BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,388,866	A→G	intergenic (‑149/+542)	sarV ← / ← moaA	HTH‑type transcriptional regulator SarV/GTP 3',8‑cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,388,866	0	A	G	100.0%	370.9 / NA	103	intergenic (‑149/+542)	sarV/moaA	HTH‑type transcriptional regulator SarV/GTP 3',8‑cyclase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (51/52); total (51/52)

Only 100 of 103 total aligned reads displayed.

TCCTATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGACAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGAAAT  >  USA300TCH1516_ALE/2388729‑2389000

tcctATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCaa                                                                                                                                     <  3:250557/141‑1 (MQ=255)

   tATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACtt                                                                                                                                  <  2:606218/141‑1 (MQ=255)

   tATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACtt                                                                                                                                  <  2:606231/141‑1 (MQ=255)

     tGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACttgt                                                                                                                                <  1:860648/141‑1 (MQ=255)

     tGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACttgt                                                                                                                                <  2:843406/141‑1 (MQ=255)

     tGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACttgt                                                                                                                                <  2:70371/141‑1 (MQ=255)

         tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTgttgtt                                                                                                                            >  3:55533/1‑141 (MQ=255)

         tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTgttgtt                                                                                                                            >  2:270800/1‑141 (MQ=255)

          cttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGttt                                                                                                                           >  2:774590/1‑141 (MQ=255)

          cttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGttt                                                                                                                           <  3:296862/141‑1 (MQ=255)

               tAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCg                                                                                                                      >  2:679217/1‑141 (MQ=255)

               tAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCg                                                                                                                      >  4:20082/1‑141 (MQ=255)

                aGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGa                                                                                                                     >  1:723358/1‑141 (MQ=255)

                  aTGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTTGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGaca                                                                                                                   >  3:262920/1‑141 (MQ=255)

                  aTGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGaca                                                                                                                   <  1:87358/141‑1 (MQ=255)

                   tGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGacaa                                                                                                                  <  4:276764/141‑1 (MQ=255)

                    gTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGacaac                                                                                                                 >  1:657618/1‑141 (MQ=255)

                    gTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGacaac                                                                                                                 >  1:92880/1‑141 (MQ=255)

                    gTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGacaac                                                                                                                 >  4:173487/1‑141 (MQ=255)

                     tttGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGacaaca                                                                                                                >  2:901019/1‑141 (MQ=255)

                       tGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACAtt                                                                                                              >  1:410258/1‑141 (MQ=255)

                       tGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACAtt                                                                                                              >  1:922180/1‑141 (MQ=255)

                        gTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACAttt                                                                                                             >  2:38977/1‑141 (MQ=255)

                           aTTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTtata                                                                                                          <  1:148699/141‑1 (MQ=255)

                           aTTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTtata                                                                                                          <  3:360779/141‑1 (MQ=255)

                            ttCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATAc                                                                                                         <  3:249062/141‑1 (MQ=255)

                            ttCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATAc                                                                                                         <  1:608068/141‑1 (MQ=255)

                              cATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCt                                                                                                       <  1:309842/141‑1 (MQ=255)

                              cATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCt                                                                                                       >  4:113909/1‑141 (MQ=255)

                                tattattatACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTaa                                                                                                     <  1:165093/141‑1 (MQ=255)

                                 attattatACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTaaa                                                                                                    <  3:312804/141‑1 (MQ=255)

                                 attattatACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTaaa                                                                                                    <  2:283456/141‑1 (MQ=255)

                                    attatACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTaaaaaa                                                                                                 >  1:952436/1‑141 (MQ=255)

                                               aCTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACa                                                                                      >  1:274293/1‑141 (MQ=255)

                                                 ttAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAAc                                                                                    <  1:220061/141‑1 (MQ=255)

                                                   aaaTTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTc                                                                                  <  1:492324/141‑1 (MQ=255)

                                                   aaaTTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTc                                                                                  <  1:42647/141‑1 (MQ=255)

                                                    aaTTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCa                                                                                 >  2:812156/1‑141 (MQ=255)

                                                     aTTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCaa                                                                                >  1:868250/1‑141 (MQ=255)

                                                     aTTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCaa                                                                                >  3:414269/1‑141 (MQ=255)

                                                      tttATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTAGACAACATTTTTGCTTAAAAAAGATATATTACAACTCAAt                                                                               >  1:187328/1‑141 (MQ=255)

                                                         attatATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGc                                                                            <  4:174503/141‑1 (MQ=255)

                                                          ttatATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCa                                                                           >  1:115139/1‑141 (MQ=255)

                                                          ttatATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCa                                                                           >  4:327561/1‑141 (MQ=255)

                                                            atatatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTATAACTCAATGGCATg                                                                         >  2:410786/1‑141 (MQ=255)

                                                            atatatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATg                                                                         >  3:275446/1‑141 (MQ=255)

                                                            atatatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATg                                                                         <  1:29836/141‑1 (MQ=255)

                                                              atatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGa                                                                       <  3:412484/141‑1 (MQ=255)

                                                              atatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGa                                                                       <  1:461311/141‑1 (MQ=255)

                                                              atatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGa                                                                       <  3:99374/141‑1 (MQ=255)

                                                              atatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGa                                                                       <  2:689578/141‑1 (MQ=255)

