BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	479,553	G→A	A194T (GCA→ACA)	USA300HOU_RS02280 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	479,553	0	G	A	98.3%	361.3 / NA	119	A194T (GCA→ACA)	USA300HOU_RS02280	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (55/61); minor base C (0/2); total (55/64)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

AGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTA  >  USA300TCH1516_ALE/479415‑479686

aGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGAACAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGGAAAG‑TTTTTACcataca                                                                                                                                     >  2:208336/1‑141 (MQ=255)

 gTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGATTTTTACcataca                                                                                                                                     >  2:364507/1‑141 (MQ=255)

     tGCAATAATAGATGAACAAGGTGAGCCGCTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGCtt                                                                                                                                >  2:463768/1‑141 (MQ=255)

     tGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGtt                                                                                                                                >  2:624530/1‑141 (MQ=255)

     tGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTACGATCTAGTTGAACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACCTGGtt                                                                                                                                >  2:1097155/1‑141 (MQ=255)

      gCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTg                                                                                                                               <  2:301358/141‑1 (MQ=255)

      gCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑GTTTTACCATACATGGTTg                                                                                                                               >  1:659805/1‑141 (MQ=255)

              aGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAgc                                                                                                                       <  1:963120/141‑1 (MQ=255)

               taTGAACAAGGTTAGCCACTTAGCAAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTCTCGAGTTGGACAATGCCCAAGCGTGAGCAAAG‑TTTTTACCATACATGCTTGCATTTAgcg                                                                                                                      <  2:437153/140‑1 (MQ=255)

               gATGAACAATGCGAGCCACGTAGCGACCAAGTCAACCGGCAAATGATTAAAGGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAgcg                                                                                                                      <  2:437283/141‑1 (MQ=255)

               gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCTAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAgcg                                                                                                                      <  2:638447/141‑1 (MQ=255)

               gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGCCGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAgcg                                                                                                                      >  1:801075/1‑141 (MQ=255)

               gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGTGTTTTTCTATGCCGAAGCCTCAGCAAAG‑TTTTTACCATACATGGTTGCATTTAgcg                                                                                                                      <  2:547835/141‑1 (MQ=255)

               gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAgcg                                                                                                                      <  1:1039314/141‑1 (MQ=255)

               gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAATTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTGGCATTTAgcg                                                                                                                      >  1:494690/1‑141 (MQ=255)

                aTGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAgcgc                                                                                                                     <  1:1078362/141‑1 (MQ=255)

                   aaCAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAAc                                                                                                                  <  1:290540/141‑1 (MQ=255)

                    aCAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACa                                                                                                                 <  2:150171/141‑1 (MQ=255)

                    aCAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACACGGTTGCATTTAGCGCAACa                                                                                                                 <  1:899408/141‑1 (MQ=255)

                     cAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACAt                                                                                                                >  2:24485/1‑141 (MQ=255)

                      aaGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATg                                                                                                               >  2:1180198/1‑141 (MQ=255)

                      aaGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATg                                                                                                               <  2:618524/141‑1 (MQ=255)

                       agttGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGa                                                                                                              <  2:267914/138‑1 (MQ=255)

                       aGGTGAGCCACTTAGCGCACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGAACAATTTCTATCGAGTGGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCaaactga                                                                                                              >  2:554344/1‑136 (MQ=255)

                       aGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGa                                                                                                              >  2:945958/1‑141 (MQ=255)

                       aGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGa                                                                                                              >  1:913631/1‑141 (MQ=255)

                        ggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGGGCCAGATGAc                                                                                                             >  2:30492/1‑141 (MQ=255)

                        ggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGAc                                                                                                             >  2:958320/1‑141 (MQ=255)

                        ggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGAc                                                                                                             >  1:762139/1‑141 (MQ=255)

                        ggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGAc                                                                                                             >  1:890593/1‑141 (MQ=255)

                        ggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGAc                                                                                                             >  1:523320/1‑141 (MQ=255)

                        ggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGAc                                                                                                             >  2:804499/1‑141 (MQ=255)

                         gTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTGGCGCAACATGAcc                                                                                                            >  1:986578/1‑141 (MQ=255)

                         gTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGAcc                                                                                                            >  2:381691/1‑141 (MQ=255)

                          cgAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCa                                                                                                           <  1:47656/140‑1 (MQ=255)

                             gCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAg                                                                                                        >  1:1033145/1‑141 (MQ=255)

                              ccACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGt                                                                                                       >  1:397906/1‑141 (MQ=255)

                                       gAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTaaa                                                                                              <  2:65528/141‑1 (MQ=255)

                                        aaCAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAg                                                                                             <  2:469363/141‑1 (MQ=255)

                                           aaGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGcac                                                                                          <  1:971916/141‑1 (MQ=255)

                                             gTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAg                                                                                        >  2:47655/1‑141 (MQ=255)

                                               gAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcg                                                                                      >  1:1010070/1‑141 (MQ=255)

                                                 aTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCAGAGCGcc                                                                                    >  2:921379/1‑141 (MQ=255)

                                                   cGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTTAAGCACAGCGCCaa                                                                                  >  2:622303/1‑141 (MQ=255)

                                                   cGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCaa                                                                                  <  1:1158599/141‑1 (MQ=255)

                                                    gTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAATCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACGATAGTTTTACTAAAACACAACGCCacc                                                                                 >  2:226967/1‑139 (MQ=255)

                                                    gTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAg                                                                                 >  2:870451/1‑141 (MQ=255)

                                                     ttaaaTGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTCGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGt                                                                                <  2:788757/139‑1 (MQ=255)

                                                     tCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGt                                                                                <  2:884938/141‑1 (MQ=255)

                                                      cAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGTGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTg                                                                               <  2:130742/141‑1 (MQ=255)

