| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 547,929 | A→G | 11.6% | K191K (AAA→AAG) | ftsH → | ATP‑dependent zinc metalloprotease FtsH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 547,929 | 0 | A | G | 11.6% | 105.3 / 4.7 | 42 | K191K (AAA→AAG) | ftsH | ATP‑dependent zinc metalloprotease FtsH |
| Reads supporting (aligned to +/- strand): ref base A (20/17); new base G (2/3); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TAAATCTAAAGCAAAAATGTACGATAATAATAAACGTCGTGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGACTTTGTAGAGATGT > USA300TCH1516_ALE/547800‑548068 | aaaaTCTAAAGCAAAAATGTACGATAATAATAAACGTCGTGTTCGTCTCTCTGATGTAGCGGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATc < 2:106484/140‑1 (MQ=255) aaGCAAAAATGTACGATAATAATAGACGTCGTGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTc > 2:179122/1‑141 (MQ=255) aaGCAAAAATGTACGATAATAATAAACGTCGTGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTCATTTCTTGAAAGATAATAAAAAATTCGAAGAGATGGGATCTAGGATTc > 1:49017/1‑141 (MQ=255) gCAAAAATGTACGATAATAATAAACGTCGTGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCt < 1:134403/141‑1 (MQ=255) cAAAAATGTACGATAATAATAAACGTCGTGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTa > 1:23293/1‑141 (MQ=255) aaaTGTACGATAATAATAAACGTCGTGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGCTGATTTCATGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAg > 1:282240/1‑141 (MQ=255) aTGTACGATAATAATAAACGTCGTGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGgt > 1:317149/1‑141 (MQ=255) cGATAATAATAAACGGCGGGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCtt < 1:100245/141‑1 (MQ=255) ataataataAACGTCGTGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTat > 1:326344/1‑140 (MQ=255) taataaACGTCGTGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACttgt < 2:55915/141‑1 (MQ=255) aCGTCGTGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGGCTTACTTGTTGGAcc > 1:39868/1‑141 (MQ=255) gtTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTAGGACCTCCAggg > 2:55879/1‑140 (MQ=255) cGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAGGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACt > 1:316277/1‑141 (MQ=255) cGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAGGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACt > 1:143045/1‑141 (MQ=255) tctGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCAAGGTACTGGTaaa > 1:144692/1‑141 (MQ=255) tGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAAc < 2:62359/141‑1 (MQ=255) gCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACtt > 1:169961/1‑141 (MQ=255) gCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACtt > 1:158912/1‑141 (MQ=255) gCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTATTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACtt > 1:170053/1‑141 (MQ=255) ggggCAGATGCAGAAAAACAAGAATGAATTGAAATTGTTGATTGCGTGAAAGATAACAAAAAATGCAAAGAGATGGGATCTAGGATTCCTAAAGGTGGCTTACTTGTTGGACCTCCAGGTACTGGTAAAAAGTTACTTGCt > 2:90693/1‑141 (MQ=255) ggCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAGTGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTag < 1:10587/141‑1 (MQ=255) ggCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTag < 1:10585/141‑1 (MQ=255) aGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGc < 1:301898/141‑1 (MQ=255) aaaaCAAGAATTAATTGAAATTGTTACTGGTTTTGAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGATGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATGACTTGCTAGAGCGGTTGGAgg > 2:175489/1‑141 (MQ=255) aaCAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTg > 2:233847/1‑141 (MQ=255) gAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCa < 1:253596/141‑1 (MQ=255) gAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATGACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCa < 2:157486/141‑1 (MQ=255) gAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTgg > 1:383277/1‑141 (MQ=255) aaGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGtt > 2:369853/1‑141 (MQ=255) aataaAACATTTAAGGAAAGGGGATCTAGGAGTCCTAAAGTTGTCCTACTAGTGGCTCCTCCAGGTACTTTTAACATTTTGCTTGCTAGAGCGGTTGCAGGTGAAGCTCGCGCACCATTCTTCTCTATTAGTGGTTCATAc < 2:185787/141‑1 (MQ=255) aataaAAAATTCAAGGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGAc < 2:143045/141‑1 (MQ=255) aataaAAAATTCAAGGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGAc < 2:316277/141‑1 (MQ=255) ataaAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGACt < 2:169961/141‑1 (MQ=255) ataaAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGACt < 2:170053/141‑1 (MQ=255) ataaAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCACTCCATTCTTCTCTATTAGTGGTTCAGACt < 2:158912/141‑1 (MQ=255) taaAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCGGGTGACGCTGGCGCACCATTCTTCTCTATTGGTGGTTCAggagt > 2:111892/1‑137 (MQ=255) taaAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGACtt < 2:139241/141‑1 (MQ=255) taaAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGACtt < 2:139266/141‑1 (MQ=255) taaAAAATTCAAAGAAATGGGATCGAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGACtt < 2:49017/141‑1 (MQ=255) aaaaaaTTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTGTTCTCTATTAGTGGTTCAGACttt > 2:35277/1‑141 (MQ=255) aaaaaTTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGACTTTg < 1:20681/141‑1 (MQ=255) aaaaaTTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAAGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGACttgg > 2:96800/1‑139 (MQ=255) aaGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGACTTTGTAGAGAtgt > 1:67746/1‑141 (MQ=255) | TAAATCTAAAGCAAAAATGTACGATAATAATAAACGTCGTGTTCGTTTCTCTGATGTAGCAGGGGCAGATGAAGAAAAACAAGAATTAATTGAAATTGTTGATTTCTTGAAAGATAATAAAAAATTCAAAGAAATGGGATCTAGGATTCCTAAAGGTGTCTTACTTGTTGGACCTCCAGGTACTGGTAAAACATTACTTGCTAGAGCGGTTGCAGGTGAAGCTGGCGCACCATTCTTCTCTATTAGTGGTTCAGACTTTGTAGAGATGT > USA300TCH1516_ALE/547800‑548068 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |