| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,532,298 | A→T | 100% | T15T (ACA→ACT) | femA_3 → | Aminoacyltransferase FemA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,532,298 | 0 | A | T | 100.0% | 86.8 / ‑6.5 | 30 | T15T (ACA→ACT) | femA_3 | Aminoacyltransferase FemA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (7/21); minor base G (0/1); total (7/23) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACG > USA300TCH1516_ALE/2532161‑2532435 | tgtgTATTTGTAATCAGATTTTGATAAAATAAAAAAATATCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGTGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAAAGCTTTTactaca > 2:181863/1‑141 (MQ=255) tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAAGAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt < 1:142012/141‑1 (MQ=255) aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTca < 2:240458/141‑1 (MQ=255) aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTca > 1:156427/1‑141 (MQ=255) aatttttGATAAAATAAAAAAATAGCATTATTAATAACTACATAAAGTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAt < 1:164548/139‑1 (MQ=255) ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac < 1:275535/141‑1 (MQ=255) ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACCTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac < 1:73945/141‑1 (MQ=255) gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacacac > 1:306830/1‑141 (MQ=255) aaataaaaAAAAAGCATCATTAATAAATTCAAAAATTATATCTCAATTCAGAGGGGGATGGGATAAAATGAACTTTGTAACTTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCATATTACACACAATc < 2:71479/141‑1 (MQ=255) taaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc < 1:148298/141‑1 (MQ=255) aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa < 1:55076/141‑1 (MQ=255) ataaATTCAAAAATTATAGTTCCATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAATCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATaga < 2:322545/141‑1 (MQ=255) ataaATTCAAAAATTATAGTTCAATTCAGAGAGCGATGGGATAAAATGAACTTCGGAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACATAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATaga < 2:210174/141‑1 (MQ=255) catagagGGCTGGGATAAACTGCACTTTTTAAAGTTGACTTCGGATAAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAACCCGCGATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt < 2:308078/138‑1 (MQ=255) cAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt < 1:343720/141‑1 (MQ=255) gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACCCAATCAGCTATTCATTACAATCATCGAGTTGATTTACAAGGCGATCTGAAtttcg > 2:245745/1‑137 (MQ=255) gggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg < 1:213997/141‑1 (MQ=255) ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAggg < 2:156427/141‑1 (MQ=255) taaCTTTGTAACGTTGACTACGGATGAGTTTAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa < 2:342048/140‑1 (MQ=255) aaCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAgg > 2:217972/1‑140 (MQ=255) aaCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGAAAATGGTCAAGt > 2:218016/1‑141 (MQ=255) gTTGACTTGGGTTGAGGTCAATGCGTTTACTACAAAGCATTTTTCACATGACACACAAACGGCTATGCATTACAATCAGAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt < 1:181863/141‑1 (MQ=255) gtcaCTTCGTATGAGTTCAATGCCTTTACTACAAAGCATCTTTCACATTACACACAATCAGCTATTCATTACAACCATAGAGTTGAGTTAAAAGGCGATGTGCATCTTGTAGGGGCTAAAGATGACAATGGTCAAGTGAtt < 1:46014/138‑1 (MQ=255) aCTTCGGATGAGTTCAATGCGTTTACGACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCa < 1:159334/141‑1 (MQ=255) ggATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGGTg > 2:4008/1‑141 (MQ=255) tCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGGGTTGATTTAAAAGGCGATGTGTATCTTGTAGTGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa < 2:306830/141‑1 (MQ=255) aaTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACa > 2:212883/1‑141 (MQ=255) aTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTTATTACAATCTTAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAg < 1:106298/141‑1 (MQ=255) ttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACACTCCTTAATTGGATTTAAACGGCGATGGGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcac < 1:72395/141‑1 (MQ=255) ttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcac < 1:289356/141‑1 (MQ=255) tACCACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGCTGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcacg < 1:168270/141‑1 (MQ=255) | TGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACG > USA300TCH1516_ALE/2532161‑2532435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |