Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 1,232,465 G→C A124P (GCG→CCG)  prkC → Serine/threonine‑protein kinase PrkC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,232,4650GC100.0% 346.0 / NA 98A124P (GCG→CCG) prkCSerine/threonine‑protein kinase PrkC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (58/40);  total (58/40)

AAGAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCATGT  >  USA300TCH1516_ALE/1232328‑1232601
                                                                                                                                         |                                                                                                                                        
aagaagATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATccgc                                                                                                                                       >  2:98074/1‑141 (MQ=255)
aagaagATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATccgc                                                                                                                                       <  1:975895/141‑1 (MQ=255)
 agaagaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCa                                                                                                                                      <  1:1090270/141‑1 (MQ=255)
 agaagaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCa                                                                                                                                      <  1:1091021/141‑1 (MQ=255)
 agaagaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCa                                                                                                                                      <  1:1091099/141‑1 (MQ=255)
 agaagaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCa                                                                                                                                      <  1:363696/141‑1 (MQ=255)
   aagaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAATGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATg                                                                                                                                    <  2:66215/141‑1 (MQ=255)
     gaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGat                                                                                                                                  >  2:131156/1‑141 (MQ=255)
     gaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGat                                                                                                                                  <  2:1183419/141‑1 (MQ=255)
        gACTGTTACTACTTAGTAATGGAATATTTCTAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATg                                                                                                                               <  2:784553/141‑1 (MQ=255)
         aCTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGc                                                                                                                              <  2:618006/141‑1 (MQ=255)
             ttACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCACATATTGGATGGCATTAAACATCCGCATGATATGCGTAt                                                                                                                          >  2:656184/1‑141 (MQ=255)
             ttACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGCTGGCATTAAACATCCGAATGATATGCGTAt                                                                                                                          >  2:111772/1‑141 (MQ=255)
             ttACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAAATTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTAt                                                                                                                          >  2:655997/1‑141 (MQ=255)
               actactTAGTAATTGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGCAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTg                                                                                                                        <  2:397423/141‑1 (MQ=255)
                   ctTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGACATGCGTATTGTACa                                                                                                                    <  1:1036455/141‑1 (MQ=255)
                          aTGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGat                                                                                                             >  1:460230/1‑141 (MQ=255)
                                tatatCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAg                                                                                                       >  1:65951/1‑141 (MQ=255)
                                   atCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCca                                                                                                    >  2:47520/1‑141 (MQ=255)
                                    tCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTTGCACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCgac                                                                                                   >  2:492037/1‑141 (MQ=255)
                                    tCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCcac                                                                                                   <  1:326352/141‑1 (MQ=255)
                                    tCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCcac                                                                                                   <  2:404050/141‑1 (MQ=255)
                                     cGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCcaca                                                                                                  >  2:151970/1‑141 (MQ=255)
                                     cGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCcaca                                                                                                  >  1:755004/1‑141 (MQ=255)
                                           tCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatat                                                                                            >  1:720623/1‑141 (MQ=255)
                                            ccGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGGTAGCAATAAACATCCGCATGATATGCGTATTGGACATAGAGATATTAAGCCACAAAatgta                                                                                           >  1:571275/1‑141 (MQ=255)
                                            ccGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCCCAAAatata                                                                                           >  2:308270/1‑141 (MQ=255)
                                            ccGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatata                                                                                           >  1:978198/1‑141 (MQ=255)
                                            ccGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatata                                                                                           >  2:87377/1‑141 (MQ=255)
                                            ccGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatata                                                                                           <  2:1195123/141‑1 (MQ=255)
                                            ccGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAataaa                                                                                           >  2:632198/1‑139 (MQ=255)
                                            ccGACTTTGTCTGACTATAGTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatata                                                                                           >  1:661865/1‑141 (MQ=255)
                                             caaCTTTGTCGGAGTAAATTGAAAATCAAGAGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatatat                                                                                          <  1:481711/139‑1 (MQ=255)
