BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	638,614	G→T	T14K (ACA→AAA)	pdxK ←	Pyridoxine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	638,614	0	G	T	98.5%	654.0 / ‑5.1	203	T14K (ACA→AAA)	pdxK	Pyridoxine kinase
Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (80/120); total (81/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

TTTCAAATACATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAATGAATCATTC  >  USA300TCH1516_ALE/638468‑638759

    aaaTACATCCATTGGTAGTGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAg                                                                                                                                             >  2:630050/1‑149 (MQ=255)

       tACATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACTTGCGCCAGCACTTTTGTCAGAAc                                                                                                                                          >  2:1301370/1‑149 (MQ=255)

        aCATCCATTGGTAATGGTGTAACATCCTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAAcc                                                                                                                                         <  1:40849/149‑1 (MQ=255)

           tCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGc                                                                                                                                      >  1:337170/1‑149 (MQ=255)

              aTTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAAt                                                                                                                                   <  2:1866660/149‑1 (MQ=255)

               ttGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGACATACGTATCTAATTATTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAAtt                                                                                                                                  >  1:1397518/1‑149 (MQ=255)

                tGGTAATGGTGTAATATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTg                                                                                                                                 <  2:617211/149‑1 (MQ=255)

                tGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTg                                                                                                                                 <  1:1848810/149‑1 (MQ=255)

                tGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTg                                                                                                                                 <  2:1453967/149‑1 (MQ=255)

                 ggTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTATTTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGt                                                                                                                                <  1:1229084/149‑1 (MQ=255)

                 ggTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGt                                                                                                                                <  1:1614190/149‑1 (MQ=255)

                  gTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGtt                                                                                                                               >  2:1026110/1‑149 (MQ=255)

                   tAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTa                                                                                                                              >  1:1010943/1‑149 (MQ=255)

                      tGGTGTAACATCGCGTGACCATGTATCTTTCTTGTCAGATACGATGGCCGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTAGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTaaaa                                                                                                                           >  1:1472132/1‑149 (MQ=255)

                       ggTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAAc                                                                                                                          >  1:1181964/1‑149 (MQ=255)

                       ggTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAAc                                                                                                                          >  1:1608425/1‑149 (MQ=255)

                         tgtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCATATCTTTTTGCATACCAGCGCCAGCACTTTTATCACAACCGGCAATTGTTGAAAcct                                                                                                                        >  2:659469/1‑147 (MQ=255)

                          gtagcctCGTGTGACCACTTATCTTTATCCATAGTAACGATGGCAGTTAAATCGACCATGCCATACGTAGCTAATTCTTGGAACGTTTTCAAATCGTCTTGCATACCTGCGCCAGCGCCTTTGTCAGAACCGGCAATTGTTAAAActtt                                                                                                                       <  2:637747/143‑1 (MQ=255)

                          gtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCGGCCCTTTTGTCAGAACCGGCAATTGTTAAACcttt                                                                                                                       >  1:190202/1‑149 (MQ=255)

                          gtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActtt                                                                                                                       >  2:615733/1‑149 (MQ=255)

                          gtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATAGCAGTTAAAGCAACCATGCCATACGTATCTAATGCTTAGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTTTCAGAACCGGGAATTGGTAAAActtt                                                                                                                       >  2:562463/1‑149 (MQ=255)

                            aaCATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActttct                                                                                                                     <  1:1805084/149‑1 (MQ=255)

                            aaCATCGTCTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAGCCGGCAATTGTTAAAActttct                                                                                                                     <  2:1367600/149‑1 (MQ=255)

                               aTCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTa                                                                                                                  <  1:377946/149‑1 (MQ=255)

                                    gtgACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGcc                                                                                                             >  2:12220/1‑149 (MQ=255)

                                    gtgACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGAACATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCCGAACCGGCAATTGTCAAAACCTTCTTTAAAGcc                                                                                                             >  2:1827649/1‑149 (MQ=255)

                                    gtgACCATGTATCTTGATCCATAGTAACAATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTCTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGcc                                                                                                             >  1:1262658/1‑149 (MQ=255)

                                      gACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCAt                                                                                                           <  1:1156453/149‑1 (MQ=255)

                                       aCCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAACCCAtt                                                                                                          >  2:747117/1‑149 (MQ=255)

                                              aTCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGGACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTc                                                                                                   <  2:29435/149‑1 (MQ=255)

                                              aTCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTc                                                                                                   <  1:1820228/149‑1 (MQ=255)

