BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	100.0%	494.5 / NA	142	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (61/81); total (61/81)

Only 100 of 143 total aligned reads displayed.

TCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTTTATT  >  USA300TCH1516_ALE/1646045‑1646334

tCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCttt                                                                                                                                                <  2:448129/149‑1 (MQ=255)

tCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCttt                                                                                                                                                <  2:1050378/149‑1 (MQ=255)

 cAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtttt                                                                                                                                               <  2:789390/149‑1 (MQ=255)

   ataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                             >  1:1496302/1‑149 (MQ=255)

   ataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                             <  2:1856177/149‑1 (MQ=255)

   ataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                             >  1:308613/1‑149 (MQ=255)

     aatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacac                                                                                                                                           >  1:429009/1‑148 (MQ=255)

         ttATCCCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                                       <  1:1577992/149‑1 (MQ=255)

         ttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTATCTATTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                                       <  2:822269/149‑1 (MQ=255)

           aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTAATACTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTa                                                                                                                                     >  2:416993/1‑149 (MQ=255)

              aCTTGCAACACCATGTGGTAATGCACCTCTATGGCCAGCTGCTACAATCGTATCGAATGATGGGCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                                  >  2:1928654/1‑149 (MQ=255)

              aCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                                  >  2:73976/1‑149 (MQ=255)

                   cAACCCCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatatat                                                                                                                             <  1:445114/149‑1 (MQ=255)

                    aaCACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAt‑t                                                                                                                           <  1:7025/149‑1 (MQ=255)

                      cacCATGTGGTAATGCACCTCTATGCCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCa                                                                                                                         <  1:294626/149‑1 (MQ=255)

                       acCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCat                                                                                                                        >  1:144914/1‑149 (MQ=255)

                              ggTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTtc                                                                                                                 <  1:211409/149‑1 (MQ=255)

                              ggTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTtc                                                                                                                 <  1:1414581/149‑1 (MQ=255)

                                tAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTtcat                                                                                                               >  1:1688677/1‑149 (MQ=255)

                                tAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCATTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTtcat                                                                                                               >  1:1768668/1‑149 (MQ=255)

                                   tGCACCTCTATGACCAGATGCTAGAATCGTATCGAATGATGGCTCAACTGCACCTACTTAACTTACTAGGCTGGCTGAGATTGCCTGTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATcaa                                                                                                            >  2:1410941/1‑149 (MQ=255)

                                   tGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTCCAACAGTTAAAATATAT‑TCATATGTTTCATcaa                                                                                                            >  2:938077/1‑149 (MQ=255)

                                   tGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATcaa                                                                                                            >  1:804383/1‑149 (MQ=255)

                                     cACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATcaaca                                                                                                          >  2:1591075/1‑149 (MQ=255)

                                         tctATGACCCGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAGTTCTTTTTCAGTCATGGCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAatat                                                                                                      >  2:659123/1‑149 (MQ=255)

                                         tctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAATATCTATCATATGTTTCATCGACAatat                                                                                                      >  2:1494887/1‑149 (MQ=255)

                                            aTGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAg                                                                                                   >  2:1871801/1‑149 (MQ=255)

                                            aTGACCAGATGCTACAATCGTATCGAATGATGGTCCATCAGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAg                                                                                                   >  1:476808/1‑149 (MQ=255)

                                            aTGACCAGATGCTACAATCGTATCGAATGATGATCCATCTGCTCCTCATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAg                                                                                                   >  1:1283973/1‑149 (MQ=255)

                                               aCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAgctg                                                                                                >  1:1521481/1‑149 (MQ=255)

                                               aCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAACATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAgctg                                                                                                <  2:1345913/149‑1 (MQ=255)

                                                ccAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTGTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACCATATTCgctgc                                                                                               >  1:877416/1‑149 (MQ=255)

                                                    aTGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATCTTTCATCAACAATATTAGCTGCtttt                                                                                           >  2:593088/1‑149 (MQ=255)

