BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,474,639	C→G	A9129P (GCA→CCA)	ebh_1 ←	Extracellular matrix‑binding protein ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,474,639	0	C	G	100.0%	386.8 / NA	108	A9129P (GCA→CCA)	ebh_1	Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (55/53); total (55/53)

Only 100 of 108 total aligned reads displayed.

CGACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGCTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTCATTA  >  USA300TCH1516_ALE/1474503‑1474775

 gACCTTTAAATCCATCTTGTTGAGCATTGTTTAGTGCATTCAATTGATTGTTGTCATGTTTACAATGTGTTTTACCAAGTGCTTCACGATCTGTCCCATTTAATGCTTGTTTTGAATTTACAATATTTTGCAGTGGTTgtt                                                                                                                                     >  2:327913/1‑139 (MQ=255)

 gACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGAt                                                                                                                                     <  1:843371/141‑1 (MQ=255)

 gACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGAt                                                                                                                                     >  2:5216/1‑141 (MQ=255)

 gACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGATCGATGGCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCCTGTTTTGCCTTTAAAATATTTTGAAGTGGTTgct                                                                                                                                     >  1:632560/1‑139 (MQ=255)

 gACCTTTAAATCCATCATGTTGAGCAATGTATAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTGGACGGGGTTGAt                                                                                                                                     >  2:477162/1‑141 (MQ=255)

   ctttttAATCCATCTTGTTGAGCAATGTTTACTGCATTCAATTGGTCGATGTCATGTATATCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATcc                                                                                                                                   <  1:874747/139‑1 (MQ=255)

   ccTTTAAATCCATCTTGTTGAGCATTGTTTTATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATcc                                                                                                                                   <  2:662867/141‑1 (MQ=255)

   ccTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATcc                                                                                                                                   <  1:1039271/141‑1 (MQ=255)

   ccTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATcc                                                                                                                                   <  1:957235/141‑1 (MQ=255)

    cTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATTTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCa                                                                                                                                  >  1:231536/1‑141 (MQ=255)

    cTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCa                                                                                                                                  <  1:650126/141‑1 (MQ=255)

     ttcaaaTCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCAc                                                                                                                                 <  1:246082/138‑1 (MQ=255)

       tAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACtt                                                                                                                               >  2:1121210/1‑141 (MQ=255)

           tCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACttgttg                                                                                                                           >  1:1109028/1‑141 (MQ=255)

            ccATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGc                                                                                                                          <  2:1130369/141‑1 (MQ=255)

             cATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCt                                                                                                                         <  1:1129295/141‑1 (MQ=255)

              ctCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCtt                                                                                                                        <  2:326883/140‑1 (MQ=255)

                  tgttgAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTc                                                                                                                    >  1:1191388/1‑141 (MQ=255)

                  tgttgAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTc                                                                                                                    >  1:645631/1‑141 (MQ=255)

                    ttgAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGc                                                                                                                  <  1:735459/141‑1 (MQ=255)

                    ttgAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGc                                                                                                                  <  2:78601/141‑1 (MQ=255)

                     tgAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCa                                                                                                                 <  1:983374/141‑1 (MQ=255)

                            tGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTACTGCTAGCTGTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGtt                                                                                                          >  1:1187123/1‑141 (MQ=255)

                            tGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGtt                                                                                                          >  1:839800/1‑141 (MQ=255)

                            tGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGtt                                                                                                          >  1:108924/1‑141 (MQ=255)

                                tAATGCATTCAATTGGTCGATGTCATCTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATCTTTTGAAGTGGTTGATCCGGTTGTTGCTTATTCGCATTTTGTTTCat                                                                                                      >  2:746523/1‑140 (MQ=255)

                                   tGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATAc                                                                                                   <  2:811871/141‑1 (MQ=255)

                                    gCATTCAATTGGTCGATGTCATGATTACCATTTGTTTTAGCAAGTGCTACACGTCCATCACCATTTAATGCTTGCTTTGCATTTAAAATACTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACa                                                                                                  >  1:108731/1‑141 (MQ=255)

