BRESEQ :: Evidence

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 1205323	93 (0.820)	44 (0.530) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	10/200	11.3	38.6%	intergenic (‑204/‑365)	USA300HOU_RS15290/lspA	hypothetical protein/Lipoprotein signal peptidase
*	?	USA300TCH1516_ALE	2478252 =	100 (0.880)	44 (0.530) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	10/200	11.3	38.6%	intergenic (+139/‑436)	USA300HOU_RS12760/USA300HOU_RS12765	hypothetical protein/hypothetical protein
Rejected: Coverage evenness skew score above cutoff.

Only 100 of 102 total aligned reads displayed.

CCACAAACAAACTATTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTC  >  USA300TCH1516_ALE/2478115‑2478392

ccACAAACAAACTATTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAAca                                                                                                                                             <  1:599982/141‑1 (MQ=255)

 cacaAACAAACTATTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAAcag                                                                                                                                            >  1:658372/1‑141 (MQ=255)

    aaacaaacTATTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAAcagcag                                                                                                                                         >  2:458221/1‑141 (MQ=255)

      acaaacTATTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTTGGACATTAATCCCT‑AAAAAAAACAGCAGTa                                                                                                                                       <  2:700659/141‑1 (MQ=255)

       caaacTTTTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTaa                                                                                                                                      <  2:571364/141‑1 (MQ=255)

       caaacTATTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTaa                                                                                                                                      >  1:192996/1‑141 (MQ=255)

        aaacTAATATTAAAGGTTGTTTCTAATATTGCGACAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGCGACATTAATCCCTCAAAAAAAACAGCAGTaa                                                                                                                                      >  4:347402/1‑141 (MQ=255)

         aacTATTATTATAGGTTGTTTATAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATAAAAAAAA‑AAAAAAAACAGCAGTAAGa                                                                                                                                    <  2:661422/141‑1 (MQ=255)

           cTATTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATa                                                                                                                                  >  1:870219/1‑141 (MQ=255)

           cTATTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATa                                                                                                                                  <  1:307003/141‑1 (MQ=255)

           cTATTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATa                                                                                                                                  <  3:157040/141‑1 (MQ=255)

           cTATTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATa                                                                                                                                  <  1:135695/141‑1 (MQ=255)

            tattattatAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATCTAGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATaa                                                                                                                                 >  1:455280/1‑141 (MQ=255)

                attatAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATACCTAAAAAAAAACAGCAGTAAGATAAttt                                                                                                                              >  1:80010/1‑141 (MQ=255)

                  tataGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑a                                                                                                                          <  3:17993/141‑1 (MQ=255)

                     aGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATGGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑‑AAAAAAACAGCAGTAAGATAATTTTC‑AATTa                                                                                                                      >  1:436984/1‑141 (MQ=255)

                     aGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGGTAAGGCTTGATTAGACAATTTTGATATTTAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑‑AAAAAAACATCAGTAAGATAATTTTC‑AATTa                                                                                                                      >  1:436915/1‑141 (MQ=37)

                           tttCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGaaaa                                                                                                                 <  1:219738/141‑1 (MQ=255)

                                 atatTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCtt                                                                                                          >  4:83482/1‑141 (MQ=255)

                                   atTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTAc                                                                                                        <  1:637868/141‑1 (MQ=255)

                                     tGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTActg                                                                                                      >  2:205393/1‑141 (MQ=255)

                                      gcatCAAACAATCATTAAATATTCGTTAATCCTTGATTAGACAATTATTATAATGAAAATAAATAAACTAAAGAGTATGGTACATAAAAAAAA‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTActgc                                                                                                     <  2:201962/138‑1 (MQ=25)

                                       cGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTActgct                                                                                                    <  3:76647/141‑1 (MQ=255)

                                         tCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGt                                                                                                  >  1:333555/1‑141 (MQ=255)

                                         tCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGt                                                                                                  >  2:252332/1‑141 (MQ=255)

                                         tCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTTACTGCTGt                                                                                                  <  1:272872/141‑1 (MQ=37)

                                                  cTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTAttt                                                                                         <  1:128237/141‑1 (MQ=255)

