Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 2,289,252 C→A A80S (GCT→TCT)  cdaA ← Cyclic di‑AMP synthase CdaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,289,2520CA94.0% 348.0 / 16.0 116A80S (GCT→TCT) cdaACyclic di‑AMP synthase CdaA
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base A (58/51);  total (61/55)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

Only 100 of 117 total aligned reads displayed.

GCACAGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCG  >  USA300TCH1516_ALE/2289114‑2289389
                                                                                                                                          |                                                                                                                                         
   caGCCTTTGAAACCGCTTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGACAATa                                                                                                                                      >  3:325512/1‑141 (MQ=255)
   caGCCATTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCCAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGataata                                                                                                                                      <  3:262970/141‑1 (MQ=255)
     gCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATAcc                                                                                                                                    <  2:453002/141‑1 (MQ=255)
      ccTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATAccc                                                                                                                                   >  2:646494/1‑141 (MQ=255)
      ccTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATAccc                                                                                                                                   >  2:458903/1‑141 (MQ=255)
         ttGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATAcccccc                                                                                                                                <  1:811713/141‑1 (MQ=255)
         ttGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATAcccccc                                                                                                                                <  3:167411/141‑1 (MQ=255)
         ttGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATAcccccc                                                                                                                                >  4:242428/1‑141 (MQ=255)
         ttGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATAcccccc                                                                                                                                <  1:323286/141‑1 (MQ=255)
          tGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCa                                                                                                                               >  3:279172/1‑141 (MQ=255)
          cgAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCa                                                                                                                               >  3:283509/2‑141 (MQ=255)
           gAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCAt                                                                                                                              >  1:513983/1‑141 (MQ=255)
           gAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGCATTAGAAGTAGAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCAt                                                                                                                              <  2:163302/141‑1 (MQ=255)
               ccGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGaa                                                                                                                          >  2:274042/1‑141 (MQ=255)
                 gATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATa                                                                                                                        <  1:44226/141‑1 (MQ=255)
                  aTTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATaa                                                                                                                       >  2:540470/1‑141 (MQ=255)
                          aaTTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCg                                                                                                               >  3:345527/1‑141 (MQ=255)
                          aaTTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCg                                                                                                               <  1:140523/141‑1 (MQ=255)
                            tttCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTGAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGaa                                                                                                             <  1:447929/141‑1 (MQ=255)
                             ttCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGaat                                                                                                            <  3:189497/141‑1 (MQ=255)
                                 ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAAtt                                                                                                        >  3:303720/1‑141 (MQ=255)
                                 ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAAtt                                                                                                        >  3:20492/1‑141 (MQ=255)
                                 ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAAtt                                                                                                        >  2:209260/1‑141 (MQ=255)
                                 ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCAAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAAtt                                                                                                        >  1:418730/1‑141 (MQ=255)
                                  ttCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAAttt                                                                                                       >  1:197913/1‑141 (MQ=255)
                                    cATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTaa                                                                                                     >  3:40069/1‑140 (MQ=255)
                                    cATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAg                                                                                                     >  4:185067/1‑141 (MQ=255)
                                      tCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCACGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATACTACCCCCCATTGAATAACGATATCGAATAATTTAGAt                                                                                                   >  2:853423/1‑141 (MQ=255)
                                       cTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATg                                                                                                  >  2:142271/1‑141 (MQ=255)
                                        tttACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGt                                                                                                 <  2:352977/141‑1 (MQ=255)
                                        tttACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGt                                                                                                 <  2:101393/141‑1 (MQ=255)
                                         ttACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGtt                                                                                                >  1:14598/1‑141 (MQ=255)
                                          tACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTg                                                                                               <  1:233409/141‑1 (MQ=255)
                                           aCTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGc                                                                                              >  1:423510/1‑141 (MQ=255)
                                               taCGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTc                                                                                          >  1:456601/1‑141 (MQ=255)
                                                 cGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCaa                                                                                        >  1:794455/1‑141 (MQ=255)
                                                   tATTAGAAGTATAGCGTTTTAAAGAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTACAGATAATACCTCCCCTTGACTAACGATTTCGAATAATTTAGATGTTGCAGTCAAGt                                                                                      <  2:301954/141‑1 (MQ=255)
                                                     ttAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTc                                                                                    <  1:785236/141‑1 (MQ=255)
                                                     ttAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTACTTACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTc                                                                                    <  1:448038/141‑1 (MQ=255)
                                                      tAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCa                                                                                   <  1:553413/141‑1 (MQ=255)
                                                      tAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCa                                                                                   >  2:829946/1‑141 (MQ=255)
                                                       aGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCaa                                                                                  <  1:323722/141‑1 (MQ=255)
                                                        gAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGGTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAat                                                                                 >  1:760035/1‑141 (MQ=255)
                                                         aaGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAata                                                                                >  1:196490/1‑141 (MQ=255)
                                                          aGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAatat                                                                               <  1:74761/141‑1 (MQ=255)
                                                            tataGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCa                                                                             <  1:513838/141‑1 (MQ=255)
                                                             ataGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCAt                                                                            >  4:127252/1‑141 (MQ=255)
                                                               aGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATAc                                                                          <  3:137330/141‑1 (MQ=255)
                                                               aGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATAc                                                                          <  3:157268/141‑1 (MQ=255)
