BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,481,355	G→T	A6890E (GCA→GAA)	ebh_1 ←	Extracellular matrix‑binding protein ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,481,355	0	G	T	100.0%	569.3 / NA	171	A6890E (GCA→GAA)	ebh_1	Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (77/94); total (77/94)

Only 100 of 180 total aligned reads displayed.

TCAAGTTACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTACCG  >  USA300TCH1516_ALE/1481209‑1481499

tCAAGTTACCCATCGCCGTATTCAGTTCCTGGGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCa                                                                                                                                                 <  2:651785/149‑1 (MQ=255)

  aaGTTACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGGTGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTGGATAATGTTTGGTTTTCAtt                                                                                                                                               >  2:1604812/1‑149 (MQ=255)

  aaGTTACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCAtt                                                                                                                                               >  1:841447/1‑149 (MQ=255)

  aaGTTACCCATCGCTATATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCAtt                                                                                                                                               >  1:78433/1‑149 (MQ=255)

    gTTACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGt                                                                                                                                             >  1:1874819/1‑149 (MQ=255)

    gTTACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGt                                                                                                                                             <  1:1313917/149‑1 (MQ=255)

    gTTACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGAATGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCCAACGTAGATAATGTTTGGTTTTCATTGt                                                                                                                                             >  1:1389968/1‑149 (MQ=255)

      tACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTgaat                                                                                                                                           >  1:1712923/1‑146 (MQ=255)

      tACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGttt                                                                                                                                           <  1:100327/149‑1 (MQ=255)

       tccccTCGCTGTTTGCAGTTCCTGAGCGGTGTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGtttt                                                                                                                                          <  2:35900/148‑1 (MQ=255)

         ccATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTa                                                                                                                                        <  1:325236/149‑1 (MQ=255)

         ccATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTa                                                                                                                                        <  2:490128/149‑1 (MQ=255)

         ccATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTGTCCAGTGACAGCACCTTTTTGTGCATGGTTGAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTa                                                                                                                                        >  2:2312/1‑149 (MQ=255)

          cATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAg                                                                                                                                       >  2:1489512/1‑149 (MQ=255)

                tGTATTCAGTTCCTGCGCGGTTTGAATGGGTTGCGTTACCTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTg                                                                                                                                 <  2:427193/149‑1 (MQ=255)

                tGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTg                                                                                                                                 <  2:149168/149‑1 (MQ=255)

                tGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTg                                                                                                                                 <  1:1106882/149‑1 (MQ=255)

                 gTATTCAGTCCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAAAAAATCATTGGTTTTCATTGTTTTTAGCATCTGt                                                                                                                                <  2:921521/149‑1 (MQ=255)

                      cAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCTTTGTTTTTAGCATCGGTCACtt                                                                                                                           >  2:380547/1‑149 (MQ=255)

                      cAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACtt                                                                                                                           >  2:923744/1‑149 (MQ=255)

                       aGTTCCTGAGCGGTTTGAATGGCTCGCGTGACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACttt                                                                                                                          >  1:1028971/1‑149 (MQ=255)

                        gTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACtttt                                                                                                                         >  1:1630496/1‑149 (MQ=255)

                        gTTCCTGAGCGGTTTGAAGGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACtttt                                                                                                                         <  2:1582699/149‑1 (MQ=255)

                         ttCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTc                                                                                                                        <  1:1501202/149‑1 (MQ=255)

                         ttCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTc                                                                                                                        <  1:1531848/149‑1 (MQ=255)

                          tCCTGGGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCt                                                                                                                       <  2:979148/149‑1 (MQ=255)

                          tCCTGAGCGGTTTGAATGGCGTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCt                                                                                                                       <  1:1066941/149‑1 (MQ=255)

                             tGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATc                                                                                                                    >  2:1343770/1‑149 (MQ=255)

                               aGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑c                                                                                                                 >  1:1721204/1‑149 (MQ=255)

                                  ggTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCAt                                                                                                              <  2:1852742/149‑1 (MQ=255)

                                    tttGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTCGCATCTGTTACTTTTCTATCA‑CCATTc                                                                                                            >  2:1872797/1‑149 (MQ=255)

                                    tttGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTc                                                                                                            >  1:1620279/1‑149 (MQ=255)

                                    tttGAATGGCTTGCGTTACTTCAACTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTc                                                                                                            >  1:1585982/1‑149 (MQ=255)