                                      aTTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACAtt                                                                                                <  1:1009692/141‑1 (MQ=255)

                                      aTTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACAtt                                                                                                <  2:473982/141‑1 (MQ=255)

                                        tCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACAtttt                                                                                              <  1:177179/141‑1 (MQ=255)

                                        tCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACAtttt                                                                                              <  2:1114571/141‑1 (MQ=255)

                                         cAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtc                                                                                             <  1:263024/141‑1 (MQ=255)

                                           aTTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcag                                                                                           <  2:613969/141‑1 (MQ=255)

                                           aTTGGTCGATGTCAGGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCGTTTTGTTTCAATAATTTTtcag                                                                                           >  1:209057/1‑141 (MQ=255)

                                            ttGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTTGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagc                                                                                          >  2:176215/1‑141 (MQ=255)

                                              ggTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTCATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCTTTTTGTTTCAATACATTTtcagcat                                                                                        >  1:1003668/1‑141 (MQ=255)

                                              ggTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagcat                                                                                        >  1:211461/1‑141 (MQ=255)

                                               gTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagcatc                                                                                       >  2:350627/1‑141 (MQ=255)

                                               gTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagcatc                                                                                       >  1:395726/1‑141 (MQ=255)

                                                tctaTGTCATTTCTACCATTTGTTTTAGCTAGTACTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTGGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTCCAATACATTTtcagcatca                                                                                      <  2:672442/138‑1 (MQ=255)

                                                      tCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATa                                                                                <  1:413511/141‑1 (MQ=255)

                                                      tCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATa                                                                                >  1:1145344/1‑141 (MQ=255)

                                                       catttctACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAg                                                                               <  2:908936/135‑1 (MQ=255)

                                                       cATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTGAGCTTCAGCAATAg                                                                               <  2:816297/141‑1 (MQ=255)

                                                       cATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAg                                                                               >  2:461583/1‑141 (MQ=255)

                                                        aTGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGc                                                                              >  2:1186019/1‑141 (MQ=255)

                                                         tGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCt                                                                             >  2:504681/1‑141 (MQ=255)

                                                         ggTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAACATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCt                                                                             <  1:50592/140‑1 (MQ=255)

                                                          gTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGATTATTCGCGTTTTGTTTCAATACATTTTCGGCATCAGCAATAGCtt                                                                            >  1:328991/1‑141 (MQ=255)

                                                                  ttttttttAGCAAGTGCTACACGTTCATTACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTATTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATaa                                                                    <  2:782749/141‑1 (MQ=255)

                                                                  tttGTTTTTGCAAGTTCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATaa                                                                    <  2:216062/141‑1 (MQ=255)

                                                                  tttGTTTTAGCAAGTGGTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATaa                                                                    <  1:856328/141‑1 (MQ=255)

                                                                  tttGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATaa                                                                    <  1:978375/141‑1 (MQ=255)

                                                                   ttGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAg                                                                   <  2:1111178/141‑1 (MQ=255)

                                                                   ttGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAg                                                                   <  1:1121210/141‑1 (MQ=255)

                                                                   ttGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAg                                                                   <  1:174978/141‑1 (MQ=255)

                                                                    tGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGc                                                                  >  1:649516/1‑141 (MQ=255)

                                                                    tGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGc                                                                  <  1:1095072/141‑1 (MQ=255)

                                                                     gTTTTAGCAAGTGCTAAACGTACATCACACTTTAATGCTTGTTTTGCATGAAAGAAAGCTTGAAGTGGTTGATCCACGCGCTGCTTGTACGCATTTTGTTACAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCa                                                                 >  2:344905/1‑141 (MQ=255)

                                                                      ttttAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCAt                                                                <  1:674740/141‑1 (MQ=255)

                                                                          aGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAGAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTg                                                            >  2:780638/1‑141 (MQ=255)

                                                                          aGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTg                                                            >  1:688219/1‑141 (MQ=255)

                                                                           gCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGt                                                           >  1:1106051/1‑141 (MQ=255)

                                                                            cAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGtt                                                          >  1:1070367/1‑141 (MQ=255)

