BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,623,244	G→A	P126L (CCA→CTA)	USA300TCH1516_02501 ←	Putative surface protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,623,244	0	G	A	96.7%	562.2 / NA	186	P126L (CCA→CTA)	USA300TCH1516_02501	Putative surface protein
Reads supporting (aligned to +/- strand): ref base G (3/3); new base A (97/79); total (102/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

AATTATACTACTAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTGGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACAATCT  >  USA300TCH1516_ALE/2623099‑2623388

  ttATACTACTAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCa                                                                                                                                             <  1:417643/149‑1 (MQ=255)

     tactacTAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCAttt                                                                                                                                          >  1:197249/1‑149 (MQ=255)

     tactacTAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCAttt                                                                                                                                          >  1:506834/1‑149 (MQ=255)

      actactAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATTAACTAGGCCATTTg                                                                                                                                         >  1:382706/1‑149 (MQ=255)

      actactAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTCGGCCATTTg                                                                                                                                         >  1:647686/1‑149 (MQ=255)

        tactaAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGt                                                                                                                                       <  1:1204159/149‑1 (MQ=255)

         actaAAGATCAAACCTTTTTTTTTTTTTTTTATACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTCGATTTTTTAACTTTATCATTTTTTTCCTTTTTATGAACTAGGCCATTTGGtt                                                                                                                                      >  2:427124/1‑149 (MQ=255)

                    aaCCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTGGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCt                                                                                                                           >  1:543584/1‑149 (MQ=255)

                    aaCCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCt                                                                                                                           <  2:370457/149‑1 (MQ=255)

                    aaCCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCt                                                                                                                           >  2:101522/1‑149 (MQ=255)

                       cTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTc                                                                                                                        <  2:1386725/149‑1 (MQ=255)

                       cTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTc                                                                                                                        <  1:1663356/149‑1 (MQ=255)

                        tttttGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCg                                                                                                                       >  2:1027793/1‑149 (MQ=255)

                                 gCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTg                                                                                                              >  1:1220941/1‑149 (MQ=255)

                                  cTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTgg                                                                                                             >  1:1049182/1‑149 (MQ=255)

                                     tCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCGTTGATTTAACTCGGCCCTTCGGTTTTGCTCTGTCTTTCGATAATCCTGGAtt                                                                                                          >  1:265197/1‑149 (MQ=255)

                                       tAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGGTAAAGATTCTTTAGCAATTTTAGATTTTTTAACTGTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGt                                                                                                        >  1:1178083/1‑149 (MQ=255)

                                       tAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGt                                                                                                        >  2:192923/1‑149 (MQ=255)

                                       tAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGt                                                                                                        <  2:1997140/149‑1 (MQ=255)

                                       tAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGt                                                                                                        <  1:608070/149‑1 (MQ=255)

                                        aaaCCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGtt                                                                                                       <  1:14033/149‑1 (MQ=255)

                                        aaaCCTGTTTTTGGTAATTCTGCTCGTGTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGtt                                                                                                       <  1:1001377/149‑1 (MQ=255)

                                         aaCCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTa                                                                                                      >  1:394390/1‑149 (MQ=255)

                                         aaCCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTa                                                                                                      >  2:1331835/1‑149 (MQ=255)

                                          aCCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGTATGAACTAGGCCTTTTGGTTTTGCTCTGTCTTTCGATAATCTTGGATTGTGAg                                                                                                     >  2:1347088/1‑149 (MQ=255)

                                             tGTTTTTGGTAATTCTGCTCGTTTTTTCTTTTGATTAGCTACTGATTCTTTAGCTATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTTTCTTTCGTTAATCTTGGTTTTTTAggtt                                                                                                  >  2:1273127/1‑147 (MQ=255)

                                              gTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGAtt                                                                                                 <  1:1787069/149‑1 (MQ=255)

                                                ttttGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCCTTGAATGAACTGGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTa                                                                                               <  2:916227/149‑1 (MQ=255)

                                                ttttGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTa                                                                                               >  1:1821410/1‑149 (MQ=255)

                                                  ttGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACt                                                                                             <  1:631475/149‑1 (MQ=255)

                                                    ggTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACGGATTCGTGTGCAAGTGTGGAGTTTCTAACTTGATCATTTGTAGCCATTGAATGAACTAGGCCTTTTGGTTTTGCTCTGTCTGTCGATAATCCTGTATTGTTAGGATTTACTgg                                                                                           <  2:791928/149‑1 (MQ=255)

