BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	905,331	T→A	5.7%	I58I (ATT→ATA)	USA300HOU_RS04485 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	905,331	0	T	A	5.7%	401.8 / 5.9	131	I58I (ATT→ATA)	USA300HOU_RS04485	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (66/56); new base A (2/6); total (68/63)
Fisher's exact test for biased strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

CTATCTAACTTTGATGATATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTAAAGAAG  >  USA300TCH1516_ALE/905191‑905470

ataaccaaCTTTGAAGATATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAAtt                                                                                                                                             <  1:265395/135‑1 (MQ=255)

 tATCTAACTTTGATGATATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTa                                                                                                                                            <  2:120096/141‑1 (MQ=255)

 tATCTAACTTTGATGATATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTa                                                                                                                                            <  2:247037/141‑1 (MQ=255)

    cTAACTTTGATGATATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACa                                                                                                                                         <  2:160034/141‑1 (MQ=255)

           tgatgaTGTGAATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAatt                                                                                                                                  <  2:307895/141‑1 (MQ=255)

               gatATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAAttatta                                                                                                                              <  2:371656/141‑1 (MQ=255)

                atatGGATATTAAAGGAAAAGAAAAAATATATAAAAAGGATATAGGTAAAGATG‑TAAGTATGATCGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAAAAAAAAAAAAAAAAATTATTAg                                                                                                                             <  2:330530/141‑1 (MQ=255)

                 tatGGATATTAAAGGATATGCCGATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAgg                                                                                                                            >  2:279552/1‑141 (MQ=255)

                  atGGATATTAAAGGAAAAGAAAATATATAACAAAAGGATATAGGTAAAGATG‑TAAGTATGAGTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTAACAAAAAAAAAAAAAAAAAAATTATTAGGa                                                                                                                           <  2:175860/141‑1 (MQ=255)

                    ggATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAgt                                                                                                                         <  1:137537/141‑1 (MQ=255)

                      atatTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAgtgt                                                                                                                       <  1:35283/141‑1 (MQ=255)

                           aaaGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAat                                                                                                                  <  1:176933/141‑1 (MQ=255)

                           aaaGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAat                                                                                                                  <  2:209122/141‑1 (MQ=255)

                            aaGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAata                                                                                                                 >  2:35722/1‑141 (MQ=255)

                            aaGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGAGTTAAata                                                                                                                 >  2:100847/1‑141 (MQ=255)

                             aGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAAGAAAAAATTATTGGGAGTGTTAAatat                                                                                                                >  1:163251/1‑141 (MQ=255)

                             aGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAatat                                                                                                                >  1:362736/1‑141 (MQ=255)

                             aGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTAAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAatat                                                                                                                <  1:375776/141‑1 (MQ=255)

                                   tGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATatgaatga                                                                                                          <  1:254022/141‑1 (MQ=255)

                                       aaTCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTa                                                                                                      >  2:180657/1‑141 (MQ=255)

                                       aaTCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTa                                                                                                      >  1:237717/1‑141 (MQ=255)

                                        aTCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGGTTTATTACCAGAGCAAATTAACAAAAAATGATTAGGAGTGTTAAATATCAATGAAGTaa                                                                                                     >  2:310643/1‑141 (MQ=255)

                                           tataTCAGAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAGTTAACAAAAAATTATTAGGAGTGTTAAAACTGAATGAAGTAaatc                                                                                                  >  1:239088/1‑141 (MQ=255)

                                              atCAAAAGGATATAGGTAACGATGATTCATAT‑ATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAaatcaat                                                                                               >  1:185259/1‑141 (MQ=255)

                                              atCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAaatcaat                                                                                               >  1:254216/1‑141 (MQ=255)

                                              atCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATAATTAGGAGTGTTAAATATGAATGAAGTaaataaat                                                                                               >  2:85379/1‑141 (MQ=255)

                                              atCAAAAGAATATAGGTAAAGGTG‑TAAGTATGAGTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACAAGAAAAAAAAAAAAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAaatcaat                                                                                               <  2:205050/141‑1 (MQ=255)

                                                cAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTaaaaaaaaca                                                                                             >  2:284474/1‑134 (MQ=255)

                                                cAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAatcaatca                                                                                             >  1:206043/1‑141 (MQ=255)

                                                cAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAACAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTGAAATATGAATGAAATAAatcaatca                                                                                             >  2:79142/1‑141 (MQ=255)

