BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	367,644	G→T	intergenic (‑96/‑62)	nanA ← / → bglK	N‑acetylneuraminate lyase/Beta‑glucoside kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	367,644	0	G	T	100.0%	395.2 / NA	119	intergenic (‑96/‑62)	nanA/bglK	N‑acetylneuraminate lyase/Beta‑glucoside kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (67/52); total (67/52)

Only 100 of 119 total aligned reads displayed.

ACGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTAGTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATGTTT  >  USA300TCH1516_ALE/367508‑367780

aCGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCat                                                                                                                                      >  2:891850/1‑141 (MQ=255)

aCGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCat                                                                                                                                      >  2:858741/1‑141 (MQ=255)

 cGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAACTTGTATTCata                                                                                                                                     >  1:13847/1‑141 (MQ=255)

      aaCGCTGCATATAAACCTTTTAAATCTTTGTTCATGATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTAt                                                                                                                                >  1:580934/1‑141 (MQ=255)

       aagCTGCATATCAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATg                                                                                                                               <  2:83382/139‑1 (MQ=255)

       aCGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTGTTGAAAATTGTATTCATATTTATg                                                                                                                               <  1:773109/141‑1 (MQ=255)

        cGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGt                                                                                                                              <  1:194297/141‑1 (MQ=255)

        cGCTGCACATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGt                                                                                                                              <  1:194007/141‑1 (MQ=255)

        agCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCTTTCTCAAAGTAATTTGATAATATTGTTTAATGCTGTAATTGTAAGCGCTATCATAAAATG‑AAACAATATCTTTTTGAAAATTGTATTCATTTTTATGt                                                                                                                              <  2:503135/140‑1 (MQ=255)

             cATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAtaat                                                                                                                         >  1:688193/1‑141 (MQ=255)

             cATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAACA‑TAACAATATCGTTTTGAAAATTGTATTCATATTTATGTAtaat                                                                                                                         >  1:221246/1‑141 (MQ=255)

                ataAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTCTACTTGTCAACGGGA‑CGTAAAAAGATAACAAGAGCTTGTTGAAAATTGTATTCATATTTATGTAtaattaa                                                                                                                      >  2:1160043/1‑141 (MQ=255)

                     ccTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGaaa                                                                                                                 <  2:106811/141‑1 (MQ=255)

                          tAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTACAATTAATGAAAATGtt                                                                                                            <  1:713686/141‑1 (MQ=255)

                           aaaTCTTTGTTCATTATGAATTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGttt                                                                                                           <  1:104066/141‑1 (MQ=255)

                           aaaTCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATACAATT‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGttt                                                                                                           >  2:368528/1‑141 (MQ=255)

                           aaaTCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGttt                                                                                                           <  1:855762/141‑1 (MQ=255)

                           aaaTCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGttt                                                                                                           <  1:133753/141‑1 (MQ=255)

                           aaaTCCTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGttt                                                                                                           <  1:377444/141‑1 (MQ=255)

                            aaTCTTTGTTCATTATGAAGTCCATCCAAACATCATTTGATAATATAGTTTACAGCTATACTTGTAAACGCTATCATAAAATG‑TAACAATCTCTTTTTGATAATTGTATTCATATTTATGTATAATTAATGAAAATGtttt                                                                                                          >  2:532951/1‑141 (MQ=255)

                                       aTTATGAAGTCCCTCGAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAAt                                                                                               >  2:433313/1‑141 (MQ=255)

                                       aTTATGAAGTCCCTCCCAAAATCATTTGATACTATAGTTTACAGCTATAATCGTAAACGCTATCATAAAAGG‑TAACAAGATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGTAAATGTTTTTCAAAATCAAt                                                                                               >  2:832435/1‑141 (MQ=255)

                                            gAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGaaa                                                                                          >  1:950297/1‑141 (MQ=255)

                                            gAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTCGGGTTAGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGaaa                                                                                          >  1:1185680/1‑141 (MQ=255)

                                              aGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATTAATAGAAATg                                                                                        >  2:763925/1‑141 (MQ=255)

                                                     ggaaaaaTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAATAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtgag                                                                                 <  2:1207130/139‑1 (MQ=255)

                                                     ccAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAACATGTTTTTCAAAATCACTAGAAATGgagtgag                                                                                 >  1:806795/1‑141 (MQ=255)

                                                     ccAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtgag                                                                                 >  1:1169875/1‑141 (MQ=255)

                                                     ccAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtgag                                                                                 <  2:148741/141‑1 (MQ=255)

                                                     ccAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTAATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATgaaatcaa                                                                                 >  1:562303/1‑134 (MQ=255)