                                                   tttAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTta                                                                                        >  1:279742/1‑141 (MQ=255)

                                                     tAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTtata                                                                                      <  1:513519/141‑1 (MQ=255)

                                                          atTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATa                                                                                 <  1:85449/141‑1 (MQ=255)

                                                          atTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATa                                                                                 <  1:154344/141‑1 (MQ=255)

                                                              gTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTc                                                                             <  3:226646/141‑1 (MQ=255)

                                                              gTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTc                                                                             >  2:858930/1‑141 (MQ=255)

                                                               ttAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCg                                                                            <  1:375934/141‑1 (MQ=255)

                                                                tAATCCGTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGt                                                                           <  1:503707/141‑1 (MQ=255)

                                                                    ccTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTg                                                                       <  1:1250/141‑1 (MQ=255)

                                                                    ccTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTg                                                                       >  1:706992/1‑141 (MQ=255)

                                                                    ccTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTg                                                                       >  2:583409/1‑141 (MQ=255)

                                                                    ccTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTg                                                                       >  3:253663/1‑141 (MQ=255)

                                                                        gATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATg                                                                   <  2:535980/141‑1 (MQ=255)

                                                                        gATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATg                                                                   <  1:532197/141‑1 (MQ=255)

                                                                        gATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATg                                                                   >  2:240374/1‑141 (MQ=255)

                                                                         aTTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATgg                                                                  <  2:711741/141‑1 (MQ=255)

                                                                         aTTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATgg                                                                  <  1:504493/141‑1 (MQ=255)

                                                                          ttAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGc                                                                 <  4:122438/141‑1 (MQ=255)

                                                                           tAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCt                                                                <  4:11775/141‑1 (MQ=255)

                                                                             gACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTc                                                              >  4:332188/1‑141 (MQ=255)

                                                                             gACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTc                                                              >  1:13172/1‑141 (MQ=255)

                                                                             gACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTc                                                              <  1:719701/141‑1 (MQ=255)

                                                                               cAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGc                                                            >  3:236816/1‑141 (MQ=255)

                                                                                 aTTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTa                                                          <  2:192996/141‑1 (MQ=255)

                                                                                  ttGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTAt                                                         >  1:448910/1‑141 (MQ=255)

                                                                                   tGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATg                                                        <  1:32829/141‑1 (MQ=255)

                                                                                      tataTTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGccc                                                     >  3:240482/1‑141 (MQ=255)

                                                                                       atatTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTCTTTATACAATACTTCGTATTGAATGACTTCGCTATGACCa                                                    >  1:480959/1‑141 (MQ=255)

                                                                                       atatTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCa                                                    <  3:231381/141‑1 (MQ=255)

                                                                                       atatTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCACTATGCCCa                                                    >  1:386938/1‑141 (MQ=255)

                                                                                          ttGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCt                                                 <  4:48442/141‑1 (MQ=255)

                                                                                          ttGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCt                                                 <  2:732845/141‑1 (MQ=255)

                                                                                               aataaataAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGcac                                            <  3:160692/141‑1 (MQ=255)

                                                                                                 taaataaaCTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACAt                                          <  1:248124/141‑1 (MQ=255)

                                                                                                 aaaaaaaaaTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTCTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTCCGTATTGAATGGCTTCGCTATGCCCATCTGGCACAt                                          <  2:103868/140‑1 (MQ=255)

                                                                                                  aaataaaCTAAAGAGTCTAGAACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACACTACTGCGTATTGAATGGCTTCGCTATGCCCATCTGGCACAtt                                         <  2:306622/141‑1 (MQ=255)

                                                                                                   aataaaCTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTtat                                                                                       >  1:212239/1‑94 (MQ=255)

                                                                                                   aataaaCTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTtat                                                                                       <  2:212239/94‑1 (MQ=255)

                                                                                                   aataaaCTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTa                                        >  2:41365/1‑141 (MQ=255)

                                                                                                    ataaaCTAAATAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTAc                                       <  2:540604/141‑1 (MQ=255)

                                                                                                    ataaaCTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTAc                                       <  1:252332/141‑1 (MQ=255)