                                                      gCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTg                                                                                         <  1:628166/149‑1 (MQ=255)

                                                        tACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACTACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGaa                                                                                       <  1:1135361/149‑1 (MQ=255)

                                                         aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAt                                                                                      >  2:1695663/1‑149 (MQ=255)

                                                          cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAtt                                                                                     >  1:1846641/1‑149 (MQ=255)

                                                            aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCGTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                                   <  2:814007/149‑1 (MQ=255)

                                                              cGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAg                                                                                 >  1:1168679/1‑149 (MQ=255)

                                                                    gAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAttt                                                                           <  1:1546978/149‑1 (MQ=255)

                                                                     aaTGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTc                                                                          >  1:1572633/1‑149 (MQ=255)

                                                                      atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCGTATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAACAAtttag                                                                         >  1:128486/1‑147 (MQ=255)

                                                                       tgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGt                                                                        <  1:1062299/149‑1 (MQ=255)

                                                                        tatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTc                                                                       <  2:236043/148‑1 (MQ=255)

                                                                        gatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTc                                                                       <  2:1279433/149‑1 (MQ=255)

                                                                        gatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTc                                                                       <  2:1630713/149‑1 (MQ=255)

                                                                        gatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTc                                                                       <  1:1060695/149‑1 (MQ=255)

                                                                        gatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTc                                                                       <  1:1078559/149‑1 (MQ=255)

                                                                        gatgGTCCATCTGCTCCTAATTCTACCATTTTTCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTc                                                                       >  2:1936359/1‑149 (MQ=255)

                                                                           ggcccATCTGCTCCTAATTCCACCATTTTGCTTTCCAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑GCAGATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGc                                                                    <  2:1552170/146‑1 (MQ=255)

                                                                              ccATCGTCTTCTAATTCTGGCATTTTGCTTTCTAATATTGCTGTTAGTTGTTTTTCAGTCATGCGTCCTTTTACTACAGCTAAAATATAT‑GCTTATGTTTGATCGACAATATTAGCTGCTTTTTGAAGTAAAGCAATTTCGTCAGctgc                                                                 <  2:217083/149‑4 (MQ=255)

                                                                               cATCTGTCCCTAATTCTATCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCTTCCTTTTACCACCGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCt                                                                <  2:1603686/149‑1 (MQ=255)

                                                                                ctCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt                                                               <  2:466035/148‑1 (MQ=255)

                                                                                aTCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt                                                               <  2:1621437/149‑1 (MQ=255)

                                                                                aTCTGCTCCTAATTCTAGCATGTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAACATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt                                                               <  1:661470/149‑1 (MQ=255)

                                                                                      tCCTTATTCTTGCATTTTGCTTTCTAATATTGTCTTTAATTCCTTTTCCGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATCTGTAACATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                                         <  1:284448/149‑1 (MQ=255)

                                                                                        cTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                                       <  1:1818675/149‑1 (MQ=255)

                                                                                        cTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                                       <  2:1176520/149‑1 (MQ=255)

                                                                                         tAATTCTACCCTTTTGCTTTCTAATCTTGCCTTTAATTCTCTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                                      <  1:367253/149‑1 (MQ=255)

                                                                                         tAATCCCAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                                      <  1:852712/149‑1 (MQ=255)

                                                                                           aTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTATAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAATCAATTTCGTCAGCATCTTTGACGTCTCTa                                                    <  1:1030550/149‑1 (MQ=255)

                                                                                           aTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTa                                                    <  2:295783/149‑1 (MQ=255)

                                                                                           aTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTa                                                    <  2:1164672/149‑1 (MQ=255)

                                                                                           aTTCTAGCATTGTGCGTTCTAATATTGCCTTTATTTCTTTTTCAGTCGTGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAAGATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTa                                                    <  1:1912054/149‑1 (MQ=255)