                                                         aaaTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAg                                                                             >  1:766612/1‑141 (MQ=255)

                                                              ttttGATAATATAGTTTAAAGCTATAATTGTAAACGCTATCATAAAATT‑GAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCATAATCAATAGAAATTGAGTGAGTAAGTTGTa                                                                        <  2:732187/140‑1 (MQ=255)

                                                                 tGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTa                                                                     <  1:482930/141‑1 (MQ=255)

                                                                 tGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTa                                                                     <  1:1109961/141‑1 (MQ=255)

                                                                     aataTAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATc                                                                 <  1:108368/141‑1 (MQ=255)

                                                                      atttaGTTTACAGCCATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCg                                                                <  2:647133/138‑1 (MQ=255)

                                                                      atatatTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTGTGAAAATGGTATTCATATTGATGTATAATTAATGAAAATTTTTTTCAAATTCAATAGAAATTGGTTGAGTATGTTGTATTACATct                                                                <  2:316198/141‑2 (MQ=255)

                                                                       tataGTTTACAGCTATAATTGTAAACTCTATCAGAAAATG‑TAAAGAACTGGAAGTGAAAATTGTATTCATATTTATTGATAATTAATTAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGc                                                               <  1:272116/141‑1 (MQ=255)

                                                                        ataGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAATATTAATTGAAATGGAGTGAGTAAGGTGTATTTCATCgaa                                                              >  2:52245/1‑139 (MQ=255)

                                                                        ataGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCa                                                              >  2:82004/1‑141 (MQ=255)

                                                                           gTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATc                                                           <  2:269371/141‑1 (MQ=255)

                                                                           gTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATc                                                           <  2:1088932/141‑1 (MQ=255)

                                                                           gTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATc                                                           >  2:26937/1‑141 (MQ=255)

                                                                            tttACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAATAGTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCATAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCg                                                          <  2:660500/141‑1 (MQ=255)

                                                                            tttACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATca                                                          >  2:265948/1‑140 (MQ=255)

                                                                             ttACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGa                                                         >  2:622367/1‑141 (MQ=255)

                                                                             ttACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGa                                                         >  2:129546/1‑141 (MQ=255)

                                                                               aCAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAAGATTTTTCAAAATCAATCGAAATTAAGTGTGTCAGGAGGTATACATCGCacacggta                                                       >  1:1052165/1‑134 (MQ=255)

                                                                                 aGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATAtt                                                     >  1:156480/1‑141 (MQ=255)

                                                                                 aGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATAtt                                                     >  1:201944/1‑141 (MQ=255)

                                                                                  gCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTTTTAATTGTTTTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAGTCAATAGAAATGGGGTGATTAAGGTGTATTACATCGCAATCGacatat                                                    >  2:474519/1‑139 (MQ=255)

                                                                                  gCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTTCATAGCAATCGatatag                                                    >  2:193029/1‑139 (MQ=255)

                                                                                   cTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTAAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTAACTCGAAATCGATATTgg                                                   >  1:442854/1‑141 (MQ=255)

                                                                                    tataATTGTAAACGCTATCATAAACTG‑TAACAATATCTATTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGa                                                  >  1:772989/1‑141 (MQ=255)

                                                                                    tataATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTggg                                                  >  1:735275/1‑140 (MQ=255)

                                                                                    tataATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTAAAAATCAATAGGAATGGAGTGAGTAAGGAGTATTACATCGCAATCGatatggag                                                  >  1:36324/1‑137 (MQ=255)

                                                                                    tataACTGTAACCGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTATATCCATATTTATGTATAATTAATGAAAAGGTATTTCAAAATAAATAGAAATGGAGTGCGTAAGGCGTATTAAATCGCAAGCGATATTGGa                                                  >  2:511830/1‑141 (MQ=255)

                                                                                      taATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAgg                                                <  2:767033/141‑1 (MQ=255)

                                                                                        aTTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCa                                              <  1:1081020/141‑1 (MQ=255)

                                                                                        aTTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGGGAGTAAGGTGTATTACATCGCGATCTATATTGAAGTCa                                              >  2:64864/1‑141 (MQ=255)

                                                                                              aaCGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCaaa                                        >  1:357839/1‑141 (MQ=255)

                                                                                              aaCGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCaaa                                        >  1:56322/1‑141 (MQ=255)

                                                                                                  cTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTaa                                    <  2:946758/141‑1 (MQ=255)

                                                                                                  cTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGAAATTGGAGGCACCCAAATTaa                                    >  2:269181/1‑141 (MQ=255)

                                                                                                     tCATAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATc                                 >  2:336590/1‑141 (MQ=255)

