Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,481,355 G→T 100% A6890E (GCA→GAA)  ebh_1 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,481,3550GT99.6% 142.2 / ‑6.9 51A6890E (GCA→GAA) ebh_1Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (27/22);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGC  >  USA300TCH1516_ALE/1481217‑1481491
                                                                                                                                          |                                                                                                                                        
cccGTCGCTCTATTGAGTTCCTTGGCGTTTTTAATTGCTTGCGTTACTTCAGCTACATTCTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTCTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCa                                                                                                                                        <  2:607296/141‑1 (MQ=255)
     cGCCGTATACAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGGGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATGGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGtt                                                                                                                                   >  2:474980/1‑141 (MQ=255)
              cAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATc                                                                                                                          <  1:421027/141‑1 (MQ=255)
                gTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTg                                                                                                                        >  2:714204/1‑141 (MQ=255)
                 ttacTGAGCGGTTTGATTGGCTTGCGTTACTTCAGCTACAGTTTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGt                                                                                                                       <  2:142685/138‑1 (MQ=255)
                 ttCCTGAGCGGTTTTGATGGCTTGCGTTGCTTCGGCTACAGGTGGCGCTTGATGGATTTTTCCAGTAACAGCACCTTTGTCTGCATTGTTTAAGTTATCTAACGTAGTTAATGTTTGGTTTTCATTGTTTTTAGCATCTGt                                                                                                                       <  2:101199/141‑1 (MQ=255)
                     tGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACt                                                                                                                   <  2:454674/141‑1 (MQ=255)
                      gAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACtt                                                                                                                  >  2:695997/1‑141 (MQ=255)
                         cGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTc                                                                                                               <  1:78861/141‑1 (MQ=255)
                                 aTGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCa                                                                                                       <  2:270262/141‑1 (MQ=255)
                                   ggCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCAtt                                                                                                     >  1:647260/1‑141 (MQ=255)
                                     cTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCa                                                                                                   >  1:15997/1‑141 (MQ=255)
                                     cTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCa                                                                                                   <  2:198893/141‑1 (MQ=255)
                                     cTTGCGTTACTTCAGCTACAGTGTGCGCATGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCa                                                                                                   >  1:499989/1‑141 (MQ=255)
                                         cGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTGTTTAGCATCTGTTACTTTTCTATCACCATTCAACGc                                                                                               >  1:194619/1‑141 (MQ=255)
                                          gTTACTTCAGCTACAGTGTGCGGTTGATTGATGTTTCCAGTAAGAGCACCTTTTTGTGCATTGTTTAAGTTATATAACGTAGATAATGTTTGGTTTTCATTGTTTTTGGCATCTGTTACTTTTCTATCACGATTCAACggt                                                                                              >  2:331926/1‑139 (MQ=255)
                                              cTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGtt                                                                                          >  1:118014/1‑141 (MQ=255)
                                                   gCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTa                                                                                     >  2:670649/1‑141 (MQ=255)
                                                    ttACAGTGTGCGCTTGATTCATGTTTCCAGTAACAGCTCCTTTTTTTGCATTGTTTAAGTTATCTAACGTATATAATTTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAg                                                                                    <  2:707946/140‑1 (MQ=255)
                                                      aCAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTACGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgtt                                                                                  <  2:295808/141‑1 (MQ=255)
                                                       cAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgttg                                                                                 >  2:291096/1‑141 (MQ=255)
                                                         gtgtGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgttgtt                                                                               >  2:566914/1‑141 (MQ=255)
                                                             gcgcTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATACTGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATAACCATTCAACGCTTGTTTCGTAGTTGTTACAg                                                                           >  1:88745/1‑141 (MQ=255)
                                                               gcTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTCCAGtt                                                                         >  1:337848/1‑141 (MQ=255)
                                                                    attgatGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTAGTTACAGTTGAAAg                                                                    >  2:192115/1‑141 (MQ=255)
                                                                            ttCCAGTAACAGCACCTTTTGGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCtt                                                            <  1:695997/141‑1 (MQ=255)
                                                                              ccAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCa                                                          >  1:755930/1‑141 (MQ=255)
