BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	707,879	A→T	L11I (TTA→ATA)	znuC_1 ←	High‑affinity zinc uptake system ATP‑binding protein ZnuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	707,879	0	A	T	100.0%	307.3 / NA	99	L11I (TTA→ATA)	znuC_1	High‑affinity zinc uptake system ATP‑binding protein ZnuC
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (40/59); total (40/59)

Only 100 of 103 total aligned reads displayed.

TACCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGT  >  USA300TCH1516_ALE/707741‑708015

tACCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATTCTTATTACCTataa                                                                                                                                        >  1:407556/1‑141 (MQ=255)

 aCCGGTAGCATTAAATTCACCAATTAAAGACTTGATCAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCCCGTACTGGGATCGATAAGGAAATGTTTTTAAGTACATGCTAATTCCCTataac                                                                                                                                       >  1:103631/1‑140 (MQ=255)

 aCCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGCTAAGGAAATGTTTTTAAGTACATGCTTATTACCTataaa                                                                                                                                       >  1:892608/1‑141 (MQ=255)

 aCCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTataaa                                                                                                                                       >  1:75256/1‑141 (MQ=255)

  ccGGTCGCATTAAATTCACCTATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGTCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAAc                                                                                                                                      <  2:491000/141‑1 (MQ=255)

  ccGGTAGTATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGCAAATGTTTTTAAGTACATGCTTATTACCTATAAAc                                                                                                                                      <  2:398012/141‑1 (MQ=255)

    ggTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCCTGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTCCATGCTTATTACCTATAAACaa                                                                                                                                    >  2:292648/1‑140 (MQ=255)

     gTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGa                                                                                                                                   >  1:710624/1‑141 (MQ=255)

             aaaTTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATc                                                                                                                           <  2:833597/141‑1 (MQ=255)

               aTTCACCAATTAAAGACTTGATAAGGGAAGATTTAACAGCAGCATGCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCtt                                                                                                                         >  2:1102496/1‑141 (MQ=255)

                ttCACCGATTAAAGACTTGATAATGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCttt                                                                                                                        <  2:381610/141‑1 (MQ=255)

                  cACCAATTAAAGTCTTGATAAGGGAAGACTTAACAACACCATTAGGGCCCATGATACCAATTTTTACGCCACGCACGCGTATCGCATACGAGATTGTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTg                                                                                                                      <  1:282190/141‑1 (MQ=255)

                            aGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACaa                                                                                                            >  1:916551/1‑141 (MQ=255)

                                ttGATAATGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGt                                                                                                        <  1:599111/141‑1 (MQ=255)

                                ttGATAAGGGAAGATTTACCGGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGt                                                                                                        <  1:1032928/141‑1 (MQ=255)

                                 tGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGtt                                                                                                       >  2:387755/1‑141 (MQ=255)

                                 tGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTACAaaaaaatttt                                                                                                       >  2:1150791/1‑137 (MQ=255)

                                  gATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAACCGttt                                                                                                      >  2:708175/1‑141 (MQ=255)

                                   aTAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTta                                                                                                     >  1:527189/1‑141 (MQ=255)

                                       gggAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATAcc                                                                                                 <  2:403150/141‑1 (MQ=255)

                                        ggAAGATTTACCAGCACCATTCGGGCCCATGATACCAAGTATTTCGCCGTGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATAcct                                                                                                <  1:285188/141‑1 (MQ=255)

                                           aGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAAAAAACGTTTATAcctcct                                                                                             >  2:212907/1‑141 (MQ=255)

                                                  ccAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTaaa                                                                                      >  2:1142020/1‑141 (MQ=255)

                                                  ccAGCACCATTCGGGCCCATGATACCAATTATTTCACCGCGTACTGGTATCGAGAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTaaa                                                                                      >  1:762735/1‑141 (MQ=255)

                                                  ccAGCACCATTCAGGCCCATGATACCAATTATTTCGCCGCGGACGGCTATCGATAAGGAAACGTCATTAAGTACATGCTTATTACCTATAAACAGATGTAAGTGTTTTGTTTCTAACAAACGGTTGTACCTCCTACTTaaa                                                                                      >  1:850180/1‑141 (MQ=255)

                                                      cACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGtt                                                                                  <  2:464991/141‑1 (MQ=255)

                                                       cccATTCGGGCCCATGATACCAATTATATAGCCGCGTACTTGTATCGATAAGGAAATGTTTTTTAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGttt                                                                                 <  1:814405/140‑1 (MQ=255)

                                                          aTTCGGGCCCATTATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTCGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAgg                                                                              <  2:710624/141‑1 (MQ=255)

                                                            tCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTAACTATAAACAGATTTAAATATTTTGTTTCTAACAAACGTTTATACCTCCTCATTTAAAGTTTaaggt                                                                            >  1:614127/1‑137 (MQ=255)

                                                              gggCCCATGATTCCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTaa                                                                          <  1:1028269/141‑1 (MQ=255)

                                                              gggCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTaa                                                                          >  2:1183537/1‑141 (MQ=255)

                                                              gggCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTaa                                                                          >  2:933763/1‑141 (MQ=255)

                                                                gccactGACACCAATTATTTCGCCGCGTACTGGTATCGCTAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTGTGTTTCTAACAACCGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                        <  1:498517/136‑1 (MQ=255)

                                                                gCCCATGATACCAATTATTTCGCCGCGTCCTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTGAGGCTAGcc                                                                        <  2:558321/141‑1 (MQ=255)

                                                                gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                        <  2:75256/141‑1 (MQ=255)

                                                                gCCAATGATACCAATGATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                        <  2:480668/141‑1 (MQ=255)

                                                                    aTGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaat                                                                    <  1:1011573/141‑1 (MQ=255)

                                                                      gATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaatta                                                                  >  1:859169/1‑141 (MQ=255)

                                                                          ccAATTTTTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTg                                                              <  1:537646/141‑1 (MQ=255)

                                                                          ccAATTTTTTCGCCGCGTACTGCTATCGATAAGGAAATGTTTTCAAGTACATGCTTATTACCTTTAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTCAAAGTTTAGGCTAACCTAATTAATTg                                                              <  1:1050431/141‑1 (MQ=255)

                                                                          ccAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTg                                                              <  1:388277/141‑1 (MQ=255)

                                                                            aaTTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGta                                                            <  2:351921/141‑1 (MQ=255)

                                                                             attattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtat                                                           >  1:366451/1‑141 (MQ=255)

                                                                              ttattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtata                                                          <  2:1147481/141‑1 (MQ=255)

                                                                              ttagtTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtata                                                          <  2:809787/141‑1 (MQ=255)

                                                                               tattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAATTGTTTAGGCTAACCTAATTAATTGTataa                                                         >  2:844006/1‑141 (MQ=255)

                                                                               tattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTataa                                                         >  2:626351/1‑141 (MQ=255)

                                                                                 tttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACGTGGTTGGTACATATAAACAGATTTATATCTTTCTTTTCCTCGATACTCTGGAACCACTTTATGATAATTTTAGGCGAACCTAATTAATTTTataata                                                       >  2:252460/1‑141 (MQ=255)

                                                                                   tCGCCGCGTACTGGTATCGATAAGGAAATGGTTTTAAGTACATGCTTATTACCTATAAACAGGTTTAATTCTTTTTTTTTTCACTAACGTTTTTACCTCTTATTTTAAATTTTAGGTTAACCTAATTAATTTTCTAATaaa                                                     >  1:66105/1‑141 (MQ=255)

                                                                                           tACTGGTATCGATAAGGAAATGTTTTTTATTTTTTGTTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAat                                             >  2:1098733/1‑141 (MQ=255)

                                                                                           tACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAat                                             <  1:131888/141‑1 (MQ=255)

                                                                                            aCTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTAAGAata                                            >  2:588157/1‑141 (MQ=255)

                                                                                              tGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAtt                                          >  1:927518/1‑141 (MQ=255)

                                                                                               ggTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAttt                                         >  1:1168109/1‑141 (MQ=255)

                                                                                                gTATCGATAAGGAAATGTTTTTAAGTACCTGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTa                                        <  2:317753/141‑1 (MQ=255)

                                                                                                gTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTa                                        <  1:314914/141‑1 (MQ=255)

                                                                                                gTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTa                                        <  2:366340/141‑1 (MQ=255)

                                                                                                  aTCGATAAGGAAATGTTTTTAAATACATGCTTAATACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATc                                      <  1:947995/141‑1 (MQ=255)

                                                                                                        aaGGAAATTGTTGTAAGTACATGCTTATTACCTATAAAAAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCa                                <  2:614127/141‑1 (MQ=255)

