BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	638,614	G→T	T14K (ACA→AAA)	pdxK ←	Pyridoxine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	638,614	0	G	T	100.0%	438.6 / NA	119	T14K (ACA→AAA)	pdxK	Pyridoxine kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (67/52); total (67/52)

Only 100 of 123 total aligned reads displayed.

CATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAAT  >  USA300TCH1516_ALE/638477‑638750

cATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTttgt                                                                                                                                        <  1:126678/141‑1 (MQ=255)

cATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTttgt                                                                                                                                        >  2:797042/1‑141 (MQ=255)

cATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTttgt                                                                                                                                        >  1:233548/1‑141 (MQ=255)

    cATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGa                                                                                                                                    >  2:6339/1‑141 (MQ=255)

     aTTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGaa                                                                                                                                   >  1:287762/1‑141 (MQ=255)

      ttGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAAc                                                                                                                                  >  4:292414/1‑141 (MQ=255)

        ggTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCg                                                                                                                                >  4:403040/1‑141 (MQ=255)

        ggTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCg                                                                                                                                <  2:896990/141‑1 (MQ=255)

          tAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGc                                                                                                                              <  1:544798/141‑1 (MQ=255)

          tAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGc                                                                                                                              <  3:320636/141‑1 (MQ=255)

           aaTGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCa                                                                                                                             >  3:82020/1‑141 (MQ=255)

            aTGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCaa                                                                                                                            <  1:588755/141‑1 (MQ=255)

            aTGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCaa                                                                                                                            >  1:135676/1‑141 (MQ=255)

             tGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAAt                                                                                                                           <  1:819296/141‑1 (MQ=255)

             tGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAAt                                                                                                                           >  2:253642/1‑141 (MQ=255)

               gtgtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTg                                                                                                                         >  1:166799/1‑141 (MQ=255)

                 gtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGtt                                                                                                                       <  1:118311/141‑1 (MQ=255)

                 gtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGtt                                                                                                                       <  2:826988/141‑1 (MQ=255)

                  tAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑a                                                                                                                     >  3:52536/1‑141 (MQ=255)

                  tAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑a                                                                                                                     >  4:268627/1‑141 (MQ=255)

                   aaCATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑aa                                                                                                                    >  3:360743/1‑141 (MQ=255)

                    aCATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATAGCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑aaa                                                                                                                   <  1:512439/141‑1 (MQ=255)

                    aCATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑aaa                                                                                                                   >  4:286568/1‑141 (MQ=255)

                      aTCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAAc                                                                                                                 >  3:268052/1‑141 (MQ=255)

                        cGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAActt                                                                                                               >  4:141871/1‑141 (MQ=255)

                        cGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAActt                                                                                                               >  1:114774/1‑141 (MQ=255)

                         gtgtGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAActtt                                                                                                              >  2:118696/1‑141 (MQ=255)

                             gACCATGTATCTTTATCCAGAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCCGCACTTTTGTCAGACCGGGCAATTGTT‑AAAActttcttt                                                                                                          <  2:323786/141‑1 (MQ=255)

                                     aTCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCAt                                                                                                  <  1:406209/141‑1 (MQ=255)

                                      tCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCTAGCACTTTTGTCAGAGCCGGCAATTGTT‑AAAACTTTCTTTAAAGCCAtt                                                                                                 <  3:110458/141‑1 (MQ=255)

                                          tATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGc                                                                                             <  1:671441/141‑1 (MQ=255)

                                          tATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGAAGCCGCTGCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGc                                                                                             <  1:17593/141‑1 (MQ=255)

                                           aTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCt                                                                                            >  2:657534/1‑141 (MQ=255)

                                                tAGTAACGATGGCAGTTAAAGCGACCATGCAATACGTATCTAATTCTTGGAACGTTGTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACt                                                                                       <  1:11676/141‑1 (MQ=255)

                                                 aGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTc                                                                                      >  1:246431/1‑141 (MQ=255)

                                                  gTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTcc                                                                                     >  1:819235/1‑141 (MQ=255)

                                                    aaCGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTa                                                                                   >  2:883723/1‑141 (MQ=255)

                                                     aCGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTAc                                                                                  >  2:449724/1‑141 (MQ=255)

                                                      cGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACa                                                                                 >  3:238545/1‑141 (MQ=255)

                                                        aTGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACata                                                                               >  2:515344/1‑141 (MQ=255)

                                                          ggCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataat                                                                             <  2:167426/141‑1 (MQ=255)

                                                            cAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataa                                                                           >  1:872582/1‑141 (MQ=255)

                                                            cAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataa                                                                           >  2:13154/1‑141 (MQ=255)

                                                            cAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataa                                                                           <  2:630546/141‑1 (MQ=255)

                                                            cAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataa                                                                           >  1:464755/1‑141 (MQ=255)

                                                              gTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataata                                                                         <  1:412365/141‑1 (MQ=255)

                                                               ttAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAatat                                                                        <  3:257036/141‑1 (MQ=255)

                                                                tAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATAtt                                                                       >  1:195345/1‑141 (MQ=255)

                                                                 aaaGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTg                                                                      <  1:407662/141‑1 (MQ=255)

                                                                      gACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCTATTGTT‑AAAACTTTCTTTAAAGCCATTTAGCTTCACTCCTACATAATAATATTGTATtc                                                                 >  4:272383/1‑141 (MQ=255)

                                                                      gACCATGCCATACGTATCTAATCCTTGGAACGTTTTCAAATCTGCTTGAACACCTTCGCCAGCTCCTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTAttt                                                                 >  3:301660/1‑140 (MQ=255)

