Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,821,394 T→A 8.6% K122* (AAG→TAG)  flr_2 ← Flavoredoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,821,3940TA 8.6% 248.2 / 8.4 84K122* (AAG→TAG) flr_2Flavoredoxin
Reads supporting (aligned to +/- strand):  ref base T (38/38);  new base A (5/3);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAATACTGTCATCAATATGATAAGTTACAATTTCACCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTT  >  USA300TCH1516_ALE/2821258‑2821531
                                                                                                                                        |                                                                                                                                         
aaaaTACTGTCATCAATATGATAAGTTACAATTTCCCCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATc                                                                                                                                       <  2:253947/140‑1 (MQ=255)
    tACTGTCATCAATATGATAAGTTACAATTTCACCTATTATTAAATCAGCCCCATCTAATACCTCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTg                                                                                                                                   <  1:723711/141‑1 (MQ=255)
       tGTCATCAATATGATAAGTTACAATTTCACCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAg                                                                                                                                <  2:565987/141‑1 (MQ=255)
         tcatcaATATGATAAGTTACAATTTCACCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGAt                                                                                                                              <  1:265884/141‑1 (MQ=255)
            tcaATATGATAAGTTACAATTTCACCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGtt                                                                                                                           >  2:133044/1‑141 (MQ=255)
              aaTATGATAAGTTACAATTTCACCTATTATTAAATCAGACCCGTCTAATGCATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTaa                                                                                                                         >  1:339577/1‑141 (MQ=255)
                 atGATAAGTTACAATTTCACCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAg                                                                                                                      >  1:162039/1‑141 (MQ=255)
                   gATAAGTTACAATTTCACCTATTATTAAATCAGCCCCATCTAATACATATCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTg                                                                                                                    >  1:66166/1‑141 (MQ=255)
                        gTTACAATTTCACCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTAAATCGTTGTAGATGTTAAAAGTGATAAt                                                                                                               >  1:317789/1‑141 (MQ=255)
                        gTTACAATTTCACCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTAAATCGTTGTAGATGGTAAAAGTGATAAt                                                                                                               >  1:623625/1‑141 (MQ=255)
                           aCAATTTCACCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCt                                                                                                            <  1:325922/141‑1 (MQ=255)
                             cctttCACCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGt                                                                                                          <  1:138620/139‑1 (MQ=255)
                             aaTTTCACCTATTATTACATCAGCCCCCTCTAATACATCTTCAAGCAATACCATTTGCGATAGTTTACATTCGTATCGCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGt                                                                                                          <  2:733412/141‑1 (MQ=255)
                                ttCACCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACg                                                                                                       >  1:703513/1‑141 (MQ=255)
                                   aCCTATTATTAAACCAGCCCCCTCTAAGACGTCTCCTAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATGTTCGCATCTTTAATCCCTGGCGTCTTAAACGTTGTAGATGTTAAAAGTGATAATTCTGTACGact                                                                                                    <  1:23640/141‑1 (MQ=255)
                                     cTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGactca                                                                                                  >  2:643866/1‑141 (MQ=255)
                                      tattatTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGactcaa                                                                                                 >  1:703137/1‑141 (MQ=255)
                                      tattatTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCCGGCGTCTAAATCTCTGTAGATGTTAAAAGTGATAATTCTGTACGactcaa                                                                                                 <  2:127965/141‑1 (MQ=255)
                                       attattAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGactcaac                                                                                                <  1:89251/141‑1 (MQ=255)
                                         tattaAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTAAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGactcaactc                                                                                              <  1:409357/141‑1 (MQ=255)
                                          attaAATCAGCCCCATCTAATACATCTCCCAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTACTCACTATCGGCTTGCTCGTATTAGTGTTTAAAAGTGATAATTCTGTACGactcaactca                                                                                             <  1:371509/141‑1 (MQ=255)
                                           ttaGATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTTGCGTCTTAATCTTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCAc                                                                                            <  2:364689/141‑1 (MQ=255)
                                           ttaAATCAGCCCCATCTCAGCCATCTCCAAGCATTATCATTTGCGATAGTTTACATTCGAACCTCATTCTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTTATAATTCTGTACGACTCAACTCAc                                                                                            <  2:226084/141‑1 (MQ=255)
                                           ttaAATCAGCCCCATCTAATACCTCTCCCAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCAc                                                                                            <  2:326575/141‑1 (MQ=255)
                                           ttaAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCAc                                                                                            <  2:607577/141‑1 (MQ=255)