                                                                                                         aGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCaa                               >  2:704908/1‑141 (MQ=255)

                                                                                                          gtaaaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                              <  2:1128578/139‑1 (MQ=255)

                                                                                                          ggAAATGTTTTTAAGTACATGGTTATTACCTATAACCAGATTTAAATCTTTTGTTTCCAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                              <  1:387755/141‑1 (MQ=255)

                                                                                                          ggAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                              <  1:68524/141‑1 (MQ=255)

                                                                                                          ggAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                              <  1:1150791/141‑1 (MQ=255)

                                                                                                          ggAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                              <  1:340989/141‑1 (MQ=255)

                                                                                                          agAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                              <  2:701465/140‑1 (MQ=255)

                                                                                                           gAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGt                             <  1:410364/141‑1 (MQ=255)

                                                                                                           gAAATGTTTTTAAGAACATGCTTATTACGTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAAATGAGAATATTTATCATGTCAAga                             >  1:786082/1‑140 (MQ=255)

                                                                                                           aaatggTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGt                             <  2:296609/136‑1 (MQ=255)

                                                                                                           aaaaTTTTTTTAAGTACATTCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGt                             <  2:897226/140‑1 (MQ=255)

                                                                                                            aaaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                            <  1:5579/141‑1 (MQ=255)

                                                                                                            aaaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                            <  2:885511/141‑1 (MQ=255)

                                                                                                             aaTTTTTTGAAGTACATGCTTATTACCTATACACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTCACCTAATTAATTGCATAATAAACTGAGAATATTTATCATGTCACGTaa                           <  2:833166/141‑1 (MQ=255)

                                                                                                             aaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaa                           <  1:588429/141‑1 (MQ=255)

                                                                                                              aTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                          <  2:139918/141‑1 (MQ=255)

                                                                                                              aTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                          <  2:102780/141‑1 (MQ=255)

                                                                                                              aTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                          <  1:422081/141‑1 (MQ=255)

                                                                                                              aTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTCATCATGTCAGGTaaa                          >  2:587236/1‑141 (MQ=255)

                                                                                                               tGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                         <  2:842170/141‑1 (MQ=255)

                                                                                                               tGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                         <  2:916551/141‑1 (MQ=255)

                                                                                                                ttttttAAGTACACGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAtt                        <  2:870307/140‑1 (MQ=255)

                                                                                                                  ttttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                      <  1:734952/141‑1 (MQ=255)

                                                                                                                  ttttAAGTACATGATTATTACCTATAAACAGATTGAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                      <  1:955053/141‑1 (MQ=255)

                                                                                                                   tttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTACTTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt                     <  2:655909/141‑1 (MQ=255)

                                                                                                                   tttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt                     <  1:857808/141‑1 (MQ=255)

                                                                                                                   tttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt                     >  1:1141046/1‑141 (MQ=255)

                                                                                                                    ttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTg                    >  1:490633/1‑141 (MQ=255)

                                                                                                                     tAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGa                   <  1:499588/141‑1 (MQ=255)

                                                                                                                     tAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGa                   >  1:81162/1‑141 (MQ=255)

                                                                                                                     tAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACATTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGa                   >  1:779202/1‑141 (MQ=255)

                                                                                                                         tACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatata               >  2:167399/1‑141 (MQ=255)

                                                                                                                            aTGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATGATGTCAAGTAAATTCGTGatataata            >  1:737645/1‑141 (MQ=255)

                                                                                                                               cgtattaCCTATAAACAGAGTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTAGCATGTCAAGTAAATTCGTGatataatataa         >  2:887712/3‑140 (MQ=255)

                                                                                                                               cTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAg         >  1:828658/1‑141 (MQ=255)

                                                                                                                                ttattaCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATTTCGAGGAAAGTAGTGATATAGTATAGa        >  1:212737/1‑141 (MQ=255)

                                                                                                                                ttattaCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGa        >  2:678123/1‑141 (MQ=255)

                                                                                                                                 tattaCCTATAAACAGATTTCAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCTTGATATAATATAGAc       <  1:892736/141‑1 (MQ=255)

                                                                                                                                 tattaCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGAc       <  1:390578/141‑1 (MQ=255)

                                                                                                                                     aCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg   <  2:892608/141‑1 (MQ=255)

                                                                                                                                      ccTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgt  <  1:1102496/141‑1 (MQ=255)

TACCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGT  >  USA300TCH1516_ALE/707741‑708015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`