BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,481,355	G→T	A6890E (GCA→GAA)	ebh_1 ←	Extracellular matrix‑binding protein ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,481,355	0	G	T	100.0%	307.7 / NA	100	A6890E (GCA→GAA)	ebh_1	Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (51/49); total (51/49)

Only 100 of 104 total aligned reads displayed.

CCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGC  >  USA300TCH1516_ALE/1481217‑1481491

cccATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCa                                                                                                                                        >  1:609892/1‑141 (MQ=255)

 ccATCGCTGTATTCAGTTCGTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCTGTAACAGCATCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCAt                                                                                                                                       <  2:286883/141‑1 (MQ=255)

     cGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGtt                                                                                                                                   <  1:612093/141‑1 (MQ=255)

      gCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTGCAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGttt                                                                                                                                  >  1:232199/1‑141 (MQ=255)

      gCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTCCAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGttt                                                                                                                                  >  2:106439/1‑141 (MQ=255)

      gCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCAtttttt                                                                                                                                  >  2:694780/1‑141 (MQ=255)

      gCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGttt                                                                                                                                  <  1:1000655/141‑1 (MQ=255)

         gTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTa                                                                                                                               >  2:436849/1‑141 (MQ=255)

           aTTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGc                                                                                                                             <  1:193886/141‑1 (MQ=255)

                gTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCGTCtt                                                                                                                        >  1:175678/1‑140 (MQ=255)

                gTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTg                                                                                                                        >  1:1073289/1‑141 (MQ=255)

                     tGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACt                                                                                                                   <  1:518210/141‑1 (MQ=255)

                     tGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACt                                                                                                                   <  1:114133/141‑1 (MQ=255)

                       aGCGATTTGAATGGCGTGCGTTAATTAAGCTACAGTGTGCGCTCGATAGAGGTTTCCAGTGACAGCACGTTTTTGTGCATTGTGTAAGTTATCTAACGTAGATGATGTTTGGTCTTCATTGTTTTTAGCAGCTTCTACttt                                                                                                                 >  2:168141/1‑141 (MQ=255)

                        gCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATACTGTTAGGTATTAATTGTTTATAACATCTGTTACttgt                                                                                                                >  2:1137107/1‑139 (MQ=255)

                        gCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACtttt                                                                                                                >  1:1110263/1‑141 (MQ=255)

                         cGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTc                                                                                                               <  1:427516/141‑1 (MQ=255)

                         cGGTTTAAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACttttt                                                                                                               >  2:667127/1‑140 (MQ=255)

                          ggTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCt                                                                                                              <  1:1011125/141‑1 (MQ=255)

                                aaTGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCAcc                                                                                                        <  1:835010/141‑1 (MQ=255)

                                  tGGCTTGCGTTACTTCAGCGACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCAt                                                                                                      <  2:677836/141‑1 (MQ=255)

                                       tGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAAc                                                                                                 >  2:132794/1‑141 (MQ=255)

                                        gCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACg                                                                                                <  2:394260/141‑1 (MQ=255)

                                        gCGTTACTTCAGCTACAGTGAGCGTGTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACg                                                                                                <  1:215400/141‑1 (MQ=255)

                                         cGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTCAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGc                                                                                               >  2:136608/1‑141 (MQ=255)

                                            tACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTCTCACCATTCCACGCTTg                                                                                            >  2:465949/1‑141 (MQ=255)

                                              tttCAGCTCCTGTGTGCGTTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGtt                                                                                          <  2:1136090/140‑1 (MQ=255)

                                              cTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCCCCTTTTTGTGCATTGTTTAAGTTACATACCCTAGATAATGATTGGTTTTAATTGTTTTTAGCATCTGTTCCTTTTCTATCCCCATTCACCGCTTGtt                                                                                          >  1:1151402/1‑141 (MQ=255)

                                              cTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGtt                                                                                          >  1:955049/1‑141 (MQ=255)

                                              cTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGATTTTTATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGtt                                                                                          >  1:934867/1‑141 (MQ=255)

                                               ttCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCGTCTGTTAATTTTCTCTCACTGTTCAACGCTTGttt                                                                                         >  1:101682/1‑141 (MQ=255)

                                               ttCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGttt                                                                                         <  2:377000/141‑1 (MQ=255)

                                                tCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCAGCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTc                                                                                        <  1:206747/141‑1 (MQ=255)

                                                 cAGCTACAGTTTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCg                                                                                       <  2:1189541/141‑1 (MQ=255)

                                                 cAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATACAACGCTTGTTTCg                                                                                       <  1:630012/141‑1 (MQ=255)

                                                  aGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGt                                                                                      >  2:968198/1‑141 (MQ=255)