                                                 aaaaGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTGTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAatcactcat                                                                                            >  2:284229/1‑140 (MQ=255)

                                                   aaGGATACAGGTAAAGATG‑TAAGCATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATCATTAGGGGTCTTAAATATGAATGAAGTAAACCAATCAAGt                                                                                          <  2:157640/141‑1 (MQ=255)

                                                     ggATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTCAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAACTTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTaa                                                                                        >  1:51261/1‑141 (MQ=255)

                                                      gATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAAt                                                                                       >  1:138841/1‑141 (MQ=255)

                                                          taGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACGGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGACTGAAGTAAATGAATCAAGTAATACAt                                                                                   >  1:231502/1‑141 (MQ=255)

                                                          taGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACAt                                                                                   >  2:362493/1‑141 (MQ=255)

                                                           aGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAATCAAATAAGTATTACTGAAACAGAAGTTTTATAACCAGAGCAAATTAACAAAAAATTTTTAGGAGTGTGAAATATGAATGAAGTAAATCAATCAAGTAAGACATg                                                                                  <  2:68492/141‑1 (MQ=255)

                                                           aGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATg                                                                                  >  2:78517/1‑141 (MQ=255)

                                                            ggTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATgg                                                                                 <  2:293022/141‑1 (MQ=255)

                                                             gTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAACAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATggg                                                                                <  2:81048/141‑1 (MQ=255)

                                                               aaaGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGggta                                                                              <  2:188715/141‑1 (MQ=255)

                                                                    tG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAACAATTATTAGAAGTGTTAAATCTGAATGCAGTACATCAATCAAGTAATACATGGGTAGGTAc                                                                         >  1:336249/1‑141 (MQ=255)

                                                                     g‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTAcc                                                                        >  1:116275/1‑141 (MQ=255)

                                                                     g‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTAcc                                                                        >  1:357684/1‑141 (MQ=255)

                                                                       tAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCt                                                                       <  1:30816/141‑1 (MQ=255)

                                                                       tAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCt                                                                       <  2:217863/141‑1 (MQ=255)

                                                                          gTATGATTGAACAGGGGCTAAAGCAACTAACGATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaa                                                                    >  2:159001/1‑141 (MQ=255)

                                                                            aTGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaaca                                                                  <  1:342788/141‑1 (MQ=255)

                                                                            aTGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaaca                                                                  >  1:14506/1‑141 (MQ=255)

                                                                             tGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaa                                                                 >  1:83017/1‑141 (MQ=255)

                                                                             tGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaa                                                                 >  1:315932/1‑141 (MQ=255)

                                                                              gATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaaa                                                                <  1:96010/141‑1 (MQ=255)

                                                                                ttGAACAGGGGTTAAATCAATGAAGTATTACTGAAACAGAAGTTTTATTACCAGCGCAAATTAACAAAAAATTATTCGGTGTGTTAAATATGAATGAAGTAAATCAATTCAGTAATACATGGGTACGCACCGTaacaaaac                                                              <  2:289425/141‑1 (MQ=255)

                                                                                ttGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaaaac                                                              <  2:133464/141‑1 (MQ=255)

                                                                                ttGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaaaac                                                              <  2:177176/141‑1 (MQ=255)

                                                                                ttGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaaaac                                                              <  1:125732/141‑1 (MQ=255)

                                                                                tggAACAGGTGTTAAAACAATCAAATATTAATGAAACAGAAGTTTTATTACCAAAGAAAAAAAAAAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaaaac                                                              <  2:184255/139‑1 (MQ=255)

                                                                                tggAAAAGGGGTTAAAGCAATTAAGTATTACTGAAACATAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaaaac                                                              <  2:108268/139‑1 (MQ=255)

                                                                                atGAACAGGTGTGAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATTAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaaaac                                                              <  1:60399/140‑1 (MQ=255)

                                                                                    aCAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAAtt                                                          >  1:110382/1‑141 (MQ=255)

                                                                                    aCAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAAtt                                                          >  1:60794/1‑141 (MQ=255)

                                                                                    aCAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAAAAAAACAAta                                                          >  2:171051/1‑140 (MQ=255)

                                                                                            tAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTtagta                                                  >  1:361380/1‑141 (MQ=255)

                                                                                            tAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTtagta                                                  <  2:264785/141‑1 (MQ=255)

                                                                                               aGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTg                                               <  1:291701/141‑1 (MQ=255)