                                     ttGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCa                                                                                                           <  1:1750180/149‑1 (MQ=255)

                                        aaTGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATAC‑CCATTCAACg                                                                                                        >  2:163641/1‑149 (MQ=255)

                                        aaTGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTGTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCCTCTGTTACTTTTCTATCA‑CCATTCAACg                                                                                                        >  2:818716/1‑149 (MQ=255)

                                            gCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGAGGTTTCCAGTAACAGCACCTTTTTGTGAATGATTTAAGTGATCTAGCGAAGATAATGTTTGGTCTTCATTGTTATTCGCATCTGTTAATTTTCTATCA‑CCCTTAAACGCttt                                                                                                    >  2:260935/1‑148 (MQ=255)

                                                gCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTc                                                                                                <  1:1212895/149‑1 (MQ=255)

                                                gCGTTACTTCAGCTACAGTGTGCGCTCGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTc                                                                                                <  1:635328/149‑1 (MQ=255)

                                                 cGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCg                                                                                               <  2:1069923/149‑1 (MQ=255)

                                                  gTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTGTTGTGCAGTGTTTCAGTTATTTAACGGTGCTAATGTTTGGTTTTCATTGTTTTTAGCATCGGTGACTTTTCTGTCA‑CCATTCAACGCTTGtttatt                                                                                              >  1:37393/1‑146 (MQ=255)

                                                     tcTTCAGCTACGGTGTGCGCTTGATTGATGTTTCCCGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAgt                                                                                           <  2:663315/148‑1 (MQ=255)

                                                      cTTCCGCTAATGTGTTCGTTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAgtt                                                                                          <  2:1620689/149‑1 (MQ=255)

                                                      cTTCAGTTACTGTGTTCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGGTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAgtt                                                                                          <  2:774618/149‑1 (MQ=255)

                                                      cTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTGGCATCTGTTACTTTTCTATCA‑CCGTTCAACGCTTGTTTCGTAgtt                                                                                          >  1:117291/1‑149 (MQ=255)

                                                      cTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTCTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAgtt                                                                                          <  2:1997201/149‑1 (MQ=255)

                                                        tCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGATTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAgttgt                                                                                        <  1:1343770/149‑1 (MQ=255)

                                                         cAGCTACAGTTTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAgttgtt                                                                                       <  2:1268900/149‑1 (MQ=255)

                                                              aCAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGt                                                                                  <  2:1793804/149‑1 (MQ=255)

                                                               cAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGtt                                                                                 <  2:625775/149‑1 (MQ=255)

                                                                aGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTTAGTTATCTAACGTAGATAATGTATGGTTTTCATTGTTTTTAGAATCTGTTACTTTTCTATCA‑ACATTCAACGCTTGTTTGGTAGTTGTTACAGttt                                                                                >  2:1093244/1‑148 (MQ=255)

                                                                 gtgtGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGa                                                                               <  2:179472/149‑1 (MQ=255)

                                                                    tgCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAg                                                                            <  2:1630496/149‑1 (MQ=255)

                                                                            attgatGTTTCCAGTAACAGCCACTTTTGGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCtt                                                                    <  2:503158/149‑1 (MQ=255)

                                                                                    ttCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgt                                                            >  1:1806775/1‑149 (MQ=255)

                                                                                    ttCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgt                                                            >  2:1836162/1‑149 (MQ=255)

                                                                                    ttCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTGTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgt                                                            >  2:1237474/1‑149 (MQ=255)

                                                                                             cAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTc                                                   >  2:1969950/1‑149 (MQ=255)

                                                                                              aGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTATATAATGTTTCGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCCACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCg                                                  <  2:1855871/149‑1 (MQ=255)

                                                                                              aGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCg                                                  <  1:1087189/149‑1 (MQ=255)

                                                                                              aGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCg                                                  >  1:406853/1‑149 (MQ=255)

                                                                                              aGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCg                                                  >  1:654152/1‑149 (MQ=255)

                                                                                              aGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGGTACAGTTGAAGGTGCTGCTTCCACTTGTGCTTGGTTCg                                                  >  1:1402622/1‑149 (MQ=255)

                                                                                                  ccTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CTTTGCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTTATTGGTTCGCAtt                                              <  1:277501/149‑1 (MQ=255)

                                                                                                   cTTTTTGTGCATTGTTTAAGTTTTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCAttt                                             <  1:174513/149‑1 (MQ=255)