                                                                 cGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATACTAGTTCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACtt                                                                        >  1:455395/1‑141 (MQ=255)
                                                                 cGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACtt                                                                        >  3:166910/1‑141 (MQ=255)
                                                                  gTTTTAAAAAGCTACCTCTACCTAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTa                                                                       <  2:72670/141‑1 (MQ=255)
                                                                  gTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTa                                                                       <  1:141850/141‑1 (MQ=255)
                                                                    tttAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATc                                                                     >  2:184257/1‑141 (MQ=255)
                                                                              tACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCaa                                                           <  1:289520/141‑1 (MQ=255)
                                                                                 ctctACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATaa                                                        <  2:378433/141‑1 (MQ=255)
                                                                                        aaGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTAc                                                 <  2:142034/141‑1 (MQ=255)
                                                                                        aaGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCTTTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTAc                                                 <  2:461747/141‑1 (MQ=255)
                                                                                        aaGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTAc                                                 <  2:752625/141‑1 (MQ=255)
                                                                                         aGTTGTTCTAACGGACGACTGAATTCTGTTTGGAAGATTAGTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATACTTTACATGTTGCAATCAAGGTCACTATCATACTTATCTGCTTACCAATAACAATTACt                                                <  2:736665/141‑1 (MQ=255)
                                                                                         aGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACt                                                >  1:420654/1‑141 (MQ=255)
                                                                                          gttgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTa                                               >  2:469208/1‑141 (MQ=255)
                                                                                           ttgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTaa                                              >  1:212875/1‑141 (MQ=255)
                                                                                           ttgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTaa                                              >  2:244641/1‑141 (MQ=255)
                                                                                            tgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAat                                             >  1:570782/1‑141 (MQ=255)
                                                                                             gttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAACATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAata                                            >  4:220556/1‑141 (MQ=255)
                                                                                              gttctatgCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAatat                                           >  4:271783/8‑141 (MQ=255)
                                                                                               tCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATc                                          >  1:823015/1‑141 (MQ=255)
                                                                                               tCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATc                                          >  2:271081/1‑141 (MQ=255)
                                                                                                cTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATcc                                         >  1:548543/1‑141 (MQ=255)
                                                                                                 tAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATccc                                        >  1:179540/1‑141 (MQ=255)
                                                                                                   aCGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCtt                                      <  2:69837/141‑1 (MQ=255)
                                                                                                        cGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGt                                 >  3:335712/1‑141 (MQ=255)
                                                                                                         gTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAAt                                                          >  1:430049/1‑115 (MQ=255)
                                                                                                         gTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAAt                                                          <  2:430049/115‑1 (MQ=255)
                                                                                                            tAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTCGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGCAAtt                             >  1:142338/1‑141 (MQ=255)
                                                                                                            tAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAAtt                             <  1:577859/141‑1 (MQ=255)
                                                                                                              aTTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGt                           <  3:48346/141‑1 (MQ=255)
                                                                                                                ttCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTAt                         >  3:163307/1‑141 (MQ=255)
                                                                                                                ttCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTAt                         >  2:312712/1‑141 (MQ=255)
                                                                                                                   tGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGc                      >  2:566362/1‑141 (MQ=255)
                                                                                                                   tGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGc                      >  4:313425/1‑141 (MQ=255)
                                                                                                                   tGGTTGGAATATTACTATGAAAGATAATACCCCCCATTGAATGACGATAGCGAAGAATTTAGATGATGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAGCAATTACGAATATCCCTTTAAGTAATTGTAgcgc                      <  2:325135/141‑1 (MQ=255)
                                                                                                                      ttGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCttt                   <  2:860332/141‑1 (MQ=255)
                                                                                                                      ttGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCttt                   >  1:279508/1‑141 (MQ=255)
                                                                                                                        ggAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAg                 >  3:225697/1‑141 (MQ=255)
                                                                                                                         gAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGt                <  3:93168/141‑1 (MQ=255)
                                                                                                                           atatTACTATTAAAGAGAATACCCCCCATTGAATAACGATATAGAATCATTTAGATGTTGTAGTCAAGTTCAATATCATTCTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAACTAAGTGTATCGCTTTCGTTc              >  4:17311/1‑141 (MQ=255)
                                                                                                                            tatTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTcc             <  1:124733/141‑1 (MQ=255)
                                                                                                                            tatTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTcc             >  1:104450/1‑141 (MQ=255)
                                                                                                                             atTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTccc            >  2:45228/1‑141 (MQ=255)
                                                                                                                              ttACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCt           >  3:11850/1‑141 (MQ=255)
                                                                                                                              taaCTATTAAAAAAAATAACCCCCATTGAATAACGATATCGAATAAATTAAATGTTTCATTCTAGATCAATATCATACTTATTTACTTTCCTATAAAAATTACTAATATCCTTTCAGGTAATTTTATTGCTTTAGTTCCCt           >  4:226559/3‑141 (MQ=255)
                                                                                                                               tACTATTAAAGATAATACCCCCCATTGAATACCGCTATCGAATACGTCAGATCGTACAGTCAAGCTCAACATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCtt          <  1:780612/141‑1 (MQ=255)
                                                                                                                                 ctgttAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTaa        <  4:319924/138‑1 (MQ=255)
                                                                                                                                  tATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTaaa       <  2:669524/141‑1 (MQ=255)
                                                                                                                                     tAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGAc    <  2:570782/141‑1 (MQ=255)
                                                                                                                                      aaaGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGAcc   >  2:358636/1‑141 (MQ=255)
                                                                                                                                       aaGCTAATACCACCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCg  <  4:269024/141‑1 (MQ=255)
                                                                                                                                       aaGATAATACCCCCCCTTGAATAACGATATCGAATAATTTAGATGTTGCAGTTAAGTTCAATATCATACTTATCTGCTGACCAATATCAATTACTAATATCCCTTCAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCg  <  2:326943/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
GCACAGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCG  >  USA300TCH1516_ALE/2289114‑2289389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: