BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	638,614	G→T	T14K (ACA→AAA)	pdxK ←	Pyridoxine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	638,614	0	G	T	100.0%	425.6 / NA	130	T14K (ACA→AAA)	pdxK	Pyridoxine kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (50/80); total (50/80)

Only 100 of 133 total aligned reads displayed.

CATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAATG  >  USA300TCH1516_ALE/638477‑638751

taTCCCTTTGTAATGGTGTAACATCGTTTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTttgt                                                                                                                                        <  2:295626/140‑1 (MQ=255)

cttCCATTGGATATGTTGGAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGGTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTttgt                                                                                                                                        <  2:699990/139‑1 (MQ=255)

cATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTttgt                                                                                                                                        <  2:403479/141‑1 (MQ=255)

     cttGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGaa                                                                                                                                   <  1:522525/140‑1 (MQ=255)

      ttGGTAATGGTGTAACGTTGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAAc                                                                                                                                  <  1:587745/141‑1 (MQ=255)

      ttGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAAc                                                                                                                                  <  2:418671/141‑1 (MQ=255)

        ggTAATGGTGTAACTTCGTGTTACCATTTATCGTTATCCATAGTAACGATGGCCGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCg                                                                                                                                <  2:166109/141‑1 (MQ=255)

             tGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAAt                                                                                                                           >  2:1073505/1‑141 (MQ=255)

               gtgtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTg                                                                                                                         <  2:770646/141‑1 (MQ=255)

                 gtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGtt                                                                                                                       <  2:129840/141‑1 (MQ=255)

                   aaCATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCCGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTaa                                                                                                                     <  1:92571/141‑1 (MQ=255)

                   aaCATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTTTTaa                                                                                                                     <  1:823203/141‑1 (MQ=255)

                   aaCATCGTGTGACCATGCATCTTTATCCATAGTAACGATGGAAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTaa                                                                                                                     <  1:222142/141‑1 (MQ=255)

                       tCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATCGGTAAAAct                                                                                                                 >  2:1079076/1‑141 (MQ=255)

                        cGTGTGACCATGTATCTTTTTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActt                                                                                                                <  1:768585/141‑1 (MQ=255)

                        cGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTACTTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActt                                                                                                                >  2:76146/1‑141 (MQ=255)

                          tgtgACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActttc                                                                                                              <  2:197289/141‑1 (MQ=255)

                           gtgACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActttct                                                                                                             >  1:785827/1‑141 (MQ=255)

                           gtgACCATGTATCTGTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTCCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActttct                                                                                                             <  2:64604/141‑1 (MQ=255)

                            tgACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActttctt                                                                                                            >  1:1147644/1‑141 (MQ=255)

                               ccATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGTCTATACTCTCCAGCACTTTTGTCAGAACCGTCAATTGTTAAAACTTTCTTTaa                                                                                                         >  1:871662/1‑141 (MQ=255)

                                 ctGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCTGCAATTGTTTAAACTTTCTTTAAAg                                                                                                       <  1:604352/140‑1 (MQ=255)

                                     aTCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCAt                                                                                                   <  1:916760/141‑1 (MQ=255)

                                      tCTTTATCCATAGTAACGACGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGGCAGCACTTTTGTCAGAACAGGAAAAAGTTCACACTTTCTTTAAAGCCAtt                                                                                                  <  2:886747/141‑1 (MQ=255)

                                       ttttATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTg                                                                                                 <  2:241284/140‑1 (MQ=255)

                                        tttgtgcATAGATACGATGGCAGTTAAAGCGAACCTGCAATACGTATCTAAATCTTGGAACGTTTTCAAATCTTCTTGCATACCTGCGTAAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGa                                                                                                <  1:103124/135‑1 (MQ=255)

                                         ttATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCCATTGCTAAAACTTTCTTTAAAGCCATTGAg                                                                                               <  2:256995/141‑1 (MQ=255)

                                         ttATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAg                                                                                               <  1:46347/141‑1 (MQ=255)

                                           aTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCt                                                                                             <  1:10265/141‑1 (MQ=255)

                                           aTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCt                                                                                             >  2:704425/1‑141 (MQ=255)

                                            tCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCTAAGCCGCCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCtt                                                                                            >  1:220700/1‑141 (MQ=255)

                                            tCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAACCCATTGAGCtt                                                                                            >  1:832267/1‑141 (MQ=255)

                                             ccATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTc                                                                                           <  2:1069347/141‑1 (MQ=255)

                                             ccATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTc                                                                                           <  2:8639/141‑1 (MQ=255)

                                             ccATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGGCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTc                                                                                           <  2:677287/141‑1 (MQ=255)

                                             ccATAGTAACGATGGCAGTAAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCCGCTCTTTTGTCAGAGCCGGCAATTCTTAAAACTTTCTTTAAAGCCATTGATCTTc                                                                                           <  2:731572/141‑1 (MQ=255)

                                              cATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACCTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCa                                                                                          <  1:691504/141‑1 (MQ=255)

                                                tAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACt                                                                                        <  1:545413/141‑1 (MQ=255)

                                                 aGTAACGATGGCATTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTc                                                                                       <  1:927845/141‑1 (MQ=255)

                                                       gATGGCAGTTAAAGCGACCATGCCATACTTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACat                                                                                 <  1:1121052/141‑1 (MQ=255)

                                                       aaTGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACat                                                                                 <  2:175890/140‑1 (MQ=255)

                                                        aTGGCAGTTAAAGCGACCATGCCATACGTATCTAATTATTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACata                                                                                >  2:837265/1‑141 (MQ=255)

                                                         tGGCAGTTAACGCAAAAATGCTATACGCATTTAAGTATTGGCACGTGTTCTAAACAACTAACATACCAGCTCCAGCACTTTTTGCAGTACCTGGAATTTTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataa                                                                               <  2:103444/141‑1 (MQ=255)

                                                          ggCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGATTAACTCCTACataat                                                                              >  2:1163170/1‑141 (MQ=255)

                                                           gCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGTACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataata                                                                             <  2:144611/141‑1 (MQ=255)

                                                           gCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAGGCCATTGAGCTTCCCTCCTACataata                                                                             >  2:961871/1‑141 (MQ=255)

                                                           gCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataata                                                                             >  2:961579/1‑141 (MQ=255)

                                                           gCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTAAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTTATTCCTCCataata                                                                             >  2:1208599/1‑141 (MQ=255)

                                                              gTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataata                                                                          <  1:218676/141‑1 (MQ=255)

                                                               ttAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAatat                                                                         <  1:478224/141‑1 (MQ=255)

                                                               ttAAAGAGACCCAGCCATACGTATTCAATGCCTGAAACTTTATCAGATTGGCTCGCAGACCTGCGCCAGCACTTTTGTCTGTACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAAGAatat                                                                         <  2:848472/141‑1 (MQ=255)

                                                                tAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATAtt                                                                        <  1:1005476/141‑1 (MQ=255)

                                                                tAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATAtt                                                                        <  1:186473/141‑1 (MQ=255)

                                                                  aaGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATGATATTGt                                                                      >  1:102356/1‑141 (MQ=255)

                                                                    gCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATACTATTGTAt                                                                    >  2:200657/1‑141 (MQ=255)

                                                                     ggACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTAtt                                                                   <  2:46223/140‑1 (MQ=255)

                                                                     cGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAACTCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAACTGTTAAAACTTTCTTTAAAGCCATTGCGCTTCACTCATACATAATAATATTGTAtt                                                                   >  2:298218/1‑141 (MQ=255)

                                                                       aCCATGCCATACGGATCTAGTTCTTCGAACGTTTTCAAATCTGCATGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTGCACTCCTACATAATAATATTGTAttac                                                                 >  1:659004/1‑139 (MQ=255)

                                                                         cATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATtcatc                                                               <  1:905767/141‑1 (MQ=255)

                                                                         cATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATtcatc                                                               <  1:86987/141‑1 (MQ=255)

                                                                          aTGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATtcatca                                                              <  2:1147644/141‑1 (MQ=255)

                                                                           tGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTcatcat                                                             >  1:117156/1‑141 (MQ=255)

                                                                           tGCCATACGTAGCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGTACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATACCAATATTGTATtcatcaa                                                             <  2:797958/141‑2 (MQ=255)

                                                                            gCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcata                                                            >  1:252508/1‑141 (MQ=255)

                                                                             ccATCCGCATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcatat                                                           <  1:684820/141‑1 (MQ=255)

                                                                              cATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATAATCGACCGCGGACTGTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcatatc                                                          <  1:1182425/141‑1 (MQ=255)

                                                                               aTACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcatatca                                                         <  1:1203477/141‑1 (MQ=255)

                                                                                   ttATTTANTTCTTGGAACGTTTTCAAATCTGCTTGCATACCGGCGCCAGCACCTTTGTCAGAACCGGAAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAtttt                                                     <  2:192371/140‑1 (MQ=255)

                                                                                   gTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAtttt                                                     <  2:347186/141‑1 (MQ=255)

                                                                                   gTATCTAAGTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAtttt                                                     <  1:594117/141‑1 (MQ=255)

                                                                                         aaTTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCt                                               <  1:559185/141‑1 (MQ=255)

                                                                                          aTTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTa                                              >  2:464784/1‑141 (MQ=255)

                                                                                             tttGGAACGTTGTCAAATCTGCTTGCATTCCTGCGCCACCACTTTTGTCAGAACCGGACATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAAAATTGTATTCATCATATCATTTTTAACCTAAtt                                           <  2:275246/140‑1 (MQ=255)

                                                                                             cTTGGATCGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAAtt                                           <  2:384156/141‑1 (MQ=255)

                                                                                               tGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGa                                         >  1:1171817/1‑141 (MQ=255)

                                                                                               tGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGa                                         >  2:448836/1‑141 (MQ=255)

                                                                                                gtaaCGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTTTCAGAACCGGCAATTGTTAAAACTTTCTTTACAGCCCTTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGaa                                        <  2:83058/139‑1 (MQ=255)

                                                                                                ggAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGaa                                        <  1:685180/141‑1 (MQ=255)

                                                                                                 gAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGaaa                                       <  2:621784/141‑1 (MQ=255)

                                                                                                   aCGTTTTCAAATCTGCTTGCATACCTGCGCCAGCTCTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGgaaaa                                     >  2:479457/1‑141 (MQ=255)

                                                                                                   aCGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGaaaaa                                     >  1:608740/1‑141 (MQ=255)

                                                                                                    cGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAat                                    >  2:953807/1‑141 (MQ=255)

                                                                                                     tttttCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAata                                   <  2:1180680/140‑1 (MQ=255)

                                                                                                     gTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAata                                   <  2:102356/141‑1 (MQ=255)

                                                                                                      ttttCAATTCTGCTTGCATACCTGCGCAAGCACTTTTATCAAAATCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTATATAATAATTGTGGATTCATCATATCATTTTTAAAATAAAAAAAAAatat                                  <  2:491603/141‑1 (MQ=255)

                                                                                                       tttCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATAtt                                 >  1:367490/1‑141 (MQ=255)

                                                                                                        ttCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTa                                <  2:446146/141‑1 (MQ=255)

                                                                                                           aaaTCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGGATTCATCATATCATTTTTAACCTAATTAAAAAATATTAAGc                             >  1:249300/1‑141 (MQ=255)

                                                                                                               cTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTc                         <  2:367490/141‑1 (MQ=255)

                                                                                                               cTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTc                         >  2:1007334/1‑141 (MQ=255)

                                                                                                                  cTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATACAat                      >  2:598915/1‑141 (MQ=255)

                                                                                                                      cATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATAttt                  >  1:481221/1‑141 (MQ=255)

                                                                                                                       atcccTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtg                 <  1:199340/138‑1 (MQ=255)

                                                                                                                           ctccgcCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATTTTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtgatga             <  2:648256/138‑1 (MQ=255)

                                                                                                                               gcCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTAATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGAttgt         >  2:270284/1‑141 (MQ=255)

                                                                                                                                ccAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGAttgtt        >  1:606385/1‑141 (MQ=255)

                                                                                                                                  aGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGa      >  1:817322/1‑141 (MQ=255)

                                                                                                                                   gCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGaa     <  1:407725/141‑1 (MQ=255)

                                                                                                                                   gCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGaa     <  1:1147461/141‑1 (MQ=255)

                                                                                                                                      cTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAATg  <  2:195415/141‑1 (MQ=255)

CATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAATG  >  USA300TCH1516_ALE/638477‑638751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`