BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,621,781	A→C	L297* (TTA→TGA)	USA300HOU_RS13520 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,621,781	0	A	C	98.8%	592.8 / ‑5.4	169	L297* (TTA→TGA)	USA300HOU_RS13520	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (85/82); total (86/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 170 total aligned reads displayed.

TACTAAATACTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATAAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCTTTTAA  >  USA300TCH1516_ALE/2621635‑2621926

    aaaTACTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCaa                                                                                                                                             >  1:1065081/1‑149 (MQ=255)

     aaTACTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACATGGAAACACGTTAATAATCGATCATCCAAt                                                                                                                                            >  1:295918/1‑149 (MQ=255)

     aaTACTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGCCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGACACACATTAATAATCGATCAGCCCAt                                                                                                                                            >  1:145951/1‑149 (MQ=255)

       tccTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAACGTTGCACCTATATATACTATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTc                                                                                                                                          <  1:58959/147‑1 (MQ=255)

       tACTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATATTGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTc                                                                                                                                          <  1:58781/149‑1 (MQ=255)

       tACTTTCCCCATCAAATGATCTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTc                                                                                                                                          <  1:1108156/149‑1 (MQ=255)

        aCTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTcc                                                                                                                                         <  1:1973756/149‑1 (MQ=255)

        aCTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTcc                                                                                                                                         <  1:1576234/149‑1 (MQ=255)

            tCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACCTTAATAATCGATCAGCCAATTCCCCaa                                                                                                                                     <  1:1491834/149‑1 (MQ=255)

             ccccATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAATCCCTCTATAGATTGTCCAAGACCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAAACGATCAGCCAATTCCCCaaa                                                                                                                                    >  2:1174547/1‑149 (MQ=255)

                  tCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTaaa                                                                                                                               <  2:1187504/149‑1 (MQ=255)

                  tCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAAGAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCACTTCCCCAAACTaaa                                                                                                                               <  1:798231/149‑1 (MQ=255)

                     aaTTATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAaca                                                                                                                            <  2:1832012/149‑1 (MQ=255)

                     aaTGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCATACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAaca                                                                                                                            <  1:1370055/149‑1 (MQ=255)

                       tGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAGTTCCCCAAACTAAAACAcc                                                                                                                          <  2:883929/149‑1 (MQ=255)

                       tGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACAcc                                                                                                                          <  2:255980/149‑1 (MQ=255)

                              gCGTTTTTATTTGAATGGCAACTGGTTTTGTTAGTCCCGCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTa                                                                                                                   <  2:257317/149‑1 (MQ=255)

                              gCGTTTTGATTTGAATGGCAACTGATCTTGTTAGTCCCTCCATAGATTGTCCAAGTCCCTATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATCCCCCAAACTAAAACACCAATACTa                                                                                                                   <  2:1588702/149‑1 (MQ=255)

                                    ttATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTa                                                                                                             >  1:817533/1‑149 (MQ=255)

                                     tATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTaa                                                                                                            >  1:597858/1‑149 (MQ=255)

                                        ttGAATGGCAACTGAGCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAACAATATTTCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGa                                                                                                         <  2:1204066/149‑1 (MQ=255)

                                         tGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGAt                                                                                                        >  1:383971/1‑149 (MQ=255)

                                              ggCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATc                                                                                                   >  1:316417/1‑149 (MQ=255)

                                               gCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCg                                                                                                  <  2:605545/149‑1 (MQ=255)

                                                           gTTAGTCCCTCTTTAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAg                                                                                      <  2:365088/149‑1 (MQ=255)

                                                            ttAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGc                                                                                     >  2:683056/1‑149 (MQ=255)

                                                              aGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATACCGCCACACTTGGAAACACATTAATAAGCGATCAGCCAAGTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCaa                                                                                   <  1:1065004/149‑1 (MQ=255)

                                                                   ctctATAGATTGTCCACGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCATCCAATGCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCa                                                                              <  1:1849794/149‑1 (MQ=255)

                                                                    cctATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAACCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCAt                                                                             <  2:906897/148‑1 (MQ=255)

                                                                        taGATTGTCCAAGTCCCAATAATGTTGCACCTATGTATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATc                                                                         <  1:207994/149‑1 (MQ=255)

                                                                        taGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATc                                                                         <  1:1953765/149‑1 (MQ=255)