                                                                                                gCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACCTGGGTAGGTACCTTAACAGAACAACTAGTTAGTAGTgg                                              >  2:301253/1‑140 (MQ=255)

                                                                                                   aTTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGaa                                           >  1:187259/1‑141 (MQ=255)

                                                                                                      aaGTATTACTGAAACAGAAGTTTTATTACGCGAAGCAGTAAAGAGAAAATTACTAAGGTATTTAAATATGACGGAAGTAAATCAATCAAGTAATACAGGGGGAGGTACCTTGAGCAGACAATTGGTTAGTAGTGAGAATaa                                        >  1:183700/1‑141 (MQ=255)

                                                                                                      aaGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATaa                                        >  1:246127/1‑141 (MQ=255)

                                                                                                       aGTATTACTGAAACAGAAGTTTTATTACCAGAGAAAATAAACAAAGAATTCTTGGGAGTGTTAACTATGAATGAAGTAAATCAATCAAGTAATAAATGCGTAGGTAACTTAACAAAACAATTCGTTAGTAGTGAGAATaaa                                       >  2:62701/1‑140 (MQ=255)

                                                                                                        gTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACt                                      <  1:226836/141‑1 (MQ=255)

                                                                                                            tACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTaa                                  <  2:63679/141‑1 (MQ=255)

                                                                                                               tGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAatat                               >  2:353390/1‑141 (MQ=255)

                                                                                                                gAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATAtt                              >  2:241329/1‑141 (MQ=255)

                                                                                                                gAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATAtt                              >  1:129766/1‑141 (MQ=255)

                                                                                                                gAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATAtt                              <  2:217053/141‑1 (MQ=255)

                                                                                                                gAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATAtt                              >  2:35144/1‑141 (MQ=255)

                                                                                                                  aaCAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAatatcaa                            >  2:93813/1‑138 (MQ=255)

                                                                                                                    cAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAag                          <  1:344228/141‑1 (MQ=255)

                                                                                                                    cAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAag                          >  1:60529/1‑141 (MQ=255)

                                                                                                                     aGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAaga                         >  2:292993/1‑141 (MQ=255)

                                                                                                                        aGTTTTATTACCAGAGCAAATTCACAAAAAATTACTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAACACACTTAGTTAGTCGTGAGAATAACTTTAATATTCAAGAGtt                      >  2:340272/1‑141 (MQ=255)

                                                                                                                        aGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCACGAGtt                      >  1:47983/1‑141 (MQ=255)

                                                                                                                         ttcttactaCCAGAGCAAATTAACAAAAAATTATTCGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTa                     <  1:79142/138‑1 (MQ=255)

                                                                                                                         gTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTa                     <  1:284474/141‑1 (MQ=255)

                                                                                                                         gTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTa                     <  2:206043/141‑1 (MQ=255)

                                                                                                                         gTTTTATGACCAAAGCAAATGAACAAAAAATTATTAGGAGGATTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTa                     <  1:366271/141‑1 (MQ=255)

                                                                                                                           tttATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTAcc                   <  2:369529/141‑1 (MQ=255)

                                                                                                                           tttATTACCAAAAAAAAAAAAAAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTAcc                   <  2:376396/141‑1 (MQ=255)

                                                                                                                               ttaCCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACt               <  2:361380/141‑1 (MQ=255)

                                                                                                                                tcccAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTg              <  1:346832/139‑1 (MQ=255)

                                                                                                                                taCCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTg              <  2:362736/141‑1 (MQ=255)

                                                                                                                                  ccAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGcc            >  2:339763/1‑140 (MQ=255)

                                                                                                                                  ccAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCa            >  1:261699/1‑141 (MQ=255)

                                                                                                                                    agagCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCCCGCACATCATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACg          <  1:182064/141‑1 (MQ=255)

                                                                                                                                        cAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAGGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTaaa      >  1:254764/1‑141 (MQ=255)

                                                                                                                                        cAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATAAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTaaa      >  1:72825/1‑141 (MQ=255)

                                                                                                                                         aaaTTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTACATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTAaag     >  2:204619/1‑141 (MQ=255)

                                                                                                                                          aaTTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTAaaga    >  2:90444/1‑141 (MQ=255)

                                                                                                                                           aTTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTAaagaa   <  1:99635/141‑1 (MQ=255)

CTATCTAACTTTGATGATATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTAAAGAAG  >  USA300TCH1516_ALE/905191‑905470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`