                                                          cgATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTa                                                                           <  1:831943/140‑1 (MQ=255)

                                                           gATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTCTACTAAAGCACAGCGCCAAGTGATTaa                                                                          >  1:974174/1‑141 (MQ=255)

                                                            aTTAAATGGACGAAACCTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTaaa                                                                         <  2:15886/141‑1 (MQ=255)

                                                              tAAATGGACGAAACTTTATATCGATCAATTTCTCTCGAGTTGGACAATGCCGAAGCGTGAGCCAAG‑TTTTTACCATACATGGTTGCATTTAGCGCACCATGACCATAGTTTTAATCAAGCACAGCGCCAAGTGATTAAAgg                                                                       >  2:299232/1‑141 (MQ=255)

                                                              tAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGGTTAAAgg                                                                       >  1:257156/1‑141 (MQ=255)

                                                              tAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAgg                                                                       <  1:505448/141‑1 (MQ=255)

                                                                   ggACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTAc                                                                  <  1:1180198/141‑1 (MQ=255)

                                                                          aCTTTATATCGATCAATTTCTCACAGCTTTGACAAGGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGa                                                           >  1:1198037/1‑141 (MQ=255)

                                                                          aCTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGa                                                           >  1:756162/1‑141 (MQ=255)

                                                                           cTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGAt                                                          >  2:748994/1‑141 (MQ=255)

                                                                            tttATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATc                                                         <  2:110963/141‑1 (MQ=255)

                                                                            tttATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATc                                                         <  1:1095894/141‑1 (MQ=255)

                                                                            tttATATCGATCAATTTCGATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATc                                                         <  2:1146490/141‑1 (MQ=255)

                                                                             ttATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACAATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATcc                                                        >  1:359647/1‑141 (MQ=255)

                                                                                  tCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTgcac                                                   <  2:913631/141‑4 (MQ=255)

                                                                                        aaTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGa                                             <  1:146249/141‑1 (MQ=255)

                                                                                        aaTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGa                                             >  2:955312/1‑141 (MQ=255)

                                                                                        aaTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGa                                             >  1:181921/1‑141 (MQ=255)

                                                                                        aaTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGa                                             >  2:970788/1‑141 (MQ=255)

                                                                                        aaTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGa                                             <  1:935048/141‑1 (MQ=255)

                                                                                         aTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGAt                                            <  1:605480/141‑1 (MQ=255)

                                                                                          tttCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATa                                           >  2:761716/1‑141 (MQ=255)

                                                                                           ttCTCTCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATGGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATag                                          <  2:489229/141‑1 (MQ=255)

                                                                                             cTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGCCGATagag                                        >  1:932283/1‑141 (MQ=255)

                                                                                              tATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATCCTTGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACTGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGt                                       <  2:1158951/141‑1 (MQ=255)

                                                                                              tATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGt                                       >  2:1107827/1‑141 (MQ=255)

                                                                                                 cGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCCTTATGGGGAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAg                                    <  1:570208/141‑1 (MQ=255)

                                                                                                 cGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAg                                    <  2:1008481/141‑1 (MQ=255)

                                                                                                  gAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTGTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGGCAgg                                   >  1:863728/1‑140 (MQ=255)

                                                                                                   aGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCACGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAATCAGtg                                  >  1:29036/1‑140 (MQ=255)

                                                                                                    gTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTAt                                 <  1:320908/141‑1 (MQ=255)

                                                                                                    gTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTAt                                 <  2:1192213/141‑1 (MQ=255)

                                                                                                        gACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAAc                             <  1:47655/141‑1 (MQ=255)

                                                                                                        gACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAAc                             <  1:381691/141‑1 (MQ=255)

                                                                                                          cAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTc                           <  1:24485/141‑1 (MQ=255)

                                                                                                            aTGCCGAAGCGGGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTACGCATTAACTCAt                         >  2:187314/1‑141 (MQ=255)

                                                                                                              gCCGAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttt                       >  1:399134/1‑141 (MQ=255)

                                                                                                                 gAAGCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGTCGATAGAGTCAGTATTAACTCATTTTTc                    >  1:838633/1‑141 (MQ=255)

                                                                                                                    gCGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAAt                 <  2:523973/141‑1 (MQ=255)

                                                                                                                     cGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATa                <  1:718501/141‑1 (MQ=255)

                                                                                                                     cGTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATa                <  1:945958/141‑1 (MQ=255)

                                                                                                                      gTGAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAg               <  2:181921/141‑1 (MQ=255)

                                                                                                                        gAGCAAAG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGAt             >  1:441212/1‑141 (MQ=255)

                                                                                                                            aaaG‑TTTTTCCAATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGACTCCATGCTAACTGATTTTTCTATAGATCAgg         <  2:552512/141‑1 (MQ=255)

                                                                                                                              aG‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATTCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGaa       <  2:890593/141‑1 (MQ=255)

                                                                                                                               g‑TTTTTGCCATGCATGGTTGCATTTCGCGCAACATGACCAGTGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACGCAGTTTTCAATAGATCAGGAAg      <  2:563291/141‑1 (MQ=255)

                                                                                                                               g‑TTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAg      <  1:748994/141‑1 (MQ=255)

                                                                                                                                  ttttACCATACATGGTTTCATTTAGCGCAACATGACCATAGTTTGACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAACAGATCAGGAAGAc    <  2:44952/141‑1 (MQ=255)

                                                                                                                                  ttttACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGAc    <  2:762139/141‑1 (MQ=255)

                                                                                                                                    ttACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTa  >  1:70738/1‑141 (MQ=255)

AGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAG‑TTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTA  >  USA300TCH1516_ALE/479415‑479686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`