Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,360,799	A→G	G66G (GGT→GGC)	adk ←	Adenylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,360,799	0	A	G	96.8%	320.2 / 0.8	95	G66G (GGT→GGC)	adk	Adenylate kinase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (42/50);  total (44/51)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

TGACAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTG  >  USA300TCH1516_ALE/2360666‑2360934                                                                                                                                      |                                                                                                                                        tgaCAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAg                                                                                                                                  >  3:9104/1‑141 (MQ=255) ggaCAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAg                                                                                                                                  <  1:477728/140‑1 (MQ=255)   aCAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGTATTGTCTTTAGTAATTTTTTCCTTCACGATGCTTACAGTc                                                                                                                                >  2:141443/1‑141 (MQ=255)   aCAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTc                                                                                                                                <  4:303527/141‑1 (MQ=255)   aCAGCATAAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTc                                                                                                                                <  2:501742/141‑1 (MQ=255)     aGCATCAATGTTTCTGTCCAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCAc                                                                                                                              <  2:171963/141‑1 (MQ=255)         tCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTtca                                                                                                                          >  1:843523/1‑141 (MQ=255)           aaTGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTtcatc                                                                                                                        >  4:335028/1‑141 (MQ=255)            aTGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTtcatcc                                                                                                                       >  4:274417/1‑140 (MQ=255)              gTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTTTTTTTTTTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCTCTTCATCAgg                                                                                                                     >  2:354262/1‑141 (MQ=255)                ttCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGaa                                                                                                                   >  2:799033/1‑141 (MQ=255)                      cAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAAtt                                                                                                             >  1:865416/1‑141 (MQ=255)                       aaGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTc                                                                                                            >  4:190339/1‑141 (MQ=255)                             aGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACg                                                                                                      >  2:522042/1‑141 (MQ=255)                              gACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACgg                                                                                                     <  2:45352/141‑1 (MQ=255)                              gACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACgg                                                                                                     >  1:814461/1‑141 (MQ=255)                              gACATAATAGTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACgg                                                                                                     >  1:767336/1‑141 (MQ=255)                                cATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTc                                                                                                   <  1:133515/141‑1 (MQ=255)                                  taataTTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCTTCTTCTTCAGAAATTCTTTCCTTAACTATGTCTACAGTCACTTCATCAGGAACTAATTCACCACgcgcca                                                                                                 >  4:71095/1‑136 (MQ=255)                                    atatTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCata                                                                                               <  1:728453/141‑1 (MQ=255)                                           ttAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCC‑TTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACtt                                                                                       <  1:444282/141‑1 (MQ=255)                                            tAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACtt                                                                                       <  1:793004/141‑1 (MQ=255)                                              aTGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAg                                                                                     <  1:829601/141‑1 (MQ=255)                                              aTGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAg                                                                                     >  1:750761/1‑141 (MQ=255)                                               tGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGc                                                                                    <  1:298785/141‑1 (MQ=255)                                                gCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGTACTAATTCGCCACGGTCCATATAAGAATTAGct                                                                                   >  3:120056/1‑141 (MQ=255)                                                gCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGct                                                                                   <  1:107028/141‑1 (MQ=255)                                                  cTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGcttc                                                                                 <  1:341817/141‑1 (MQ=255)                                                   tCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGcttct                                                                                >  2:24689/1‑141 (MQ=255)                                                   tCAGCTTGCTCGATTGNTCTTGGGANGCCNTCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGcttct                                                                                >  1:765588/1‑141 (MQ=255)                                                      gCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTa                                                                             >  1:694519/1‑141 (MQ=255)                                                        ttGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTAcc                                                                           <  2:694519/141‑1 (MQ=255)                                                        ttGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTAcc                                                                           <  2:769022/141‑1 (MQ=255)                                                        ttGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTAcc                                                                           <  1:771857/141‑1 (MQ=255)                                                          gCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACCGTCCATATAAGACTTAGCTTCTTTACCTa                                                                         >  1:91551/1‑141 (MQ=255)                                                           cTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTaa                                                                        <  2:7627/141‑1 (MQ=255)                                                           cTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTaa                                                                        <  4:88168/141‑1 (MQ=255)                                                            tCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTaa                                                                        >  1:565928/1‑140 (MQ=255)                                                            tCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTaa                                                                        <  2:565928/140‑1 (MQ=255)                                                            tCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAAt                                                                       >  1:753939/1‑141 (MQ=255)                                                             cGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTCGCATCGTCTTAAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAAtt                                                                      >  3:209822/1‑141 (MQ=255)                                                                  gTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGtt                                                                 >  1:561291/1‑141 (MQ=255)                                                                     cTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTttct                                                              <  3:102907/141‑1 (MQ=255)                                                                     cTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTttct                                                              >  1:301620/1‑141 (MQ=255)                                                                     cTTGGGAAGCCATCAAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTttct                                                              <  2:605115/141‑1 (MQ=255)                                                                        gggAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTtcttct                                                           >  3:205857/1‑141 (MQ=255)                                                                        gggAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTtcttct                                                           >  3:28264/1‑141 (MQ=255)                                                                          