                                                                        taGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATc                                                                         <  1:1048314/149‑1 (MQ=255)

                                                                        aaGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATc                                                                         <  2:1526385/148‑1 (MQ=255)

                                                                         aGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCa                                                                        >  1:1619725/1‑149 (MQ=255)

                                                                          gATTGTCCAAGTCCCAATAATTTTGCACCTATATATAATATCGCCAGAGTTGTAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTTACAGCAAACTCATAATCaa                                                                       <  2:560080/149‑1 (MQ=255)

                                                                            ttGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTTACAGCAAACCCATAATCAATg                                                                     >  1:424577/1‑149 (MQ=255)

                                                                            ttGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATg                                                                     >  1:1826123/1‑149 (MQ=255)

                                                                              gTCCAAGTCCCAATAATGTTGCACCTATATATCATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAAGTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGAc                                                                   <  2:1978829/149‑1 (MQ=255)

                                                                               tCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTACCACACCAAGACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACa                                                                  <  1:1885303/149‑1 (MQ=255)

                                                                                ccAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACAt                                                                 >  1:1029909/1‑149 (MQ=255)

                                                                                  aaGTCCCAATAATGTTGCACCTATATCTAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAACAAACCCATAATCAATAACAtaa                                                               >  2:468180/1‑147 (MQ=255)

                                                                                  aaGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAAGAGATTAATAGTCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGCATGAATAGCCATAATCAATAACATta                                                               >  1:1533950/1‑149 (MQ=255)

                                                                                   aGTCCCTATAATGTTGCAACTAAATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATtaa                                                              >  2:1355557/1‑149 (MQ=255)

                                                                                   aGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATtaa                                                              >  2:1670595/1‑149 (MQ=255)

                                                                                   aGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATtaa                                                              <  2:1784115/149‑1 (MQ=255)

                                                                                      cccAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATtaataa                                                           <  2:383971/149‑1 (MQ=255)

                                                                                      cccAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATtaataa                                                           >  1:1775363/1‑149 (MQ=255)

                                                                                       ccAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATaaa                                                          >  2:1728261/1‑149 (MQ=255)

                                                                                        cAATAATGTTGCACCTATATATAATATCGCCACCCTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAg                                                         >  2:1417243/1‑149 (MQ=255)

                                                                                          ataatGTTGCACCTATCTATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCACTGACATtaataacacc                                                       >  2:45416/1‑145 (MQ=255)

                                                                                          ataatGTTGCACATATATATAATAGCGTCACACTTGGAAACACATTAATAATCGATAAGACAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGTTTAATCGCATTTATGACAGCAAACCCGTAATCAATGTCATTAATaaacag                                                       >  2:731860/1‑146 (MQ=255)

                                                                                           aaatGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATg                                                      <  1:1183143/148‑1 (MQ=255)

                                                                                            aatGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGc                                                     <  1:1485429/149‑1 (MQ=255)

                                                                                            aatGTTGCACCGATCTATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGc                                                     <  2:641588/149‑1 (MQ=255)

                                                                                                    aCCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCGAATTCCCCAAGCTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTTTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTa                                             <  2:201544/149‑1 (MQ=255)

                                                                                                      cTATATATAATATCGCCACACTTGGAAACACATTAATAATCTATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTaaa                                           <  2:1919851/149‑1 (MQ=255)

                                                                                                      cTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTaaa                                           <  2:211877/149‑1 (MQ=255)

                                                                                                          atataATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAGGATGCTATACCTAAACatg                                       >  1:489063/1‑149 (MQ=255)

                                                                                                             taataTCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGCCAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACatgatg                                    >  1:1530185/1‑149 (MQ=255)

                                                                                                             taataTCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACatgatg                                    <  1:1728261/149‑1 (MQ=255)

                                                                                                             taataTCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAAAACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACatgatg                                    >  1:171460/1‑149 (MQ=255)

                                                                                                             taataTCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAAATAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAAAatgatg                                    >  1:933221/1‑149 (MQ=255)

                                                                                                                tatCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATACAGATACTATACCTAAACATGATGTAg                                 >  2:1263699/1‑149 (MQ=255)

                                                                                                                tatCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTaa                                 >  1:566859/1‑148 (MQ=255)

                                                                                                                tatCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAg                                 >  2:1264321/1‑149 (MQ=255)