                                                   gCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTa                                                                                     >  1:199755/1‑141 (MQ=255)

                                                      aCAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgtt                                                                                  <  2:1073289/141‑1 (MQ=255)

                                                       taGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgttg                                                                                 <  1:351902/140‑1 (MQ=255)

                                                        aGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTCGTTTTCATTGTTTTTAGCATATGAAACGTTTCTATCACCATTCAACGCTTGTTTCGTAgttgt                                                                                >  1:814738/1‑141 (MQ=255)

                                                         gtatgCTCTTGATTGATGTCTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgttttt                                                                               >  2:738209/4‑141 (MQ=255)

                                                             gcgcTTGATTGATGTTTCCAGTAACAGCACATTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTTAACGCTTGTTTCGTAGTTGTTACat                                                                           >  2:497932/1‑140 (MQ=255)

                                                                cTTGATTGATGTTTCCAGTAACAGCACCTTTTTTTTTTTTTTTTAAGTTAACAAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCGTGTTTCGTAGTTGTTACAGTTg                                                                        >  2:1184111/1‑141 (MQ=255)

                                                                cTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTTTTTTTTTTAAGTTATCTAACGGAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTTCTTTTCTATCTCCATTCAACGCTTGTTTCGTAGTTGTTACAGTTg                                                                        >  2:964631/1‑141 (MQ=255)

                                                                cTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCAGTGTTTAAGTTATGTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTGTCACCATTCAAGGCGTGTTTCGTAGTTGTTACAGTTg                                                                        >  1:620898/1‑141 (MQ=255)

                                                                  tgattgatGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGaa                                                                      <  2:945618/141‑1 (MQ=255)

                                                                       gatGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGtgc                                                                 <  1:788925/141‑1 (MQ=255)

                                                                        atGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTACTTGTTACAGTTGTAAGtgct                                                                <  1:754810/141‑1 (MQ=255)

                                                                         tGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTGGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGtgctg                                                               >  1:939341/1‑141 (MQ=255)

                                                                           tttCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTgctgct                                                             <  1:904083/141‑1 (MQ=255)

                                                                                aGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAAc                                                        >  2:734689/1‑141 (MQ=255)

                                                                                  tAACAGCACGTTTTTGTGCATTGTTTAAGTTATCTAACGTAGCTAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATGCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACtt                                                      <  1:1005859/141‑1 (MQ=255)

                                                                                    aCAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgt                                                    <  2:14664/141‑1 (MQ=255)

                                                                                    aCAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgt                                                    >  1:191025/1‑141 (MQ=255)

                                                                                     cAGCACCTTTTTTTTCTTTTTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgtg                                                   >  1:1039950/1‑141 (MQ=255)

                                                                                     cAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCTTTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgtg                                                   <  2:164625/141‑1 (MQ=255)

                                                                                     cAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgtg                                                   >  2:153729/1‑141 (MQ=255)

                                                                                       gCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGAt                                                 >  2:196565/1‑141 (MQ=255)

                                                                                         aCCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTCCAGTTGAAAGTGCTGCTTCAACTTGTGATTg                                               >  2:764190/1‑141 (MQ=255)

                                                                                         aCCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAAGTTGTGATTg                                               >  2:664119/1‑141 (MQ=255)

                                                                                            tttttGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGtt                                            <  1:1024431/141‑1 (MQ=255)

                                                                                               ttGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGc                                         <  2:198435/141‑1 (MQ=255)

                                                                                               ttGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTGTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGc                                         >  2:888873/1‑141 (MQ=255)

                                                                                                tgtgCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCa                                        >  1:1065009/1‑141 (MQ=255)

                                                                                                 gtgCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGtttggat                                       >  2:387537/1‑136 (MQ=255)

                                                                                                 gtgCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCAt                                       >  1:426022/1‑141 (MQ=255)

                                                                                                     tttGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCGTCTGTTACTTTTCTATCACTATTCCACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGTTGCTTCAACTTGTGATTGGTTCGCCTTTGt                                   <  2:261642/140‑1 (MQ=255)

                                                                                                        ttttAAGTTCTCTACCGTAGGTAATGTTTGGTTTTTATTGTTTTTCGCATCTGTTACTTTTCTATCCCCATTCAACGCTTCTTTCGTTGTTGTTACAGTTGACAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTAcc                                <  2:609439/140‑1 (MQ=255)

                                                                                                         tttAAGTTTTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCa                               <  2:555284/141‑1 (MQ=255)

                                                                                                         tttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTATCATGCGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCa                               <  2:821164/141‑1 (MQ=255)

                                                                                                         tttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCa                               <  1:459889/141‑1 (MQ=255)

