141‑1 (MQ=255)

                                           tcttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATcc                                                                                            >  2:228212/1‑141 (MQ=255)

                                           tcttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATcc                                                                                            >  2:651955/1‑141 (MQ=255)

                                             ttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCAt                                                                                          <  1:838219/141‑1 (MQ=255)

                                             ttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCAt                                                                                          <  1:838269/141‑1 (MQ=255)

                                             ttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCAt                                                                                          >  2:208960/1‑141 (MQ=255)

                                             ttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCACAAAAGCTATGACATGGTTTAAACGTCATATTAACTTGATCCAt                                                                                          <  4:121556/141‑1 (MQ=255)

                                              tcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCAtt                                                                                         >  2:535804/1‑141 (MQ=255)

                                                    aaaaaTGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATcc                                                                                   >  4:192531/1‑141 (MQ=255)

                                                    aaaaaTGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATcc                                                                                   <  1:387370/141‑1 (MQ=255)

                                                     aaaaTGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTTTTACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCa                                                                                  >  1:190924/1‑141 (MQ=255)

                                                     aaaaTGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCa                                                                                  >  2:738868/1‑141 (MQ=255)

                                                      aaaTGGTTCGCTCTAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCGAGTGAACATTTTAGCACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCAt                                                                                 <  2:753902/141‑1 (MQ=255)

                                                       aaTGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATg                                                                                >  2:128963/1‑141 (MQ=255)

                                                        aTGGTTCTCTCAAGACTTTCTTTTTATATGTTTATGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGACCTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGa                                                                               <  4:313103/141‑1 (MQ=255)

                                                        aTGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGa                                                                               <  1:68846/141‑1 (MQ=255)

                                                         tGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGac                                                                              <  1:364661/141‑1 (MQ=255)

                                                          ggTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGaca                                                                             <  1:259231/141‑1 (MQ=255)

                                                           gTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGacac                                                                            >  1:374139/1‑141 (MQ=255)

                                                             tCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACtt                                                                          <  1:518130/141‑1 (MQ=255)

                                                             tCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACtt                                                                          <  2:558197/141‑1 (MQ=255)

                                                              cGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACttt                                                                         >  2:457543/1‑141 (MQ=255)

                                                                cTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGa                                                                       <  1:383307/141‑1 (MQ=255)

                                                                 tCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGaa                                                                      <  2:299279/141‑1 (MQ=255)

                                                                 tCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGaa                                                                      <  1:713082/141‑1 (MQ=255)

                                                                     gACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGt                                                                  >  1:158328/1‑141 (MQ=255)

                                                                     gACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGt                                                                  <  1:216040/141‑1 (MQ=255)

                                                                     gACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGt                                                                  <  2:296630/141‑1 (MQ=255)

                                                                     gACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGt                                                                  <  1:466906/141‑1 (MQ=255)

                                                                      aCTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTa                                                                 <  1:312761/141‑1 (MQ=255)

                                                                      aCTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTa                                                                 <  1:855447/141‑1 (MQ=255)

                                                                         ttctttTTAAATGTTTCTAATTCAACTTTTGCTCGATTAGGCTAATGGAACGTTTTATAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAGATGTACta                                                              <  1:920139/141‑2 (MQ=255)

                                                                          tctttTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTc                                                             <  4:344928/141‑1 (MQ=255)

                                                                           ctttTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTcc                                                            >  2:668021/1‑141 (MQ=255)

                                                                            tttttATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCt                                                           >  3:14014/1‑141 (MQ=255)

                                                                             ttttATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTg                                                          <  2:861855/141‑1 (MQ=255)

                                                                              tttATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGt                                                         <  4:62601/141‑1 (MQ=255)

                                                                                    tGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATAc                                                   >  2:212115/1‑141 (MQ=255)

                                                                                        tcggATTCAACAGATTCGCCATTAGTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACGTGATCCATTGTATCCATGACACTTTGAAATGTACTTCCTGTCAAGAcagca                                               <  2:963097/138‑1 (MQ=255)

                                                                                        tCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATAcagca                                               >  2:456455/1‑141 (MQ=255)

                                                                                          tGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGt                                             >  1:819673/1‑141 (MQ=255)

                                                                                          tGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGt                                             >  2:86394/1‑141 (MQ=255)

                                                                                           taTTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTg                                            <  3:156502/140‑1 (MQ=255)

                                                                                           gATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTg                                            <  1:518690/141‑1 (MQ=255)

                                                                                             ttCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGAt                                          <  2:689212/141‑1 (MQ=255)

                                                                                               cAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCa                                        >  2:629707/1‑141 (MQ=255)

                                                                                               cAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCa                                        >  1:882131/1‑141 (MQ=255)

                                                                                                   atatTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTc                                    >  2:245104/1‑141 (MQ=255)

                                                                                                   atatTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTc                                    >  2:874669/1‑141 (MQ=255)

                                                                                                   atatTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTc                                    >  1:672720/1‑141 (MQ=255)

                                                                                                    tatTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCa                                   <  3:218086/141‑1 (MQ=255)

                                                                                                      ttCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCatt                                 <  2:729792/141‑1 (MQ=255)

                                                                                                       tCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCatta                                <  1:266566/141‑1 (MQ=255)

                                                                                                        cGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCattat                               <  2:146954/141‑1 (MQ=255)

                                                                                                         gCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCattatt                              >  1:218729/1‑141 (MQ=255)

                                                                                                             ttattCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATACATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCAttattatta                          <  1:255582/141‑1 (MQ=255)

                                                                                                                  ccAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTAttagttag                     >  2:797766/1‑141 (MQ=255)

                                                                                                                  ccAAGTGAACATTTTAGAACTCAAATGAAAAAATATAGTTAAGGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTAttagttag                     >  2:43660/1‑141 (MQ=255)

                                                                                                                   cAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATtagttagt                    >  1:305120/1‑141 (MQ=255)

                                                                                                                           cATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCcaa            <  4:222020/141‑1 (MQ=255)

                                                                                                                            aTTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCcaac           >  2:591297/1‑141 (MQ=255)

                                                                                                                            aTTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCcaac           >  2:792378/1‑141 (MQ=255)

                                                                                                                             ttttAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCcaaca          >  1:899182/1‑141 (MQ=255)

                                                                                                                                tAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAAtt       <  4:251615/141‑1 (MQ=255)

                                                                                                                                tAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAAtt       <  1:796901/141‑1 (MQ=255)

                                                                                                                                  gAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAAtttt     >  2:29628/1‑141 (MQ=255)

                                                                                                                                  gAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAAtttt     <  1:165649/141‑1 (MQ=255)

                                                                                                                                    aCTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTc   <  1:873458/141‑1 (MQ=255)

                                                                                                                                    aCTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTc   <  2:581246/141‑1 (MQ=255)

                                                                                                                                     cTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTcc  <  2:305120/141‑1 (MQ=255)

GACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCAAATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCC  >  USA300TCH1516_ALE/2342243‑2342516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`