                                                                                                                tatCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAAATGCTATACCAAAACATGATGTAg                                 >  2:295962/1‑149 (MQ=255)

                                                                                                                 atCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGc                                >  2:984257/1‑149 (MQ=255)

                                                                                                                 atCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGc                                >  1:893794/1‑149 (MQ=255)

                                                                                                                  tCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGcc                               >  1:891131/1‑148 (MQ=255)

                                                                                                                  tCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGca                               >  2:5720/1‑148 (MQ=255)

                                                                                                                  tCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCt                               >  2:1110182/1‑149 (MQ=255)

                                                                                                                   cGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGCTTAATCGCTATTCTGACAGCCACATGAGCAGCAATGACCATAACAAAGATGCAATACCTAAACATGATGTAGCTa                              >  1:454377/1‑149 (MQ=255)

                                                                                                                   cGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTa                              >  1:1951511/1‑149 (MQ=255)

                                                                                                                   cGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTa                              >  1:1227726/1‑149 (MQ=255)

                                                                                                                   cGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTa                              >  2:469610/1‑149 (MQ=255)

                                                                                                                   cGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATAATGTAGCTa                              >  1:1394646/1‑149 (MQ=255)

                                                                                                                     ccACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGt                            >  2:1460593/1‑149 (MQ=255)

                                                                                                                      cacaCTTTGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTc                           <  1:1331764/149‑1 (MQ=255)

                                                                                                                      cacaCTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTc                           <  2:215905/149‑1 (MQ=255)

                                                                                                                        cccTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCa                         <  2:1024782/147‑1 (MQ=255)

                                                                                                                        cacTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCa                         <  2:1219193/149‑1 (MQ=255)

                                                                                                                         acTTGTAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTGTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCat                        <  1:1397584/149‑1 (MQ=255)

                                                                                                                             ggAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATac                    >  1:1883990/1‑149 (MQ=255)

                                                                                                                              gAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACAAAATTTAAAGATCAATCGCCTCTCTGACAGCAAACCCATAATCAATGACATCAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATacc                   >  2:1162496/1‑148 (MQ=255)

                                                                                                                               aaaCACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATacac                  <  1:226430/149‑1 (MQ=255)

                                                                                                                                    caTTAATAATGGATCAGCCAATTCCTCAAACTAGAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACACATAATCAATGACATTAATAAAGATGCTATACCTAAAAATGATGTAGTTAGTACATATACATTAACg             >  2:726310/1‑149 (MQ=255)

                                                                                                                                    caTTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGGCCATATACACCAACg             >  1:663759/1‑149 (MQ=255)

                                                                                                                                    caTTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATGAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACg             >  2:1475024/1‑149 (MQ=255)

                                                                                                                                       taataaTCGACCAGCCAATTCCACAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGccc          <  2:947614/149‑1 (MQ=255)

                                                                                                                                         ataatCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCAATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGATAGTCCATATCCACCAACGCCctc        >  2:15356/1‑149 (MQ=255)

                                                                                                                                          aaatCGACCAGCCAATTCTCCAAACAAAAACACCAATACTAAATTTAAAGATTAATCGCTTTACTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCctct       <  1:203016/148‑1 (MQ=255)

                                                                                                                                            atCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATTACATGAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCttt     <  1:60512/149‑1 (MQ=255)

                                                                                                                                            atCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTGAACATGATGTAGCTAGTCCATATACACCAACGCCCTCttt     <  1:1434451/149‑1 (MQ=255)

                                                                                                                                            atCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCttt     <  1:965682/149‑1 (MQ=255)

                                                                                                                                            atCGATCAGCCAATTCCACAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCttt     <  1:837381/149‑1 (MQ=255)

                                                                                                                                             tCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCtttt    >  2:1834652/1‑149 (MQ=255)

                                                                                                                                              cGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAACCATGCTGTAGCTAGTCCATATACGCCATCGCCCTCTTTTa   <  2:1676336/149‑1 (MQ=255)

                                                                                                                                              cGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCTTTTa   <  2:852289/149‑1 (MQ=255)

                                                                                                                                              cGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCTTTTa   <  1:1030929/149‑1 (MQ=255)

                                                                                                                                               gATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCTTTTaa  <  2:1232617/149‑1 (MQ=255)

TACTAAATACTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATAAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCTTTTAA  >  USA300TCH1516_ALE/2621635‑2621926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`