                                              aTCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTc                                                                                                   <  1:635672/149‑1 (MQ=255)

                                              aTCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTc                                                                                                   >  2:1687124/1‑149 (MQ=255)

                                              aTCTTTACCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTTAAGCCATTGAGCTTc                                                                                                   <  2:1773772/149‑1 (MQ=255)

                                                atttATCCTTAGTTACGAAGCCAGTTAAAGCGACCATGCCATACGAATCTGATTCTTGGAACGTTTTCAACTCTGCTTGCATTCCTGCGCCACCGCTTGACGCCGAACCGGCAATTGTTAACACTTTCTTTAAAGCGATTGCGCTTCAc                                                                                                 <  2:1214663/148‑1 (MQ=255)

                                                  ttATCCATAGTAACGATGGCAGTTCAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTc                                                                                               <  1:648349/149‑1 (MQ=255)

                                                  ttATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGGGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGGGCTTCACTc                                                                                               >  2:1618594/1‑149 (MQ=255)

                                                  ttATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTc                                                                                               >  1:556798/1‑149 (MQ=255)

                                                  ttATCCAGAGTAACGATGGCAGTTAAAGCGACCATGCCATCCGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATCCCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTc                                                                                               <  1:364243/149‑1 (MQ=255)

                                                   tATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGCACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTcc                                                                                              <  1:1286231/149‑1 (MQ=255)

                                                    ttCCATAGTAACGATGGCATTTAAAGCGACCATGCCATTCGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCt                                                                                             <  2:555265/148‑1 (MQ=255)

                                                    aTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAACTCTGCTTGCATACCTGCGCCAGCCCTTTTGTCAGTACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTTACTCCt                                                                                             >  1:1746177/1‑149 (MQ=255)

                                                    aTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCCCTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTcca                                                                                             >  1:483986/1‑148 (MQ=255)

                                                    aTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGGACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGCGCTTCACTCCt                                                                                             >  1:56782/1‑149 (MQ=255)

                                                    aTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCt                                                                                             <  2:1946048/149‑1 (MQ=255)

                                                    aTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCt                                                                                             <  1:1537519/149‑1 (MQ=255)

                                                    aTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCt                                                                                             <  1:14552/149‑1 (MQ=255)

                                                    aTCCATAGAAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCt                                                                                             <  2:1980398/149‑1 (MQ=255)

                                                    aTCCAAAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCGTTAAAGCCATTGAGCTTCACTCCt                                                                                             <  1:1171835/149‑1 (MQ=255)

                                                     tCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTa                                                                                            <  2:1010943/149‑1 (MQ=255)

                                                     tCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTa                                                                                            <  1:1054054/149‑1 (MQ=255)

                                                     tCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTa                                                                                            <  1:1313871/149‑1 (MQ=255)

                                                     tCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTa                                                                                            <  1:722591/149‑1 (MQ=255)

                                                     tCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTa                                                                                            <  1:515857/149‑1 (MQ=255)

                                                     tCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAACTCTTGGAACGTTTTCAAATCTGCTTGCATCCCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTa                                                                                            <  1:1705866/149‑1 (MQ=255)

                                                     accATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCGTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTa                                                                                            <  2:264328/148‑1 (MQ=255)

                                                      ccATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTaa                                                                                           <  1:1784869/149‑2 (MQ=255)

                                                      ccATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTAc                                                                                           <  1:845699/149‑1 (MQ=255)

                                                       cATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCTTGAGCTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACa                                                                                          <  1:1975573/149‑1 (MQ=255)

                                                       cATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGTACCGGCAATTTTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACa                                                                                          <  2:694507/149‑1 (MQ=255)

                                                       cATAGTAACCATGCCAGTTAAAGCAACCAATCCACACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTGAAAACCTTCTTTAAAGCCACTGAGCTTCACTCCTACa                                                                                          <  2:902746/149‑1 (MQ=255)

                                                        aTAGTAAGGATTGCATTTAACGCGACCATGCCATACGTATCTAATTCTTTTAACGTTTTCAAATCGGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGTCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACat                                                                                         <  2:1519943/149‑1 (MQ=255)

                                                          aGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataa                                                                                       >  1:1511557/1‑149 (MQ=255)

                                                          aGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataa                                                                                       <  2:1849360/149‑1 (MQ=255)

                                                          aGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataa                                                                                       <  1:674318/149‑1 (MQ=255)