                                                      tAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGc                                                                                         <  1:1869209/149‑1 (MQ=255)

                                                      tAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGc                                                                                         <  1:699130/149‑1 (MQ=255)

                                                        aTTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCa                                                                                       >  1:988460/1‑149 (MQ=255)

                                                         ttCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCAc                                                                                      >  2:701848/1‑149 (MQ=255)

                                                         ttCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATACATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCAc                                                                                      >  2:683768/1‑149 (MQ=255)

                                                          tCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACt                                                                                     >  2:1744165/1‑149 (MQ=255)

                                                           cTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGTGGCAcgt                                                                                    >  2:146556/1‑147 (MQ=255)

                                                             gCTCGTTTTTTTTTTTTTTTTTTTACTGATTCTTTAGCAATTTTAGATTTTTTAATTTTATTATTTTTATCAATTGAATGGACTGGTCCATTTGGTTTTGTTTTTTCTTTCGATATTCTTGTATTGTTAGGTTTTACTGGGctgcttcg                                                                                  >  2:495511/1‑142 (MQ=255)

                                                              cTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGa                                                                                 <  1:427124/149‑1 (MQ=255)

                                                                   tttttCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGCTTGTTAGGATTTACTGGGCCACTTGGATGAGt                                                                            >  1:1236181/1‑149 (MQ=255)

                                                                       tttctGGATGAGCTACCGATTCTTTAAAAAGGCAGGATGTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGt                                                                        <  1:452891/147‑1 (MQ=255)

                                                                       tctcTTGATTAGCTACTGATTCTTTAGCAATTGTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTGTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGt                                                                        <  1:1339449/149‑1 (MQ=255)

                                                                         tctTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCt                                                                      >  2:1675162/1‑149 (MQ=255)

                                                                         tctTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCt                                                                      >  1:1480426/1‑149 (MQ=255)

                                                                          ctcgATTAACTACTGATTCTTGAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTTATTGAACTAAGCCAGTTGGTTATGCTCTGTCTTTCGATAGTTCTGTAGTGTTAGGTTTTACTGGGCCACTAGGTGGAGGTGTTCTg                                                                     >  2:344053/4‑149 (MQ=255)

                                                                          ctTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTg                                                                     >  1:1505589/1‑149 (MQ=255)

                                                                          ctTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTg                                                                     >  2:1165930/1‑149 (MQ=255)

                                                                          ctTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTGTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTATTGTGCCACTTGGATGAGTTtgtgtg                                                                     >  1:347364/1‑149 (MQ=255)

                                                                            ttaTTAGCTACTTATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTCGGCCACTTGGATGAGTTGGTCTGCt                                                                   <  2:405486/147‑1 (MQ=255)

                                                                            tGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTCGGCCACTTGGATGAGTTGGTCTGCt                                                                   <  2:1859353/149‑1 (MQ=255)

                                                                                tggcgaCTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGc                                                               <  2:1192877/144‑1 (MQ=255)

                                                                                  gCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCtt                                                             >  1:1420552/1‑149 (MQ=255)

                                                                                   cTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTCCTCGGCTtc                                                            >  1:1538656/1‑149 (MQ=255)

                                                                                    tACTGATTCTTTAGCAATTTTCGATTTTTTAAATTTAACATTTTTATCCATTCAATGAACTGGGCTATTTCGGTGTGCTCTTTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTtct                                                           <  2:669073/149‑1 (MQ=255)

                                                                                        gATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTgg                                                       >  2:1939559/1‑149 (MQ=255)

                                                                                         actCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTggg                                                      >  1:85585/3‑149 (MQ=255)

                                                                                          ttCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGt                                                     >  1:1710495/1‑149 (MQ=255)

                                                                                          ttCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGt                                                     <  1:584726/149‑1 (MQ=255)

                                                                                            cTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGttt                                                   <  2:1611058/149‑1 (MQ=255)

                                                                                            cTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGttt                                                   <  2:1049182/149‑1 (MQ=255)

                                                                                            cTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGttt                                                   <  2:1554237/149‑1 (MQ=255)

                                                                                              ttAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTc                                                 >  1:702333/1‑149 (MQ=255)

                                                                                                  cAATTTTAGATTTTTTAACTTTATCATTTTTCTCAACTAAATGAACTGGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAggg                                             >  2:830795/1‑148 (MQ=255)

                                                                                                  cAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGt                                             >  1:228191/1‑149 (MQ=255)