                                           ttaAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATGCTGTACGACTCAACTCAc                                                                                            <  2:243056/141‑1 (MQ=255)
                                             aaaTCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTg                                                                                          <  2:20230/141‑1 (MQ=255)
                                               aTCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTc                                                                                        >  1:704441/1‑141 (MQ=255)
                                               aTCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTc                                                                                        <  2:306197/141‑1 (MQ=255)
                                                 cAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGCTGTTAAAAGTGATAATTGTGTACGTCTCAACTTACTGTCAc                                                                                      >  2:75587/1‑141 (MQ=255)
                                                   gCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAGTCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACCCCCTGccacca                                                                                    >  1:62811/1‑141 (MQ=255)
                                                   gCCCCATCTAATACATCCCCAAGCAAGATCAATTGCGATAGTTTACATTCGAATCTCATATTCACATCTTTAATTCCTGGCGTCTAAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCa                                                                                    >  1:32819/1‑141 (MQ=255)
                                                    ccccATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCat                                                                                   >  1:710189/1‑141 (MQ=255)
                                                      ccATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATATCATTTTCGCATCTTTAATTCCTGGCGTCTTAATAGTTGTAGATGTTAAAAGTGATAGTTCTGTACGACTCAACTCACTGTAACCctat                                                                                 >  2:254439/1‑141 (MQ=255)
                                                        aTCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGc                                                                               <  2:255266/141‑1 (MQ=255)
                                                          cTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTa                                                                             >  2:190550/1‑141 (MQ=255)
                                                           tAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTCACCGTTGTAGATGTTAACGGTGCTAATTCTGTACGCCTAAACTCACAGTCACCATATGCTaa                                                                            >  2:106495/1‑141 (MQ=255)
                                                             aTACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCAGCTTTAATTCCTGGCGTCTTAATCGTTGTATATGTTAAAAGTGATAATTCTGTACGACTCAACTGACTGTAACCATATGCTAACg                                                                          >  1:278794/1‑141 (MQ=255)
                                                               aCATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACAATATGCTAAAggg                                                                        >  2:704508/1‑140 (MQ=255)
                                                                 aTCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGgcgc                                                                      <  2:506185/141‑1 (MQ=255)
                                                                  cccccAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAGAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCt                                                                     <  1:412395/138‑1 (MQ=255)
                                                                   ttcCACGCAATTTCATTTTCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTg                                                                    <  2:249016/140‑1 (MQ=255)
                                                                      cAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAg                                                                 >  1:228993/1‑141 (MQ=255)
                                                                      cAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCGAAATCGTTGTAGATGTTAAAAGTGATAATTCTGTGCGACCCAACTCAATGTCACCAACTGCTACCGGCGCTGCAg                                                                 >  2:238759/1‑141 (MQ=255)
                                                                        aGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGtc                                                               >  2:553415/1‑141 (MQ=255)
                                                                         gCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGtct                                                              >  1:701901/1‑141 (MQ=255)
                                                                          cAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGtctc                                                             <  1:55673/141‑1 (MQ=255)
                                                                             tatCATTTGCGATAGTTTACATTCGCATCTCTTTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGCCACCATATGCTCACGGCGCTGCAGTCTCAtt                                                          >  2:377218/1‑141 (MQ=255)
                                                                             tatCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCAtt                                                          >  1:649966/1‑141 (MQ=255)
                                                                             tatCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCAtt                                                          >  1:729812/1‑141 (MQ=255)
                                                                              atCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTAATTa                                                         >  2:249917/1‑141 (MQ=255)
                                                                                 aTTTGCGATCGTTTACATTCGAATCTCATTTTCGCATCTTTCGTTCCTGGCGTCTTATTGGTTGTAGATGTTAAAAGTGATACTTCTGTCCGGCTGAACTCACTTTTACTATATGTTATCGGCGCTGCAGTCTCATTattt                                                      >  2:617273/1‑138 (MQ=255)
                                                                                 aTTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAata                                                      >  1:47685/1‑141 (MQ=255)
                                                                                 aTTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAata                                                      <  1:726231/141‑1 (MQ=255)
                                                                                  tttGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAatat                                                     <  1:672401/141‑1 (MQ=255)