                                             cGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATGGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatatat                                                                                          <  2:725974/141‑1 (MQ=255)
                                             cGACGTTGTCTGAGTAGATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGCTTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAACCCACAAAatatat                                                                                          >  2:170167/1‑141 (MQ=255)
                                              gACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAAGCACAAAATATAtt                                                                                         >  2:948371/1‑141 (MQ=255)
                                               aCTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCCCAAAATATATTa                                                                                        >  1:116216/1‑141 (MQ=255)
                                               aCTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAAAATATTa                                                                                        >  1:736089/1‑141 (MQ=255)
                                                       tGAGTATATTGAAAGTCATGGACCATTAAGTGTTGACACAGAGATTAAGTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATAAGCATCTAGTACATAGAGATATTAAGCGACAAAAGATATTGATTAACAg                                                                                >  1:624277/1‑141 (MQ=255)
                                                          gTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCaa                                                                             >  1:382145/1‑141 (MQ=255)
                                                               ttGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATaaaa                                                                        >  2:740855/1‑141 (MQ=255)
                                                                 gAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACg                                                                      <  2:1130882/141‑1 (MQ=255)
                                                                  aaaGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGt                                                                     >  2:966294/1‑141 (MQ=255)
                                                                  aaaGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGt                                                                     >  2:1014944/1‑141 (MQ=255)
                                                                   aaGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGtt                                                                    >  1:748902/1‑141 (MQ=255)
                                                                   aaGTCAAGGGCCATAAAATGTAGACAAAGCGATTAATTATACAAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGtt                                                                    <  1:967756/141‑1 (MQ=255)
                                                                    aggcATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTg                                                                   <  2:500822/138‑1 (MQ=255)
                                                                        aTGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaaaa                                                               >  2:811643/1‑141 (MQ=255)
                                                                          gggCCATTAATGGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGttttaatgt                                                             <  2:945723/141‑8 (MQ=255)
                                                                          gggCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATCTTGGATGACATGAAACATCCGCAGGATATGCGTATCGTACATAGAGATATTAAGCCACAAAATATATTAATCGACCGCAATAAAACGTTGagaaaa                                                             >  2:135300/1‑139 (MQ=255)
                                                                          gggCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAata                                                             <  1:98074/141‑1 (MQ=255)
                                                                             ccATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACGCCAATAAAAAGTTAAAAAAAttt                                                          >  2:506187/1‑141 (MQ=255)
                                                                              cATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTg                                                         >  1:68613/1‑141 (MQ=255)
                                                                                ttAAGTGATAACACAGCGATTAATTTTACGAATCAAATATTGGAGGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGAAATTAAGCCAGAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAt                                                       <  2:640385/141‑1 (MQ=255)
                                                                                atAAGTGTTGACACAGCGAATAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGACATTAAGCCACAAAATATATTAATTGCCAGCAATAAAACGTTGAAAATATTTGAt                                                       <  1:778811/140‑1 (MQ=255)
                                                                                 tAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATGTTTGAtt                                                      >  1:590586/1‑141 (MQ=255)
                                                                                 tAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGAGATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAtt                                                      >  1:691182/1‑141 (MQ=255)
                                                                                  aaGTTTTGAAACAGCGATTAATTTTACGAATCAAATATTGGAGGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAttt                                                     <  1:929035/141‑1 (MQ=255)
                                                                                  aaGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGTGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAttt                                                     >  2:97573/1‑141 (MQ=255)
                                                                                   aGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACGTAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAtttt                                                    >  2:873979/1‑141 (MQ=255)
                                                                                   aGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAAAATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAACATTTGAtgtt                                                    >  2:566497/1‑141 (MQ=255)
                                                                                     tgtTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTgg                                                  >  2:363822/1‑141 (MQ=255)
                                                                                       ttGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGaa                                                >  1:938790/1‑141 (MQ=255)
                                                                                         tacacAGCGATTAATTTGACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAACATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAAtt                                              <  2:1191617/140‑1 (MQ=255)
                                                                                         gACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATAGGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGGAAATATTTGTATTTGGAAtt                                              >  2:220663/1‑141 (MQ=255)
                                                                                          acacAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTg                                             <  1:1149025/141‑1 (MQ=255)