                                                                                                   cTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCAttt                                             <  1:1745302/149‑1 (MQ=255)

                                                                                                    tttttGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTAATTA‑TTGGTGATGATGTTAAAGAATCCAGTCAAAGCTTGTTTCGAAGATTTGACGCGGGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTg                                            >  1:959149/1‑149 (MQ=255)

                                                                                                      tttGGGCATTGTTTAAGTTATCTAAGGTAGATAATGTTTGGTTTTCATTGTTTTTAGAATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTa                                          <  2:293756/149‑1 (MQ=255)

                                                                                                       ttGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTAc                                         <  1:2004320/149‑1 (MQ=255)

                                                                                                         gtgCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCa                                       >  2:1383733/1‑149 (MQ=255)

                                                                                                          tgCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGCGATTGGTTCGCATTTTTACCAt                                      >  2:748598/1‑149 (MQ=255)

                                                                                                             tttGCTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTg                                   <  2:1046093/148‑1 (MQ=255)

                                                                                                             aTTGTTTAAGTTATCTAACGTTGATAATGTTTGGTTTTCATTGTTTTTAGCTTCTGTTACTTTTCTCTCA‑CCATTCACCGCTTGTTCCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTg                                   <  2:972763/149‑1 (MQ=255)

                                                                                                             aTTGTATAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTg                                   <  2:67147/149‑1 (MQ=255)

                                                                                                              ttGTTTAAGTTGTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGc                                  >  1:1640339/1‑149 (MQ=255)

                                                                                                              ttGTTTAAGTTATCTAACGTATATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGc                                  <  2:934059/149‑1 (MQ=255)

                                                                                                              ttGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGc                                  >  1:972289/1‑149 (MQ=255)

                                                                                                              ttGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGc                                  <  2:1721204/149‑1 (MQ=255)

                                                                                                                  ttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGa                              >  1:1427636/1‑149 (MQ=255)

                                                                                                                   tAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGAt                             <  1:2312/149‑1 (MQ=255)

                                                                                                                   tAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGAt                             <  2:1407411/149‑1 (MQ=255)

                                                                                                                   tAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGAt                             <  1:1565752/149‑1 (MQ=255)

                                                                                                                      tttATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCTACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaa                          <  2:1918228/148‑1 (MQ=255)

                                                                                                                      gTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaa                          <  2:1818003/149‑1 (MQ=255)

                                                                                                                         aTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaataa                       <  2:1436734/149‑1 (MQ=255)

                                                                                                                          tCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTGTCGTAGTTGTTACAGTTGAAAGTGACGTGACAACTTGTGATTGGTTCGCATAGCGAGCATTTGCTTGATTaataat                      >  1:1445067/1‑149 (MQ=255)

                                                                                                                          tCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCGAGTTTAGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGAGTaataat                      >  1:870230/1‑149 (MQ=255)

                                                                                                                            tAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAatat                    >  2:349213/1‑149 (MQ=255)

                                                                                                                             aaCGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAtt                   <  1:923744/149‑1 (MQ=255)

                                                                                                                             aaCGTAGATAATGTGTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTAAAAAGTGAATGGGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAtt                   <  1:380547/149‑1 (MQ=255)

                                                                                                                               cGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAtttt                 >  1:1723853/1‑149 (MQ=255)

                                                                                                                               cGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAtttt                 >  2:1153810/1‑149 (MQ=255)

                                                                                                                                 tAGATAATGTTTGGTTTTCATTGATTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTcc               >  2:165147/1‑148 (MQ=255)

                                                                                                                                     tAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTCCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTACTAATATTTTcagcag           >  2:1000886/1‑149 (MQ=255)

                                                                                                                                       aTGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTcagcagca         >  1:437191/1‑149 (MQ=255)

                                                                                                                                          tttggttttCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGtt      >  1:813162/1‑149 (MQ=255)

                                                                                                                                          tttggttttCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCCTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGGTTGATTAATAATATTTTCAGCAGCAGtt      >  1:1131112/1‑149 (MQ=255)

                                                                                                                                           ttggttttCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTa     >  1:599234/1‑149 (MQ=255)

                                                                                                                                           ttggttttCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTa     >  1:1839915/1‑149 (MQ=255)

                                                                                                                                             ggttttCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTAcc   <  1:1482099/149‑1 (MQ=255)

TCAAGTTACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCA‑CCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTACCG  >  USA300TCH1516_ALE/1481209‑1481499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`