                                                                                                       agaaaaTG‑TAACAATATCTTTTTGATAATTTTATTCATAGTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAACGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCgg                               <  2:442854/139‑1 (MQ=255)

                                                                                                       aTAAAATG‑TAACAATATCTTTTTTAATTTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAAGGGAGTGAGTAAGGTGTATTACATCGCAATCAATATTGGAGGCACCCAAATTAAAttgt                               >  2:459589/1‑138 (MQ=255)

                                                                                                        tAAAATG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGc                              <  2:804222/141‑1 (MQ=255)

                                                                                                        aaaaaTG‑TAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACACCGCAATCGATATTGGAGGCACTCAAATTAAATCGGc                              <  2:264088/140‑1 (MQ=255)

                                                                                                         aaaaTG‑TAATAAAATCTTTTTTAAATTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCa                             <  2:512327/141‑1 (MQ=255)

                                                                                                                 aaCAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTg                      >  1:8955/1‑141 (MQ=255)

                                                                                                                  aCAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGa                     <  1:891850/141‑1 (MQ=255)

                                                                                                                   cAATATCTTTTTGAAAATTGTATTCATATTTGTGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGGGGCACTCAAATTAAATCGGCAGTTATTGAt                    <  2:511269/141‑1 (MQ=255)

                                                                                                                   cAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGAt                    >  2:457954/1‑141 (MQ=255)

                                                                                                                    aaTATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATa                   <  2:1163746/141‑1 (MQ=255)

                                                                                                                    aaTATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATa                   >  2:769315/1‑141 (MQ=255)

                                                                                                                    aaTATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATa                   >  2:769473/1‑141 (MQ=255)

                                                                                                                     atatCTTTTTGAAATTTATATTCATATTTATGTACAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATaa                  <  2:866769/141‑1 (MQ=255)

                                                                                                                     atatCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTAGTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATaa                  <  2:1190910/141‑1 (MQ=255)

                                                                                                                        tCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGTAGTTATTGATAAGCa               <  1:895284/141‑1 (MQ=255)

                                                                                                                        tCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCa               <  1:769315/141‑1 (MQ=255)

                                                                                                                         cTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAAGAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCCCTCAAATTAAATCGGAAGTTATTGATAATCaa              >  2:809238/1‑141 (MQ=255)

                                                                                                                           ttttGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAAtt            >  1:428405/1‑141 (MQ=255)

                                                                                                                           ttttGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAAtt            >  1:646365/1‑141 (MQ=255)

                                                                                                                            tttGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAAtgg           >  1:82568/1‑139 (MQ=255)

                                                                                                                            tttGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTg           >  1:971380/1‑141 (MQ=255)

                                                                                                                             ttGAAAATTGTATTCATATGTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAGTGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGa          >  2:11145/1‑141 (MQ=255)

                                                                                                                              tGAAAATTGTATTTATATTGATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGaa         <  1:832435/141‑1 (MQ=255)

                                                                                                                              tGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCTCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATGGATAAGCAATTGaa         <  1:629409/141‑1 (MQ=255)

                                                                                                                              tGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGaa         <  1:433313/141‑1 (MQ=255)

                                                                                                                              tGAAAATTGTATTCATATTTATGGATAATTAATGAAAATGGTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGAGATTGGAGGCACGCAAATTAAATCGGCAGTTATTGATAAGCAATTGaa         <  2:946119/141‑1 (MQ=255)

                                                                                                                               gAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAat        <  2:156480/141‑1 (MQ=255)

                                                                                                                               gAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAACCGATATTGGAGGCACTCAAATTACCTCGGCAGTTATTGATAAGCAATTGAat        <  2:580934/141‑1 (MQ=255)

                                                                                                                                aaaaTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAata       >  1:517678/1‑141 (MQ=255)

                                                                                                                                aaaaTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAata       <  2:342280/141‑1 (MQ=255)

                                                                                                                                aaaaTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAata       >  1:120170/1‑141 (MQ=255)

                                                                                                                                aaaaTTGTATTAATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATAGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAata       <  1:370142/141‑1 (MQ=255)

                                                                                                                                  aaTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATg     >  1:484884/1‑141 (MQ=255)

                                                                                                                                   aTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATGt    >  1:1053798/1‑141 (MQ=255)

                                                                                                                                   aTTGTATTCATATTGATGTATAATTAATGAAAATGTTTGTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATGt    <  2:412826/141‑1 (MQ=255)

ACGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATG‑TAACAATATCTTTTTGAAAATTGTAGTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATGTTT  >  USA300TCH1516_ALE/367508‑367780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`