                                                                              aGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTa                                                        <  2:1191388/141‑1 (MQ=255)

                                                                              aGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTa                                                        <  2:645631/141‑1 (MQ=255)

                                                                                tGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATc                                                      <  1:4244/141‑1 (MQ=255)

                                                                                  cTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTGGCATTTTGTTTCAGTACATTTTCAGCATCAGCAATAGCTGGTTTATAAGCATTTTGTTTATCTg                                                    >  1:1156802/1‑141 (MQ=255)

                                                                                    acacGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGaa                                                  >  1:997499/1‑141 (MQ=255)

                                                                                    acacGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGaa                                                  <  1:726899/141‑1 (MQ=255)

                                                                                       ctttCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGTATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTCTGTTTATCTGAATcc                                               <  2:639160/139‑2 (MQ=255)

                                                                                       cGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCt                                               <  2:1178073/141‑1 (MQ=255)

                                                                                        gTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTg                                              <  1:637633/141‑1 (MQ=255)

                                                                                          tcatcaCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCt                                            <  1:1208955/141‑1 (MQ=255)

                                                                                           catcaCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCtt                                           >  1:418618/1‑141 (MQ=255)

                                                                                            tttaCCATTTAATTCTTGTTTTTTATTTAAAATATTTTGACGTGGTTTATCCATTTGTTGCTTATTGGCATTTTCTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtg                                          <  2:409531/138‑1 (MQ=255)

                                                                                            atcaCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtg                                          >  1:997537/1‑141 (MQ=255)

                                                                                             tcaCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtgt                                         <  2:854180/141‑1 (MQ=255)

                                                                                              caCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTCTAAGCATTTTGTTTTTCTTAATCTGCTtgtg                                        >  2:780347/1‑141 (MQ=255)

                                                                                              caCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtgtg                                        <  1:504681/141‑1 (MQ=255)

                                                                                              caCCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtgtg                                        >  2:1172733/1‑141 (MQ=255)

                                                                                                ccATTTAATGCTGGTTTTGCATTTAAAATATGTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtgtgtg                                      <  1:477162/141‑1 (MQ=255)

                                                                                                   tttAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCCATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTaa                                   <  1:941134/141‑1 (MQ=255)

                                                                                                   tttAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATAAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTaa                                   <  1:941049/141‑1 (MQ=255)

                                                                                                   tttAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTAAGCTGCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTaa                                   <  1:522243/141‑1 (MQ=255)

                                                                                                     tAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGATTGAGGTTACTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATc                                 <  1:1186019/141‑1 (MQ=255)

                                                                                                      aaTGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCt                                >  2:171305/1‑141 (MQ=255)

                                                                                                       aTGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTa                               >  2:1208601/1‑141 (MQ=255)

                                                                                                       aTGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGGGTGTAATCTa                               >  2:410876/1‑141 (MQ=255)

                                                                                                             tttttGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATGAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGttt                         <  2:642355/140‑1 (MQ=255)

                                                                                                              ttttGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTGGTGTGTAATCTACAGTTTg                        >  1:356940/1‑141 (MQ=255)

                                                                                                                      ttAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTAACCGt                >  2:17196/1‑141 (MQ=255)

                                                                                                                       tAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGtt               >  1:107747/1‑141 (MQ=255)

                                                                                                                       tAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGtt               >  1:499397/1‑141 (MQ=255)

                                                                                                                       tAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGtt               >  1:985391/1‑141 (MQ=255)

                                                                                                                         aaaTATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAg             <  2:163783/141‑1 (MQ=255)

                                                                                                                          aaTATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTGCAGTTTGCTCAACGTTAGc            >  1:523583/1‑141 (MQ=255)

                                                                                                                          aaTATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGc            >  2:586992/1‑141 (MQ=255)

                                                                                                                                 tGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTAAGCAGCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGTTTGTGTGTAATCTACAGTTTGTTGAACGTTAGCATGTTCa     >  1:522605/1‑141 (MQ=255)

CGACCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGCTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTCATTA  >  USA300TCH1516_ALE/1474503‑1474775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`