                                                                                                         tttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCa                               <  2:648601/141‑1 (MQ=255)

                                                                                                         tttAACTTGTCTACCGTAGATAATTTTTGGTTTTCATTGTTTTCCGCATCTGTTACTTTTGTATCCCCACTCAATGCCTGCGTCGTAGTTGTTTCAGTTGGACGTTCTGCGTCAACTTGTGATTGGTTCGCATTTGTACCa                               <  2:252860/141‑1 (MQ=255)

                                                                                                          ttAAGTTTTCTAACGTAGATAATTTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTTCTTCAACTTGTGATTGGTTCGCATTTGTACCAt                              <  2:790117/141‑1 (MQ=255)

                                                                                                          ttAAGTTATCTAACGTAGTTAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAt                              <  2:37546/141‑1 (MQ=255)

                                                                                                          ttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAt                              <  2:379703/141‑1 (MQ=255)

                                                                                                          ttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAt                              <  1:458810/141‑1 (MQ=255)

                                                                                                          ttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTATTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAt                              <  2:699602/141‑1 (MQ=255)

                                                                                                          ttAAGTTATCTAACGTAGATAATGTTTGGCTTTAATTGGTTTTATGCTCTATTACTTTTCTATCACCATTCAACGCTTTTTTCTGAGTGTGTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAt                              <  2:400991/141‑1 (MQ=255)

                                                                                                           tAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTCCAGTTGAAAGTGCTGCTTCAACTTGTGCTTGGTTCACCTTTGTACCAtt                             >  1:540422/1‑141 (MQ=255)

                                                                                                           tAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAtt                             >  1:470333/1‑141 (MQ=255)

                                                                                                             aggtaattAACGTTGAAAATGTTTGGTTTTCATTGTTTTCACCATCTGTTAATTTTCTATCACCATTCCACGCTTGTTTCGAAGTTGTTACAGTTGAAAGTGCTGCTTAAACTTGTGATTGGTTCGCATTTGTACCATTTg                           <  1:76115/134‑1 (MQ=255)

                                                                                                               ttttCTAAAGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGTTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCt                         <  2:392398/138‑1 (MQ=255)

                                                                                                                 aTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACTATTCAACGCTTGTTTCGTAGTTGTTGCAGTTGAAAGTACTGCTTCAACTTGTGATTGGTTCGCATTTGTACAATTTGCTTg                       >  2:597120/1‑141 (MQ=255)

                                                                                                                  tCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGa                      >  1:10063/1‑141 (MQ=255)

                                                                                                                  tCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGa                      >  2:138449/1‑141 (MQ=255)

                                                                                                                     aaCGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTGAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGCTta                   >  2:807032/1‑141 (MQ=255)

                                                                                                                          aGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTaataat              >  1:855897/1‑141 (MQ=255)

                                                                                                                           gATAATGTTTGGGTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTTTGATTGGTTCGCATTTGTACCATTTGCTTGATTAataata             >  1:852098/1‑141 (MQ=255)

                                                                                                                            aTAATGTTTGGTTTTCTTTGTTTTTAGCATCTGTTACTTGTCTATCACCATGCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAatat            <  2:232199/141‑1 (MQ=255)

                                                                                                                            aTAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAatat            <  2:625925/141‑1 (MQ=255)

                                                                                                                            aTAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAatat            <  1:106439/141‑1 (MQ=255)

                                                                                                                             ttattgttggttttCAGTGTTTTTAGTCTCTGTTTCTTGTCTATCACCATTTAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTTTGATTTGTTCGCCTTTGTACCATGTGCTTGATTTATAATAtt           <  2:596441/135‑1 (MQ=255)

                                                                                                                              aaTGTTTGGTTTTCATTGTTTTTAGCATTTGTTACCGTTCGAGCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAttt          <  1:929621/141‑1 (MQ=255)

                                                                                                                              aaTGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAttt          >  1:890814/1‑141 (MQ=255)

                                                                                                                              aaTGTTTGGTTTTCATTGTTTTTAGCATCTGTTAATTTTCTATGCCCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAttt          <  1:515681/141‑1 (MQ=255)

                                                                                                                              aaTGTTTGGTTTTCATTGTTTTGAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAttt          <  1:124692/141‑1 (MQ=255)

                                                                                                                                    tggttttCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTcagca    >  1:216198/1‑141 (MQ=255)

                                                                                                                                     ggttttCATTGTTTTTCGCATCTGTTACTTTTCTATCACCCTTTAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCCACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTcagcag   <  2:470333/141‑1 (MQ=255)

                                                                                                                                      gttttCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTcagcagc  <  2:1202114/141‑1 (MQ=255)

CCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGC  >  USA300TCH1516_ALE/1481217‑1481491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`