                                                               tatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAg                                                                      >  4:361338/1‑141 (MQ=255)

                                                               tatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAg                                                                      >  1:126105/1‑141 (MQ=255)

                                                               tatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAg                                                                      >  2:161595/1‑141 (MQ=255)

                                                                 tCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAgtg                                                                    <  2:542255/141‑1 (MQ=255)

                                                                  cAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAgtgt                                                                   <  1:40455/141‑1 (MQ=255)

                                                                  cAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAgtgt                                                                   <  2:823680/141‑1 (MQ=255)

                                                                        tAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATc                                                             >  2:398110/1‑141 (MQ=255)

                                                                         aGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCa                                                            >  2:873074/1‑141 (MQ=255)

                                                                          gCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCaa                                                           >  1:251016/1‑141 (MQ=255)

                                                                          gCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCaa                                                           >  1:379122/1‑141 (MQ=255)

                                                                           cATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAAt                                                          >  4:352472/1‑141 (MQ=255)

                                                                            aTTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCCTGGAGTGTGACATCAATg                                                         <  2:206769/141‑1 (MQ=255)

                                                                             ttttATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGt                                                        >  4:173179/1‑141 (MQ=255)

                                                                              tttATCTATTATAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGtt                                                       <  2:428755/141‑1 (MQ=255)

                                                                                tATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTaa                                                     >  2:248801/1‑141 (MQ=255)

                                                                                    tATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGaa                                                 <  1:393014/141‑1 (MQ=255)

                                                                                      ttACAAATATTAAATTGATGGCTATATTTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATAAATAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAAcc                                               <  2:21892/141‑1 (MQ=255)

                                                                                          aaaTATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTa                                           >  1:950649/1‑141 (MQ=255)

                                                                                          aaaTATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTa                                           <  1:388861/141‑1 (MQ=255)

                                                                                          aaaTATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTa                                           >  1:950797/1‑141 (MQ=255)

                                                                                          aaaTATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTa                                           <  3:195154/141‑1 (MQ=255)

                                                                                           aaTATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTaa                                          <  3:20082/141‑1 (MQ=255)

                                                                                           aaTATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTaa                                          <  1:38244/141‑1 (MQ=255)

                                                                                           aaTATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGAGCAACTTGTTGTTTATTCGACAACAAAAAAAAAAAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTaa                                          <  2:859179/141‑1 (MQ=255)

                                                                                               ttAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGAt                                      >  3:45238/1‑141 (MQ=255)

                                                                                                tAAATTGCTAGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCGTGGAGTGTGACATCAATGTTAAAGAACCACTAAAGAtt                                     <  1:512092/141‑1 (MQ=255)

                                                                                                  aaTTGCGGGCTATATATTGTTTAAATTAATATAGTTTAGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATtta                                   >  2:127072/1‑141 (MQ=255)

                                                                                                    ttGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATttatt                                 <  2:92880/141‑1 (MQ=255)

                                                                                                        tGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAAGATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGt                             >  4:405823/1‑141 (MQ=255)

                                                                                                          gCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAg                           <  4:272222/141‑1 (MQ=255)

                                                                                                            tataTCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTg                         >  1:553644/1‑141 (MQ=255)

                                                                                                                tCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTc                     <  3:173487/141‑1 (MQ=255)

                                                                                                                tCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTc                     >  1:498757/1‑141 (MQ=255)

                                                                                                                  ttGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCtt                   <  2:2243/141‑1 (MQ=255)

                                                                                                                  ttGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCtt                   >  1:678321/1‑141 (MQ=255)

                                                                                                                  ttGTGTAGATTTATATATTTTGGGCAACTTGTTGTTTATACGACAAACTTTATACCTGAACACGATATATTATAACTCAATGGCATTGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCtt                   <  1:39325/141‑1 (MQ=255)

                                                                                                                     tttAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAag                >  3:173929/1‑141 (MQ=255)

                                                                                                                     tttAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAag                <  2:379122/141‑1 (MQ=255)

                                                                                                                      ttAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAaga               <  1:603749/141‑1 (MQ=255)

                                                                                                                         gATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTg            >  1:591210/1‑141 (MQ=255)

                                                                                                                          aTTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGCACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTgg           <  4:246838/141‑1 (MQ=255)

                                                                                                                           tttATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACACCTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTCAAGATTTATTATGTAGTGATTCTTAAGAGTggg          <  3:163226/141‑1 (MQ=255)

                                                                                                                           tttATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTggg          <  2:371221/141‑1 (MQ=255)

                                                                                                                               tatatTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAg      >  1:351240/1‑141 (MQ=255)

                                                                                                                                atatTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGa     >  4:343559/1‑141 (MQ=255)

                                                                                                                                  atTTTGGGCAACTTGTTGTTTATTCGACAACATTTACACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGaaa   <  2:314684/141‑1 (MQ=255)

                                                                                                                                   ttttGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGAAAt  >  4:32622/1‑141 (MQ=255)

                                                                                                                                   ttttGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGAAAt  <  2:593084/141‑1 (MQ=255)

                                                                                                                                   ttttGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGAAAt  >  1:134198/1‑141 (MQ=255)

TCCTATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGACAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGAAAT  >  USA300TCH1516_ALE/2388729‑2389000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`