                                                          aGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATATGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataa                                                                                       >  1:278955/1‑149 (MQ=255)

                                                           gTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataat                                                                                      >  2:1654985/1‑149 (MQ=255)

                                                            tAACGATTGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataata                                                                                     <  1:935794/149‑1 (MQ=255)

                                                            tAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataata                                                                                     >  2:1840375/1‑149 (MQ=255)

                                                             aaCGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGTTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataa                                                                                    <  2:584303/149‑1 (MQ=255)

                                                               cGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATTTGGAATCTTACTTGAGCCCGCACGGGTTTCAGAACCGGCAATTGGTAAAACTGTCTTTAAAGCCATTGAGCTTCACTCCTACataataatt                                                                                  >  1:958446/1‑148 (MQ=255)

                                                                gagggCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGATCCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAatat                                                                                 <  1:1573530/146‑1 (MQ=255)

                                                                 aTGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATAtt                                                                                >  1:215339/1‑149 (MQ=255)

                                                                     cAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTTAAACTTCCTTTCAAGCCATTGAGCTTCACTCCTACATAATGATATTGtgt                                                                            >  1:865803/1‑147 (MQ=255)

                                                                     cAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCACTTGAGCTTCACTCCTACATAATAATATTGTAt                                                                            >  1:1663571/1‑149 (MQ=255)

                                                                          acaGCGACCATGCCAGACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATtcatc                                                                       <  1:849061/147‑1 (MQ=255)

                                                                              cGACCATGCCATTCGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcatat                                                                   >  2:1102919/1‑149 (MQ=255)

                                                                                       cATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTaa                                                          <  1:978199/149‑1 (MQ=255)

                                                                                           cGTATCTAATTCTTGTATCGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTa                                                      <  2:1810453/149‑1 (MQ=255)

                                                                                            gTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTaa                                                     >  1:953936/1‑149 (MQ=255)

                                                                                                 tAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGaa                                                >  1:297999/1‑149 (MQ=255)

                                                                                                    ttCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGaaaaa                                             >  2:24266/1‑149 (MQ=255)

                                                                                                      cTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAata                                           <  1:1502247/149‑1 (MQ=255)

                                                                                                        tGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATAtt                                         <  1:103747/149‑1 (MQ=255)

                                                                                                         ggAACGTTTTCAAATCTGCTTGCATCCCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATAATGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTa                                        <  1:705055/149‑1 (MQ=255)

                                                                                                          gAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTaa                                       >  2:1577854/1‑149 (MQ=255)

                                                                                                                 ttCAACTCTTCTTGCATCCCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCa                                <  2:407407/149‑1 (MQ=255)

                                                                                                                  tCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCaa                               <  2:44230/149‑1 (MQ=255)

                                                                                                                     aaTCTGTTGTCATACATGCGCAAGCAGTTTTGTAAGAACCGGCAATTGTTAAAAATTTCTTTAAAGCGATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAGCTAGAACATAATAAAAAAATATTAAGCATTCAatat                            <  2:428947/149‑1 (MQ=255)

                                                                                                                         tGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtga                        >  1:873625/1‑149 (MQ=255)

                                                                                                                         tGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtga                        >  1:931694/1‑149 (MQ=255)

                                                                                                                            ttGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtgatga                     <  2:1482020/149‑1 (MQ=255)

                                                                                                                                aTACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGAttgt                 <  1:917932/149‑1 (MQ=255)

                                                                                                                                 tACCTGCGCCAGCACTCTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGAttgtt                <  2:1262658/149‑1 (MQ=255)

                                                                                                                                     tGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGaaa            >  1:1435539/1‑149 (MQ=255)

                                                                                                                                       cgcCAGCACGTTTGTCAGAACCGGCAATTGTTCAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATGATTTTTAACCTAATTTAAAAATGTTAACAATACAATACTCGATAATGATTGatgta          >  2:845433/1‑145 (MQ=255)

                                                                                                                                          cAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAATGAAt       <  1:24266/149‑1 (MQ=255)

                                                                                                                                            gCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAATGAAtca     <  1:138437/149‑1 (MQ=255)

                                                                                                                                             cACTTTTGTCAGAACCTGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAATGAAtcat    <  1:426508/149‑1 (MQ=255)

                                                                                                                                               cTTTTGTCAGAACCGGCAAATGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAATGAAtcattc  <  2:230330/149‑1 (MQ=255)

TTTCAAATACATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAATGAATCATTC  >  USA300TCH1516_ALE/638468‑638759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`