Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,474,639 C→G 100% A9129P (GCA→CCA)  ebh_1 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,474,6390CG100.0% 85.0 / NA 27A9129P (GCA→CCA) ebh_1Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (16/11);  total (16/11)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGCTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTCATT  >  USA300TCH1516_ALE/1474504‑1474774
                                                                                                                                       |                                                                                                                                       
gACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGTTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGa                                                                                                                                     >  1:220092/1‑140 (MQ=255)
 atttttAATTCTATTTTGTAGTGCATTATTTTTTTCATTAATTTTGTCGATGTTATGTTTACCATTTGTTTTATCAAGTGTTACACGTTCATCAGCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGAt                                                                                                                                    <  1:120959/137‑1 (MQ=255)
         aTCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACttgt                                                                                                                            <  1:147569/140‑1 (MQ=255)
            cATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTCCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGc                                                                                                                         >  2:126494/1‑140 (MQ=255)
                    tgAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTATTTTAGCAATTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGc                                                                                                                 >  1:19699/1‑140 (MQ=255)
                       gCATTGTTTTATGCATTCAATTGGTTGATGTCATGTTTACCTTTTGTTTTAGCTTGTGCAACAGAGTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCAtt                                                                                                              <  1:314234/140‑1 (MQ=255)
                            gTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATAACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCCCTTGTTGCTTATTCGCATTTTGtt                                                                                                         >  2:248391/1‑140 (MQ=255)
                                gaTGCATTCAATTGGTCGATGGCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCaa                                                                                                     <  1:46456/139‑1 (MQ=255)
                                  tGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATCTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATa                                                                                                   <  2:238342/140‑1 (MQ=255)
                                           ttGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCATGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcag                                                                                          >  1:94434/1‑140 (MQ=255)
                                           tgggTTGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcag                                                                                          <  2:126383/138‑1 (MQ=255)
                                            gtgTCTATGTCATGTTTACCATTTGTTTTACCAAGTGCTACACGTTCATCACCCTTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagc                                                                                         <  1:70526/138‑1 (MQ=255)
                                               tCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagcatc                                                                                      >  1:234898/1‑140 (MQ=255)
                                               tCGACGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTAATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagcatc                                                                                      >  2:194334/1‑140 (MQ=255)
                                                                   tGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATGCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGtttcttag                                                                  >  2:237966/1‑135 (MQ=255)
                                                                   tGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTGTGAAGTGGTTGAGCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCACCAATAGCTTGTTTATAAg                                                                  >  2:17972/1‑140 (MQ=255)
                                                                       ttAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCAtt                                                              >  2:22246/1‑140 (MQ=255)
                                                                            aaGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGtt                                                         <  2:234898/140‑1 (MQ=255)
                                                                                gCTACGCGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATc                                                     <  2:291268/140‑1 (MQ=255)
                                                                                  tACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTCAAGCAATACATTTTCAGCATCAGCAAGACCTTGTTTATAAGAATTTAGATTATCTg                                                   >  1:73878/1‑140 (MQ=255)
                                                                                    cacGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGaa                                                 <  2:224094/140‑1 (MQ=255)
                                                                                          catcaCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCt                                           >  1:215226/1‑140 (MQ=255)
                                                                                          catcaCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCt                                           >  2:2810/1‑140 (MQ=255)
                                                                                             tattaTTTTATGCTTGTTTTGTTTTTAAAATAGTTTGGTGTGGTTTCGGTTCTATTTGCTTATTCGCATTTTGTTTGAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtgt                                        <  1:150939/136‑1 (MQ=255)
                                                                                                              tttGCATTTAAAATCTTTTGAGGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCCGCAATAGCTTGTCTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTg                       >  2:219103/1‑140 (MQ=255)
                                                                                                                                tGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTc     >  1:295350/1‑140 (MQ=255)
                                                                                                                                   aGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTCAtt  >  1:214574/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGCTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTCATT  >  USA300TCH1516_ALE/1474504‑1474774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