                                                                                      cGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTg                                                 <  1:441440/141‑1 (MQ=255)
                                                                                       gATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGa                                                <  1:704508/141‑1 (MQ=255)
                                                                                           gTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACAt                                            <  1:358874/141‑1 (MQ=255)
                                                                                               aCATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTACTCGAGGTGATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATc                                        <  1:564840/141‑1 (MQ=255)
                                                                                               aCATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATc                                        >  2:21262/1‑141 (MQ=255)
                                                                                               aCATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATc                                        <  1:166967/141‑1 (MQ=255)
                                                                                                cATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCCTTAATATCTTTAACATTATCt                                       >  2:101605/1‑141 (MQ=255)
                                                                                                cATTCGAATCTCATTTTCGCATCTTTAATTCCTGACGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCt                                       >  1:691314/1‑141 (MQ=255)
                                                                                                    cGAATCTCATTTTGGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGt                                   <  2:116023/141‑1 (MQ=255)
                                                                                                    cGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGt                                   >  1:177269/1‑141 (MQ=255)
                                                                                                     gAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTAACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAAAATTATCTTctgc                                  >  1:316633/1‑138 (MQ=255)
                                                                                                       aTCTCATTTTCGCATCTTTAATTCATGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTg                                >  1:566518/1‑141 (MQ=255)
                                                                                                        tctcATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGt                               <  1:774955/141‑1 (MQ=255)
                                                                                                          tcATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTaa                             >  2:612954/1‑141 (MQ=255)
                                                                                                              tttCGCATCTTTAATTCCTTGCGTCTTAATCTTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATg                         <  1:145439/141‑1 (MQ=255)
                                                                                                              tttCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATg                         <  2:710189/141‑1 (MQ=255)
                                                                                                               ttCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGc                        <  1:741752/141‑1 (MQ=255)
                                                                                                                   cATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCacaa                    >  2:351841/1‑141 (MQ=255)
                                                                                                                    aTCTTTAATTCCTGGCGTCTAAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCacaac                   >  2:539105/1‑141 (MQ=255)
                                                                                                                      cTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTACCTTCGTCTGCAATATGCAcaacaa                 >  1:423637/1‑141 (MQ=255)
                                                                                                                        ttAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGCCTGTAATATGCCCACCAAAg               >  1:99837/1‑141 (MQ=255)
                                                                                                                          aaTTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAACGtc             >  1:77257/1‑141 (MQ=255)
                                                                                                                          aaTTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGtc             <  2:701901/141‑1 (MQ=255)
                                                                                                                          aaTTCCTGGACTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACCACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAAGATCTTGAACATTATCTTCGTCTTTAATATGCACAACAAAGtc             <  2:117894/141‑1 (MQ=255)
                                                                                                                             tCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTcc          <  1:358687/141‑1 (MQ=255)
                                                                                                                              ccgggCGTATAAATCGTTGTTGATGTTAACAGTGAGAATACCGTCCAGCTCATGTCAGTTGCGCCATTTTCAATCGGGGCTGTAGTCTCAGTAATATTTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCa         <  2:66997/138‑1 (MQ=255)
                                                                                                                               cTGGCGTCTTAATCGTTGTAGATGTTAAAAGCGATAACTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGACCATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAg        <  1:106495/141‑1 (MQ=255)
                                                                                                                                   cGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATGTCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCg    >  2:594745/1‑141 (MQ=255)
                                                                                                                                     tCTTAATCGTTGTAGATGTTAAAAGTGATAAGTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGtt  >  2:165969/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TAAATACTGTCATCAATATGATAAGTTACAATTTCACCTATTATTAAATCAGCCCCATCTAATACATCTCCAAGCAATATCATTTGCGATAGTTTACATTCGAATCTCATTTTCGCATCTTTAATTCCTGGCGTCTTAATCGTTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTT  >  USA300TCH1516_ALE/2821258‑2821531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