                                                                                                      aaaCTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTg                                     >  1:780204/1‑141 (MQ=255)

                                                                                                       aaCTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGt                                    <  2:192164/141‑1 (MQ=255)

                                                                                                         cTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTaa                                  >  1:217962/1‑141 (MQ=255)

                                                                                                           aaaGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTaaaa                                <  1:754964/141‑1 (MQ=255)

                                                                                                           aaaGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCCGGCACATTACTGTaaaa                                >  2:555346/1‑141 (MQ=255)

                                                                                                             agagTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAAtt                              <  2:477273/141‑1 (MQ=255)

                                                                                                              gagTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATTGCTTCGCTATGCCCATCTGGCCCATTACTGTAAAAtct                             >  3:80079/1‑139 (MQ=255)

                                                                                                              gagTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTc                             >  4:156128/1‑141 (MQ=255)

                                                                                                                ggcTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACATTACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCta                           <  1:425030/139‑1 (MQ=255)

                                                                                                                 tCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCtat                          >  3:362815/1‑141 (MQ=255)

                                                                                                                  cTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCtata                         <  1:556017/141‑1 (MQ=255)

                                                                                                                   tGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATaa                        >  2:837913/1‑141 (MQ=255)

                                                                                                                   tGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATaa                        >  1:475315/1‑141 (MQ=255)

                                                                                                                    gggACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATaaa                       <  1:240374/141‑1 (MQ=255)

                                                                                                                    gggACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATaaa                       <  1:573250/141‑1 (MQ=255)

                                                                                                                     ggACCTTACTCCCT‑‑AAAAAAACAGCAGTCAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAACTTCtatttata                     <  2:436915/141‑6 (MQ=11)

                                                                                                                     ggACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAAt                      >  1:252684/1‑141 (MQ=255)

                                                                                                                     ggACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAAt                      <  2:346694/141‑1 (MQ=255)

                                                                                                                     ggACATTAATCCCT‑‑AAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCtatttata                     <  2:436984/141‑6 (MQ=255)

                                                                                                                      gACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATa                     <  2:96542/141‑1 (MQ=255)

                                                                                                                       aCATTAGTCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCTTAGTGTATTGCTTCTCTATGCCCATCTTGCACATTACTGTAGAATTCTATAGAtgg                    <  2:79262/141‑3 (MQ=14)

                                                                                                                        cATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGa                   <  2:870219/141‑1 (MQ=255)

                                                                                                                           tAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAAtt                <  1:3330/141‑1 (MQ=255)

                                                                                                                             aTCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACCTTACTGTAAAATTCTATAAATAGAAtttt              >  1:781904/1‑141 (MQ=255)

                                                                                                                               cccT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTtg            <  1:757840/141‑1 (MQ=255)

                                                                                                                                  t‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATTCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTtgatg         <  4:261689/141‑1 (MQ=255)

                                                                                                                                  t‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTtgatg         >  1:270907/1‑141 (MQ=40)

                                                                                                                                    aaaaaaaaCAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTtgatga        >  1:329573/1‑141 (MQ=38)

                                                                                                                                       aaaaaCAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACgg     >  4:221291/1‑141 (MQ=39)

                                                                                                                                        agaaCAGCAGTAAGATAATTTTT‑AATTAGAAAA‑TATCTTACTACTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGATggg    >  2:283625/3‑141 (MQ=255)

                                                                                                                                        aaaaCAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACggg    <  2:544266/141‑1 (MQ=38)

                                                                                                                                          aaCAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTc  >  1:46888/1‑141 (MQ=39)

CCACAAACAAACTATTATTATAGGTTGTTTCTAATATTGCGTCAAACTATCTTTAAATATTCGTTAATCCTTGATTAGACAATTGTTATATTGAAAATAAATAAACTAAAGAGTCTGGGACATTAATCCCT‑AAAAAAAACAGCAGTAAGATAATTTTC‑AATTAGAAAA‑TATCTTACTGCTGTTCTCTATTTATACAATACTTCGTATTGAATGGCTTCGCTATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTC  >  USA300TCH1516_ALE/2478115‑2478392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.