gAAGCCATCTAATAAAAAGCCTTTTTTTTTTTTTTTTTCAGAAATTCTTTCCTTAACGATGCCTTCAGTCACTTCATCAGGAACTAATTCGCCACTGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCttt                                                         >  2:525914/1‑141 (MQ=255)                                                                           aaGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCtttt                                                        <  1:354262/141‑1 (MQ=255)                                                                            aGCCATCTAATAAAAAGCCTTTTTTTGTTTTTTTTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTta                                                       >  2:552389/1‑141 (MQ=255)                                                                             gCCCTCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTtat                                                      <  2:609064/141‑1 (MQ=255)                                                                             gCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTtat                                                      <  2:695554/141‑1 (MQ=255)                                                                             gCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTtat                                                      <  2:417334/141‑1 (MQ=255)                                                                                   taataaAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCttt                                                <  2:154203/141‑1 (MQ=255)                                                                                   taataaAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTACCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCttt                                                <  1:628423/141‑1 (MQ=255)                                                                                       aaaaaGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTg                                            <  1:103472/141‑1 (MQ=255)                                                                                         aaaGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGaa                                          >  2:69224/1‑141 (MQ=255)                                                                                          aaGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGaa                                                                                                                   >  2:347820/1‑67 (MQ=255)                                                                                          aaGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGaa                                                                                                                   <  1:347820/67‑1 (MQ=255)                                                                                          aaGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAAc                                         >  1:209103/1‑141 (MQ=255)                                                                                           aGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCCCTTAAAACAGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACa                                        <  1:15875/141‑1 (MQ=255)                                                                                           aGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCttt                                                                              <  2:171394/103‑1 (MQ=255)                                                                                           aGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCttt                                                                              >  1:171394/1‑103 (MQ=255)                                                                                             ccTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCa                                                                                                                               <  2:781968/52‑1 (MQ=255)                                                                                             ccTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCa                                                                                                                               >  1:781968/1‑52 (MQ=255)                                                                                             ccTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCGCGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATg                                      >  1:194597/1‑141 (MQ=255)                                                                                             ccTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATg                                      <  3:148763/141‑1 (MQ=255)                                                                                                  ttttGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCAcc                                 <  3:190339/141‑1 (MQ=255)                                                                                                   tttGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCa                                >  1:838023/1‑141 (MQ=255)                                                                                                    ttGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAg                               <  2:705963/141‑1 (MQ=255)                                                                                                     tGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGAAACTAATTCGCCACGGTACATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTTAAGATGtaaaaaga                              <  2:478826/141‑8 (MQ=255)                                                                                                      gCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGtt                             <  2:871031/141‑1 (MQ=255)                                                                                                       cATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTg                            <  3:335028/141‑1 (MQ=255)                                                                                                       cATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTg                            <  1:501487/141‑1 (MQ=255)                                                                                                            tCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAAtg                       <  1:522848/141‑1 (MQ=255)                                                                                                              ttCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAAtgtg                     <  2:317506/141‑1 (MQ=255)                                                                                                                 aGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTgggg                  <  1:516620/141‑1 (MQ=255)                                                                                                                 aGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTgggg                  <  1:516563/141‑1 (MQ=255)                                                                                                                     aTTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTGTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTAtt              <  2:395038/141‑1 (MQ=255)                                                                                                                       tCTTTCCTTAACGATGCCTACAGTCACTTCATCATGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTgg            <  2:210396/141‑1 (MQ=255)                                                                                                                       tCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTgg            <  2:819228/141‑1 (MQ=255)                                                                                                                       tCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTgg            >  2:634245/1‑141 (MQ=255)                                                                                                                       tCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTgg            >  4:93422/1‑141 (MQ=255)                                                                                                                       tCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTgg            >  1:65536/1‑141 (MQ=255)                                                                                                                        cTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTggg           >  1:439306/1‑141 (MQ=255)                                                                                                                         tttCCTTAACGATGCCTACAGTCACTTCATCAGGTACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGTACATGTCACCAGTTGAAATGTGGGGTATTTGGa          >  2:120819/1‑141 (MQ=255)                                                                                                                         tttCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGa          >  1:320350/1‑141 (MQ=255)                                                                                                                         tttCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGa          <  1:118396/141‑1 (MQ=255)                                                                                                                          ttCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGaa         >  4:206175/1‑141 (MQ=255)                                                                                                                          ttCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGaa         >  1:444173/1‑141 (MQ=255)                                                                                                                            ccTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAAtt       >  1:218865/1‑141 (MQ=255)                                                                                                                               tAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCt    <  1:524146/141‑1 (MQ=255)                                                                                                                               tAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCt    <  1:272332/141‑1 (MQ=255)                                                                                                                               tAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCt    <  1:641098/141‑1 (MQ=255)                                                                                                                                 aCGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTg  <  3:91425/141‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TGACAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTG  >  USA300TCH1516_ALE/2360666‑2360934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