                                                                                           cacaGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAAttac                                            >  1:43870/1‑139 (MQ=255)
                                                                                           cacaGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGc                                            >  2:976261/1‑141 (MQ=255)
                                                                                           cacaGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATACTAAGCCACAAAATATATTAATTGTCAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGc                                            >  1:815540/1‑141 (MQ=255)
                                                                                           aacaGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGc                                            <  1:1028700/140‑1 (MQ=255)
                                                                                                 gATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGc                                      <  2:718359/141‑1 (MQ=255)
                                                                                                 gATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGc                                      <  1:1145153/141‑1 (MQ=255)
                                                                                                  aTTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCt                                     <  1:1171880/141‑1 (MQ=255)
                                                                                                  aTTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCt                                     >  1:1203960/1‑141 (MQ=255)
                                                                                                   ttAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAACtt                                    >  2:261035/1‑141 (MQ=255)
                                                                                                    tAATTTTACGAATCAAAAATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCttt                                   <  1:742052/141‑1 (MQ=255)
                                                                                                    tAAGTTTAAAAATCAAAAATGGTAAGACATAAAACAACCGCAAAATATACGAAGTGTACATAGAGATATTAAGCCACAAAATAAATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGTAATTGCTAAAGCttt                                   <  1:157913/141‑1 (MQ=255)
                                                                                                            cGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTgaga                           >  1:1096825/1‑141 (MQ=255)
                                                                                                            cGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTgaga                           >  1:1168846/1‑141 (MQ=255)
                                                                                                               aTCAAATATTGGATGGCATTAAACCTCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGGGAGGt                        >  2:1164403/1‑141 (MQ=255)
                                                                                                                tCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTc                       <  2:661865/141‑1 (MQ=255)
                                                                                                                 cAAGTATTGGAGTGCATTAAACATCCGCATGATATGGGTATTGTACATAGAGATAATAAGACACAAAATATATTAATTGACAGCAATAAAACGTTCAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCt                      <  2:946389/141‑1 (MQ=255)
                                                                                                                  aaaTATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                     >  2:717472/1‑141 (MQ=255)
                                                                                                                  aaaTATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                     <  2:371445/141‑1 (MQ=255)
                                                                                                                  aaaTATTGGATGGCATTAAACATCCGCATGATATGCGTATTGAAAATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                     >  2:851011/1‑141 (MQ=255)
                                                                                                                  aaaTATTGGATGGCATTAAACATCCGCATGATATGAGTATTGTACATAGAGATGTTAAGCCACAGAATAGATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                     >  2:301651/1‑141 (MQ=255)
                                                                                                                   aaTATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGAGGTCttt                    >  2:603688/1‑141 (MQ=255)
                                                                                                                    atatTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTa                   <  1:205970/141‑1 (MQ=255)
                                                                                                                      atTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAAc                 >  1:88353/1‑141 (MQ=255)
                                                                                                                         ggATGGCATTAAACATCCGCATGAGATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTGGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCa              >  2:308677/1‑141 (MQ=255)
                                                                                                                          gATGGCATTAAACAGCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAg             <  2:666343/141‑1 (MQ=255)
                                                                                                                           aTGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGa            <  2:382145/141‑1 (MQ=255)
                                                                                                                           aTGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGa            >  1:957924/1‑141 (MQ=255)
                                                                                                                            tGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATGAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGAc           <  2:603502/141‑1 (MQ=255)
                                                                                                                              gCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTa         >  1:338775/1‑141 (MQ=255)
                                                                                                                                 ttAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAAta      >  2:328136/1‑140 (MQ=255)
                                                                                                                                 ttAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATc      >  1:519431/1‑141 (MQ=255)
                                                                                                                                    aaCATCCGCATGATATGCGTATTGTACTATGATATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATAAtg   >  1:581272/1‑141 (MQ=255)
                                                                                                                                    aaCATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCAAAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAtg   <  1:131156/141‑1 (MQ=255)
                                                                                                                                     aCATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAtgt  <  1:351361/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
AAGAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCATGT  >  USA300TCH1516_ALE/1232328‑1232601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