                                                                                  tAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCCCCATTCAACGCTTGTTTCGTAGTTGTTACAGTTAGAAGTGCTGCTTCacttt                                                      >  2:457086/1‑137 (MQ=255)
                                                                                  tAACAGCACCTTTTTGGGCATTGTTTACGTTATCTAACGTAGATAAGGTATGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTCTCACCATTCAACGCTTGTTTCGTCGTTGTTTCAGTTGGAAGGGCTGCCTCAACtt                                                      >  2:74736/1‑141 (MQ=255)
                                                                                    aCAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTCCAGTTGAAAGTGCTGTCTCAACttct                                                    >  2:175434/1‑139 (MQ=255)
                                                                                    aCAGCACCTGTGTGTGCATTGTTTAAGTTATCTATCGTAGGTTATTTTTGGTTTTCACTGTTTTTAGCACCTGTTACGTTCCTCTCACCATTCATCGCTTGTTTCGGAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgt                                                    <  2:126547/141‑1 (MQ=255)
                                                                                       gCACCTTTGTGTTCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGAt                                                 <  1:669005/141‑1 (MQ=255)
                                                                                         aCCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTg                                               <  2:17436/141‑1 (MQ=255)
                                                                                          ccTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCCTTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTgg                                              <  1:601794/141‑1 (MQ=255)
                                                                                          ccTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACGGTTGAAAGTGCTGCTTCAACTTGTGATTgg                                              >  2:422581/1‑141 (MQ=255)
                                                                                          ccTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTgg                                              <  1:621003/141‑1 (MQ=255)
                                                                                               ttctgCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCGCCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGc                                         <  2:219020/138‑1 (MQ=255)
                                                                                                tgtgCATTGTTTAAGTTATCTAACGAAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCa                                        <  2:755003/141‑1 (MQ=255)
                                                                                                   gccttGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCAttt                                     <  2:433095/138‑1 (MQ=255)
                                                                                                     aTTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGt                                   <  2:437462/141‑1 (MQ=255)
                                                                                                          ttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTCCTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAt                              <  1:30456/141‑1 (MQ=255)
                                                                                                            aaGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAttt                            <  1:395456/141‑1 (MQ=255)
                                                                                                             aGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAGGTTCTGCTTCGACTTGTGATTGGTTCGCCTTTGTCCGATTTg                           >  2:143802/1‑141 (MQ=255)
                                                                                                             aGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTATTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTg                           >  2:502057/1‑141 (MQ=255)
                                                                                                              gTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGc                          >  2:139086/1‑141 (MQ=255)
                                                                                                                tATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCtt                        >  2:172256/1‑141 (MQ=255)
                                                                                                                 aTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTg                       >  2:535166/1‑141 (MQ=255)
                                                                                                                     aaCGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCGTTTGTACCATTTGCTTGATta                   >  1:311562/1‑141 (MQ=255)
                                                                                                                     aaCGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATta                   >  1:169920/1‑141 (MQ=255)
                                                                                                                       cGTAGATCATCTTCGTCTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACGATTTGCTTGATttat                 >  2:753404/1‑141 (MQ=255)
                                                                                                                             tAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCACCTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAGTAtt           >  1:27120/1‑141 (MQ=255)
                                                                                                                                 gtttggttttCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGTTTGATTAATAATATTTTca       >  2:67916/1‑141 (MQ=255)
                                                                                                                                 gtttggttttCATTGTGTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGATTGATTAATAATATTTTca       >  2:294291/1‑141 (MQ=255)
                                                                                                                                     tgttttCATTGTTTGTAGCATCTTTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTcagcag   <  1:285016/140‑1 (MQ=255)
                                                                                                                                      gttttCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTcagcagc  <  2:612323/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
CCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGC  >  USA300TCH1516_ALE/1481217‑1481491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