                                                                                            ttCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTaa                                                   >  2:1035534/1‑149 (MQ=255)

                                                                                            ttCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTaa                                                   <  2:144914/149‑1 (MQ=255)

                                                                                              cTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtt                                                 >  2:953055/1‑149 (MQ=255)

                                                                                                 gCATTTTTCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTa                                              <  1:396866/149‑1 (MQ=255)

                                                                                                  cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTAt                                             <  2:21759/149‑1 (MQ=255)

                                                                                                    ttttGCTTTCTTACATTGCCTTTAATTCTTTTTCAGTCATGCATCAAAATCCGGCAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCt                                           <  1:556241/149‑1 (MQ=255)

                                                                                                      ttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATGTAc                                         <  2:521468/149‑1 (MQ=255)

                                                                                                          tttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACCGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTAAAGTa                                     >  2:840936/1‑149 (MQ=255)

                                                                                                           ttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAt                                    >  2:1966065/1‑149 (MQ=255)

                                                                                                              tAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAg                                 <  2:761042/149‑1 (MQ=255)

                                                                                                                 tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaaa                              >  1:796596/1‑149 (MQ=255)

                                                                                                                   tcgacTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg                            <  2:1913694/145‑1 (MQ=255)

                                                                                                                      cccttAATTCTTTTTCAAACATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtt                         <  1:1606167/146‑1 (MQ=255)

                                                                                                                      ccTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtt                         <  2:1085215/149‑1 (MQ=255)

                                                                                                                       cTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtta                        <  2:1521481/149‑1 (MQ=255)

                                                                                                                       cTTTAATTCTTTAACAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACCGTATTTGCTATGCtta                        <  2:1677920/149‑1 (MQ=255)

                                                                                                                          tAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatta                     >  1:709928/1‑149 (MQ=255)

                                                                                                                          tAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatta                     >  1:1883774/1‑149 (MQ=255)

                                                                                                                              tCTTTTTCAGTTATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTTATTTTATCTACAGTATTAGAAATGCTTATTAATGa                 <  2:1108598/149‑1 (MQ=255)

                                                                                                                               cTTTTTCAGTCATGCCTCCTTTTACAACAGTTAACATATAT‑TCAAATGATTCGTCTGCAAGATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGat                <  1:57922/149‑1 (MQ=255)

                                                                                                                                tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGata               >  1:1784632/1‑149 (MQ=255)

                                                                                                                                tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGata               >  1:517815/1‑149 (MQ=255)

                                                                                                                                 ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGatat              >  2:634972/1‑149 (MQ=255)

                                                                                                                                  tttCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGAtata             <  1:89973/149‑1 (MQ=255)

                                                                                                                                   ttCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGgtattg            >  2:700009/1‑147 (MQ=255)

                                                                                                                                   ttCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAc            >  2:1250852/1‑149 (MQ=255)

                                                                                                                                    tCAGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACg           >  1:1838337/1‑149 (MQ=255)

                                                                                                                                      aGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCACTTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGc         <  2:1605811/149‑1 (MQ=255)

                                                                                                                                      aGTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGc         <  2:239419/149‑1 (MQ=255)

                                                                                                                                       gTCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCt        >  2:1350331/1‑149 (MQ=255)

                                                                                                                                        tCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCtt       >  2:1200396/1‑149 (MQ=255)

                                                                                                                                        tCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCtt       <  2:194487/149‑1 (MQ=255)

                                                                                                                                        tCATGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCtt       <  1:1726378/149‑1 (MQ=255)

                                                                                                                                           tGCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTttta    >  1:1065399/1‑149 (MQ=255)

                                                                                                                                            gCCTCCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTtttat   <  2:1846641/149‑1 (MQ=255)

                                                                                                                                             cctcctTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTtttatt  <  2:292224/149‑1 (MQ=255)

TCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATAT‑TCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTTTATT  >  USA300TCH1516_ALE/1646045‑1646334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`