                                                                                                     ttttAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCt                                          <  1:193105/149‑1 (MQ=255)

                                                                                                      tttAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCtt                                         <  2:359222/149‑1 (MQ=255)

                                                                                                            ttttttATCTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGAt                                   >  2:1302534/1‑149 (MQ=255)

                                                                                                            ttttttAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGAt                                   <  1:176955/149‑1 (MQ=255)

                                                                                                            ttttttAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGAt                                   >  1:1579735/1‑149 (MQ=255)

                                                                                                            ttttttAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGCACACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGAt                                   >  1:56907/1‑149 (MQ=255)

                                                                                                              ttttAACTTTATCATTTTTATCAATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGTCCCCTTGGATGAGTTGGTCTGCTCGGCGTCTCTGGGTTTTCAGGTCCTTTTGTATCt                                 >  2:1778295/1‑149 (MQ=255)

                                                                                                                ttAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCATGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGGTCttt                               >  2:948853/1‑149 (MQ=255)

                                                                                                                  aaCTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTg                             <  1:1067103/149‑1 (MQ=255)

                                                                                                                   aCTTTATCATTTTTATCCATTGAATTAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTgg                            <  1:683768/149‑1 (MQ=255)

                                                                                                                         tCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctc                      <  1:101522/149‑1 (MQ=255)

                                                                                                                         tCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctc                      >  1:1107965/1‑149 (MQ=255)

                                                                                                                         acagttttATCCATTGAATGAACTAGGCCACTTGGTTTTGCGCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACGGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctc                      <  1:844403/145‑1 (MQ=255)

                                                                                                                          cATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctct                     <  1:1891835/149‑1 (MQ=255)

                                                                                                                           aTTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctctc                    >  2:1596276/1‑149 (MQ=255)

                                                                                                                           aTTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGAATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctctc                    >  2:529737/1‑149 (MQ=255)

                                                                                                                               ttATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCAcc                >  1:661012/1‑149 (MQ=255)

                                                                                                                                  tCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGaa             >  2:1967457/1‑149 (MQ=255)

                                                                                                                                    cATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTCCTCTGGGTTTTCAGGTCCTTTTTGACCTTTTGGTTTCTCTCCACCGAAct           <  1:1113713/149‑1 (MQ=255)

                                                                                                                                    cATTGAATGAACTAGGCCATCTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAAct           <  2:312452/149‑1 (MQ=255)

                                                                                                                                      ttGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGCTTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAActct         >  1:1312220/1‑149 (MQ=255)

                                                                                                                                      ttGAATGAACTAGAACATTTGGTTTTGATGTGTCTATGGGTAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAActct         >  1:1471981/1‑149 (MQ=255)

                                                                                                                                       tgaatgaaCTCAGCCATTTGGGTTTGCTCTGTCTTTCGATAATCCTGAATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGCTTTCTCTCCTCCTAActttt        <  2:239273/149‑4 (MQ=255)

                                                                                                                                       tgaatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTa        <  1:924240/149‑1 (MQ=255)

                                                                                                                                       tgaatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTa        <  2:897203/149‑1 (MQ=255)

                                                                                                                                       tgaatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTa        <  1:1961277/149‑1 (MQ=255)

                                                                                                                                       tgaatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTa        <  1:1939559/149‑1 (MQ=255)

                                                                                                                                       tgaatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTa        <  1:1740054/149‑1 (MQ=255)

                                                                                                                                        ggatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTGCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGTCTTCTCTGGGTTTTCAGGGCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTAc       <  1:775288/147‑1 (MQ=255)

                                                                                                                                         aatgaaCTAGGCCATTTGGTTTTGCTCTGGCTTTCGATAATCCTGGATCGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCCTCTCTGGGTTATCAGGTCCTGTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACa      >  2:1689767/1‑149 (MQ=255)

                                                                                                                                         aatgaaCTAGGCCATTGGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTATCTCCACCGCACTATACa      >  1:735496/1‑149 (MQ=255)

                                                                                                                                          atgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACaa     >  1:325650/1‑149 (MQ=255)

                                                                                                                                          atgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACaa     <  1:35603/149‑1 (MQ=255)

                                                                                                                                           tgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACAAt    <  2:647686/149‑1 (MQ=255)

                                                                                                                                           tgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGACACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACAAt    <  1:1921528/149‑1 (MQ=255)

AATTATACTACTAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTGGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACAATCT  >  USA300TCH1516_ALE/2623099‑2623388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`