                                                                      gACCATGAAATACGTATCTAATTCTTGCAACGTTTTCAAATCTGCTGGCATCCCTGCGCCAGGACTTTTGTCAAAACTGGCAACGGTT‑AAGGCTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTAtta                                                                 >  4:322423/1‑140 (MQ=255)

                                                                          aTGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATttatca                                                             >  3:120955/1‑141 (MQ=255)

                                                                           tGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTcatcat                                                            >  1:817392/1‑141 (MQ=255)

                                                                           tGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTcatcat                                                            >  4:159459/1‑141 (MQ=255)

                                                                            gCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTTAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcata                                                           <  2:847630/141‑1 (MQ=255)

                                                                                    tATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAttttt                                                   >  1:410951/1‑141 (MQ=255)

                                                                                    tATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAttttt                                                   <  1:755094/141‑1 (MQ=255)

                                                                                     aTCTAATTCTTGGAACGTTTTCAAATCTGCTTGGATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTa                                                  >  1:502854/1‑141 (MQ=255)

                                                                                     aTCTAATTCTTGGAACGTTTTCAAATCTGCTTGGATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTa                                                  >  1:502768/1‑141 (MQ=255)

                                                                                        tAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAAcc                                               <  4:264609/141‑1 (MQ=255)

                                                                                        tAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCGGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAAcc                                               <  1:44689/141‑1 (MQ=255)

                                                                                           ttCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTaa                                            <  1:913239/141‑1 (MQ=255)

                                                                                            tCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAAt                                           >  4:354154/1‑141 (MQ=255)

                                                                                             cTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAAtt                                          >  1:183710/1‑141 (MQ=255)

                                                                                             cTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAAtt                                          <  2:246442/141‑1 (MQ=255)

                                                                                              ttGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTg                                         <  1:377447/141‑1 (MQ=255)

                                                                                              ttGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTg                                         <  1:898638/141‑1 (MQ=255)

                                                                                               tGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGa                                        >  1:886985/1‑141 (MQ=255)

                                                                                                ggAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTGATTGaa                                       <  1:314756/141‑1 (MQ=255)

                                                                                                  aaCGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGaaaa                                     >  3:147584/1‑141 (MQ=255)

                                                                                                         tCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTaa                              >  4:266889/1‑141 (MQ=255)

                                                                                                             aTCTGCTTGCATACCTGCTCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCa                           <  2:673568/141‑1 (MQ=255)

                                                                                                             aTCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCAt                          <  1:44690/141‑1 (MQ=255)

                                                                                                             aTCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCAt                          >  1:799267/1‑141 (MQ=255)

                                                                                                              tCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCAtt                         <  1:667443/141‑1 (MQ=255)

                                                                                                               cTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTc                        <  2:923882/141‑1 (MQ=255)

                                                                                                               cTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTc                        <  1:738023/141‑1 (MQ=255)

                                                                                                               cTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTc                        <  1:219296/141‑1 (MQ=255)

                                                                                                                 gCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATTTTGTATTCATCATATCATTTTTAACCTAATTGAAAAGTATTAAGCATTTaa                      >  4:100510/1‑141 (MQ=255)

                                                                                                                  cTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAat                     <  1:883723/141‑1 (MQ=255)

                                                                                                                   ttGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTAc                                                                                  <  2:206791/79‑1 (MQ=255)

                                                                                                                   ttGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTAc                                                                                  >  1:206791/1‑79 (MQ=255)

                                                                                                                    tGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAatat                   >  1:720085/1‑141 (MQ=255)

                                                                                                                    tGCATACCTGCGCCAGCACGTTTGTCAGAACCGGCAATTGAG‑AGAACTTGCTGTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACGTAATTGAAAAAGGTTAAGCATTAAatat                   >  2:577702/1‑141 (MQ=255)

                                                                                                                       aTACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtg                >  2:477395/1‑141 (MQ=255)

                                                                                                                        tACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtga               >  1:523089/1‑141 (MQ=255)

                                                                                                                        tACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtga               <  1:3676/141‑1 (MQ=255)

                                                                                                                         aCCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtgat              <  2:21760/141‑1 (MQ=255)

                                                                                                                           cTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtgatga            <  2:894341/141‑1 (MQ=255)

                                                                                                                            tGCGCCAGCACTTTTGTCAGCACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTgatgat           >  3:218728/1‑141 (MQ=255)

                                                                                                                             gcgcCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCAt                                                                                                  <  4:243970/53‑1 (MQ=255)

                                                                                                                             gcgcCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCAt                                                                                                  >  3:243970/1‑53 (MQ=255)

                                                                                                                                ccAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGAttgtt       <  3:348375/141‑1 (MQ=255)

                                                                                                                                ccAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGAttgtt       <  4:388432/141‑1 (MQ=255)

                                                                                                                                  aGCACTTTTGTCAGAACCGGCACTTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGTAAAATATTAAGCATTCAATATTTGATGAttgttta     >  2:920676/1‑139 (MQ=255)

                                                                                                                                  aGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGa     <  2:764604/141‑1 (MQ=255)

                                                                                                                                  aGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGa     >  1:18351/1‑141 (MQ=255)

                                                                                                                                    cACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGaaa   >  3:58826/1‑141 (MQ=255)

                                                                                                                                     aCTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAAt  >  2:854138/1‑141 (MQ=255)

CATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAAT  >  USA300TCH1516_ALE/638477‑638750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`